scholarly journals Utilizing a novel fungal enzymatic cocktail as an eco-friendly alternative for cellulose pulp biobleaching

BioResources ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. 7509-7529
Author(s):  
Vanessa Elisa Pinheiro ◽  
Jorge A. Ferreira ◽  
Jorge Henrique de Almeida Betini ◽  
Eliana Setsuko Kamimura ◽  
Maria de Lourdes Teixeira de Moraes Polizeli
Keyword(s):  
Cell Walls ◽  
Reducing Sugars ◽  
Toxic Chemicals ◽  
Optimal Conditions ◽  
Lignocellulosic Residues ◽  
Recycled Paper ◽  
Aspergillus Tamarii ◽  
Cellulose Pulp ◽  
Hydrolysis Of ◽  
Fungal Extracts

Enzyme cocktails can alter the lignin and hemicellulose content in wood cell walls, improving the bleaching process during pulp production and offsetting the need for toxic chemicals. In this study, brown pulp was biobleached with a mixture of crude fungal extracts rich in xylanase and laccase, respectively produced from Aspergillus tamarii Kita and Trametes versicolor on waste materials. The optimal conditions for biobleaching were a mixture of xylanase and laccase crude extracts (1 to 2 v/v), at a temperature of 36 °C and a pH of 5.5. The treated brown cellulose pulp showed a reduction in the Kappa number by 1.83 points, representing an efficiency of 20.3%. In addition, the brightness increased by 4.65 points in comparison to the control. Hence, studies involving the application of the standardized cocktail during the hydrolysis of lignocellulosic residues, e.g., barley residue and sugarcane bagasse, led to the formation of 85 g/L and 25 g/L of reducing sugars, respectively. Moreover, the standardized cocktail caused greater deinking of the recycled paper pulp.

Steam pretreatment for enzymatic hydrolysis of poplar wood: comparison of optimal conditions with and without SO2 impregnation

Holzforschung ◽  
2013 ◽  
Vol 67 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Fokko Schütt ◽  
Nils Peter Haas ◽  
Laura Dehne ◽  
Gerald Koch ◽  
Ron Janzon ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Cell Walls ◽  
Steam Pretreatment ◽  
Poplar Wood ◽  
Optimal Conditions ◽  
Steam Refining ◽  
Fiber Network ◽  
Lignin Distribution ◽  
Pretreatment Conditions ◽  
Hydrolysis Of

Abstract Steam refining of non-debarked poplar wood with SO2 impregnation prior to steaming was investigated as pretreatment for enzymatic hydrolysis. Pretreatment conditions were varied in the range of 170°C–220°C, 3–30 min and 0.7–2.5% SO2 according to a factorial design. Predicted steaming conditions for highest carbohydrate yields after enzymatic hydrolysis were at 200°C, 15 min, and 2.5% SO2. The yield of glucose and xylose from control tests under these conditions was 43% representing an increase of 9% compared to results of former experiments without SO2 impregnation. Investigations on lignin extracted from the fibers revealed no distinct differences between pretreatment with and without SO2. No sulfonation occurred by the impregnation with SO2. Topochemical analyses of the fibers by cellular UV microspectrophotometry (UMSP) showed an inhomogeneous lignin distribution within the S2 of fibers after pretreatment without SO2 and local depositions of high UV-absorbing substances in the lumina of fibers and parenchyma cells. The lignin distribution of fiber cell walls after pretreatment with SO2 was more homogeneous with a preserved fiber network and only little amounts of deposited phenolic compounds in the lumina. Therefore, it might be concluded that the expulsion of lignin hinders the enzymes in accessing the cellulose.

Deinking of recycled paper by coupling of the enzyme endo-β-1,4-D-glucanasa and the cellulose, and by using a laboratory flotation column

MRS Advances ◽  
2020 ◽  
Vol 5 (52-53) ◽  
pp. 2669-2678
Author(s):  
Jeovani González P. ◽  
Ramiro Escudero G
Keyword(s):  
Amino Acids ◽  
Sodium Hydroxide ◽  
Paper Industry ◽  
Computational Simulation ◽  
Cellulose Fibers ◽  
Residual Water ◽  
Chemical Reagent ◽  
Recycled Paper ◽  
Flotation Column ◽  
Hydrolysis Of

AbstractDeinking of recycled office (MOW) paper was carried out by using a flotation column and adding separately sodium hydroxide, and the enzyme Cellulase Thricodema Sp., as defibrillators.The de-inked cellulose fibers were characterized according to the standards of the paper industry, to compare the efficiency of the deinking of each chemical reagent used to hydrolyze the fibers and defibrillate them.The computational simulation of the molecular coupling between the enzyme and cellulose was performed, to establish the enzyme-cellulose molecular complex and then to identify the principal amino-acids of endo-β-1,4-D-glucanase in this molecular link, which are responsible for the hydrolysis of the cellulose.Experimental results show the feasibility to replace sodium hydroxide with the enzyme Cellulase Thricodema Sp., by obtaining deinked cellulose with similar optical and physical properties.The use of the enzyme instead of sodium hydroxide avoids the contamination of the residual water; in addition to that, the column is operated more easily, taking into consideration that the pH of the system goes from alkaline to neutral.

Enhanced Hydrolysis of Cellulose to Reducing Sugars on Kaolinte Clay Activated by Mineral Acid

2021 ◽  
Author(s):  
Yuxiao Dong ◽  
Dongshen Tong ◽  
Laibin Ren ◽  
Xingtao Chen ◽  
Hao Zhang ◽  
...  
Keyword(s):  
Reducing Sugars ◽  
Mineral Acid ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Base-catalyzed Degradations of Carbohydrates. IX. β-Eliminations of 4-O-Substituted Hexopyranosiduronates

1975 ◽  
Vol 53 (14) ◽  
pp. 2182-2188 ◽  
Author(s):  
Gerald O. Aspinall ◽  
Thinnayam N. Krishnamurthy ◽  
Walter Mitura ◽  
Masuo Funabashi
Keyword(s):  
Acid Hydrolysis ◽  
Reducing Sugars ◽  
Model Compounds ◽  
Methyl Methyl ◽  
Hydrolysis Of

Two methylated disaccharides, methyl [methyl 4-O-(2,3,4,6-tetra-O-methyl-α-D-glucopyranosyl)-2,3-di-O-methyl-β-D-glucopyranosid]uronate (9) and methyl 6-O-(methyl 2,3,4-tri-O-methyl-α-D-galactopyranosyluronate)-2,3,4-tri-O-methyl-β-D-glucopyranoside (15) have been synthesized and used as model compounds for the study of the base-catalyzed β-elimination of 4-O-substituted hexopyranosiduronates without degradation of exposed reducing sugars and of the selective acid hydrolysis of hex-4-enopyranosiduronates.

scholarly journals Production of Feruloyl Esterase from Aspergillus niger by Solid-State Fermentation on Different Carbon Sources

2011 ◽  
Vol 2011 ◽  
pp. 1-4 ◽  
Author(s):  
Shiyi Ou ◽  
Jing Zhang ◽  
Yong Wang ◽  
Ning Zhang
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Wheat Bran ◽  
Cell Walls ◽  
Carbon Sources ◽  
Feruloyl Esterase ◽  
Plant Cell Walls ◽  
Optimal Conditions ◽  
Carbon And Nitrogen ◽  
Maize Bran

A mixture of wheat bran with maize bran as a carbon source and addition of (NH4)SO4 as nitrogen source was found to significantly increase production of feruloyl esterase (FAE) enzyme compared with wheat bran as a sole carbon and nitrogen source. The optimal conditions in conical flasks were carbon source (30 g) to water 1 : 1, maize bran to wheat bran 1 : 2, (NH4)SO4 1.2 g and MgSO4 70 mg. Under these conditions, FAE activity was 7.68 mU/g. The FAE activity on the mixed carbon sources showed, high activity against the plant cell walls contained in the cultures.

Modeling and Optimization of Lipase-Catalyzed Partial Hydrolysis for Diacylglycerol Production in Packed Bed Reactor

2016 ◽  
Vol 12 (7) ◽  
pp. 681-689 ◽  
Author(s):  
Eng-Tong Phuah ◽  
Yee-Ying Lee ◽  
Teck-Kim Tang ◽  
Oi-Ming Lai ◽  
Thomas Shean-Yaw Choong ◽  
...  
Keyword(s):  
Immobilized Enzyme ◽  
Rhizomucor Miehei ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Partial Hydrolysis ◽  
Optimal Conditions ◽  
Lack Of Fit ◽  
Bed Height ◽  
Hydrolysis Of ◽  
Quadratic Models

Abstract Response surface methodology (RSM) was employed to optimize the process variables namely packed bed height (cm) and flow rates (ml/min) on diacylglycerol (DAG) production via partial hydrolysis of palm oil using immobilized Rhizomucor miehei lipase in packed bed reactor (PBR). Quadratic models were successfully developed for both DAG(y) and unhydrolyzed triacylglycerol ((un)TAG) with determination coefficient (R2) of 0.9931 and 0.9986, respectively coupled with insignificant lack of fit (p > 0.05). Optimal conditions for DAG synthesis were evaluated to be 10 cm packed bed height and 3.8 ml/min flow rate. Immobilized enzyme can be reused up to 10 times without significant changes in enzymatic activity. The partial hydrolysis under studied was found to be mass transfer-controlled.

scholarly journals Synergistic Hydrolysis of Carboxymethyl Cellulose and Acid-Swollen Cellulose by Two Endoglucanases (CelZ and CelY) fromErwinia chrysanthemi

2000 ◽  
Vol 182 (20) ◽  
pp. 5676-5682 ◽  
Author(s):  
Shengde Zhou ◽  
Lonnie O. Ingram
Keyword(s):  
Carboxymethyl Cellulose ◽  
Cell Walls ◽  
Cellulase Activity ◽  
Substrate Preference ◽  
Erwinia Chrysanthemi ◽  
General Mechanism ◽  
Plant Cell Walls ◽  
Enzymatic Modification ◽  
Amorphous Cellulose ◽  
Hydrolysis Of

ABSTRACT Erwinia chrysanthemi produces a battery of hydrolases and lyases which are very effective in the maceration of plant cell walls. Although two endoglucanases (CelZ and CelY; formerly EGZ and EGY) are produced, CelZ represents approximately 95% of the total carboxymethyl cellulase activity. In this study, we have examined the effectiveness of CelY and CelZ alone and of combinations of both enzymes using carboxymethyl cellulose (CMC) and amorphous cellulose (acid-swollen cellulose) as substrates. Synergy was observed with both substrates. Maximal synergy (1.8-fold) was observed for combinations containing primarily CelZ; the ratio of enzyme activities produced was similar to those produced by cultures of E. chrysanthemi. CelY and CelZ were quite different in substrate preference. CelY was unable to hydrolyze soluble cellooligosaccharides (cellotetraose and cellopentaose) but hydrolyzed CMC to fragments averaging 10.7 glucosyl units. In contrast, CelZ readily hydrolyzed cellotetraose, cellopentaose, and amorphous cellulose to produce cellobiose and cellotriose as dominant products. CelZ hydrolyzed CMC to fragments averaging 3.6 glucosyl units. In combination, CelZ and CelY hydrolyzed CMC to products averaging 2.3 glucosyl units. Synergy did not require the simultaneous presence of both enzymes. Enzymatic modification of the substrate by CelY increased the rate and extent of hydrolysis by CelZ. Full synergy was retained by the sequential hydrolysis of CMC, provided CelY was used as the first enzyme. A general mechanism is proposed to explain the synergy between these two enzymes based primarily on differences in substrate preference.

Valorization of agro-industrial wastes to produce hydrolytic enzymes by fungal solid-state fermentation

2018 ◽  
Vol 37 (2) ◽  
pp. 149-156 ◽  
Author(s):  
C. Marzo ◽  
A.B. Díaz ◽  
I. Caro ◽  
A. Blandino
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Reducing Sugars ◽  
Raw Materials ◽  
Hydrolytic Enzymes ◽  
Industrial Wastes ◽  
Added Value ◽  
Orange Peels ◽  
Enzyme Cocktail ◽  
Hydrolysis Of

Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, they represent interesting raw materials for the production of high-added value products. In this regard, orange peels (ORA) and exhausted sugar beet cossettes (ESBC) have turned out to be promising raw materials for hydrolytic enzymes production by solid state fermentation (SSF) and also a source of sugars which could be fermented to different high-added value products. The maximum activities of xylanase and exo-polygalacturonase (exo-PG) measured in the enzymatic extracts obtained after the SSF of ORA were 31,000 U·kg-1 and 17,600 U·kg-1, respectively; while for ESBC the maximum values reached were 35,000 U·kg-1 and 28,000 U·kg-1, respectively. The enzymatic extracts obtained in the SSF experiments were also employed for the hydrolysis of ORA and ESBC. Furthermore, it was found that extracts obtained from SSF of ORA, supplemented with commercial cellulase, were more efficient for the hydrolysis of ORA and ESBC than a commercial enzyme cocktail typically used for this purpose. In this case, maximum reducing sugars concentrations of 57 and 47 g·L-1 were measured after the enzymatic hydrolysis of ESBC and ORA, respectively.

Sign in / Sign up

Export Citation Format

Share Document