scholarly journals A New Spontaneous Allele at the Pink-Eyed Dilution (p) Locus Discovered in Mus musculus castaneus.

1995 ◽  
Vol 44 (4) ◽  
pp. 347-351 ◽  
Author(s):  
Atsushi TSUJI ◽  
Takao WAKAYAMA ◽  
Akira ISHIKAWA
Keyword(s):  
Mus Musculus ◽  
Pink Eyed Dilution ◽  
P Locus

Gene expression at the pink-eyed dilution (p) locus in the mouse is confirmed to be pigment cell autonomous using recombinant embryonic skin grafts

Development ◽  
1985 ◽  
Vol 87 (1) ◽  
pp. 65-73
Author(s):  
Dennis A. Stephenson ◽  
Janet E. Hornby
Keyword(s):  
Gene Expression ◽  
Environmental Influence ◽  
Pigment Cell ◽  
Epistatic Effect ◽  
Skin Grafts ◽  
Experimental Systems ◽  
Grafting Technique ◽  
Embryonic Skin ◽  
Pink Eyed Dilution ◽  
P Locus

Sash (Wsh), a viable and fully fertile allele of the dominant spotting (W) locus (Lyon & Glenister, 1982) has been expression at the pink-eyed dilution (p) locus. Reciprocal recombinant epidermal//dermal skin grafts were constructed from 13-day embryonic skin of p and Wsh homozygotes. Thus in the reciprocal experiments pink-eyed dilution melanocytes were exposed to any environmental influence from the wild-type allele of thep locus in either the epidermis (when WshWsh) or the dermis (when WshWsh). The hair pigmentation of the grafts recovered after three weeks beneath the testicular tunica of adult male mice was always typical of the p phenotype showing thatp is melanocyte autonomous. This result was supported by experiments using a modification of Mayer's (1965) neural crest grafting technique and the construction of 14-day recombinant skin grafts. Sash (WshWsh) epidermis can support melanocyte differentiation and pigment production but lacks functional melanocytes. The advantages of Wsh in experimental systems for testing the site of pigment gene expression have been demonstrated. Control experiments confirmed the dermal influence of agouti (A) over non-agouti (a) epidermis but non-agouti dermis did not overrule agouti pink-eyed dilution (AA pp) epidermis suggesting an epistatic effect ofp in the melanocyte.

scholarly journals The effects of mutant p-alleles on the reproductive system in mice

1974 ◽  
Vol 23 (3) ◽  
pp. 319-325 ◽  
Author(s):  
Roger W. Melvold
Keyword(s):  
Mus Musculus ◽  
Reproductive System ◽  
Pleiotropic Effects ◽  
Corpora Lutea ◽  
Gonadotropic Cells ◽  
Large Numbers ◽  
Pink Eyed Dilution

SUMMARYThe p6H, p25H, and pbs alleles at the pink-eyed dilution locus in mice (Mus musculus) cause sterility in males and semi-fertility in females when homozygous, while the pd, pun, p, and + alleles do not reduce fertility. Pituitaries from sterile males had significantly lower proportions of gonadotropic cells than pituitaries from fertile males. Ovaries from semi-fertile females contained large numbers of developing follicles, but no corpora lutea or corpora hemorrhagica were found. The proportion of polyovular follicles in ovaries of semifertile females was abnormally high.Pituitary gonadotropins appear to be reduced in the sterile genotypes although the lesion cannot be localized. Possible relationships between these and other pleiotropic effects of mutant p-alleles are discussed.

scholarly journals Genetic and molecular analysis of recessive alleles at the pink-eyed dilution (p) locus of the mouse.

1992 ◽  
Vol 89 (15) ◽  
pp. 6968-6972 ◽  
Author(s):  
M. F. Lyon ◽  
T. R. King ◽  
Y. Gondo ◽  
J. M. Gardner ◽  
Y. Nakatsu ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Pink Eyed Dilution ◽  
P Locus

scholarly journals Molecular analysis of 36 mutations at the mouse pink-eyed dilution (p) locus.

Genetics ◽  
1995 ◽  
Vol 141 (4) ◽  
pp. 1563-1571 ◽  
Author(s):  
D K Johnson ◽  
L J Stubbs ◽  
C T Culiat ◽  
C S Montgomery ◽  
L B Russell ◽  
...  
Keyword(s):  
Genetic Distances ◽  
Molecular Probes ◽  
Molecular Analyses ◽  
Deletion Mutations ◽  
Lethal Mutations ◽  
Isolation And Characterization ◽  
Chemically Induced ◽  
Pink Eyed Dilution ◽  
P Locus

Abstract Thirty-six radiation- or chemically induced homozygous-lethal mutations at the p locus in mouse chromosome 7 have been analyzed at 17 loci defined by molecular probes to determine the types of lesions, numbers of p-region markers deleted or rearranged, regions of overlap of deletion mutations, and genetic distances between loci. A linear deletion map of the [Myod1, Ldh3]-[Snrpn, Znf127] region has been constructed from the molecular analyses of the p-locus deletions. The utility of these deletions as tools for the isolation and characterization of the genes specifying the neurological, reproductive, and developmental phenotypes genetically mapped to this region will grow as more detailed molecular analyses continue.

scholarly journals Complementation analyses for 45 mutations encompassing the pink-eyed dilution (p) locus of the mouse.

Genetics ◽  
1995 ◽  
Vol 141 (4) ◽  
pp. 1547-1562 ◽  
Author(s):  
L B Russell ◽  
C S Montgomery ◽  
N L Cacheiro ◽  
D K Johnson
Keyword(s):  
Regulatory Elements ◽  
Large Deletion ◽  
Mouse Development ◽  
Coding Sequences ◽  
Fitness Functions ◽  
Neonatal Lethality ◽  
Radiation Induced ◽  
Pink Eyed Dilution ◽  
Compound Heterozygotes ◽  
P Locus

Abstract The homozygous and heterozygous phenotypes are described and characterized for 45 new pink-eyed dilution (p) locus mutations, most of them radiation-induced, that affect survival at various stages of mouse development. Cytogenetically detectable aberrations were found in three of the new p mutations (large deletion, inversion, translocation), with band 7C involved in each case. The complementation map developed from the study of 810 types of compound heterozygotes identifies five functional units: jls and jlm (two distinct juvenile-fitness functions, the latter associated with neuromuscular defects), pl-1 and pl-2 (associated with early-postimplantation and preimplantation death, respectively), and nl [neonatal lethality associated with cleft palate (the frequency of rare "escapers" from this defect varied with the genotype)]. Orientation of these units relative to genetic markers is as follows: centromere, Gas-2, pl-1, jls, jlm p, nl (equatable to cp 1 = Gabrb3); pl-2 probably resides in the c-deletion complex. pl-1 does not mask preimplantation lethals between Gas2 and p; and no genes affecting survival are located between p and cp1. The alleles specifying mottling or darker pigment (generically, pm and px, respectively) probably do not represent deletions of p-coding sequences but could be small rearrangements involving proximal regulatory elements.

Zwei Fallberichte einer disseminierten Toxoplasmose

2012 ◽  
Vol 40 (01) ◽  
pp. 64-69
Author(s):  
C. Kiesow ◽  
C. Ellenberger ◽  
B. Stief
Keyword(s):  
Mus Musculus ◽  
Ailurus Fulgens

ZusammenfassungEs werden die Fälle einer disseminierten letalen Toxoplasmose bei einer Farbmaus (Mus musculus) und einem Roten Panda (Ailurus fulgens) vorgestellt. Es handelte sich um eine als Haustier gehaltene Farbmaus und einen Roten Panda aus einem sächsischen zoologischen Garten. Die pathologische Untersuchung ergab bei beiden Tieren eine systemische Toxoplasmeninfektion. Eine hochgradige nekrotisierende Hepatitis stellte in beiden Fällen den histologischen Hauptbefund dar. Parasitenzysten fanden sich massenhaft in der Leber, in mäßiger Zahl im Gehirn und in geringer Zahl in anderen Organen. Mittels PAS-Reaktion waren diese Zysten bei der Farbmaus kaum darstellbar, beim Roten Panda dagegen sehr deutlich. PCR bzw. Immunhistologie bestätigten die Diagnose.

Effect of Thyroxinehormone on Blood Parameters of Pregnant and Non-Pregnant Females Mus musculus

2016 ◽  
Vol 29 (1) ◽  
pp. 172-184
Author(s):  
Sami J. Al-Maliki ◽  
Ali A. A. Al-Ali ◽  
Salma S. Abbas
Keyword(s):  
Mus Musculus ◽  
Blood Parameters ◽  
Pregnant Females

Analisis Kuantitatif Sel Purkinje Cerebellum Mencit (Mus musculus L.) setelah Induksi Ochratoksin A Selama Periode Organogenesis

2011 ◽  
Vol 16 (2) ◽  
Author(s):  
Arum Setiawan ◽  
Mammed Sagi ◽  
Widya Asmara ◽  
Istriyati Istriyati
Keyword(s):  
Mus Musculus

Penelitian ini bertujuan mengetahui jumlah sel Purkinje cerebellum anak mencit umur 21 hari (pascasapih) setelah induksi Ochratoksin A selama periode organogenesis. Tiga puluh ekor mencit bunting dibagi secara acak menjadi 5 kelompok perlakuan dengan masing-masing 6 ulangan. Ochratoksin A dilarutkan dalam Sodium Bicarbonat, diberikan secara oral pada saat kebuntingan hari ke 7 sampai hari ke -14. Dosis perlakuan Ochratoksin A adalah 0,5 ; 1,0; 1,5 mg/kg bb dan sebagai kontrol tidak diberi perlakuan, serta kontrol placebo diberi perlakuan pelarut Sodium Bicarbonat. Induk mencit dipelihara sampai melahirkan. Pada umur ke 21 hari (pascasapih), anak mencit dikorbankan dan diambil bagian otaknya. Otak mencit selanjutnya dipreparasi dengan metode parafin dan pewarnaan menggunakan pewarnaan Haematoksilin Eosin. Data jumlah sel Purkinje dianalisis dengan Anava Satu Arah dan dilanjutkan dengan uji DMRT untuk mengetahui beda nyata antar perlakuan. Hasil penelitian menunjukkan bahwa Ochratoksin A yang diberikan pada mencit bunting selama periode organogenesis menyebabkan terhambatnya pertumbuhan jumlah sel Purkinje mencit perlakuan yang ditandai dengan semakin menurunnya jumlah sel Purkinje dibandingkan dengan kontrol dan kontrol placebo.

Kerentanan Palatogenesis Mencit (Mus musculus L.) terhadap Induksi Cleft Palate TCDD

2011 ◽  
Vol 16 (2) ◽  
Author(s):  
Salomo Hutahaean ◽  
Soesanto Mangkoewidjojo ◽  
Mammed Sagi ◽  
Widya Asmara
Keyword(s):  
Cleft Palate ◽  
Mus Musculus ◽  
Cervical Dislocation

Telah dilakukan percobaan untuk menentukan tahapan palatogenesis pada mencit (Mus musculus L.) yang rentan terhadap efek polutan 2,3,7,8-Tetraklorodibenzo-p-dioksin (TCDD). Percobaan dirancang mengikuti Rancangan Acak Lengkap dengan pola faktorial (4X3). Empat puluh delapan ekor mencit bunting dicekok TCDD dengan dosis 0 (kontrol), 5, 10, atau 20 μg/kg bb. Perlakuan diberikan pada hari kebuntingan (Hk) 9−10, 11−12, atau 13−14. Mencit kontrol dicekok pelarut saja (98,5% minyak wijen + 1,5% DMSO). Pada Hk 18 mencit dibius lalu dibunuh dengan teknik cervical dislocation, persentase fetus cleft palate (cp) dihitung, derajat penutupan palatum diberi skor, preparat dengan ketebalan 6 µm dibuat, dan mikrostruktur kraniofasial diamati. Hasil menunjukkan, pemberian TCDD antara hari ke 9 dan 12 menginduksi cacat cp, dengan kecenderungan hasil tertinggi pada pemberian Hk 910. Perlakuan TCDD dosis 10 atau 20 μg/kg bb pada Hk 910 menghasilkan fetus cacat cp >90%. Persentase fetus cp tetap tinggi pada pemberian Hk 1112, khususnya pada kelompok dosis 20 μg/kg bb (87,3%). TCDD dosis terendah (5 μg/kg bb) menginduksi cp dominan bercelah sempit, menunjukkan adanya hambatan pada tahap fusi. Dosis 10 dan 20 μg/kg bb menginduksi cp bercelah sedang atau lebar, mengisyaratkan terjadi hambatan pada tahap inisiasi atau elevasi. Disimpulkan, seluruh tahapan palatogenesis rentan terhadap efek TCDD, namun tahap paling rentan adalah tahap fusi palatum.

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