Estimation of Genotoxic Effects from GSM Base Stations on Four Successive Generations (F1 F4) of Albino Mice, Mus Musculus Using RAPD Bands and Micronucleus Assay

2014 ◽  
Vol 2014 (2) ◽  
pp. 1-8
Author(s):  
Aneyo Ayisat ◽  
◽  
Otitoloju Akeem ◽  
Fowora Muinah ◽  
◽  
...  
Keyword(s):  
Mus Musculus ◽  
Micronucleus Assay ◽  
Base Stations ◽  
Genotoxic Effects ◽  
Albino Mice ◽  
Successive Generations

scholarly journals A Tool Derived from the Vicia faba Micronucleus Assay, to Assess Genotoxicity, Cytotoxicity or Biostimulation of Novel Compounds Used in Agriculture

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 321
Author(s):  
Perrine Klein ◽  
Lorelei Chauvey ◽  
Jean Kallerhoff ◽  
Eric Pinelli ◽  
Marie Morard ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Maleic Hydrazide ◽  
Lead Nitrate ◽  
Micronucleus Assay ◽  
Growth Potential ◽  
Protective Effects ◽  
Genotoxic Effects ◽  
Cytotoxic Effects ◽  
Conventional Agriculture ◽  
Rapid Tests

The increased use of biostimulants in conventional agriculture and organic farming requires the implementation of rapid tests to determine their effectiveness in enhancing plant growth and protection against abiotic stresses. However, their innocuity to plant health has rarely been demonstrated. We used the Vicia faba Micronucleus Assay, as described by the standard AFNOR EN ISO 29200(2020-05) to reveal biostimulant, genotoxic and cytotoxic effects of four commercialized wood-based products by comparing mitotic indices and micronucleus frequencies with respect to the controls. Neither genotoxicity, as measured by micronucleus frequency (MN), nor cytotoxicity, assessed by Mitotic index counts, was observed. Additionally, one of these stimulants (BHS®) conferred protective effects against contaminants (maleic hydrazide or lead nitrate). We describe that plotting micronuclei frequency against mitotic indices allows discrimination between cytotoxic/genotoxic effects from growth levels. Vicia faba experiments were successfully transposed to other agronomical important crops such as corn and sunflower. This technique can be valuable to industrials, to assess growth, potential cytoxicity and genotoxicity effects of any new biostimulant or organic.

scholarly journals Effect of Poly (N-IsoPropylAcrylamide) “PNIPAM” on Hepatic Cells of Swiss Albino Mice, Mus musculus

2013 ◽  
Vol 1 (4) ◽  
pp. 208-213 ◽  
Author(s):  
Md. Imtiyaz Aslam ◽  
Rohit Kumar Verma ◽  
Richa Roy ◽  
S. P. Roy
Keyword(s):  
Aqueous Solution ◽  
Mus Musculus ◽  
Quantitative Estimation ◽  
Polymeric Compound ◽  
Swiss Albino Mice ◽  
Hepatic Cells ◽  
Isopropyl Acrylamide ◽  
Albino Mice ◽  
Xenobiotic Compound ◽  
Histological Result

The present paper deals with the effect of polymeric compound poly (N-isopropyl acrylamide) “PNIPAM” for their toxicity on hepatic cells. The nanoparticle is a xenobiotic compound that accumulates in the liver for their metabolism. Non-metabolizing xenobiotic compounds such as PNIPAM produces anomalies in the hepatic cells. Certain enzymes such as ALT and ALP can be assayed for the hepatocytic toxicity.An attempt has been made to know the toxic effect of “PNIPAM” in a concentration of 0.8mg/ml on the hepatic cells of Swiss Albino mice, Mus musculus. The ALT and ALP analysis were performed through test kits for their quantitative estimation. The histological result shows that several lesions were produced after the introduction of aqueous solution of PNIPAM for an incubation period of 48 hours. The toxicity was confirmed Spectrophotometrically by the assessment of enzyme ALT and ALP. The increased concentration of ALT (55.0 IU/L) and slight decrease in ALP (40.0 IU/L) concentration was responsible for the metabolic alteration and production of hepatocytic anomalies in the mice.DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.9104    Int J Appl Sci Biotechnol, Vol. 1(4): 208-213

scholarly journals Arsenic induced genotoxic and histopathological changes in male Swiss albino mice, Mus musculus

2011 ◽  
Vol 3 (2) ◽  
pp. 329-339
Author(s):  
Animesh K. Mohapatra ◽  
Deepika Rai ◽  
Anika Tyagi
Keyword(s):  
Arsenic Trioxide ◽  
Mus Musculus ◽  
Seminiferous Tubules ◽  
Histopathological Changes ◽  
Histological Changes ◽  
Hydropic Degeneration ◽  
Swiss Albino Mice ◽  
Cytoplasmic Vacuolization ◽  
Liver And Kidney ◽  
Albino Mice

The present study was carried out to investigate the effect of arsenic trioxide on the DNA and histomorphology of testis, liver and kidney of Swiss albino mice, Mus musculus. Oral administration of arsenic trioxide induced DNA damage in the testis, liver and kidney marked by light pink staining of nuclei after Feulgen’s reaction with reduced fine chromatin. Simultaneously severe histological changes were noted like distortion of seminiferous tubules, disorganization of spermatogonia, spermatocytes and spermatids with cytoplasmic vacuolization and nuclear pycnosis in testis. There was almost disappearance of sinusoids due to disruption of hepatic plates, inflammatory cellular infiltration around central veins and cytoplasmic vacuolization in hepatocytes with large irregular nuclei in liver of treated mice. Disorganized glomeruli with distorted Bowman’s capsules and mild to severe multifocal cloudy and hydropic degeneration with necrosis of tubules were observed in the kidney of treated mice. Inference drawn from the study indicated that arsenic induced both genotoxic histotoxic lesions.

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