Kerentanan Palatogenesis Mencit (Mus musculus L.) terhadap Induksi Cleft Palate TCDD

2011 ◽  
Vol 16 (2) ◽  
Author(s):  
Salomo Hutahaean ◽  
Soesanto Mangkoewidjojo ◽  
Mammed Sagi ◽  
Widya Asmara
Keyword(s):  
Cleft Palate ◽  
Mus Musculus ◽  
Cervical Dislocation

Telah dilakukan percobaan untuk menentukan tahapan palatogenesis pada mencit (Mus musculus L.) yang rentan terhadap efek polutan 2,3,7,8-Tetraklorodibenzo-p-dioksin (TCDD). Percobaan dirancang mengikuti Rancangan Acak Lengkap dengan pola faktorial (4X3). Empat puluh delapan ekor mencit bunting dicekok TCDD dengan dosis 0 (kontrol), 5, 10, atau 20 μg/kg bb. Perlakuan diberikan pada hari kebuntingan (Hk) 9−10, 11−12, atau 13−14. Mencit kontrol dicekok pelarut saja (98,5% minyak wijen + 1,5% DMSO). Pada Hk 18 mencit dibius lalu dibunuh dengan teknik cervical dislocation, persentase fetus cleft palate (cp) dihitung, derajat penutupan palatum diberi skor, preparat dengan ketebalan 6 µm dibuat, dan mikrostruktur kraniofasial diamati. Hasil menunjukkan, pemberian TCDD antara hari ke 9 dan 12 menginduksi cacat cp, dengan kecenderungan hasil tertinggi pada pemberian Hk 910. Perlakuan TCDD dosis 10 atau 20 μg/kg bb pada Hk 910 menghasilkan fetus cacat cp >90%. Persentase fetus cp tetap tinggi pada pemberian Hk 1112, khususnya pada kelompok dosis 20 μg/kg bb (87,3%). TCDD dosis terendah (5 μg/kg bb) menginduksi cp dominan bercelah sempit, menunjukkan adanya hambatan pada tahap fusi. Dosis 10 dan 20 μg/kg bb menginduksi cp bercelah sedang atau lebar, mengisyaratkan terjadi hambatan pada tahap inisiasi atau elevasi. Disimpulkan, seluruh tahapan palatogenesis rentan terhadap efek TCDD, namun tahap paling rentan adalah tahap fusi palatum.

Twirler: A Mutant Affecting the Inner Ear of the House Mouse

Development ◽  
1958 ◽  
Vol 6 (1) ◽  
pp. 105-116
Author(s):  
Mary F. Lyon
Keyword(s):  
Cleft Palate ◽  
Inner Ear ◽  
House Mouse ◽  
Mus Musculus ◽  
Horizontal Plane ◽  
The Other ◽  
Genetic Tests ◽  
Postural Reflexes ◽  
New Born ◽  
Mode Of Inheritance

The mutant twirler, symbol Tw, of the house mouse, Mus musculus, was first recognized by the ‘waltzing’ behaviour which the heterozygotes show. It was later found to affect the morphology of the inner ear and, when homozygous, to cause death of new-born animals through harelip and cleft palate. This paper describes the behaviour and ear defects of heterozygotes and the appearance of homozygotes. It also describes the genetic tests carried out to determine the mode of inheritance. Twirler heterozygotes show head-shaking in a horizontal plane, combined often with circling, and less often with vertical head-shaking. Postural reflexes are abnormal. For example, normal mice of about a week old, if held up by the tail, respond to this change of position by extending the back and stretching the forelimbs forward. Twirler mice, on the other hand, flex the back, tuck the head under, and draw the limbs back.

Binucleate Spermiogenesis in the Mouse

1972 ◽  
Vol 30 ◽  
pp. 112-113
Author(s):  
John J. Wolosewick
Keyword(s):  
Electron Microscopy ◽  
Phosphate Buffer ◽  
Seminiferous Epithelium ◽  
Mouse Testis ◽  
Germinal Epithelium ◽  
Intercellular Bridges ◽  
Adult Mice ◽  
Cervical Dislocation ◽  
Human And Mouse

Classically, the male germinal epithelium is depicted as synchronously developing uninucleate spermatids conjoined by intercellular bridges. Recently, binucleate and multinucleate spermatids from human and mouse testis have been reported. The present paper describes certain developmental events in one type of binucleate spermatid in the seminiferous epithelium of the mouse.Testes of adult mice (ABP Jax) were removed from the animals after cervical dislocation and placed into 2.5% glutaraldehyde/Millonig's phosphate buffer (pH 7.2). Testicular capsules were gently split and separated, exposing the tubules. After 15 minutes the tissue was carefully cut into cubes (approx. 1mm), fixed for an additional 45 minutes and processed for electron microscopy.

Classroom Teacher and Speech Clinician Severity Ratings of Different Speech Disorders

1975 ◽  
Vol 6 (3) ◽  
pp. 119-124 ◽  
Author(s):  
Robert T. Wertz ◽  
Michael D. Mead
Keyword(s):  
Public School ◽  
Cleft Palate ◽  
Classroom Teacher ◽  
First Grade ◽  
Speech Disorders ◽  
Third Grade ◽  
Second Grade ◽  
Classroom Teachers

Typical examples of four different speech disorders—voice, cleft palate, articulation, and stuttering—were ranked for severity by kindergarten, first-grade, second-grade, and third-grade teachers and by public school speech clinicians. Results indicated that classroom teachers, as a group, moderately agreed with speech clinicians regarding the severity of different speech disorders, and classroom teachers displayed significantly more agreement among themselves than did the speech clinicians.

Holistic Communication Assessment for Young Children With Cleft Palate Using the International Classification of Functioning, Disability and Health:Children and Youth

2020 ◽  
Vol 51 (4) ◽  
pp. 914-938
Author(s):  
Anna Cronin ◽  
Sharynne McLeod ◽  
Sarah Verdon
Keyword(s):  
Young Children ◽  
Cleft Palate ◽  
Case History ◽  
Significant Others ◽  
International Classification ◽  
Assessment Tools ◽  
International Classification Of Functioning ◽  
Speech Language Pathologists ◽  
Holistic Assessment

Purpose Children with a cleft palate (± cleft lip; CP±L) can have difficulties communicating and participating in daily life, yet speech-language pathologists typically focus on speech production during routine assessments. The International Classification of Functioning, Disability and Health: Children and Youth Version (ICF-CY; World Health Organization, 2007 ) provides a framework for holistic assessment. This tutorial describes holistic assessment of children with CP±L illustrated by data collected from a nonclinical sample of seven 2- to 3-year-old children, 13 parents, and 12 significant others (e.g., educators and grandparents). Method Data were collected during visits to participants' homes and early childhood education and care centers. Assessment tools applicable to domains of the ICF-CY were used to collect and analyze data. Child participants' Body Functions including speech, language, and cognitive development were assessed using screening and standardized assessments. Participants' Body Structures were assessed via oral motor examination, case history questionnaires, and observation. Participants' Activities and Participation as well as Environmental and Personal Factors were examined through case history questionnaires, interviews with significant others, parent report measures, and observations. Results Valuable insights can be gained from undertaking holistic speech-language pathology assessments with children with CP±L. Using multiple tools allowed for triangulation of data and privileging different viewpoints, to better understand the children and their contexts. Several children demonstrated speech error patterns outside of what are considered cleft speech characteristics, which underscores the importance of a broader assessment. Conclusion Speech-language pathologists can consider incorporating evaluation of all components and contextual factors of the ICF-CY when assessing and working with young children with CP±L to inform intervention and management practices.

Compensatory Tongue-Palate-Posterior Pharyngeal Wall Relationships in Cleft Palate

1965 ◽  
Vol 30 (2) ◽  
pp. 166-173 ◽  
Author(s):  
Alta R. Brooks ◽  
Ralph L. Shelton ◽  
Karl A. Youngstrom
Keyword(s):  
Cleft Palate ◽  
Posterior Pharyngeal Wall ◽  
Pharyngeal Wall

Errata

1966 ◽  
Vol 31 (2) ◽  
pp. 208-208
Keyword(s):  
Cleft Palate ◽  
Pressure Ratio ◽  
Ration Group

In the February 1966 issue of this journal, two errors occurred in Joan C. Pitzner’s and Hughlett L. Morris’s article “Articulation Skills and Adequacy of Breath Pressure Ratios of Children with Cleft Palate.” On page 29, the heading “Reality” should be “Reliability,” and on page 30, the heading “Pressure-Ration Group One” should be “Pressure-Ratio Group One.”

Special Considerations for Evaluation and Treatment of Spanish-Speaking Patients with Cleft Palate

2016 ◽  
Vol 1 (5) ◽  
pp. 41-49
Author(s):  
Ellen Moore
Keyword(s):  
Cleft Palate ◽  
The United States ◽  
Culturally Competent ◽  
Speech Language Pathology ◽  
Spanish Speaking ◽  
Appropriate Treatment ◽  
Oral Pressure ◽  
Language Pathology ◽  
History Of ◽  
Spanish Speaking Population

As the Spanish-speaking population in the United States continues to grow, there is increasing need for culturally competent and linguistically appropriate treatment across the field of speech-language pathology. This paper reviews information relevant to the evaluation and treatment of Spanish-speaking and Spanish-English bilingual children with a history of cleft palate. The phonetics and phonology of Spanish are reviewed and contrasted with English, with a focus on oral pressure consonants. Cultural factors and bilingualism are discussed briefly. Finally, practical strategies for evaluation and treatment are presented. Information is presented for monolingual and bilingual speech-language pathologists, both in the community and on cleft palate teams.

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