Comparative mathematical analysis of functional properties of mice bone marrow in the phase of recovery of colony-forming units number after sub-lethal and repeated sub-lethal irradiation

Graft failure is a mortal complication in allogeneic bone marrow transplantation (BMT); T cells and natural killer cells are responsible for graft rejection. However, we have recently demonstrated that the recruitment of donor-derived stromal cells prevents graft failure in allogeneic BMT. This finding prompted us to examine whether a major histocompatibility complex (MHC) restriction exists between hematopoietic stem cells (HSCs) and stromal cells. We transplanted bone marrow cells (BMCs) and bones obtained from various mouse strains and analyzed the cells that accumulated in the engrafted bones. Statistically significant cell accumulation was found in the engrafted bone, which had the same H-2 phenotype as that of the BMCs, whereas only few cells were detected in the engrafted bones of the third-party H-2 phenotypes during the 4 to 6 weeks after BMT. Moreover, the BMCs obtained from the MHC-compatible bone showed significant numbers of both colony-forming units in culture (CFU-C) and spleen colony-forming units (CFU-S). These findings strongly suggest that an MHC restriction exists between HSCs and stromal cells.

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Thermal Sensitivity and Thermally Enhanced Radiosensitivity of Murine Bone Marrow Granulocyte-Macrophage Colony-Forming Units(CFU-GM).

Thermal Medicine(Japanese Journal of Hyperthermic Oncology) ◽

10.3191/thermalmedicine.10.65 ◽

1994 ◽

Vol 10 (1) ◽

pp. 65-77

Author(s):

Hiroshi Yoshida

Keyword(s):

Bone Marrow ◽

Thermal Sensitivity ◽

Murine Bone Marrow ◽

Colony Forming Units ◽

Macrophage Colony

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Hematopoietic Recovery after Transplantation CD117+B220- (lacZ+) Bone Marrow Cells in Lethally Irradiated Mice

Acta Medica (Hradec Kralove Czech Republic) ◽

10.14712/18059694.2017.6 ◽

2008 ◽

Vol 51 (1) ◽

pp. 37-41 ◽

Cited By ~ 4

Author(s):

Miroslav Hodek ◽

Jiřina Vávrová ◽

Zuzana Šinkorová ◽

Jaroslav Mokrý ◽

Stanislav Filip

Keyword(s):

Bone Marrow ◽

Bone Marrow Cells ◽

Repair Process ◽

Whole Body ◽

Lacz Gene ◽

Colony Forming Units ◽

The Difference ◽

Macrophage Colony ◽

Marrow Cells

Experiments presented here were aimed at the description of hematopoiesis repair and in vivo homing of transplanted separated CD117+B220–bone marrow cells after whole-body lethal irradiation at LD 9Gy. ROSA 26 mice were used as donors of marrow cells for transplantation [B6;129S/Gt (ROSA)26Sor] and were tagged with lacZ gene, and F2 hybrid mice [B6129SF2/J] were used as recipients of bone marrow transplanted cells. Hematopoiesis repair was provided by transplantation, both suspension of whole bone marrow cells (5x106) and isolated CD117+B220–cells (5x104). Mice survived up to thirty days after irradiation. We demonstrated that transplantation of suspension of whole bone marrow cells led to faster recovery of CFU-GM (Granulocyte-macrophage colony forming units) in bone marrow and in the spleen too. It is not clear what the share of residential and transplanted cells is in the repair process. Our results demonstrate that sufficient hematopoietic repair occurs after transplantation of CD117+B220–(lacZ+) in lethally irradiated mice, and the difference in CFU-GM numbers in the bone marrow and spleen found on day 8 posttransplant has no influence on the survival of lethally irradiated mice (30 days follow-up).

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Preliminary Study on the Anti-radiation Effect of Jen-Sheng-Yang-Yung-Tang

The American Journal of Chinese Medicine ◽

10.1142/s0192415x93000224 ◽

1993 ◽

Vol 21 (02) ◽

pp. 187-195 ◽

Cited By ~ 7

Author(s):

Hsue-yin Hsu ◽

Yau-hui Ho ◽

Shi-Iong Lian ◽

Chun-ching Lin

Keyword(s):

Bone Marrow ◽

Radiation Effect ◽

Bone Marrow Cells ◽

X Rays ◽

Male Mice ◽

X Ray ◽

Colony Forming Units ◽

Before And After ◽

Preliminary Study ◽

Different Doses

Six to seven week old male mice of ICR strain were exposed to different doses of x-rays to determine if Jen-Sheng-Yang-Yung-Tang could be a modifier in the elimination of radiation damage. Colony forming units of bone marrow cells in the spleen (CFUs) were measured before and after x-ray irradiation with intraperitoneal injection of 10 mg/20 g or 20 mg/20 g body weight of Jen-Sheng-Yang-Yung-Tang, once a day for seven consecutive days. The recovery of CFUs and hemocytes counts by 4 Gy irradiation with Jen-Sheng-Yang-Yung-Tang administration was faster for a concentration of 20 mg/20 g than 10 mg/20 g. The measurement of 10-day CFUs showed an increase of radiotolerance in the treatment of 20 mg/20 g administration before x-ray irradiation. The injection of Jen-Sheng-Yang-Yung-Tang accelerated the recovery of hemocyte counts in mice irradiated with 4 Gy x-ray; the effect was especially profound for leukocytes with 20 mg/20 g Jen-Sheng-Yang-Yung-Tang administration after irradiation.

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