Study on fibroblast colony forming units derived from bone marrow of normal adults and untreated patients with acute nonlymphocytic leukemia

1988 ◽  
Vol 8 (2) ◽  
pp. 87-88
Author(s):  
Chen Yan ◽  
Li Chong-yu ◽  
Wang Bian-ming ◽  
Shen Di ◽  
Yu Dong-jiao
Keyword(s):  
Bone Marrow ◽  
Acute Nonlymphocytic Leukemia ◽  
Colony Forming Units ◽  
Normal Adults

Vertebral bone marrow diffusivity in normal adults with varying bone densities at 3T diffusion-weighted imaging

2017 ◽  
Vol 59 (1) ◽  
pp. 89-96 ◽  
Author(s):  
Jie He ◽  
Hao Fang ◽  
Xiao na Li
Keyword(s):  
Bone Marrow ◽  
Bone Density ◽  
Diffusion Weighted Imaging ◽  
Mineral Density ◽  
Vertebral Bone ◽  
Normal Bone ◽  
Diffusion Weighted ◽  
Adc Values ◽  
Vertebral Bone Marrow ◽  
Normal Adults

Background There has been controversy surrounding the relationship between diffusivity and bone mineral density (BMD) in vertebral bone marrow. Moreover, sex-related differences of vertebral bone marrow diffusivity in relation to varying bone densities have not yet been evaluated. Purpose To prospectively investigate the role of diffusion-weighted imaging (DWI) in assessing vertebral marrow changes in normal adults with varying bone densities. Material and Methods A total of 124 normal adult volunteers were enrolled in this study. Sagittal magnetic resonance (MR) DWI of the lumbar spine was performed. The ADC values of vertebral bone marrow were measured. Volumetric BMD measurement was performed by quantitative computed tomography (QCT) using Mindways QCT analysis software. All participants were divided into three groups according to BMD (normal, osteopenia, osteoporosis). The differences of the apparent diffusion coefficient (ADC) values of the three groups was compared, and partial correlation analysis was used to evaluate the correlation between ADC values and BMD. Results ADC values decreased as BMD decreased in female participants. When compared with the normal bone density group, ADC values were significantly decreased in the osteoporotic group and in the osteopenic group of female participants. ADC values of female participants were significantly higher than of male participants in the normal bone density group ( P < 0.001). ADC values correlated positively with BMD values (r = 0.307, P = 0.016) for female participants. Conclusion The diffusivity in vertebral bone marrow with varying bone densities differed by sex. ADC values correlated positively with BMD in women. DWI can quantitively evaluate osteoporosis in women.

scholarly journals Major Histocompatibility Complex Restriction Between Hematopoietic Stem Cells and Stromal Cells In Vivo

Blood ◽  
1997 ◽  
Vol 89 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Futoshi Hashimoto ◽  
Kikuya Sugiura ◽  
Kyoichi Inoue ◽  
Susumu Ikehara
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Major Histocompatibility Complex ◽  
Hematopoietic Stem Cells ◽  
Stromal Cells ◽  
Graft Failure ◽  
Major Histocompatibility ◽  
Hematopoietic Stem ◽  
Histocompatibility Complex ◽  
Colony Forming Units

Graft failure is a mortal complication in allogeneic bone marrow transplantation (BMT); T cells and natural killer cells are responsible for graft rejection. However, we have recently demonstrated that the recruitment of donor-derived stromal cells prevents graft failure in allogeneic BMT. This finding prompted us to examine whether a major histocompatibility complex (MHC) restriction exists between hematopoietic stem cells (HSCs) and stromal cells. We transplanted bone marrow cells (BMCs) and bones obtained from various mouse strains and analyzed the cells that accumulated in the engrafted bones. Statistically significant cell accumulation was found in the engrafted bone, which had the same H-2 phenotype as that of the BMCs, whereas only few cells were detected in the engrafted bones of the third-party H-2 phenotypes during the 4 to 6 weeks after BMT. Moreover, the BMCs obtained from the MHC-compatible bone showed significant numbers of both colony-forming units in culture (CFU-C) and spleen colony-forming units (CFU-S). These findings strongly suggest that an MHC restriction exists between HSCs and stromal cells.

scholarly journals Hematopoietic Recovery after Transplantation CD117+B220- (lacZ+) Bone Marrow Cells in Lethally Irradiated Mice

2008 ◽  
Vol 51 (1) ◽  
pp. 37-41 ◽  
Author(s):  
Miroslav Hodek ◽  
Jiřina Vávrová ◽  
Zuzana Šinkorová ◽  
Jaroslav Mokrý ◽  
Stanislav Filip
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Repair Process ◽  
Whole Body ◽  
Lacz Gene ◽  
Colony Forming Units ◽  
The Difference ◽  
Macrophage Colony ◽  
Marrow Cells

Experiments presented here were aimed at the description of hematopoiesis repair and in vivo homing of transplanted separated CD117+B220–bone marrow cells after whole-body lethal irradiation at LD 9Gy. ROSA 26 mice were used as donors of marrow cells for transplantation [B6;129S/Gt (ROSA)26Sor] and were tagged with lacZ gene, and F2 hybrid mice [B6129SF2/J] were used as recipients of bone marrow transplanted cells. Hematopoiesis repair was provided by transplantation, both suspension of whole bone marrow cells (5x106) and isolated CD117+B220–cells (5x104). Mice survived up to thirty days after irradiation. We demonstrated that transplantation of suspension of whole bone marrow cells led to faster recovery of CFU-GM (Granulocyte-macrophage colony forming units) in bone marrow and in the spleen too. It is not clear what the share of residential and transplanted cells is in the repair process. Our results demonstrate that sufficient hematopoietic repair occurs after transplantation of CD117+B220–(lacZ+) in lethally irradiated mice, and the difference in CFU-GM numbers in the bone marrow and spleen found on day 8 posttransplant has no influence on the survival of lethally irradiated mice (30 days follow-up).

Preliminary Study on the Anti-radiation Effect of Jen-Sheng-Yang-Yung-Tang

1993 ◽  
Vol 21 (02) ◽  
pp. 187-195 ◽  
Author(s):  
Hsue-yin Hsu ◽  
Yau-hui Ho ◽  
Shi-Iong Lian ◽  
Chun-ching Lin
Keyword(s):  
Bone Marrow ◽  
Radiation Effect ◽  
Bone Marrow Cells ◽  
X Rays ◽  
Male Mice ◽  
X Ray ◽  
Colony Forming Units ◽  
Before And After ◽  
Preliminary Study ◽  
Different Doses

Six to seven week old male mice of ICR strain were exposed to different doses of x-rays to determine if Jen-Sheng-Yang-Yung-Tang could be a modifier in the elimination of radiation damage. Colony forming units of bone marrow cells in the spleen (CFUs) were measured before and after x-ray irradiation with intraperitoneal injection of 10 mg/20 g or 20 mg/20 g body weight of Jen-Sheng-Yang-Yung-Tang, once a day for seven consecutive days. The recovery of CFUs and hemocytes counts by 4 Gy irradiation with Jen-Sheng-Yang-Yung-Tang administration was faster for a concentration of 20 mg/20 g than 10 mg/20 g. The measurement of 10-day CFUs showed an increase of radiotolerance in the treatment of 20 mg/20 g administration before x-ray irradiation. The injection of Jen-Sheng-Yang-Yung-Tang accelerated the recovery of hemocyte counts in mice irradiated with 4 Gy x-ray; the effect was especially profound for leukocytes with 20 mg/20 g Jen-Sheng-Yang-Yung-Tang administration after irradiation.

A critical comparison of allogeneic bone marrow transplantation and conventional chemotherapy as treatment for acute nonlymphocytic leukemia.

1984 ◽  
Vol 2 (5) ◽  
pp. 369-378 ◽  
Author(s):  
C B Begg ◽  
P B McGlave ◽  
J M Bennett ◽  
P A Cassileth ◽  
M M Oken
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Marrow Transplantation ◽  
Disease Free Survival ◽  
Acute Nonlymphocytic Leukemia ◽  
Conventional Chemotherapy ◽  
Free Survival ◽  
Critical Comparison ◽  
Selection Factors ◽  
Disease Free

Published data from two centers conducting bone marrow transplantation on patients with acute nonlymphocytic leukemia in first remission were pooled and compared with results from an Eastern Cooperative Oncology Group (ECOG) study in which patients were treated with conventional chemotherapy. A series of adjustments were made to the ECOG sample to account for selection factors that restrict access of patients to transplantation. The transplant sample exhibits considerably higher disease-free survival when compared to the adjusted ECOG series (53% versus 21% at three years). The transplant series is somewhat younger than the ECOG series (median, 24 years versus 28 years). The impact of age on the disease-free survival results is difficult to assess because of the relatively small samples in the different age groups. However, by defining a suitable control group, methodology for making a critical comparison between the two modalities is presented which, if applied to larger samples of patients, should help to resolve the issue. In the absence of data from a large, prospective randomized study, a critical retrospective comparison of available data is essential in the assessment of treatment options.

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