scholarly journals Вплив стимуляторів росту на ініціацію експлантів Thuja Occidentalis L. в умовах in vitro

2019 ◽  
Vol 29 (5) ◽  
pp. 47-50
Author(s):  
M. M. Lisoviy
Keyword(s):  
Thuja Occidentalis ◽  
Skoog Medium ◽  
Murashige And Skoog Medium

На основі критичного аналізу літературних джерел встановлено потребу проведення поглибленого дослідження процесу росту експлантів Thuja occidentalis L. на етапі ініціації під час мікроклонального розмноження в умовах in vitro під впливом різних стимуляторів росту. Розглянуто застосовану методику виконання експериментальних досліджень. Проведено тестування трьох рецептів базових живильних середовищ: Murashige and Skoog medium (MS), Risser and White medium (RW) та Litvay medium (LM) і встановлено, що для мікроклонування досліджуваного виду оптимальним є RW. Визначено вплив синтетичних фітогормонів ауксинового (2,4-D та НОК) і цитокінінового (БАП) типу на успішність проходження етапу ініціації експлантів Thuja occidentalis L. Досліджено явище утворення калюсної тканини в експлантів під впливом різних ростових речовин. Виконано якісну оцінку ініціації експлантів, на основі якої проведено їхній поділ на три групи: "незадовільна ініціація", "задовільна ініціація" та "добра ініціація", залежно від збільшення лінійних розмірів рослинного матеріалу. Встановлено, що оптимальним живильним середовищем для ініціації росту досліджуваного виду є RW, модифіковане 0,3 мг/л (2,4-D)+0,5 мг/л (НОК)+0,2 мг/л (БАП).

In vitro plant regeneration from leaf expiants of Lycopersicon esculentum (tomato)

1976 ◽  
Vol 54 (21) ◽  
pp. 2409-2414 ◽  
Author(s):  
R. M. Behki ◽  
S. M. Lesley
Keyword(s):  
Plant Regeneration ◽  
Lycopersicon Esculentum ◽  
Growth Regulators ◽  
In Vitro Plant Regeneration ◽  
Leaf Discs ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Napthaleneacetic Acid ◽  
In Vitro Plant

Leaf discs from 15 mutant clones of tomato were tested for their morphogenetic response in Murashige and Skoog medium supplemented with 12 combinations of the growth regulators napthaleneacetic acid (NAA) and benzylaminopurine (BA) and 4 combinations of NAA and zeatin. The results show that either callus, shoots, roots, or shoots and roots can be produced depending upon the hormone concentrations and ratios. Plants were regenerated from 12 of the 15 varieties tested.

scholarly journals Effects of Cytokinin on in vitro Propagation of Bauhinia variegata L.

2020 ◽  
Vol 1 (6) ◽  
Author(s):  
Belai Meeta Suwal Singh
Keyword(s):  
In Vitro Propagation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Nodal Cuttings ◽  
Half Strength ◽  
Bauhinia Variegata ◽  
Mature Seeds

Mature seeds of Bauhinia variegata L were cultured on half strength Murashige and Skoog medium. For experimentation, nodal cuttings were used as explants from in vitro growing plants. Cytokinin, N-benzyl-9-(2-tetrahydropyranyl) (BPA), kinetin(6-furfurylaminopurine), zeatin, 6-(4-hydroxy-3-methyl-trans -2-butenyl amino purine), 2- isopentenyl amino purine (2-ip), and benzylaminopurine (BAP) were tested for best propagation. Well grown plants were achieved in medium supplemented with 5 µM BPA and 0.5 µM BAP. The propagated plants were acclimatized very well after transferred to the field.

scholarly journals Axillary Shoot Proliferation from in Vitro Culture of Pulmonaria L. Shoots

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 630d-630
Author(s):  
Dennis P. Stimart ◽  
John C. Mather
Keyword(s):  
In Vitro Culture ◽  
Shoot Proliferation ◽  
Axillary Shoot ◽  
Optimum Level ◽  
Axillary Shoot Proliferation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Rooting Response

Actively growing shoots from Pulmonaria L. `Roy Davidson' were cultured in vitro on Murashige and Skoog medium containing benzyladenine (BA) to establish proliferating cultures. BA at 0, 0.4, 0.8, 4.4, 8.8, and 44.4 μm was compared for shoot proliferation and rooting response. Shoot count was highest on 8.8 μm BA with root count highest on 0 or 0.4 μm BA. Subculture 4 weeks later of shoots to the same treatments resulted in highest shoot counts on 44.4 μm BA. Optimum level for micropropagation was 8.8 or 44.4 μm BA. Greatest rooting was at 0 or 0.4 μm BA.

scholarly journals In vitro micropropagation in Boehmeria nivea to generate safe planting materials for large-scale cultivation

2018 ◽  
Vol 54 (No. 4) ◽  
pp. 183-189
Author(s):  
Pranit Kumar Mukherjee ◽  
Raju Mondal ◽  
Sourav Dutta ◽  
Kanti Meena ◽  
Madhumita Roy ◽  
...  
Keyword(s):  
Large Scale ◽  
Random Amplified Polymorphic Dna ◽  
Ectopic Expression ◽  
Skoog Medium ◽  
Boehmeria Nivea ◽  
In Vitro Micropropagation ◽  
Murashige And Skoog Medium ◽  
Planting Materials ◽  
Large Scale Cultivation

An efficient in vitro micropropagation protocol has been developed using nodal explants of ramie (Boehmeria nivea), with maximum shoots (42) per explant in 5 passages (passage duration: 21 days) on Murashige and Skoog medium supplemented with 2.0 mg/l 6-benzyladenine and 2.0 mg/l AgNO<sub>3</sub>. ½ Murashige and Skoog medium containing 40% sucrose was found to be most effective for the rooting of in vitro developed shoots. Those plantlets were acclimatized and transferred to pots for hardening under glasshouse conditions. About 91% of mericlones survived and showed no ectopic expression in respect of any morphological character in comparison with the parental stock. Furthermore, clonal fidelity of the mericlones was confirmed by using DNA markers (random amplified polymorphic DNA and inter simple sequence repeats) and by polypeptide profiling through SDS-PAGE at a genomic and protein level, respectively, which showed the true-to-type nature of the in vitro micropropagated plants. Thus the protocol developed can be used to generate safe planting material for large-scale cultivation of ramie.  

Optimization of biotechnological process clonal micropropagation in vitro of Asparagus officinalis L.

2021 ◽  
Vol 12 (3) ◽  
Author(s):  
Y Kolomiiets ◽  
◽  
A Skuba ◽  
Keyword(s):  
Culture Medium ◽  
Asparagus Officinalis ◽  
Skoog Medium ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Regenerated Plants ◽  
Murashige And Skoog Medium ◽  
Macro And Micronutrients ◽  
Indole 3 Acetic Acid

The study presents the results of obtaining regenerated plants of asparagus from seeds. Surface sterilizing the seeds by 0,75% sodium hypochlorite for 30 min is effective, during this obtained 83% viable sterile plants. The Murashige and Skoog medium supplemented with 6‑benzylaminopurine (2 mg/L), inositol (100 mg/L) and thiamine (0,4 mg/L) was found to be the best for seed germination. The expediency of using kinetin (1 mg/L) as a growth regulator to obtain a homogeneous plant material was established. The reproduction coefficient was 6,0. Only 11% of the explants formed callus. For the selection needs and production of somaclonal variants, the use of the culture medium with indole-3-acetic acid (0,2 mg/L) and 6‑benzylaminopurine (1 mg/L) is justified. In this condition reproduction coefficient was 3,7, and the level of different intensity callusogenesis was 59%. The rooting of obtained plants was performed in Murashige and Skoog medium supplemented with a half dose of macro- and micronutrients and growth regulators. Rooting frequency was up to 63%. The knowledge of hormonal requirements helps to promote isolated tissue and cells technologies of asparagus with purpose of rapid propagation and obtaining healthy, high-quality planting material.

MICROPROPAGATION OF Helianthus maximiliani (Schrader) BY SHOOT APEX CULTURE / MICROPROPAGACION DE Helianthus maximiliani (Schrader) POR EL CULTIVO DE VAINAS DE LOS VASTAGOS / MICROPROPAGATION DE L’Helianthus maximiliani (Schrader) AU MOYEN DE LA CULTURE DE L’APEX DE LA POUSSE

Helia ◽  
2001 ◽  
Vol 24 (34) ◽  
pp. 63-68 ◽  
Author(s):  
Vasić Dragana ◽  
Škorić Dragan ◽  
Alibert Gilbert ◽  
Miklič Vladimir
Keyword(s):  
Silver Nitrate ◽  
Shoot Apex ◽  
Casein Hydrolysate ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Shoot Apices ◽  
Skoog Medium ◽  
Leaf Mesophyll ◽  
Murashige And Skoog Medium

SUMMARYH.maximiliani was micropropagated using culture of shoot apices on modified Murashige and Skoog medium (DV). Further propagation of in vitro grown plants was done by culture of their nodal segments and shoot tips on the same medium supplemented with phloridzin, silver nitrate and casein hydrolysate (DV'). Rooting was induced by dipping the explants into IBA solution prior culture. Viable protoplasts (90%) were isolated from leaf mesophyll. These protoplasts divided (18%) in culture in agarose droplets.

scholarly journals Use of growth inhibitor chlormequat chloride in potato culture in vitro

2021 ◽  
Vol 285 ◽  
pp. 03003
Author(s):  
Sergey A. Buldakov
Keyword(s):  
Nutrient Medium ◽  
Growth Inhibitor ◽  
Test Tube ◽  
Investigational Product ◽  
Chlormequat Chloride ◽  
Skoog Medium ◽  
Potato Culture ◽  
Murashige And Skoog Medium ◽  
The Cost

The publication presents data on the use of growth inhibitor chlormequat chloride in in vitro potato culture on varieties of different maturity groups: Meteor (early), Zekura (mid-early), and Northern Lights (mid-season). Five dosages of the investigational product were studied, ranging from 0.1125 to 1.8 g/l. It was found that in maximum doses of the product there was a strong inhibition of all growth processes in all varieties. The research results showed that the most optimal concentration of chlormequat chloride is 0.225 g per 1 liter of Murashige and Skoog medium. At this dose, on 30th day of cultivation, there was a decrease in the height of microplants from the control by 63.2-85.1%, in the root length - up to 15.0% and their number - up to 22.8% and an increase in the number of internodes by 6.5-22,0 % depending on the variety. The investigational product had an effect on formation of microtubers; in the Meteor variety, their largest number was 89.5% in the nutrient medium with a dose of 1.8 g/l, in the Zekura variety - 93.0% in the nutrient medium with a dose of 0.9 g/l. The new technique makes it possible to lengthen the periods between cuttings of test-tube plants by 2.3 times. This, in turn, reduces the cost of maintaining the in vitro collection material of potatoes in the summer-autumn period, and improves its quality, since each additional cutting cycle affects a more rapid degeneration of the variety. Also, microplants grown with growth inhibitor during subsequent relocation to a standard Murashige and Skoog medium did not show an aftereffect.

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