scholarly journals Use of growth inhibitor chlormequat chloride in potato culture in vitro

2021 ◽  
Vol 285 ◽  
pp. 03003
Author(s):  
Sergey A. Buldakov
Keyword(s):  
Nutrient Medium ◽  
Growth Inhibitor ◽  
Test Tube ◽  
Investigational Product ◽  
Chlormequat Chloride ◽  
Skoog Medium ◽  
Potato Culture ◽  
Murashige And Skoog Medium ◽  
The Cost

The publication presents data on the use of growth inhibitor chlormequat chloride in in vitro potato culture on varieties of different maturity groups: Meteor (early), Zekura (mid-early), and Northern Lights (mid-season). Five dosages of the investigational product were studied, ranging from 0.1125 to 1.8 g/l. It was found that in maximum doses of the product there was a strong inhibition of all growth processes in all varieties. The research results showed that the most optimal concentration of chlormequat chloride is 0.225 g per 1 liter of Murashige and Skoog medium. At this dose, on 30th day of cultivation, there was a decrease in the height of microplants from the control by 63.2-85.1%, in the root length - up to 15.0% and their number - up to 22.8% and an increase in the number of internodes by 6.5-22,0 % depending on the variety. The investigational product had an effect on formation of microtubers; in the Meteor variety, their largest number was 89.5% in the nutrient medium with a dose of 1.8 g/l, in the Zekura variety - 93.0% in the nutrient medium with a dose of 0.9 g/l. The new technique makes it possible to lengthen the periods between cuttings of test-tube plants by 2.3 times. This, in turn, reduces the cost of maintaining the in vitro collection material of potatoes in the summer-autumn period, and improves its quality, since each additional cutting cycle affects a more rapid degeneration of the variety. Also, microplants grown with growth inhibitor during subsequent relocation to a standard Murashige and Skoog medium did not show an aftereffect.

scholarly journals Future outlook of plant growth regulators application influencing callus induction from different type explant Hyoscyamus muticus L. in vitro

2018 ◽  
Vol 7 (3) ◽  
pp. 10-13
Author(s):  
Walla Abdelmaksood Abdelazeez ◽  
Landysh Zavdetovna Khusnetdinova ◽  
Olga Arnoldovna Timofeeva
Keyword(s):  
Callus Induction ◽  
Nutrient Medium ◽  
Callus Formation ◽  
Stem Explants ◽  
Hyoscyamus Muticus ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Naphthylacetic Acid ◽  
Egyptian Henbane

The article shows the results concerning the problem of the influence of the hormonal composition of the medium on callus induction in isolated from different explants of Egyptian henbane areas (on the example of Hyoscyamus muticus L.). The authors study 11 variants of Murashige and Skoog medium supplemented with different concentrations and combination of auxins and cytokinins. It was important to find nutrient medium modification of Murashige and Skoog for callus induction. The article describes the fact that callus formation from different explant types of Hyoscyamus muticus L. in vitro was observed on Murashige and Skoog medium fortified with benzylaminopurine and naphthylacetic acid. It shows that the maximum callus induction was observed from root explants on Murashige and Skoog's medium supplemented with 0.5 mg/l of benzylaminopurine and 1.0 mg/l of naphthylacetic acid. And minimal callus formation was observed in the area with benzylaminopurine. Callus induction of leaf and stem explants both on the hormone-free nutrient medium and with the benzylaminopurine only was not observed. Thus, the results show that the frequency of callus formation with culturing root segment is higher compared to leaf and stem segment explants (on the example of Egyptian henbane in culture in vitro ). This work aims to inducing callus formation from various explants of Egyptian henbane, which can be used for plant regeneration or as a source for in vitro production of secondary metabolites.

In vitro plant regeneration from leaf expiants of Lycopersicon esculentum (tomato)

1976 ◽  
Vol 54 (21) ◽  
pp. 2409-2414 ◽  
Author(s):  
R. M. Behki ◽  
S. M. Lesley
Keyword(s):  
Plant Regeneration ◽  
Lycopersicon Esculentum ◽  
Growth Regulators ◽  
In Vitro Plant Regeneration ◽  
Leaf Discs ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Napthaleneacetic Acid ◽  
In Vitro Plant

Leaf discs from 15 mutant clones of tomato were tested for their morphogenetic response in Murashige and Skoog medium supplemented with 12 combinations of the growth regulators napthaleneacetic acid (NAA) and benzylaminopurine (BA) and 4 combinations of NAA and zeatin. The results show that either callus, shoots, roots, or shoots and roots can be produced depending upon the hormone concentrations and ratios. Plants were regenerated from 12 of the 15 varieties tested.

scholarly journals Effects of Cytokinin on in vitro Propagation of Bauhinia variegata L.

2020 ◽  
Vol 1 (6) ◽  
Author(s):  
Belai Meeta Suwal Singh
Keyword(s):  
In Vitro Propagation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Nodal Cuttings ◽  
Half Strength ◽  
Bauhinia Variegata ◽  
Mature Seeds

Mature seeds of Bauhinia variegata L were cultured on half strength Murashige and Skoog medium. For experimentation, nodal cuttings were used as explants from in vitro growing plants. Cytokinin, N-benzyl-9-(2-tetrahydropyranyl) (BPA), kinetin(6-furfurylaminopurine), zeatin, 6-(4-hydroxy-3-methyl-trans -2-butenyl amino purine), 2- isopentenyl amino purine (2-ip), and benzylaminopurine (BAP) were tested for best propagation. Well grown plants were achieved in medium supplemented with 5 µM BPA and 0.5 µM BAP. The propagated plants were acclimatized very well after transferred to the field.

scholarly journals Propagation of Lily Arumsari using Generic Media through In Vitro Culture

2020 ◽  
Vol 11 (2) ◽  
pp. 140-148
Author(s):  
Ridho Kurniati ◽  
Fauziah Khairatunnisa ◽  
Reni Indrayanti
Keyword(s):  
Plant Growth Regulator ◽  
Low Cost ◽  
Ms Medium ◽  
Single Factor ◽  
Mass Propagation ◽  
Murashige And Skoog Medium ◽  
Number Of Leaves ◽  
Leaves And Roots ◽  
The Cost

Lily was usually propagated using MS (Murashige and Skoog) medium supplemented with plant growth regulator and hormone. The important concerns in mass propagation and seed production of lily for industries are cheap, easy and low cost medium. This study’s objective was to find out of generic media to substitute MS medium and to decrease the cost of mass propagation of lily in vitro. Three substitutions medium were Vitagrow, Growmore, Gibril, and G-60. Bulbs of lily Arumsari varieties were used as materials. Complete Random Design with a single factor was used in the experimental design. The treatments were type of generic in vitro medium (Vitagrow, Growmore, Gibril, and G-60), consisting of three replication and 20 units per repetition and five bulbs per unit of repetition. The parameter observed was the total number of leaves and roots, length of leaves and roots, and bulb growth percentage. Growmore medium showed a better result than others in total number of leaves (50,67), length of leaves (1,6 cm), length of roots (0,312 cm) and percentage of bulb growth (100%). The highest total number of roots was achieved in G-60 medium.

scholarly journals Вплив стимуляторів росту на ініціацію експлантів Thuja Occidentalis L. в умовах in vitro

2019 ◽  
Vol 29 (5) ◽  
pp. 47-50
Author(s):  
M. M. Lisoviy
Keyword(s):  
Thuja Occidentalis ◽  
Skoog Medium ◽  
Murashige And Skoog Medium

На основі критичного аналізу літературних джерел встановлено потребу проведення поглибленого дослідження процесу росту експлантів Thuja occidentalis L. на етапі ініціації під час мікроклонального розмноження в умовах in vitro під впливом різних стимуляторів росту. Розглянуто застосовану методику виконання експериментальних досліджень. Проведено тестування трьох рецептів базових живильних середовищ: Murashige and Skoog medium (MS), Risser and White medium (RW) та Litvay medium (LM) і встановлено, що для мікроклонування досліджуваного виду оптимальним є RW. Визначено вплив синтетичних фітогормонів ауксинового (2,4-D та НОК) і цитокінінового (БАП) типу на успішність проходження етапу ініціації експлантів Thuja occidentalis L. Досліджено явище утворення калюсної тканини в експлантів під впливом різних ростових речовин. Виконано якісну оцінку ініціації експлантів, на основі якої проведено їхній поділ на три групи: "незадовільна ініціація", "задовільна ініціація" та "добра ініціація", залежно від збільшення лінійних розмірів рослинного матеріалу. Встановлено, що оптимальним живильним середовищем для ініціації росту досліджуваного виду є RW, модифіковане 0,3 мг/л (2,4-D)+0,5 мг/л (НОК)+0,2 мг/л (БАП).

scholarly journals Axillary Shoot Proliferation from in Vitro Culture of Pulmonaria L. Shoots

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 630d-630
Author(s):  
Dennis P. Stimart ◽  
John C. Mather
Keyword(s):  
In Vitro Culture ◽  
Shoot Proliferation ◽  
Axillary Shoot ◽  
Optimum Level ◽  
Axillary Shoot Proliferation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Rooting Response

Actively growing shoots from Pulmonaria L. `Roy Davidson' were cultured in vitro on Murashige and Skoog medium containing benzyladenine (BA) to establish proliferating cultures. BA at 0, 0.4, 0.8, 4.4, 8.8, and 44.4 μm was compared for shoot proliferation and rooting response. Shoot count was highest on 8.8 μm BA with root count highest on 0 or 0.4 μm BA. Subculture 4 weeks later of shoots to the same treatments resulted in highest shoot counts on 44.4 μm BA. Optimum level for micropropagation was 8.8 or 44.4 μm BA. Greatest rooting was at 0 or 0.4 μm BA.

scholarly journals In vitro micropropagation in Boehmeria nivea to generate safe planting materials for large-scale cultivation

2018 ◽  
Vol 54 (No. 4) ◽  
pp. 183-189
Author(s):  
Pranit Kumar Mukherjee ◽  
Raju Mondal ◽  
Sourav Dutta ◽  
Kanti Meena ◽  
Madhumita Roy ◽  
...  
Keyword(s):  
Large Scale ◽  
Random Amplified Polymorphic Dna ◽  
Ectopic Expression ◽  
Skoog Medium ◽  
Boehmeria Nivea ◽  
In Vitro Micropropagation ◽  
Murashige And Skoog Medium ◽  
Planting Materials ◽  
Large Scale Cultivation

An efficient in vitro micropropagation protocol has been developed using nodal explants of ramie (Boehmeria nivea), with maximum shoots (42) per explant in 5 passages (passage duration: 21 days) on Murashige and Skoog medium supplemented with 2.0 mg/l 6-benzyladenine and 2.0 mg/l AgNO<sub>3</sub>. ½ Murashige and Skoog medium containing 40% sucrose was found to be most effective for the rooting of in vitro developed shoots. Those plantlets were acclimatized and transferred to pots for hardening under glasshouse conditions. About 91% of mericlones survived and showed no ectopic expression in respect of any morphological character in comparison with the parental stock. Furthermore, clonal fidelity of the mericlones was confirmed by using DNA markers (random amplified polymorphic DNA and inter simple sequence repeats) and by polypeptide profiling through SDS-PAGE at a genomic and protein level, respectively, which showed the true-to-type nature of the in vitro micropropagated plants. Thus the protocol developed can be used to generate safe planting material for large-scale cultivation of ramie.  

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