scholarly journals КОНТРОЛЬ ЛІПОФІЛЬНИХ ТОКСИКАНТІВ У МОЛОЦІ З ВИКОРИСТАННЯМ МІКРОБІОЛОГІЧНОГО ЕКСПРЕС–МЕТОДУ

2016 ◽  
Vol 18 (3(71)) ◽  
pp. 186-189
Author(s):  
M.S. Khimich ◽  
O.M. Gorobey ◽  
V.Z. Salata
Keyword(s):  
Test Organism ◽  
Laboratory Animals ◽  
Investigational Product ◽  
Vital Functions ◽  
Test Culture ◽  
Colpoda Steinii ◽  
Leachate Solution ◽  
Combined Action ◽  
Skimmed Milk ◽  
Trading Network

In recent years, Ukraine is constantly increasing livestock production. But at the same time, in many modern industries, veterinary and sanitary control of food is on insufficient or non–existent. In this connection the trading network can get dangerous foods for consumers.Today in the world there are numerous number of methods of analysis of food safety (spectrometry, photometry, chromatography, etc.). But in our country, a network of specialized laboratories which would be able to provide high–quality and objective analysis of the food is still very limited.It is also important to remember that the current chemical–analytical methods unfortunately unable to answer the general reaction of the organism to influence the recipient of the object, which is associated with several factors: the test object may contain toxicants with content during the study not predicted poorly understood substances, and importantly, is not considered character combined action of toxicants. That despite these shortcomings and remains an important method of determining the toxicity bioassay.The aim of our research was to establish the feasibility and effectiveness of the rapid microbiological method using ciliates Colpoda steinii to determine the toxicity of skimmed milk.Toxicity studies were carried out in parallel by three methods: bioassay in laboratory animals (white mice) and microbiological using as a test organism ciliates Tetrachimena piriformis and Colpoda steinii.The proposed method of using as a test organism ciliates Colpoda steinii, including the preparation of test culture ciliates Colpoda steinii, sampling investigational product, extracting samples of chemically pure acetone, filtering selected extract dilution leachate solution rod–Lozinski, introducing the filtrate into a test culture ciliates, temperature control resulting mixture at a temperature of +26 ... + 28 °C and determine the toxicity of the investigational product, observing the vital functions of ciliates during incubation.The research found that data on the toxicity of skimmed milk a certain prescribed manner identical data obtained using classical methods.Established that the strong toxicity of skimmed milk in the application of research results ciliates C. steinii get already at 3min. And weak toxicity or netoksychnosti – 1 hour. 

scholarly journals STUDY OF ANTIGENOTOXIC POTENTIAL OF THE ROSEHIP (ROSA MAJALIS HERRM.) OF THE FAMILY ROSACEAE

REPORTS ◽  
2021 ◽  
Vol 2 (336) ◽  
pp. 54-60
Author(s):  
Zh. A. Zhonderbek ◽  
S. Zh. Kolumbayeva ◽  
A. V. Lovinskaya ◽  
N. Voronova
Keyword(s):  
Bone Marrow ◽  
Adverse Effects ◽  
Environmental Pollutants ◽  
The Body ◽  
Laboratory Animals ◽  
Laboratory Mice ◽  
Herbal Tea ◽  
The Family ◽  
Induced Mutagenesis ◽  
Combined Action

Increasing the body's resistance to various environmental pollutants' adverse effects is one of medicine's essential tasks. In this regard, an active search for antimutagens to eliminate or weaken mutagens' effect in the body is currently underway. One of the promising sources of antimutagenic compounds is the medicinal plant Rosa majalis Herrm (rosehips). The genotoxic and antigenotoxic activity of rosehips was studied on cells of bone marrow, spleen, liver, and kidneys of laboratory mice using an alkaline variant Comet assay. It was found that rosehip infusions in various concentrations (infusion, diluted infusion and herbal tea) do not have a genotoxic effect on the cells of the studied organs of laboratory animals. The medicinal rosehip's combined action with classical mutagen MMS significantly reduced (p<0.01) MMS-induced mutagenesis level. The various rosehip infusions used did not show statistically significant differences among themselves. The results obtained indicate the antigenotoxic activity of R. majalis infusions.

113 INTERACTIONS BETWEEN THE VISCERAL YOLK SAC PLACENTA AND THE UTERINE WALL IN NECROMYS LASIURUS (RODENTIA, CRICETIDAE)—AN EXPERIMENTAL MODEL TO UNDERSTANDING THE MATERNAL–FETAL EXCHANGE

2012 ◽  
Vol 24 (1) ◽  
pp. 169
Author(s):  
P. O. Favaron ◽  
A. M. Carter ◽  
A. M. Mess ◽  
M. F. Oliveira ◽  
M. A. Miglino
Keyword(s):  
Periodic Acid ◽  
Uterine Wall ◽  
Yolk Sac ◽  
Laboratory Animals ◽  
Nuclear Antigen ◽  
Fetal Membrane ◽  
Strong Response ◽  
Periodic Acid Schiff ◽  
Vital Functions ◽  
Visceral Yolk Sac

The establishment of pregnancy results from the interaction between the trophoblast and maternal tissues. Evolutionarily, the yolk sac (YS) is the only fetal membrane present in all vertebrate taxa. In mammals, it results in the development of the YS placenta, which is responsible for maternal–fetal exchange in the beginning of gestation. Its functions are related to hematopoiesis, selective transfer of various substances including proteins, iron transfer and histiotrophic nutrition. Necromys lasiurus is a rodent species from South America, which is related to species which are used as laboratory animals. Herein, we show data on the early development of the YS to promote a better understanding of its relationship with the uterine wall. Ten implantation sites of Necromys were obtained from a breeding group at the UFERSA, Mossoró, Brazil. Samples were examined by means of histology, immunohistochemistry for vimentin (1:200) and Proliferating Cell Nuclear Antigen (PCNA; 1:800) and scanning and transmission electron microscopy. In early pregnancy the visceral YS was in places closely attached to the uterine wall. At these attachments, the uterus possessed infolded structures with a high-columnar epithelium associated with blood vessels and surrounded by vimentin positive glands. In these areas, the YS became compact in shape and we observed an intimate association of both visceral yolk sac and endometrial luminal epithelium. A strong response to periodic acid Schiff, including plenty of granular vesicles, indicated secretory activity of the endodermal cells, such as release of histiotrophe. Large intercellular spaces, vacuoles and glycogen were present in the cytoplasm of these cells. On the other hand, in areas where the visceral YS was near to the labyrinthine region of chorioallantoic placenta, it was highly villous. The villi were formed by endoderm cells with hexagonal shape. The visceral YS was supplied by fetal vessels, which form the blood islands, structures related to the fetal hematopoiesis. A prominent microvilli area was observed on the surface of the endoderm cells. Additionally, vacuoles, electron-dense inclusions and dense droplets indicative of high secretory and transfer activities were seen inside of these cells. According to PCNA labelling, the mesothelial layer in the region near to uterine consisted of proliferative cells. Although early gestation represents a crucial developmental stage, information about the interaction between the trophoblast and maternal tissues is sparse. Embryonic mortality, a major cause of reproductive failure in livestock species, can be related to interactive problems between maternal and fetal tissues that affect exchange of nutrients, hematopoiesis and other vital functions. Supported by grants from FAPESP (Proc. 09/53392-8).

Effect of combined action of arsenic and antimony compounds on renal function in the subchronic test

2021 ◽  
Author(s):  
N.A. Shestakov ◽  
R.I. Aizman ◽  
A.S. Ogudov ◽  
N.F. Chuenko
Keyword(s):  
Renal Function ◽  
Key Words ◽  
Wistar Rats ◽  
Chemical Elements ◽  
The Body ◽  
Laboratory Animals ◽  
Male Wistar Rats ◽  
Combined Action ◽  
Related Series

The study was conducted on male Wistar rats. We used 4 mathematically related series of doses prepared by sequential dilution of water from a man-made lake according to the scheme: arsenic: 0.15 - 0.05 - 0.016 - 0.0055, antimony: 0.68 - 0.227 - 0.075 - 0.025 mg/l. The animals were examined before inoculation (background), on days 40 and 90 of the experiment, taking into account the processes of cumulation of chemical elements in the kidneys. New data on the nature of nephrotoxic effects of arsenic and antimony in the long-term combined intake into the body of laboratory animals have been obtained. Key words: water from a man-made lake, arsenic and antimony compounds, Wistar rats, nephrotoxicity.

TOXICITY ASSESSMENT OF ENGINEERED NANOPARTICLES USING ALGAE CHLORELLA VULGARIS

2017 ◽  
pp. 50-54
Author(s):  
A. A. Asanova ◽  
V. I. Polonskiy ◽  
Y. S. Grigoriev
Keyword(s):  
Chlorella Vulgaris ◽  
Photosynthetic Activity ◽  
Photosynthetic Apparatus ◽  
Test Organism ◽  
Toxicity Assessment ◽  
Delayed Fluorescence ◽  
Engineered Nanoparticles ◽  
Environmental Objects ◽  
Test Culture ◽  
Potential Risks

The use of nanoparticles covers new spheres of production every year. Their release into the environment is possible at any stage of their lifespan: from the manufacturing process to the utilization in the final product; therefore potential risks they pose for various environmental objects should be evaluated. The purpose of this work was to assess the effect of technogenic nanoparticles on Chlorella vulgaris growth and photosynthetic activity. Toxic properties of three types of nanoparticles TiO2 with a size of 100 to 190 nm, SiO2 with dimensions of 10-15 nm and 100-120 nm have been studied. Chlorella vulgaris Beijer alga was used as test organism. The evaluation of nanoparticles toxicity was based on changes in the relative delayed fluorescence index (RDFI), which characterizes the photosynthetic activity of microalgae. The effect on the growth of the test culture was determined from the optical density (OD) of the algal suspension. It was found that the TiO2 nanoparticles slightly reduced the growth and activity of the cell photosynthetic apparatus. Large particles of SiO2 proved to be more toxic than smaller ones. For nanoparticles of 100-120 nm size , the EC50 value according to the OD index was 8 mg/dm3, while the particles measuring 10-15 nm did not show a significant effect on the growth of the culture. Large SiO2 particles also appeared more toxic than small particles, judging by RDFI parameter.

scholarly journals COMPARATIVE ANALYSIS OF THE INFLUENCE OF COMPLEX MIXTURES OF CHEMICAL AIR POLLUTANTS ON BIOCHEMICAL, PHYSIOLOGICAL, GONADO-, EMBRYOTOXIC INDICES OF LABORATORY ANIMALS AND HYGIENIC EVALUATION OF THEIR COMBINED ACTION

2019 ◽  
Vol 96 (6) ◽  
pp. 528-531
Author(s):  
Malokhat A. Mirzakarimova
Keyword(s):  
Sulfur Dioxide ◽  
Nitrogen Dioxide ◽  
Hydrogen Fluoride ◽  
Lead Acetate ◽  
Ambient Air ◽  
Complex Mixtures ◽  
Laboratory Animals ◽  
Suspended Substances ◽  
Combined Action ◽  
Series Of Experiments

In the paper there are presented results of experimental works on the study of the character of the combined toxic effect of various complex mixtures presenting in atmospheric air. The action of complex mixtures on the organism of laboratory animals was studied in two series of experiments. In the first series of the experiments there was investigated the combined action of sulfur dioxide, nitrogen dioxide, hydrogen fluoride and suspended substances, and in the second series of experiments - of lead acetate, formaldehyde, sulfur dioxide and ammonia.The character of the combined action of lead acetate, formaldehyde, sulfur dioxide, ammonia under one-term inhalation is established to be manifested in the form of “effect-summation”, and the impact of such suspended substances as hydrogen fluoride, sulfur dioxide, nitrogen dioxide was similar to the type of “incomplete summation”. In the joint presence of lead acetate, formaldehyde, sulfur dioxide, ammonia in the ambient air, the maximum permissible concentration (MPC ) for each substance should not exceed 0.25 MPC, and the hydrogen fluoride, nitrogen dioxide, sulfur dioxide and suspended solids should not exceed 0.46 MPC under their isolated action .

The Effect of Stress and Warfarin on the Adrenal Gland in Relation to Spontaneous Hemorrhage, as Judged by Measurement of Adrenal Ascorbic Acid and Serum Corticosterone

1971 ◽  
Vol 26 (02) ◽  
pp. 275-288 ◽  
Author(s):  
S Chattopadhyay ◽  
D. D Johnson ◽  
G. J Millar ◽  
L. B Jaques
Keyword(s):  
Ascorbic Acid ◽  
Acid Concentration ◽  
Ascorbic Acid Concentration ◽  
Corticosterone Concentration ◽  
Serum Corticosterone ◽  
Spontaneous Hemorrhage ◽  
Combined Action ◽  
Series Of Experiments ◽  
Pituitary Adrenal Axis

SummaryRats were subjected to the following procedures: No treatment, Stressor (10% NaCl i.p.), Warfarin for 7 days, Stressor followed by Warfarin; and groups were sacrificed at intervals for assessment of spontaneous hemorrhage and of adrenal ascorbic acid concentration. There was no hemorrhage in the no treatment and stressor groups; some hemorrhage in the warfarin group; profound hemorrhage with Warfarin + Stressor. The adrenal ascorbic acid concentration was found to be lower, 8 h and again 5 days after stress, and remained lower in the warfarin + stress animals. Warfarin had no effect on adrenal ascorbic acid level.In another series of experiments in which the stress consisted of an electric current to the cage floor for 6 sec over 15 min, rats were sacrificed daily for determination of serum corticosterone concentration and occurrence of spontaneous hemorrhage. There was a statistically significant increase of serum corticosterone concentration with stress, warfarin and combined warfarin and stress treatments (P< 0.001 for all three variables). There was a significant correlation (r = 0.96 and 0.89, P< 0.01) for serum corticosterone concentration with hemorrhage score and incidence of hemorrhage in stressed rats receiving warfarin, but not in those receiving only warfarin. The results indicate an activation, rather than an exhaustion, of the pituitary-adrenal axis during the combined action of anticoagulant and stress, which results in the development of spontaneous hemorrhage.

Determination of acute toxicity of Tiacyclin solution for injection in laboratory animals

2019 ◽  
Vol 331 (8) ◽  
pp. 25-27
Author(s):  
N.Y. Morozov ◽  
◽  
S.I. Tchukina ◽  
E.I. Koveshnikova ◽  
◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Laboratory Animals
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