scholarly journals POTENCIAL DE REGENERACIÓN DE Psychotria ipecacuanha (Rubiaceae) A PARTIR DE CAPAS DELGADAS DE CÉLULAS

2015 ◽  
Vol 20 (3) ◽  
pp. 181-192 ◽  
Author(s):  
Catalina BOTERO GIRALDO ◽  
Aura Inés RREA TRUJILLO ◽  
Esther Julia NARANJO GÓMEZ
Keyword(s):  
Growth Regulators ◽  
Somatic Embryos ◽  
Light Condition ◽  
Nodal Explants ◽  
Light Emitting ◽  
Thin Cell Layers ◽  
Cell Layers ◽  
Medicinal Properties ◽  
Leaf Segments

<p>Conociendo las propiedades medicinales de la especie vegetal <em>Psychotria</em> <em>ipecacuanha</em> (Brot.) Stokes, su crítico estado de conservación, así como las dificultades que presenta para la propagación efectiva, el presente estudio tuvo como objetivo evaluar su potencial de propagación por los sistemas de regeneración <em>in vitro</em>, organogénesis y embriogénesis somática. Para este propósito, capas delgadas de células (CDCs) de tallos y de hojas, así como segmentos foliares fueron sometidos a diferentes tratamientos con reguladores de crecimiento y condiciones de luz. Además se estableció el efecto de diferentes longitudes de onda vía diodos emisores de luz (LEDs), sobre la regeneración en estos explantes y nudos provenientes de plantas <em>in vitro</em>. Los resultados obtenidos mostraron que los segmentos de hoja y las CDCs de tallo sembrados en el medio de cultivo MS suplementado con las combinaciones de los reguladores de crecimiento IBA + BAP e IBA + TDZ formaron embriones somáticos y brotes. Los cortes histológicos realizados corroboraron estos dos tipos de origen. Se encontró que bajo la condición lumínica 16/8, se alcanzaron los mejores resultados de inducción de brotes y embriones. En cuanto al efecto de las diferentes longitudes de onda de luz, se encontró que las correspondientes al rojo, verde y blanca, favorecieron el crecimiento y desarrollo de brotes y la inducción de embriones somáticos. El desarrollo de los brotes a partir de los nudos no presentó diferencias estadísticas entre los tratamientos con LEDs, por lo que se recomienda el uso de la luz blanca continua y con fotoperiodo durante el proceso de multiplicación y desarrollo de estos.</p><p><strong>Regeneration Potential of <em>Psychotria Ipecacuanha</em> (Rubiaceae) from Thin Cell Layers </strong></p><p>Knowing the medicinal properties of the plant specie <em>Psychotria ipecacuanha</em> (Brot.) Stokes, its critical condition and the difficulties for its effective propagation, the present study aimed to assess the potential of propagation of <em>P. ipecacuanha</em> by <em>in vitro</em> systems of regeneration, organogenesis and somatic embryogenesis. For this purpose, thin cell layers (TCL) of stems and leaves, as well as leaf segments were subjected to different treatments of plant growth regulators and light conditions. Furthermore, the effect of different wavelengths via light emitting diodes (LED’s), was established for the regeneration in these explants and nodal explants from <em>in vitro</em> plants. The results showed that leaf segments and stem TCL, cultured in MS medium supplemented with the combinations of growth regulators IBA + BAP and IBA + TDZ, formed somatic embryos and shoots. The histological sections supported the two types of source. It was found that the best results in shoot induction and embryos were achieved under the light condition 16/8-h light/dark. Regarding the effect of different wavelengths, it was found that those corresponding to red, green, and white supported the growth and shoot development as well as somatic embryos induction. The shoots development from the nodal explants did not show statistical differences between LEDs treatments, so the use of a continuous white light and photoperiod is recommended during their multiplication and development.</p>

Somatic Embryogenesis and In vitro Plant Regeneration in Vigna trilobata (L.) Verd. - A Potential Pasture Legume

1970 ◽  
Vol 19 (1) ◽  
pp. 89-99
Author(s):  
K. Choudhary ◽  
M. Singh ◽  
M. S. Rathore ◽  
N. S. Shekhawat
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Long Term Study ◽  
Continuous Application ◽  
First Time ◽  
Pasture Legume

This long term study demonstrates for the first time that it is possible to propagate embryogenic Vigna trilobata and to subsequently initiate the differentiation of embryos into complete plantlets. Initiation of callus was possible on 2,4-D. Somatic embryos differentiated on modified MS basal nutrient medium with 1.0 mg/l  of 2,4-D and 0.5 mg/l  of Kn. Sustained cell division resulted in globular and heart shape stages of somatic embryos. Transfer of embryos on to a fresh modified MS basal medium with 0.5 mg/l of Kn and 0.5 mg/l of GA3 helped them to attain maturation and germination. However, the propagation of cells, as well as the differentiation of embryos, were inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking these growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation of embryogenic cultures in Vigna species are discussed. Key words: Pasture  legume, Vigna trilobata, Globular, Heart shape, somatic embryogenesis D.O.I. 10.3329/ptcb.v19i1.4990 Plant Tissue Cult. & Biotech. 19(1): 89-99, 2009 (June)

scholarly journals Assessment of In-vitro culture through Nodal explants of Dendrocalamus hamiltonii

2021 ◽  
Vol 2 (1) ◽  
pp. 130-133
Author(s):  
Abha Jha ◽  
◽  
Sunila Das ◽  
Keyword(s):  
Experimental Study ◽  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Shoot Multiplication ◽  
Growth Hormones ◽  
Nodal Explants ◽  
Bud Break ◽  
Multiplication Rate ◽  
Dendrocalamus Hamiltonii

The present experimental study was aimed to overcome the traditional methods of propagation that limit the number of propagules by in-vitro regeneration through nodal explants of Dendrocalamus hamiltonii with a comparative study of growth regulators during the shooting and rooting process. Dendrocalamus hamiltonii is distributed from the Himalayas (Nepal) to the northern part of Burma. Collection of explants was done from different selected sites of CPTs. There was the use of HgCl2 and Ca (OCl)2 as sterilizing agents in different concentrations and its effect was visualized during the sprouting stage. Culm explants were cultured in a bottle containing White media (Wm) supplemented with BA and Kinetin for sprouting and IAA, IBA, NAA for rooting. There is also the use of IAA+IBA+NAA in combined form as a supplementary solution 0.1% HgCl2 treatment for 20-minute results into77.80% aseptic buds and 72% bud -break. Among the used growth-hormones, BA with concentration 0.25mg/l and 0.50mg/l respectively were appropriate for shoot-multiplication rate, 4.01±0.3 and 4.3±0.4 were ideal observation incorporation with BA (1.00mg/l) and BA (1.50mg/l) respectively. Maximum sprouting rate14.77±3.37with application of BA (2.00mg/l) and maximum shoot length4.3±0.4 is observed at BA (1.50mg/l). The applications of rooting hormone IAA+IBA+NAA in the concentration of 1.0 mg/l results in 72.5±0.3(rooting) and 11.1±0.3 (av. No. of the root).

scholarly journals In vitro ROOTING OF TENERA HYBRID OIL PALM (Elaeis guineensis Jacq.) PLANTS1

2018 ◽  
Vol 41 (4) ◽  
Author(s):  
Marlúcia Souza Pádua ◽  
Raíssa Silveira Santos ◽  
Luciano Vilela Paiva ◽  
Vanessa Cristina Stein ◽  
Luciano Coutinho Silva
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Oil Palm ◽  
Somatic Embryos ◽  
Large Scale ◽  
Elaeis Guineensis ◽  
Germination Percentage ◽  
Shoot Length ◽  
Indole Butyric Acid

ABSTRACT Oil palm is a woody monocot of economic importance due to high oil production from its fruits. Currently, the conventional method most used to propagate oil palm is seed germination, but success is limited by long time requirements and low germination percentage. An alternative for large-scale propagation of oil palm is the biotechnological technique of somatic embryogenesis. The rooting of plants germinated from somatic embryos is a difficult step, yet it is of great importance for later acclimatization and success in propagation. The aim of this study was to evaluate the effect of the auxins indole acetic acid (IAA) and indole butyric acid (IBA) on the rooting of somatic embryos of Tenera hybrid oil palm. Plants obtained by somatic embryogenesis were inoculated in modified MS medium with 10% sucrose and 0.6% agar and supplemented with IAA or IBA at concentrations of 5 µM, 10 µM, and 15 µM, and the absence of growth regulators. After 120 days, the presence of roots, root type, length of the longest root, number of roots, number of leaves, and shoot length were analyzed. Growth regulators were favorable to rooting; plants cultivated with IBA growth regulator at 15 µM showed higher rooting percentage (87%) and better results for the parameters of number of roots (1.33) and shoot length (9.83).

Morphological and physiological characteristics of rapeseed plants regenerated in vitro from thin cell layers in the presence of zinc

2007 ◽  
Vol 330 (10) ◽  
pp. 728-734 ◽  
Author(s):  
Asma Ben Ghnaya ◽  
Gilbert Charles ◽  
Annick Hourmant ◽  
Jeannette Ben Hamida ◽  
Michel Branchard
Keyword(s):  
Physiological Characteristics ◽  
Thin Cell Layers ◽  
Cell Layers

scholarly journals 331 Effects of Explant Type, Sucrose Level and Callus Development Time on In Vitro Plant Regeneration of Sweetpotato

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 449A-449
Author(s):  
L. Chen ◽  
S.O. Park ◽  
S. Dhir ◽  
A.S. Bhagsari
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Development Time ◽  
Shoot Tip ◽  
Explant Type ◽  
Callus Initiation ◽  
Sucrose Level

Several experiments were conducted to evaluate the influence of explant type, sucrose level, and callus development time on sweetpotato [Ipomoea batatas (L.) Lam] in vitro culture. Shoot tip, petiole, and leaf of Selection 75-96-1 was used as explants in Murashige and Skoog (MS) media with different plant growth regulators. Calli derived from shoot tip and petiole produced 42.1% and 10.3% somatic embryos, respectively, but the leaf failed to produce somatic embryos. The effect of sucrose level was determined using shoot tip as explants. Compared with 3% sucrose in the same plant growth regulators level medium during callus initiation and callus proliferation periods, 5% sucrose level suppressed root growth and improved shoot regeneration. The callus development time was measured by using shoot tips on callus initiation medium containing 1.5 mg/L alpha-Naphthaleneacetic acid (NAA) and 0.25 mg/L Kinetin (KIN) plus 5% sucrose. When explants were cultured for less than 6 weeks during callus initiation, then transferred onto plant regeneration medium, plant regeneration via organogenesis occurred; whereas, maintaining cultures for more than 12 weeks on the same callus initiation/proliferation medium, plant regeneration was favored via embryogenesis. Explant type and other factors affecting plant regeneration noted here could be applied to protoplast culture, somatic hybridization, and transformation in sweetpotato.

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