scholarly journals Immobilization of DNA probes on a high frequency piezoelectric biosensor

DYNA ◽  
2020 ◽  
Vol 87 (212) ◽  
pp. 163-168
Author(s):  
Camilo Ortiz Monsalve ◽  
Jorge Mario Guerra González ◽  
Marisol Jaramillo Grajales
Keyword(s):  
Real Time ◽  
High Frequency ◽  
Quartz Crystal ◽  
Low Cost ◽  
Fast Response ◽  
Dna Probes ◽  
Self Assembled Monolayers ◽  
Saturation Point ◽  
Assembled Monolayers ◽  
Quartz Crystal Microbalances

In recent years, researchers have taken to biosensors as effective tools for detection due to their portability, low-cost, fast response, and practicality. Piezoelectricity gave way to quartz crystal microbalances (QCM), of which high-frequency QCMs (HFF-QCM 100MHz) are still being researched. In this paper, we use DNA immobilization on a HFF-QCM via self-assembled monolayers (SAM) technique. Immobilization was initially verified with ATR-FTIR. Then, DNA was immobilized in real time on the HFF-QCM crystals. A variation in the phase of the signal suggests fixation of DNA to the surface, in accordance with ATR-FTIR results. A density of 629 ng/cm2 was computed. Also, a positive correlation between immobilized DNA and DNA concentration, and the appearance of a saturation point between 1 and 5 μM were shown after analysis of different DNA concentrations.

scholarly journals Development of a high frequency piezoelectric immunosensor for the detection and quantification of BSA

2020 ◽  
Vol 10 (3) ◽  
pp. 5400-5405
Keyword(s):  
High Frequency ◽  
Quartz Crystal ◽  
Limit Of Detection ◽  
Low Frequency ◽  
Coefficient Of Determination ◽  
Self Assembled Monolayers ◽  
Binding Behavior ◽  
Piezoelectric Immunosensor ◽  
Assembled Monolayers ◽  
The Hill

In this work, we developed a High Fundamental Frequency –Quartz Crystal Microbalance (HFF-QCM) immunosensor for the quantification of Bovine Serum Albumin (BSA) protein. Immobilization of BSA was achieved by means of mixed self-assembled monolayers (SAM), and we found that the largest phase shifts were produced with a concentration of 10 mg/mL. Then, we plotted an anti-BSA vs phase shift calibration curve, and obtained the analytical parameters that allowed us to compare our sensor to another anti–BSA QCM-based sensors. Our HFF-QCM immunosensor displays more sensitivity than low frequency QCM-based biosensors: its limit of detection (LOD) is 100 ng/mL and its linear range extends from 100 ng/mL to 5000 ng/mL. Finally, we fitted different adsorption isotherms models to our experimental data. We chose the Hill isotherm as it presented the highest coefficient of determination (R2), and we determined that the interaction between BSA and anti-BSA displays a positively cooperative binding behavior.

scholarly journals A Real-Time Method for Improving Stability of Monolithic Quartz Crystal Microbalance Operating under Harsh Environmental Conditions

Sensors ◽  
2021 ◽  
Vol 21 (12) ◽  
pp. 4166
Author(s):  
Román Fernández ◽  
María Calero ◽  
Yolanda Jiménez ◽  
Antonio Arnau
Keyword(s):  
Real Time ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Limit Of Detection ◽  
Quartz Substrate ◽  
Low Cost ◽  
Discrete Wavelet ◽  
Reagent Consumption ◽  
Measurement Cell ◽  
The Stability

Monolithic quartz crystal microbalance (MQCM) has recently emerged as a very promising technology suitable for biosensing applications. These devices consist of an array of miniaturized QCM sensors integrated within the same quartz substrate capable of detecting multiple target analytes simultaneously. Their relevant benefits include high throughput, low cost per sensor unit, low sample/reagent consumption and fast sensing response. Despite the great potential of MQCM, unwanted environmental factors (e.g., temperature, humidity, vibrations, or pressure) and perturbations intrinsic to the sensor setup (e.g., mechanical stress exerted by the measurement cell or electronic noise of the characterization system) can affect sensor stability, masking the signal of interest and degrading the limit of detection (LoD). Here, we present a method based on the discrete wavelet transform (DWT) to improve the stability of the resonance frequency and dissipation signals in real time. The method takes advantage of the similarity among the noise patterns of the resonators integrated in an MQCM device to mitigate disturbing factors that impact on sensor response. Performance of the method is validated by studying the adsorption of proteins (neutravidin and biotinylated albumin) under external controlled factors (temperature and pressure/flow rate) that simulate unwanted disturbances.

“Vanishing mass” in the Sauerbrey world: quartz crystal microbalance study of self-assembled monolayers based on a tripod-branched structure with tuneable molecular flexibility

The Analyst ◽  
2020 ◽  
Vol 145 (2) ◽  
pp. 656-666 ◽  
Author(s):  
Sergii Kravchenko ◽  
Boris Snopok
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Self Assembled Monolayers ◽  
Sensitive Layer ◽  
Spatial Organisation ◽  
Molecular Flexibility ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Branched Structure

Complex loadings that appear on a surface with flexible spatial organisation can reveal anti-Sauerbrey behaviour due to their variable interfacial architecture even for an ultrathin monomolecular sensitive layer.

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