scholarly journals Flax-seed oil and Lactobacillus plantarum supplementation modulate TLR and NF-κB gene expression in enterotoxigenic Escherichia coli challenged gnotobiotic pigs

2014 ◽  
Vol 62 (4) ◽  
pp. 463-472 ◽  
Author(s):  
Mária Chytilová ◽  
Radomíra Nemcová ◽  
Soňa Gancarčíková ◽  
Dagmar Mudroňová ◽  
Ľudmila Tkáčiková
Keyword(s):  
Escherichia Coli ◽  
Lactobacillus Plantarum ◽  
Seed Oil ◽  
Probiotic Strain ◽  
Nuclear Factor Κb ◽  
Enterotoxigenic Escherichia Coli ◽  
Flax Seed ◽  
Natural Agents ◽  
Gnotobiotic Pigs ◽  
Probiotic Lactobacilli

The present study analyses the effect of flax-seed oil rich in n-3 polyunsaturated fatty acids (PUFAs), the probiotic strain Lactobacillus plantarum — Biocenol™ LP96 and their combination on the expression level of selected Toll-like receptor (TLR) genes (TLR2, TLR4, TLR5, TLR9) and their downstream molecules (myeloid differentiation factor 88, MyD88; nuclear factor-κB, NF-κB) in the jejunum of gnotobiotic pigs challenged with enterotoxigenic Escherichia coli (ETEC). The results show that both immunomodulators are able to modulate the RNA level of at least one of the target molecules and thus regulate pathogeninduced inflammation. We confirmed that not only probiotic lactobacilli or flaxseed oil alone but also their synergistic action has great potential in the prevention and treatment of porcine colibacillosis. The results give an insight into one of the possible mechanisms by which natural agents, such as probiotic lactobacilli and flax-seed oil, exert their immunoregulatory properties during pathogen-induced inflammation.

The effect of supplementation of flax-seed oil on interaction of Lactobacillus plantarum – Biocenol™ LP96 and Escherichia coli O8:K88ab:H9 in the gut of germ-free piglets

2012 ◽  
Vol 93 (1) ◽  
pp. 39-41 ◽  
Author(s):  
Radomíra Nemcová ◽  
Dana Borovská ◽  
Jana Koščová ◽  
Soňa Gancarčíková ◽  
Dagmar Mudroňová ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactobacillus Plantarum ◽  
Seed Oil ◽  
Flax Seed ◽  
Germ Free

Alleviation of enterotoxigenic Escherichia coli challenge by recombinant Lactobacillus plantarum expressing a FaeG- and DC-targeting peptide fusion protein

2017 ◽  
Vol 8 (3) ◽  
pp. 379-391 ◽  
Author(s):  
G. Yang ◽  
Y. Jiang ◽  
P. Tong ◽  
C. Li ◽  
W. Yang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactobacillus Plantarum ◽  
Body Weight Gain ◽  
Immunoglobulin A ◽  
Enterotoxigenic Escherichia Coli ◽  
Oral Vaccination ◽  
Beneficial Effects ◽  
Targeting Peptide ◽  
Spleen And Lymph Nodes ◽  
K88 Fimbriae

FaeG is the major subunit of K88 fimbriae. These cell surface attachments are considered to be the major virulence factor of enterotoxigenic Escherichia coli (ETEC), which causes diarrhoea in piglets. The use of dendritic cell-targeting peptide (DCpep) has been demonstrated to be an effective approach to enhance the immunity of vaccines. Lactobacillus plantarum is an attractive candidate for oral vaccination owing to its beneficial effects and safety. In this study, L. plantarum was employed to deliver a FaeG-DCpep fusion antigen, and the immune response in mice was evaluated. The synthesis of FaeG-DCpep dramatically increased the adhesion of recombinant L. plantarum (RLP) to IPEC-J2 cell surfaces, resulting in direct competition between L. plantarum and ETEC during adhesion assays. Significantly higher levels of body weight gain, sera immunoglobulin G and intestinal immunoglobulin A were observed in BALB/c mice immunised with RLP. In addition, the number of CD19+ B cells and CD11c+DC cells and the expression levels of several cytokines in the spleen and lymph nodes increased significantly compared to non-immunised mice. The oral administration of RLP also alleviated the symptoms of ETEC challenge, as shown by haematoxylin-eosin staining, indicating that RLP may be an efficient vaccine candidate.

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