Alleviation of enterotoxigenic Escherichia coli challenge by recombinant Lactobacillus plantarum expressing a FaeG- and DC-targeting peptide fusion protein

2017 ◽  
Vol 8 (3) ◽  
pp. 379-391 ◽  
Author(s):  
G. Yang ◽  
Y. Jiang ◽  
P. Tong ◽  
C. Li ◽  
W. Yang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactobacillus Plantarum ◽  
Body Weight Gain ◽  
Immunoglobulin A ◽  
Enterotoxigenic Escherichia Coli ◽  
Oral Vaccination ◽  
Beneficial Effects ◽  
Targeting Peptide ◽  
Spleen And Lymph Nodes ◽  
K88 Fimbriae

FaeG is the major subunit of K88 fimbriae. These cell surface attachments are considered to be the major virulence factor of enterotoxigenic Escherichia coli (ETEC), which causes diarrhoea in piglets. The use of dendritic cell-targeting peptide (DCpep) has been demonstrated to be an effective approach to enhance the immunity of vaccines. Lactobacillus plantarum is an attractive candidate for oral vaccination owing to its beneficial effects and safety. In this study, L. plantarum was employed to deliver a FaeG-DCpep fusion antigen, and the immune response in mice was evaluated. The synthesis of FaeG-DCpep dramatically increased the adhesion of recombinant L. plantarum (RLP) to IPEC-J2 cell surfaces, resulting in direct competition between L. plantarum and ETEC during adhesion assays. Significantly higher levels of body weight gain, sera immunoglobulin G and intestinal immunoglobulin A were observed in BALB/c mice immunised with RLP. In addition, the number of CD19+ B cells and CD11c+DC cells and the expression levels of several cytokines in the spleen and lymph nodes increased significantly compared to non-immunised mice. The oral administration of RLP also alleviated the symptoms of ETEC challenge, as shown by haematoxylin-eosin staining, indicating that RLP may be an efficient vaccine candidate.

Gastrointestinal ecology response of piglets’ diets containing non-starch polysaccharide hydrolysis products and egg yolk antibodies following an oral challenge with Escherichia coli (k88)

2009 ◽  
Vol 89 (3) ◽  
pp. 341-352 ◽  
Author(s):  
E Kiarie ◽  
B A Slominski ◽  
D O Krause ◽  
C M Nyachoti
Keyword(s):  
Escherichia Coli ◽  
Egg Yolk ◽  
Intestinal Morphology ◽  
Oral Challenge ◽  
Enterotoxigenic Escherichia Coli ◽  
Adaptation Period ◽  
Egg Yolk Antibodies ◽  
Hydrolysis Products ◽  
K88 Fimbriae ◽  
Polysaccharide Hydrolysis

The gastrointestinal ecology (GE) of piglets fed diets containing non-starch polysaccharide hydrolysis products (HP) and egg yolk antibodies against K88 fimbriae (EYA) following oral challenge with enterotoxigenic Escherichia coli K88 (ETEC) was investigated. The HP were products of incubating feedstuffs with a blend of carbohydrase enzymes. Forty, 21-d-old pigs (two pigs/pen) were assigned to four diets to give five pens per diet. The diets were: a control fed without or with 5 g kg-1 of HP and EYA either singly or in combination forming a 2 × 2 factorial arrangement. Following a 9-d adaptation period, all pigs were orally challenged with ETEC and killed at 24 and 48 h post-challenge (one pig/pen on each occasion). Feeding HP increased pre-challenge average daily gain (252 vs. 207 g d-1; P = 0.01). An interaction (P < 0.10) between EYA and HP was observed such that when fed in combination they resulted in higher ileal digesta lactic acid and cecal DM contents and lower ileal digesta ammonia. The main effects (P < 0.05) were such that pigs fed EYA-diets had shorter intestinal crypt whilst pigs fed HP-diets showed low gastric pH and high ileal mucosal adherent lactobacilli counts. In conclusion, HP and EYA influenced indices of fermentative characteristics and intestinal morphology in the gastrointestinal ecology of piglets orally challenged with enterotoxigenic E. coli (k88).Key words: Egg yolk antibodies, ETEC, gastrointestinal ecology, non-starch polysaccharides hydrolysis products, piglet

scholarly journals Intestinal Immunoglobulin A Response to Naturally Acquired Enterotoxigenic Escherichia coli in US Travelers to an Endemic Area of Mexico

2006 ◽  
Vol 9 (5) ◽  
pp. 247-250 ◽  
Author(s):  
M. T. Estrada-García ◽  
Z-D. Jiang ◽  
J. Adachi ◽  
J. J. Mathewson ◽  
H. L. DuPont
Keyword(s):  
Escherichia Coli ◽  
Endemic Area ◽  
Immunoglobulin A ◽  
Enterotoxigenic Escherichia Coli

scholarly journals PSVII-16 Galactosylated chitosan-oligosaccharides have anti-adhesive effect against enterotoxigenic Escherichia coli in piglets

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 224-224
Author(s):  
Charlotte Maria Elisabeth Heyer ◽  
Weilan Wang ◽  
Yalu Yan ◽  
Michael G Gänzle ◽  
Ruurd T Zijlstra
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Rrna Genes ◽  
Saline Control ◽  
Fluid Loss ◽  
Bacterial Gene ◽  
Test Product ◽  
Chitosan Oligosaccharides ◽  
Galactosylated Chitosan ◽  
K88 Fimbriae

Abstract Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea in piglets. In vitro, high molecular weight β-galactosylated chitosan-oligosaccharides (Gal-COS) had strong anti-adhesive activity against ETEC-expressing K88 fimbriae (ETEC K88) binding to porcine erythrocytes. This study assessed the effects of Gal-COS differing in structure on anti-adhesive properties against ETEC in a small intestinal segment perfusion (SISP) model in 8 piglets (BW 10 kg; 5-wk old). With 10 jejunal segments in each pig, 5 segments were infected with ETEC K88, and the other 5 segments were infused with saline (non-ETEC). Every 2 paired segments (ETEC or non-ETEC) from the same pig were treated for 8 h with 64 ml of 10 g L-1 of one of the following test products: 1) α-Gal-COS; 2) β-Gal-COS; 3) exopolysaccharides produced by Lactobacillus reuteri; and 4) raffinose in a double 4 × 4 Latin square with a saline control. Infection by ETEC K88 was verified by quantitative PCR. Net fluid loss was calculated as difference of fluid loss between ETEC segment and its paired non-ETEC segment. Data were analyzed using the mixed model with segment and test product as fixed effects, and pig as random effect. Number of eubacterial rRNA genes was 10-fold greater (P < 0.001) in ETEC segments than non-ETEC segments, indicating that ETEC K88 accounted for > 90% of bacterial gene counts. Test product did not affect (P > 0.10) the number of ETEC bacteria in the outflow fluid. Furthermore, net fluid loss caused by ETEC tended (P = 0.08) to be decreased by β-Gal-COS compared to all other treatments. In conclusion, the in vivo SISP model confirmed that Gal-COS had anti-diarrheal effects, indicating that β-Gal-COS is a potential feed additive to reduce the ETEC-induced diarrhea in piglets.

Adjuvant effect of Gantrez®AN nanoparticles during oral vaccination of piglets against F4+enterotoxigenic Escherichia coli

2011 ◽  
Vol 139 (2-4) ◽  
pp. 148-155 ◽  
Author(s):  
Katrien Vandamme ◽  
Melkebeek Vesna ◽  
Cox Eric ◽  
Remon Jean Paul ◽  
Vervaet Chris
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Adjuvant Effect ◽  
Oral Vaccination

scholarly journals Flax-seed oil and Lactobacillus plantarum supplementation modulate TLR and NF-κB gene expression in enterotoxigenic Escherichia coli challenged gnotobiotic pigs

2014 ◽  
Vol 62 (4) ◽  
pp. 463-472 ◽  
Author(s):  
Mária Chytilová ◽  
Radomíra Nemcová ◽  
Soňa Gancarčíková ◽  
Dagmar Mudroňová ◽  
Ľudmila Tkáčiková
Keyword(s):  
Escherichia Coli ◽  
Lactobacillus Plantarum ◽  
Seed Oil ◽  
Probiotic Strain ◽  
Nuclear Factor Κb ◽  
Enterotoxigenic Escherichia Coli ◽  
Flax Seed ◽  
Natural Agents ◽  
Gnotobiotic Pigs ◽  
Probiotic Lactobacilli

The present study analyses the effect of flax-seed oil rich in n-3 polyunsaturated fatty acids (PUFAs), the probiotic strain Lactobacillus plantarum — Biocenol™ LP96 and their combination on the expression level of selected Toll-like receptor (TLR) genes (TLR2, TLR4, TLR5, TLR9) and their downstream molecules (myeloid differentiation factor 88, MyD88; nuclear factor-κB, NF-κB) in the jejunum of gnotobiotic pigs challenged with enterotoxigenic Escherichia coli (ETEC). The results show that both immunomodulators are able to modulate the RNA level of at least one of the target molecules and thus regulate pathogeninduced inflammation. We confirmed that not only probiotic lactobacilli or flaxseed oil alone but also their synergistic action has great potential in the prevention and treatment of porcine colibacillosis. The results give an insight into one of the possible mechanisms by which natural agents, such as probiotic lactobacilli and flax-seed oil, exert their immunoregulatory properties during pathogen-induced inflammation.

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