scholarly journals PREVALENCE OF BOVINE HERPESVIRUS 1 (BHV-1) INFECTION IN HUNGARIAN CATTLE HERDS

1999 ◽  
Vol 47 (3) ◽  
pp. 303-309 ◽  
Author(s):  
L. Tekes ◽  
B. Markos ◽  
J. Méhesfalvi ◽  
Zsuzsanna Máté ◽  
E. Kudron ◽  
...  
Keyword(s):  
Large Scale ◽  
Close Contact ◽  
Bovine Herpesvirus ◽  
Small Scale ◽  
Serum Samples ◽  
Bovine Herpesvirus 1 ◽  
Holstein Friesian ◽  
Herpesvirus 1 ◽  
Cattle Herds ◽  
Large Farms

Hungarian cattle herds were surveyed for bovine herpesvirus 1 (BHV-1) infection by ELISA of milk and serum samples. In 1993, 75% of the large cattle herds (consisting of more than 50 cattle) and all small herds (small-scale producers' stocks), while in 1997 90% of the small herds were included in the survey. In the case of large herds, 79.3% of the herds and 64.1% of the samples tested were found to be positive. Of the small herds, 13.5% and 15.7% tested positive in 1993 and 1997, respectively. The majority of large herds were Holstein-Friesian dairy stocks. Small herds with an infection rate markedly exceeding the average were found in those counties where the small herds had been in close contact with the large-scale farms, or where new herds were established by using animals of uncontrolled infectious bovine rhinotracheitis (IBR) status originating from large farms. Attention is called to the importance of maintaining the IBR-free status of small herds that constitute one-third of the Hungarian cattle population.

scholarly journals Antibodies against Bovine herpesvirus 1 in dairy herds in the state of Espirito Santo, Brasil

Revista CERES ◽  
2014 ◽  
Vol 61 (2) ◽  
pp. 280-283 ◽  
Author(s):  
Marcus Rebouças Santos ◽  
Hanna Carolina Campos Ferreira ◽  
Marcos Antônio dos Santos ◽  
Giuliana Loreto Saraiva ◽  
Natália Filardi Tafuri ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Neutralizing Antibodies ◽  
Bovine Herpesvirus ◽  
The State ◽  
Serum Samples ◽  
Bovine Herpesvirus 1 ◽  
Espírito Santo ◽  
Herpesvirus 1 ◽  
Cattle Herds ◽  
Espirito Santo

Bovine herpesvirus 1 (BoHV-1) causes major losses in worldwide livestock, affecting the respiratory and reproductive tracts of bovine. In the past decades, the number of cases in Brazil has been gradually increasing. Therefore, it is important to assess the distribution of infection in different regions of the country. In the state of Espírito Santo (ES) the BoHV 1 infection rate in dairy cattle herds is unknown. Thus, the aim of this study was to detect neutralizing antibodies against BoHV-1 in serum samples from 1,161 non-vaccinated cows from 59 dairy cattle herds in 23 municipalities of the Metropolitan, North, Northwest and South macro-regions. The identification of seropositive cows was evaluated by the virus neutralization test. The results showed that of all serum samples evaluated 775 (66.75%) had neutralizing antibodies against BoHV-1. Moreover, all herds were found positive; however, the percentage of positive cows varied among regions; 49.06%, 62.15%, 67.21% and 80.04% for the Metropolitan, South, North and Northwest macro-regions, respectively. In this study, the results clearly indicate the dissemination of the viral agent in dairy cattle in the ES state, requiring the monitoring and control of diseases related to BoHV-1 infection.

scholarly journals Detection of Bovine Herpesvirus-1-Specific IgM Using a Capture Enzyme Immune Assay with Isotype-Specific Monoclonal Antibodies

1989 ◽  
Vol 1 (2) ◽  
pp. 139-145 ◽  
Author(s):  
Fernando Osorio ◽  
Subramaniam Srikumaran ◽  
Marvin Rhodes ◽  
David Christensen ◽  
Pushpa Srikumaran
Keyword(s):  
Monoclonal Antibodies ◽  
Solid Phase ◽  
Bovine Herpesvirus ◽  
Immunoglobulin M ◽  
Viral Reactivation ◽  
Clinical Samples ◽  
Serum Samples ◽  
Bovine Herpesvirus 1 ◽  
Igm Antibody ◽  
Herpesvirus 1

The detection of virus-specific immunoglobulin M (IgM) antibodies in acute-phase serum samples offers the possibility of making an accurate and rapid serologic diagnosis. We have developed a solid-phase capture assay that uses murine monoclonal antibodies specific for bovine IgM to separate the whole IgM fraction of a bovine serum sample. The IgM specific for bovine herpesvirus-1 (BHV-1) is then detected by the addition of viral antigen, which in turn is detected by BHV-1-specific monoclonal antibodies conjugated to horseradish peroxidase. A BHV-1 IgM antibody response was detected during the early postinfection period (7–40 days PI). Bovine herpesvirus-1 IgM antibody was not detected in sera taken from 3 animals following dexamethasone-induced viral reactivation. This method compares favorably with viral isolation, antigen detection in the clinical samples, and paired serology in the diagnosis of BHV-1 infection at a herd level.

scholarly journals Establishing presence of antibodies against bovine respiratory syncytial virus (BRSV), parainfluenza virus 3 (PI3) and bovine herpesvirus 1 (BHV 1) in blood serum of cattle using indirect immunoenzyme probe

2009 ◽  
Vol 63 (3-4) ◽  
pp. 145-152 ◽  
Author(s):  
Horea Samanc ◽  
Nenad Milic ◽  
Velibor Stojic ◽  
Dejan Knezevic ◽  
Ivan Vujanac ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Blood Serum ◽  
Bovine Herpesvirus ◽  
Bovine Respiratory Syncytial Virus ◽  
Parainfluenza Virus ◽  
Serum Samples ◽  
Bovine Herpesvirus 1 ◽  
Specific Antibodies ◽  
Herpesvirus 1 ◽  
Syncytial Virus

A total of 92 samples of bovine blood serum were examined for the presence of antibodies against the bovine respiratory syncytial virus using indirect immunoenzyme probe - iELISA. Specific antibodies against the bovine respiratory syncytial virus (BRSV) were established in 46, or 50% blood serum samples. Investigations of the 92 blood serum samples of cattle for the presence of antibodies against the parainfluenza virus 3 (PI 3), revealed their presence in 77, or 83.69% of the samples, and the presence of antibodies against the bovine herpesvirus 1 (BHV 1) was established in 19, or 20.65% of the samples.

scholarly journals Investigations of presence of antibodies against bovine herpesvirus-1 in blood serum of calves prior to colostrum diet

2010 ◽  
Vol 64 (1-2) ◽  
pp. 33-41
Author(s):  
Sava Lazic ◽  
Dragan Rogan ◽  
Tamas Petrovic ◽  
Dejan Bugarski ◽  
Diana Lupulovic ◽  
...  
Keyword(s):  
Blood Serum ◽  
Antibody Titer ◽  
Bovine Herpesvirus ◽  
Virus Neutralization ◽  
Serum Samples ◽  
Bovine Herpesvirus 1 ◽  
Breeding Material ◽  
Mdbk Cells ◽  
Herpesvirus 1

The paper presents the results of investigations of the presence of the bovine herpesvirus-1 (BHV-1) in samples of blood serum from 106 cows and 107 of their calves (one cow had twins). Blood was sampled from the cows immediately after parturition, and from the calves before feeding on colostrum. The examined cows and their calves originated from 5 herds in which previous investigations had shown infection with the bovine herpesvirus-1. The determination of antibodies against BHV-1 was performed using the method of virus neutralization in culture of MDBK cells with 100 TCID/50 viruses (BHV-1, TN-41 Am. Bio Research, USA). Antibodies against BHV-1 were determined in all blood serum samples of cows and in 16 samples of precolostral blood serums of calves. The antibody titer values in cows ranged from 1:4 to 1:512, and in calves the determined values were from 1:2 to 1:16. The results indicate that cows that are seropositive to BHV-1 can deliver calves seropositive to BHV-1 in about 15% cases. This must be kept in mind in selecting cows for the production of breeding material, in particular bulls for reproduction centers, as well as in making a programme for the immunoprophylaxis of calves against BHV-1. .

Seroprevalence and Risk Factors of Bovine Herpesvirus 1 Infection in Cattle Herds in the State of Paraná, Brazil

2012 ◽  
Vol 60 (1) ◽  
pp. 39-47 ◽  
Author(s):  
J. A. Dias ◽  
A. A. Alfieri ◽  
J. S. Ferreira-Neto ◽  
V. S. P. Gonçalves ◽  
E. E. Muller
Keyword(s):  
Risk Factors ◽  
Bovine Herpesvirus ◽  
The State ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1 ◽  
Cattle Herds

Evidences of Serological Studies for The Presence of Infectious Bovine Rhinotracheitis IBR, In Albania

1970 ◽  
Vol 3 (1) ◽  
pp. 16-21
Author(s):  
Arta Lugaj ◽  
Liljana Cara ◽  
Marsel Borakaj ◽  
Kristaq Bërxholi
Keyword(s):  
Direct Contact ◽  
Indirect Elisa ◽  
Bovine Herpesvirus ◽  
Infectious Bovine Rhinotracheitis ◽  
Serum Samples ◽  
Chi Square ◽  
Bovine Herpesvirus 1 ◽  
Viral Pathogen ◽  
Herpesvirus 1 ◽  
First Time

Bovine herpesvirus 1 (BHV-1), the causative agent of infectious bovine rhinotracheitis (IBR), is considered to be the most common viral pathogen found in bovine. Virus enters through aerosol route or by direct contact with the nasal secretion in case of respiratory tract infection and by direct contact or by semen containing virus in case of genital infection. For the first time in Albania, this study was conducted to know the status of bovine herpesvirus-1 (BHV-1) antibodies in the bovines of the selected area of Albania. Antibody level was measured using a commercial indirect ELISA. A total of 263 collected serum samples from 7 areas of Albania were subjected to serum neutralization test for detection of BHV-1 antibodies by using of Indirect-ELISA kits. The chi-square test was used for comparison of results between regions and in this study p Values>0.01 was considered statistically no-significant at the 0.01 level. From these results we had an indication about the antibody prevalence of IBR infection respectively, 96% in Terpan-Berat, 52% in Fejza-Has, 50% in Kavajë, 33% in Rrëshen, 14.3% in Guras-Pogradec, 10% in Drenovë-Korçë and 0% in Fier. The prevalence was ranged from 10% to 96% among seropositive herds in this study. In conclusion, results of this study clearly established for the first time that BHV-1 is subclinical prevalent virus in bovine in Albania. Further studies are needed to prevent the spread of this viral infection in Albania.BHV-1,

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