scholarly journals Stem cells in the amniotic fluid: the new chance of regenerative medicine

2011 ◽  
Vol 152 (15) ◽  
pp. 581-587 ◽  
Author(s):  
József Gábor Joó
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Amniotic Fluid ◽  
Diagnostic Tool ◽  
Congenital Malformations ◽  
Genetic Diseases ◽  
Storage Diseases ◽  
Amniotic Fluid Stem Cells ◽  
Amniotic Fluid Stem Cell ◽  
Near Future

Amniotic fluid has been used in prenatal diagnosis for more than decades. It yields a simple and reliable screening and diagnostic tool for a variety of congenital malformations and genetic diseases such as chromosomal aberrations, neural tube defects or storage diseases. Nowadays the widening knowledge provides evidence that amniotic fluid is not only a screening and diagnostic tool, but it may be also the source of the effective therapy of several congenital and adult disorders. A subset of cells, the so-called stem cells were found in the amniotic fluid as well as the placenta, and they proved to be capable of maintaining prolonged undifferentiated proliferation. Stem cells are able to differentiate into multiple tissue types, originating from the three germ layers. In the near future stem cells isolated from amniotic fluid or placenta and stored by cryopreservation may play a significant role in regenerative medicine. Congenital malformations as well as certain diseases in adults might be treated by tissues coming from progenitor cells of amniotic fluid stem cell origin. This study gives a summary of the main characteristics of amniotic fluid stem cells and it also presents important examples of their possible clinical application. Orv. Hetil., 2011, 152, 581–587.

scholarly journals Amniotic Fluid Stem Cells: A New Era in Regenerative Medicine

2017 ◽  
Vol 68 (1) ◽  
pp. 15-19 ◽  
Author(s):  
Mala Srivastava ◽  
Neha Ahlawat ◽  
Ankita Srivastava
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Amniotic Fluid ◽  
New Era ◽  
Amniotic Fluid Stem Cells

scholarly journals Renal differentiation of amniotic fluid stem cells: perspectives for clinical application and for studies on specific human genetic diseases

2011 ◽  
Vol 42 (6) ◽  
pp. 677-684 ◽  
Author(s):  
Margit Rosner ◽  
Katharina Schipany ◽  
Claudia Gundacker ◽  
Bharanidharan Shanmugasundaram ◽  
Kongzhao Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Clinical Application ◽  
Genetic Diseases ◽  
Amniotic Fluid Stem Cells ◽  
Renal Differentiation

Engineering tissue for the fetus: stem cells and matrix signalling

2014 ◽  
Vol 42 (3) ◽  
pp. 631-635 ◽  
Author(s):  
Paolo De Coppi
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Congenital Malformation ◽  
Congenital Malformations ◽  
Adult Stem Cells ◽  
Neonatal Period ◽  
Amniotic Fluid Stem Cells ◽  
The Future ◽  
Causes Of Disease ◽  
Functional Replacement

Congenital malformations are major causes of disease and death during the first years of life and, most of the time, functional replacement of the missing or damaged organs remains an unmet clinical need. Particularly relevant for the treatment of congenital malformation would be to collect the stem cells at diagnosis, before birth, to be able to intervene during the gestation or in the neonatal period. Human AFSCs (amniotic fluid stem cells), which have characteristics intermediate between those of embryonic and adult stem cells, have been isolated. c-Kit+Lin− cells derived from amniotic fluid display a multilineage haemopoietic potential and they can be easily reprogrammed to a pluripotent status. Although, in the future, we hope to use cells derived from the amniotic fluid, we and others have proved recently that simple organs such as the trachea can be engineered using adult progenitors utilizing decellularized cadaveric matrices. A similar approach could be used in the future for more complex organs such as the muscles, intestines or lungs.

scholarly journals Amniotic Fluid Stem Cells: A Novel Source for Modeling of Human Genetic Diseases

2016 ◽  
Vol 17 (4) ◽  
pp. 607 ◽  
Author(s):  
Ivana Antonucci ◽  
Martina Provenzano ◽  
Melissa Rodrigues ◽  
Andrea Pantalone ◽  
Vincenzo Salini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Genetic Diseases ◽  
Amniotic Fluid Stem Cells

scholarly journals Unravelling Heterogeneity of Amplified Human Amniotic Fluid Stem Cells Sub-Populations

Cells ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 158
Author(s):  
Francesca Casciaro ◽  
Silvia Zia ◽  
Mattia Forcato ◽  
Manuela Zavatti ◽  
Francesca Beretti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Amniotic Fluid ◽  
New Technology ◽  
Enrichment Analysis ◽  
Gene Set Enrichment Analysis ◽  
Label Free ◽  
Human Amniotic Fluid ◽  
Significant Differential Expression ◽  
Amniotic Fluid Stem Cells

Human amniotic fluid stem cells (hAFSCs) are broadly multipotent immature progenitor cells with high self-renewal and no tumorigenic properties. These cells, even amplified, present very variable morphology, density, intracellular composition and stemness potential, and this heterogeneity can hinder their characterization and potential use in regenerative medicine. Celector® (Stem Sel ltd.) is a new technology that exploits the Non-Equilibrium Earth Gravity Assisted Field Flow Fractionation principles to characterize and label-free sort stem cells based on their solely physical characteristics without any manipulation. Viable cells are collected and used for further studies or direct applications. In order to understand the intrapopulation heterogeneity, various fractions of hAFSCs were isolated using the Celector® profile and live imaging feature. The gene expression profile of each fraction was analysed using whole-transcriptome sequencing (RNAseq). Gene Set Enrichment Analysis identified significant differential expression in pathways related to Stemness, DNA repair, E2F targets, G2M checkpoint, hypoxia, EM transition, mTORC1 signalling, Unfold Protein Response and p53 signalling. These differences were validated by RT-PCR, immunofluorescence and differentiation assays. Interestingly, the different fractions showed distinct and unique stemness properties. These results suggest the existence of deep intra-population differences that can influence the stemness profile of hAFSCs. This study represents a proof-of-concept of the importance of selecting certain cellular fractions with the highest potential to use in regenerative medicine.

scholarly journals Human amniotic fluid stem cells as a model for functional studies of genes involved in human genetic diseases or oncogenesis

Oncotarget ◽  
2011 ◽  
Vol 2 (9) ◽  
pp. 705-712 ◽  
Author(s):  
Margit Rosner ◽  
Helmut Dolznig ◽  
Katharina Schipany ◽  
Mario Mikula ◽  
Oliver Brandau ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Genetic Diseases ◽  
Human Amniotic Fluid ◽  
Amniotic Fluid Stem Cells ◽  
Functional Studies

Therapeutic Potential of Amniotic Fluid Stem Cells

2013 ◽  
Vol 8 (2) ◽  
pp. 117-124 ◽  
Author(s):  
Hassan Abdulrazzak ◽  
Paolo De Coppi ◽  
Pascale V Guillot
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Therapeutic Potential ◽  
Amniotic Fluid Stem Cells

Amniotic fluid stem cells ameliorate bladder dysfunction induced by chronic bladder ischemia in rat

2017 ◽  
Vol 37 (1) ◽  
pp. 123-131 ◽  
Author(s):  
Ching‐Chung Liang ◽  
Sheng‐Wen Steven Shaw ◽  
Yi‐Hao Lin ◽  
Tsong‐Hai Lee
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Bladder Dysfunction ◽  
Amniotic Fluid Stem Cells ◽  
Bladder Ischemia
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