Characterization and Dynamics of Intracellular Gene Transfer in Plastid Genomes of Viola (Violaceae) and Order Malpighiales

Functional gene transfer from organelles to the nucleus, known as intracellular gene transfer (IGT), is an ongoing process in flowering plants. The complete plastid genomes (plastomes) of two Ulleung island endemic violets, Viola ulleungdoensis and V. woosanensis, were characterized, revealing a lack of the plastid-encoded infA, rpl32, and rps16 genes. In addition, functional replacement of the three plastid-encoded genes in the nucleus was confirmed within the genus Viola and the order Malpighiales. Three strategies for the acquisition of a novel transit peptide for successful IGT were identified in the genus Viola. Nuclear INFA acquired a novel transit peptide with very low identity between these proteins, whereas the nuclear RPL32 gene acquired an existing transit peptide via fusion with the nuclear-encoded plastid-targeted SOD gene (Cu-Zn superoxide dismutase superfamily) as one exon, and translated both proteins in the cytosol using alternative mRNA splicing. Nuclear RPS16 contains an internal transit peptide without an N-terminal extension. Gene loss or pseudogenization of the plastid-borne rpl32 and rps16 loci was inferred to occur in the common ancestor of the genus Viola based on an infrageneric phylogenetic framework in Korea. Although infA was lost in the common ancestor of the order Malpighiales, the rpl32 and rps16 genes were lost multiple times independently within the order. Our current study sheds additional light on plastid genome composition and IGT mechanisms in the violet genus and in the order Malpighiales.

Multiple ancestral duplications of the red-sensitive opsin gene (LWS) in teleost fishes and convergent spectral shifts to green vision in gobies

Photopigments, consisting of an opsin protein bound to a light-sensitive chromophore, are at the centre of vertebrate vision. The vertebrate ancestor already possessed four cone opsin classes involved in colour perception during bright-light conditions, which are sensitive from the ultraviolet to the red-wavelengths of light. Teleosts experienced an extra round of whole genome duplication (3R) at their origin, and while most teleosts maintained only one long-wavelength-sensitive opsin gene (LWS1), the second ancestral copy (LWS2) persisted in characins and osteoglossomorphs. Following 3R, teleost opsins have continued to expand and diversify, which is thought to be a consequence of the different light environment fishes inhabit, from clear streams to the relative darkness of the deep-sea. Although many recent and a few ancestral opsin duplicates can be found, none predating the 3R were thought to exist. In this study we report on a second, previously unnoticed ancestral duplication of the red-sensitive opsin (LWS3), which predates the teleost-specific genome duplication and only persists in gobiid fishes. This is surprising, since it implies that LWS3 has been lost at least 19-20 times independently along the teleost phylogeny. Mining 109 teleost genomes we also uncover a third lineage, the elopomorphs, that maintained the LWS2 copy. We identify convergent amino acid changes that green-shift ancestral and recent LWS copies, leading to adaptive differentiation and the functional replacement of the original green-sensitive RH2 opsin. Retinal transcriptomes and in-situ hybridisation show that LWS3 is expressed to various extents in gobies and in the case of the whitebarred goby, Amblygobius phalaena, it occurs in a separate photoreceptor to LWS1. Our study highlights the importance of comparative studies to comprehend evolution of gene function.

Dual Functional Replacement Syntans for Leather

In the present study we have synthesised thermoresponsive syntan using phase changing material (PCM) encapsulated into a replacement syntan. Syntan was analysed for their particle size, thermal response was verified using Differential scanning calorimetry (DSC) and surface morphology was analysed using scanning electron microscopy (SEM). These syntans were applied to leathers for making leather thermoresponsive. The thermal comfort range achieved on leathers prepared using the experimental syntan was about 2.5℃, compared to control of about 0.5℃ with an error of ±0.5℃. The physical and strength properties of experimental leathers were superior compared to control.

Local environmental variables are key drivers of ant taxonomic and functional beta-diversity in a Mediterranean dryland

The decomposition of beta-diversity (β-diversity) into its replacement (βrepl) and richness (βrich) components in combination with a taxonomic and functional approach, may help to identify processes driving community composition along environmental gradients. We aimed to understand which abiotic and spatial variables influence ant β-diversity and identify which processes may drive ant β-diversity patterns in Mediterranean drylands by measuring the percentage of variation in ant taxonomic and functional β-diversity explained by local environmental, regional climatic and spatial variables. We found that taxonomic and functional replacement (βrepl) primarily drove patterns in overall β-diversity (βtot). Variation partitioning analysis showed that respectively 16.8%, 12.9% and 21.6% of taxonomic βtot, βrepl and βrich variation were mainly explained by local environmental variables. Local environmental variables were also the main determinants of functional β-diversity, explaining 20.4%, 17.9% and 23.2% of βtot, βrepl and βrich variation, respectively. Findings suggest that niche-based processes drive changes in ant β-diversity, as local environmental variables may act as environmental filters on species and trait composition. While we found that local environmental variables were important predictors of ant β-diversity, further analysis should address the contribution of other mechanisms, e.g. competitive exclusion and resource partitioning, on ant β-diversity.

Utilization of Banana (Musa acuminata) Pseudostem for Biscuit Making

The aim of this research was to utilize banana pseudostem powder as a functional replacement ingredient for wheat flour in the production of biscuit. In this study, D-optimal mixture design was employed and five different formulations were obtained. The biscuits were prepared by partially replacing wheat flour with banana pseudostem powder from 0-10% and investigated for sensory and quality attributes. The biscuits were subjected to sensory evaluation by ten semi-trained panelists for consumer acceptability. The data obtained were statistically analyzed using two-way ANOVA (no blocking) at 5% level of significance. From the mean sensory scores, 2.5 parts pseudostem incorporation was selected as the best formulation and subjected for further proximate analysis. The calcium, potassium, sodium, ash, fibre and fat contents were found to be higher in banana pseudostem incorporated biscuit than wheat flour biscuit.

Why cosmopolitan war is an ethics of fantasy?

This article argues that Fabre’s cosmopolitan war is implausible because it ignores the psychological realities of war. Building on J.L. Mackie’s notion of an ‘ethics of fantasy’ – a morality reduced to lip-service and incapable of action-guiding – I argue that a view based on a flawed view of either human agency or the context in which it is exercised is doomed to practical irrelevance. In rejecting patriotism and advancing a highly individualistic view of war, Fabre relies upon a highly flawed view of human agency, ignoring the psychological mechanism of depersonalisation essential to large-scale cooperation and the practice of war. In Part i, I offer an initial account of what an ‘ethics of fantasy’ is and offer one major reason why certain moralities fail as practical guidance. In Part ii, I contrast Fabre’s moral cosmopolitanism with ancient cosmopolitanism; I focus on her rejection of patriotism and other identity-based forms of partiality. In Part iii, I summarise key findings, mostly in social psychology, on how large-scale social cooperation is achieved. I highlight the central role of depersonalisation and its felicity conditions. In Part iv, I argue that Fabre’s view faces a dilemma. Either her cosmopolitanism is compatible with identity-based partiality, or it is not. If not, then she does not even have a view of war given that large-scale cooperation requires it. If her view is compatible, then she needs a functional replacement for patriotism. I conclude by showing that the alternatives fail to satisfy the felicity conditions of depersonalisation which war requires.

REPRESENTATION OF THE STRATEGY OF PERSUASION IN THE TRANSLATION OF ENGLISH ADVERTISING TEXTS OF THE BEAUTY INDUSTRY INTO THE UKRAINIAN LANGUAGE

The article is dedicated to the problem of the interdisciplinary character which is also urgent for psycholinguistics and translation studies. In the focus of the analysis there are linguistic means of representing persuasion strategy in English advertising texts in beauty-industry and the key translation strategies, tactics and devices. It has been concluded that the dominant ones are the strategy of re-addressing and of tertiary translation, while the most widely occurred tactics are the tactic of rendering relevant information, the tactic correct presentation and the tactic of linguistic and cultural adaptation. The analysis of the translation devices has revealed the predominance of such simple devices as transplantation, transcoding, different types of replacements (contextual replacement, concretization, functional replacement). On the level of complex translation transformations there has been resumed that the most widely used are descriptive translation, antonymous translation and the technique of semantic development. The research is perspective and its findings may be employed as a means of translation optimization in the field of beauty industry.

Regulatory gene function handoff allows essential gene loss in mosquitoes

Regulatory genes are often multifunctional and constrained, which results in evolutionary conservation. It is difficult to understand how a regulatory gene could be lost from one species’ genome when it is essential for viability in closely related species. The gene paired is a classic Drosophila pair-rule gene, required for formation of alternate body segments in diverse insect species. Surprisingly, paired was lost in mosquitoes without disrupting body patterning. Here, we demonstrate that a paired family member, gooseberry, has acquired paired-like expression in the malaria mosquito Anopheles stephensi. Anopheles-gooseberry CRISPR-Cas9 knock-out mutants display pair-rule phenotypes and alteration of target gene expression similar to what is seen in Drosophila and beetle paired mutants. Thus, paired was functionally replaced by the related gene, gooseberry, in mosquitoes. Our findings document a rare example of a functional replacement of an essential regulatory gene and provide a mechanistic explanation of how such loss can occur.