Paper-Based Plasmonic Surface for Chemical Biosensing by the Attenuated Total Reflection Method

MRS Advances ◽  
2017 ◽  
Vol 2 (42) ◽  
pp. 2303-2308 ◽  
Author(s):  
Nobuko Fukuda ◽  
Srimongkon Tithimanan ◽  
Hirobumi Ushijima ◽  
Noritaka Yamamoto
Keyword(s):  
Protein Detection ◽  
Gold Surface ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Reflection Method ◽  
Paper Substrate ◽  
Coated Paper ◽  
Coated Papers ◽  
Atomic Force ◽  
Si Wafer

ABSTRACTWe demonstrate the detection of an increase in refractive index and/or thickness by specific adsorption of proteins on a plasmonic surface on a paper substrate in the Otto configuration. Propagating surface plasmon resonance is observed on a gold surface deposited onto polymer-coated papers through angular scans of reflectivity in the Otto configuration under attenuated total reflection conditions. According to a surface analysis with atomic force microscope, the gold surface roughness on a polyvinyl chloride (PVC)-coated paper is comparable to that of a Si wafer, leading to the achievement of protein detection. On the other hand, the propagating length of the surface plasmons is shorter than that on the Si wafer. According to an observation of the gold surface with scanning electron microscope, the gold grain size on the PVC-coated paper is smaller than that on the Si wafer. Thus, many boundaries cause a reduction in the propagating length on the PVC-coated paper.

A Novel Cell Co-Culture Method Utilizing Thermo-Responsive Surface

2007 ◽  
Vol 342-343 ◽  
pp. 221-224
Author(s):  
Jin Suk Bae ◽  
Ga Young Jun ◽  
Akihiko Kikuchi ◽  
Teruo Okano ◽  
Chang Hyun Ahn ◽  
...  
Keyword(s):  
Contact Angle ◽  
Electron Spectroscopy ◽  
Electron Beam Irradiation ◽  
Culture Method ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Beam Irradiation ◽  
Cover Glass ◽  
Force Microscopy ◽  
Atomic Force

In this work, we developed a novel patterned co-culture method with thermo-responsive poly(N-isopropylacrylamide) (PIPAAm) and poly(N-ρ-vinylbenzyl-Ο-β-D-galactopyranosyl-(1→ 4)-D-gluconamide) (PVLA) inducing active hepatocyte attachment. Patterned graft of PIPAAm onto PS dishes was carried out by electron beam irradiation using cover-glass as a photomask. PVLA was only coated onto PIPAAm-ungrafted domain because of hydrated hydrophilic property of PIPAAm at below the LCST. Analysis by attenuated total reflection-Fourier transform infrared and electron spectroscopy for chemical analysis revealed that PIPAAm and PVLA were successfully grafted and coated on surfaces of PS dishes. PIPAAm-grafted surface exhibited decreasing contact angle by changing temperature from 37 to 20°C, while PVLA-coated PS and non-treated PS had negligible contact angle changes with temperature alternation. Atomic force microscopy (AFM) results showed that PIPAAm-grafted and PVLA-coated PS had smoother surfaces than that of ungrafted PS dishes. After culture for 12 hours, hepatocytes were well attached on PVLA-coated domain. Hepatocytes adherent on PIPAAm-grafted domain were detached by decreasing temperature. And then, fibroblasts were seeded onto PIPAAm pattern-grafted domain. Fibroblasts were only attached and spread onto PIPAAm-grafted domain. Co-cultured hepatocytes showed better differentiated function of albumin expression compared to homotypic hepatocyte culture

Exploration for Identification of Sheath-Core Fiber of Ploymer

2014 ◽  
Vol 988 ◽  
pp. 8-12
Author(s):  
Rui Tian Zhu ◽  
Peng Zhang ◽  
Feng Ming Nie
Keyword(s):  
Infrared Spectroscopy ◽  
Accurate Method ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Polymer Fiber ◽  
Reflection Method ◽  
Scanning Calorimetry ◽  
Microscopic Method ◽  
Transmission Technique ◽  
Core Fiber

In this paper, microscopic method, transmission technique and attenuated total reflection method of infrared spectroscopy and differential scanning calorimetry were investigated for analyzing the component of inner and outer layer of sheath-core polymer fiber. Results showed that transmission technique and attenuated total reflection method of infrared spectroscopy was a quick and accurate method for identification of sheath-core fiber of fiber.

Photo-Induced Properties of Liquid Crystal Molecules on Alternately Assembled Charged Azobenzene Dyes and Polyelectrolyte Films Using Attenuated Total Reflection Method

1999 ◽  
Vol 598 ◽  
Author(s):  
Akira Baba ◽  
Takashi Kato ◽  
Jun Ishikawa ◽  
Futao Kaneko ◽  
Kazunari Shinbo ◽  
...  
Keyword(s):  
Liquid Crystal ◽  
Self Assembly ◽  
Surface Plasmon Polariton ◽  
Hybrid Cell ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Low Molecular Weight ◽  
Reflection Method ◽  
Linearly Polarized ◽  
Plasmon Polariton

ABSTRACTIn this report, we describe the photo-induced in-plane alignment of nematic liquid crystal (LC) molecules, 5CB, in a hybrid cell with alternate self-assembled films of low-molecular weight azobenzene dye and polyelectrolyte on gold electrodes using the attenuated total reflection (ATR) measurement method. The molecular self-assembly process was also investigated by the ATR method. Reflectivities due to excitations of the surface plasmon polariton (SPP) were observed in the ATR properties of the LC cell. In-plane switching of the LC molecules adjacent to the aligning layer was evaluated from the ATR curves after irradiation of linearly polarized visible light to the LC cell. The sensitivity of the ATR method gives information on the alignment, tilt angle, and extent of LC reorientation in the bulk. Thus, the importance of using the molecular self-assembly processes of individual polyion-dye pairs for fabrication of new photo-aligning layers is emphasized.

Enhanced photoluminescence spectroscopy for thin films using the attenuated total reflection method

2011 ◽  
Vol 50 (5) ◽  
pp. 696 ◽  
Author(s):  
Takashi Wakamatsu ◽  
Tadaaki Kitami ◽  
Tomoaki Maruyama ◽  
Susumu Toyoshima
Keyword(s):  
Thin Films ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Photoluminescence Spectroscopy ◽  
Reflection Method ◽  
Enhanced Photoluminescence

Combined in situ atomic force microscopy and infrared attenuated total reflection spectroelectrochemistry

The Analyst ◽  
2013 ◽  
Vol 138 (22) ◽  
pp. 6746 ◽  
Author(s):  
Daniel Neubauer ◽  
Jochen Scharpf ◽  
Alberto Pasquarelli ◽  
Boris Mizaikoff ◽  
Christine Kranz
Keyword(s):  
Atomic Force Microscopy ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Force Microscopy ◽  
Atomic Force
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