Gelation Characteristics and Encapsulation of Stromal Cells in Star Acrylate-Functionalized Poly(ethylene glycol-co-lactide) Macromonomers

2012 ◽  
Vol 1403 ◽  
Author(s):  
Seyedsina Moeinzadeh ◽  
Danial Barati ◽  
Xuezhong He ◽  
Esmaiel Jabbari
Keyword(s):  
Ethylene Glycol ◽  
Storage Modulus ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Polymer Concentration ◽  
Gelation Time ◽  
High Modulus ◽  
Poly Ethylene Glycol ◽  
Increasing Trend ◽  
Poly Ethylene

ABSTRACTIn this work, a novel star 4-arm poly(ethylene glycol-co-lactide) acrylate macromonomer (SPELA) is synthesized, and the effect of macromonomer concentration and architecture on modulus, swelling ratio and sol fraction is investigated. The results show that the storage modulus of the hydrogel had an increasing trend with polymer concentration. Changing the polymer architecture from linear to 4-arm increased the storage modulus by 2.2-fold. The water content depended on the hydrophilic segment density as well as the extent of crosslinking and showed a decreasing trend with macromonomer concentration. The sol fractions of the SPELA hydrogels changed from 13% to 5% when concentration increased from 10% to 25%. The star SPELA hydrogel with high modulus, fast gelation time, and low sol fraction is potential useful as a degradable carrier in cell-based therapies. Results show that the SPELA hydrogel supports viability and osteogenic differentiation of the encapsulated bone marrow stromal cells.

scholarly journals Methoxy-Poly(ethylene glycol) Modified Poly(L-lactide) Enhanced Cell Affinity of Human Bone Marrow Stromal Cells by the Upregulation of 1-Cadherin and Delta-2-catenin

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Xueli Mao ◽  
Zetao Chen ◽  
Junqi Ling ◽  
Jingjing Quan ◽  
Hui Peng ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Adhesion ◽  
Ethylene Glycol ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Poly Ethylene Glycol ◽  
Copolymer Films ◽  
Cell Affinity ◽  
Poly Ethylene

Poly(l-lactide) (PLLA), a versatile biodegradable polymer, is one of the most commonly-used materials for tissue engineering applications. To improve cell affinity for PLLA, poly(ethylene glycol) (PEG) was used to develop diblock copolymers. Human bone marrow stromal cells (hBMSCs) were cultured on MPEG-b-PLLA copolymer films to determine the effects of modification on the attachment and proliferation of hBMSC. The mRNA expression of 84 human extracellular matrix (ECM) and adhesion molecules was analyzed using RT-qPCR to understand the underlying mechanisms. It was found that MPEG-b-PLLA copolymer films significantly improved cell adhesion, extension, and proliferation. This was found to be related to the significant upregulation of two adhesion genes, CDH1 and CTNND2, which encode 1-cadherin and delta-2-catenin, respectively, two key components for the cadherin-catenin complex. In summary, MPEG-b-PLLA copolymer surfaces improved initial cell adhesion by stimulation of adhesion molecule gene expression.

Thermally Cross-Linked Oligo(poly(ethylene glycol) fumarate) Hydrogels Support Osteogenic Differentiation of Encapsulated Marrow Stromal Cells In Vitro

2004 ◽  
Vol 5 (1) ◽  
pp. 5-10 ◽  
Author(s):  
Johnna S. Temenoff ◽  
Hansoo Park ◽  
Esmaiel Jabbari ◽  
Daniel E. Conway ◽  
Tiffany L. Sheffield ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Marrow Stromal Cells ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals rAAV-Mediated Overexpression of SOX9 and TGF-β via Carbon Dot-Guided Vector Delivery Enhances the Biological Activities in Human Bone Marrow-Derived Mesenchymal Stromal Cells

Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 855 ◽  
Author(s):  
Weikun Meng ◽  
Ana Rey-Rico ◽  
Mickaël Claudel ◽  
Gertrud Schmitt ◽  
Susanne Speicher-Mentges ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Citric Acid ◽  
Ethylene Glycol ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Monomethyl Ether ◽  
Poly Ethylene Glycol ◽  
Ethylene Glycol Monomethyl Ether ◽  
Glycol Monomethyl Ether ◽  
Poly Ethylene

Scaffold-assisted gene therapy is a highly promising tool to treat articular cartilage lesions upon direct delivery of chondrogenic candidate sequences. The goal of this study was to examine the feasibility and benefits of providing highly chondroreparative agents, the cartilage-specific sex-determining region Y-type high-mobility group 9 (SOX9) transcription factor or the transforming growth factor beta (TGF-β), to human bone marrow-derived mesenchymal stromal cells (hMSCs) via clinically adapted, independent recombinant adeno-associated virus (rAAV) vectors formulated with carbon dots (CDs), a novel class of carbon-dominated nanomaterials. Effective complexation and release of a reporter rAAV-lacZ vector was achieved using four different CDs elaborated from 1-citric acid and pentaethylenehexamine (CD-1); 2-citric acid, poly(ethylene glycol) monomethyl ether (MW 550 Da), and N,N-dimethylethylenediamine (CD-2); 3-citric acid, branched poly(ethylenimine) (MW 600 Da), and poly(ethylene glycol) monomethyl ether (MW 2 kDa) (CD-3); and 4-citric acid and branched poly(ethylenimine) (MW 600 Da) (CD-4), allowing for the genetic modification of hMSCs. Among the nanoparticles, CD-2 showed an optimal ability for rAAV delivery (up to 2.2-fold increase in lacZ expression relative to free vector treatment with 100% cell viability for at least 10 days, the longest time point examined). Administration of therapeutic (SOX9, TGF-β) rAAV vectors in hMSCs via CD-2 led to the effective overexpression of each independent transgene, promoting enhanced cell proliferation (TGF-β) and cartilage matrix deposition (glycosaminoglycans, type-II collagen) for at least 21 days relative to control treatments (CD-2 lacking rAAV or associated to rAAV-lacZ), while advantageously restricting undesirable type-I and -X collagen deposition. These results reveal the potential of CD-guided rAAV gene administration in hMSCs as safe, non-invasive systems for translational strategies to enhance cartilage repair.

Rheological Behavior of Binary Aqueous Solutions of Poly(ethylene glycol) of 1500 g·mol–1 as Affected by Temperature and Polymer Concentration

2013 ◽  
Vol 58 (4) ◽  
pp. 838-844 ◽  
Author(s):  
Bernardo de Sá Costa ◽  
Jane Sélia dos Reis Coimbra ◽  
Márcio Arêdes Martins ◽  
Edwin Elard Garcia-Rojas ◽  
Javier Telis-Romero ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Aqueous Solutions ◽  
Rheological Behavior ◽  
Polymer Concentration ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals Associative properties of poly(ethylene glycol)–poly(vinyl acetate) comb-like graft copolymers in water

Nanoscale ◽  
2019 ◽  
Vol 11 (14) ◽  
pp. 6635-6643 ◽  
Author(s):  
Marianna Mamusa ◽  
Paolo Tempesti ◽  
Arianna Bartolini ◽  
Emiliano Carretti ◽  
Ahmad F. Ghobadi ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Vinyl Acetate ◽  
Graft Copolymers ◽  
Polymer Concentration ◽  
Complex Structures ◽  
Single Chain ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

A PEG-graft-PVAc comb-like copolymer in water forms spherical single-chain nanoparticles that transition towards hierarchically complex structures with increasing polymer concentration.

Preparation and characterization of hydrogels based on acryloyl end-capped four-arm star-shaped poly(ethylene glycol)-branched-oligo(l-lactide) via Michael-type addition reaction

2010 ◽  
Vol 64 (5) ◽  
Author(s):  
Huai-Qing Yu ◽  
Rimin Cong
Keyword(s):  
Ethylene Glycol ◽  
Addition Reaction ◽  
Gelation Time ◽  
Precursor Solution ◽  
Poly Ethylene Glycol ◽  
Biomedical Material ◽  
Michael Type Addition ◽  
Degradation Behaviors ◽  
Poly Ethylene

AbstractAn acryloyl end-capped four-arm star-shaped poly(ethylene glycol)-branched-oligo(l-lactide) (4A-PEG-PLA) macromer was firstly prepared. A novel kind of hydrogels was synthesized via the Michael-type addition reaction between (2S,3S)-1,4-bis-sulfanylbutane-2,3-diol (dithiothreitol) and this macromer. Gelation time was determined visually as the time when the precursor solution did not flow on inverting the vials. Hydrogel structure was characterized by FTIR analysis, swelling and degradation tests. It was found that colorless and transparent hydrogels were quickly generated in situ. The gelation time, swelling and degradation behaviors of this kind of hydrogels could be adjusted by changing the concentration of the macromer solution in PBS buffer (pH 7.4). This novel hydrogel is expected to be used as a biomedical material.

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