Silicon Microchannel Array as a Basis of Biosensor Device

2006 ◽  
Vol 915 ◽  
Author(s):  
Alexander Lomzov ◽  
Natalia Vandysheva ◽  
Alexander Bublikov ◽  
Dmitry Pyshnyi ◽  
Sergei Romanov
Keyword(s):  
Dna Hybridization ◽  
Direct Analysis ◽  
Infrared Region ◽  
Uv Spectra ◽  
Optical Methods ◽  
Standard Glass ◽  
Microchannel Array ◽  
Glass Slides ◽  
3D Solid ◽  
Silicon Based

AbstractThis work shows the possibilities of using the silicon-based microchannel array (Si-MCA) as a 3D-solid support of DNA probes and studying of application of optical methods for DNA hybridization direct analysis. It was obtained that amount of immobilized oligodeoxyribonucleotides on the Si-MCA surface is enhanced with lighting of Si-MCA microchannel. The sample of 160 μm thickness succeeded in immobilizing oligonucleotides 40 times as much as the standard glass slides having the same external surface. The application of Si-MCA as a carrier of DNA allows doing direct analysis of nucleic acids in infrared region by the FTIR method that essentially simplifies creation of a biosensor based on the Si-MCA. It is indicated that using of the Si-MCA as a diffraction grating in visible and UV spectra allows simplifying the spectral analysis of biochemical reaction. It was success confidently to distinguish the mediums differing on the refractive index by dn=0.006.

Diagnostics of quantum cascade structures by optical methods in the near infrared region

2012 ◽  
Vol 46 (11) ◽  
pp. 1411-1414 ◽  
Author(s):  
D. I. Kryzhkov ◽  
S. V. Morozov ◽  
D. M. Gaponova ◽  
S. M. Sergeev ◽  
K. I. Kuritsyn ◽  
...  
Keyword(s):  
Near Infrared ◽  
Infrared Region ◽  
Optical Methods ◽  
Quantum Cascade ◽  
Near Infrared Region ◽  
Quantum Cascade Structures

scholarly journals Optical Transport Properties of Graphene Surface Plasmon Polaritons in Mid-Infrared Band

Crystals ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 354 ◽  
Author(s):  
Yindi Wang ◽  
Hongxia Liu ◽  
Shulong Wang ◽  
Ming Cai ◽  
Lan Ma
Keyword(s):  
Surface Plasmon ◽  
Surface Plasmon Polaritons ◽  
Infrared Region ◽  
Transmission Characteristics ◽  
Infrared Band ◽  
Theoretical Derivation ◽  
Mid Infrared ◽  
Graphene Surface ◽  
Silicon Based ◽  
Plasmon Polaritons

The excellent transmission characteristics of graphene surface plasmon polaritons in mid-infrared band were analyzed and verified effectively through theoretical derivation and soft simulation in this paper. Meanwhile, a sandwich waveguide structure of dielectric–graphene–substrate–dielectric based on graphene surface plasmon polaritons (SPPs) was presented. Simulation results indicate that graphene SPPs show unique properties in the mid-infrared region including ultra-compact mode confinement and dynamic tunability, which allow these SPPs to overcome the defects of metal SPPs and traditional silicon-based optoelectronic devices. Thus, they can be used to manufacture subwavelength devices. The work in this paper lays a theoretical foundation for the application of graphene SPPs in the mid-infrared region.

Synthesis of Silicon Based Opal by Chemical Reduction of Silica Opal

2006 ◽  
Vol 964 ◽  
Author(s):  
Ali E. Aliev ◽  
M. A. O. Royer ◽  
A. A. Zakhidov ◽  
R. H. Baughman
Keyword(s):  
Photonic Crystal ◽  
Elemental Analysis ◽  
Chemical Reduction ◽  
Contrast Ratio ◽  
Infrared Region ◽  
Gas Mixture ◽  
Sem Images ◽  
Long Wavelength ◽  
Dielectric Contrast ◽  
Silicon Based

ABSTRACTBy reduction of SiO2 opal in atmosphere of nitrogen (80%)-helium (15%)-hydrogen (5%) gas mixture at various temperatures we tuned the dielectric contrast ratio and band gap of photonic crystal (PC) up to 10 %. The SEM images dose not show any detectible change in lattice parameters, whereas the elemental analysis shows remarkable decrease of oxygen content. The shift of reflectance spectra toward the long wavelength and increase of transmission in infrared region confirm the redaction of silica and partial transformation to the silicon.

scholarly journals The development of crosslinked gelatin thin films as controlled release matrices

2021 ◽  
Author(s):  
Alireza Abbasi
Keyword(s):  
Thin Films ◽  
Mathematical Model ◽  
Controlled Release ◽  
Release Kinetics ◽  
Fluorescent Marker ◽  
Force Microscopy ◽  
Standard Glass ◽  
Atomic Force ◽  
Gelatin Films ◽  
Glass Slides

The aim of this thesis was to develop gelatin-based thin films that may be used as matrices for the controlled release of bioactive compounds. There were three objectives to this research: i) develop a method to generate the thin films ii) experimentally quantify the release of a fluorescent marker from these films, and iii) implement a mathematical model to characterize the release of the fluorescent marker. To achieve the first objective, a novel method of developing thin films was implemented where sub-micron thickness films affixed to standard glass slides were crosslinked with genipin, a naturally-occurring fixative. Gel thickness measured using atomic force microscopy (AFM) varied from 350 to 650 nm irrespective of the concentration of genifin added. AFM-based surface roughness decreased with increasing genipin concentration. Release behavior of a fluorescent marker from the thin films demonstrated a strong influence of genipin concentration on release kinetics, with greater genipin leading to slower release. A mathematical model for the water transport into, and fluorescent marker release from, the genipin-crosslinked gelatin films was developed and successfully implemented with both the water ingress (swelling) and marker release being effectively characterized by the model.

scholarly journals The development of crosslinked gelatin thin films as controlled release matrices

2021 ◽  
Author(s):  
Alireza Abbasi
Keyword(s):  
Thin Films ◽  
Mathematical Model ◽  
Controlled Release ◽  
Release Kinetics ◽  
Fluorescent Marker ◽  
Force Microscopy ◽  
Standard Glass ◽  
Atomic Force ◽  
Gelatin Films ◽  
Glass Slides

The aim of this thesis was to develop gelatin-based thin films that may be used as matrices for the controlled release of bioactive compounds. There were three objectives to this research: i) develop a method to generate the thin films ii) experimentally quantify the release of a fluorescent marker from these films, and iii) implement a mathematical model to characterize the release of the fluorescent marker. To achieve the first objective, a novel method of developing thin films was implemented where sub-micron thickness films affixed to standard glass slides were crosslinked with genipin, a naturally-occurring fixative. Gel thickness measured using atomic force microscopy (AFM) varied from 350 to 650 nm irrespective of the concentration of genifin added. AFM-based surface roughness decreased with increasing genipin concentration. Release behavior of a fluorescent marker from the thin films demonstrated a strong influence of genipin concentration on release kinetics, with greater genipin leading to slower release. A mathematical model for the water transport into, and fluorescent marker release from, the genipin-crosslinked gelatin films was developed and successfully implemented with both the water ingress (swelling) and marker release being effectively characterized by the model.

scholarly journals Digital Whole Slide Imaging Compared With Light Microscopy for Primary Diagnosis in Surgical Pathology

2020 ◽  
Vol 144 (10) ◽  
pp. 1245-1253 ◽  
Author(s):  
Alexander D. Borowsky ◽  
Eric F. Glassy ◽  
William Dean Wallace ◽  
Nathash S. Kallichanda ◽  
Cynthia A. Behling ◽  
...  
Keyword(s):  
Digital Pathology ◽  
Diagnostic Category ◽  
Primary Diagnosis ◽  
Current Standard ◽  
Diagnostic Categories ◽  
Standard Glass ◽  
Specific Subset ◽  
Glass Slides ◽  
Major Discrepancy ◽  
Whole Slide Images

Context.— The adoption of digital capture of pathology slides as whole slide images (WSI) for educational and research applications has proven utility. Objective.— To compare pathologists' primary diagnoses derived from WSI versus the standard microscope. Because WSIs differ in format and method of observation compared with the current standard glass slide microscopy, this study is critical to potential clinical adoption of digital pathology. Design.— The study enrolled a total of 2045 cases enriched for more difficult diagnostic categories and represented as 5849 slides were curated and provided for diagnosis by a team of 19 reading pathologists separately as WSI or as glass slides viewed by light microscope. Cases were reviewed by each pathologist in both modalities in randomized order with a minimum 31-day washout between modality reads for each case. Each diagnosis was compared with the original clinical reference diagnosis by an independent central adjudication review. Results.— The overall major discrepancy rates were 3.64% for WSI review and 3.20% for manual slide review diagnosis methods, a difference of 0.44% (95% CI, −0.15 to 1.03). The time to review a case averaged 5.20 minutes for WSI and 4.95 minutes for glass slides. There was no specific subset of diagnostic category that showed higher rates of modality-specific discrepancy, though some categories showed greater discrepancy than others in both modalities. Conclusions.— WSIs are noninferior to traditional glass slides for primary diagnosis in anatomic pathology.

scholarly journals Fluorescence Enhancement on Silver-Plated Plasma Micro-Nanostructured 3D Polymeric Microarray Substrates for Multiplex Mycotoxin Detection

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 392
Author(s):  
Georgios Koukouvinos ◽  
Chrysoula-Evangelia Karachaliou ◽  
Anastasia Kanioura ◽  
Katerina Tsougeni ◽  
Evangelia Livaniou ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Fluorescence Intensity ◽  
Aflatoxin B1 ◽  
Oxygen Plasma ◽  
Fluorescence Enhancement ◽  
Fumonisin B1 ◽  
Silver Deposition ◽  
Standard Glass ◽  
Glass Slides ◽  
Coefficients Of Variation

Oxygen plasma micro-nanostructured poly(methyl methacrylate) (PMMA) slides were modified through silver microparticle deposition to create microarray substrates that enhance the emitted fluorescence intensity. Silver deposition relied on a commercially available reagent and was completed in two 30-min incubation cycles of the substrate with the reagent. The fluorescence enhancement achieved using these substrates over flat PMMA slides was determined through the development of a microarray for the multiplexed detection of four mycotoxins, aflatoxin B1, ochratoxin A, fumonisin B1, and deoxynivalenol. It was shown that the implementation of silver-plated oxygen plasma micro-nanotextured PMMA substrates increased the signals obtained for aflatoxin B1 and ochratoxin A by approximately 2.8 times, 5.6 times for deoxynivalenol, and 16-times for fumonisin B1, compared to flat PMMA substrates. Most notably, this signal increase was not accompanied by a significant increase in the non-specific signal. In addition, the spot repeatability both across a single slide as well as between different slides was high, with coefficients of variation lower than 12%. The slides were also stable for at least three months, thus offering a microarray substrate with improved properties compared to standard glass slides, regarding both the absolute spot fluorescence intensity and between spots repeatability.

A porous silicon based microarray for label-free optical detection of DNA hybridization

2010 ◽  
Author(s):  
Ilaria Rea ◽  
Giuseppe Coppola ◽  
Mariano Gioffrè ◽  
Edoardo De Tommasi ◽  
Ivo Rendina ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Dna Hybridization ◽  
Optical Detection ◽  
Label Free ◽  
Silicon Based

Procedures for recovering mesospheric and stratospheric temperatures from OSIRIS scattered-sunlight measurements

2002 ◽  
Vol 80 (4) ◽  
pp. 435-442 ◽  
Author(s):  
C S Haley ◽  
I C McDade
Keyword(s):  
Near Infrared ◽  
Direct Analysis ◽  
Infrared Region ◽  
Temperature Profiles ◽  
Algorithm Performance ◽  
Recovery Algorithm ◽  
Stratospheric Temperature ◽  
Infrared Imager ◽  
Instrument Noise ◽  
Better Than

Procedures for recovering mesospheric and stratospheric temperature profiles from scattered-sunlight limb radiance measurements made by the OSIRIS (optical spectrograph and infrared imager system) instrument on the Odin satellite are described. We assess various approaches to the problem and show that temperature recoveries based on an analysis of inferred volume-scattering rates are significantly more accurate than those based on direct analysis of the observed limb radiances. A forward model for the OSIRIS instrument is used to test temperature recovery algorithm performance in the presence of realistic instrument noise and to assess the expected accuracy of the OSIRIS temperature recoveries. It is shown that, for radiances measured in the near-infrared region, temperatures can be reliably recovered over the altitude range 40 km to 80 km with an accuracy of better than ±1 K. PACS Nos.: 42.68Mj, 94.10Dy

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