NanoCT: Visualizing of internal 3D-Structures with Submicrometer Resolution

2007 ◽  
Vol 990 ◽  
Author(s):  
Oliver Brunke ◽  
Dirk Neuber ◽  
David K. Lehmann
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Volume Data ◽  
High Resolution Computed Tomography ◽  
Data Set ◽  
Minimal Sample ◽  
Synthetic Materials ◽  
Volume Visualisation ◽  
Ct System

ABSTRACTHigh-resolution Computed Tomography (CT) widely expands the spectrum of detectable internal micro-structures. The new nanotom CT system allows the analysis of samples with the exceptional voxel-resolution of less than 0.5 microns per volume pixel (voxel). Thus internal detail related to a variation in material, density or porosity can be visualised and precisely measured. This opens a new dimension of 3D-microanalysis and will partially substitute traditional destructive methods in industrial quality control and research.The nanotom® is the first 180 kV nanoCT system world-wide which is tailored specifically to the highest-resolution applications in material science, micro electronics, geology and biology. Therefore it is particularly suitable for nanoCT-examinations of material samples of any type like synthetic materials, ceramics, composite materials, mineral and organic samples.Several results of high-resolution nanoCT demonstrate the capability to analyse the 3D-microstructure of materials with only minimal sample preparation. For instance, it is possible to image different metal phases in solder joints, the texture of fibres in composites or the volume and distribution of voids in castings.The volume data set is visualised by slices or compiled in a three-dimensional view which can be displayed in various ways. By means of volume visualisation software, the three-dimensional structure of the reconstructed volume can be easily analysed for pores, cracks, and materials density and distribution with the highest magnification and image quality available.By granting the user the ability to navigate the internal structure of an object slice-by-slice with highest resolution in a non-destructive manner, the nanotom creates new possibilities for analysis which have so far been unreachable.

Refinement of the three-dimensional structure of PhoE porin at 3.2Å resolution as determined by electron crystallography

1994 ◽  
Vol 52 ◽  
pp. 96-97
Author(s):  
Bing K. Jap ◽  
Peter Walian ◽  
Thomas Earnest
Keyword(s):  
High Resolution ◽  
Coulomb Potential ◽  
Molecular Model ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Phase Information ◽  
Electron Crystallography ◽  
Data Set ◽  
Crystallographic Symmetry ◽  
Phoe Porin

Density modification methods were used to improve the three-dimensional map of PhoE porin which has been obtained by electron crystallographic techniques. The electron crystallographic data set consists of diffraction amplitudes that extend to a resolution of∽ 2.8 Å and phase information derived from 22 tilted and 8 untilted images at 3.5 Å resolution. However the data set was restricted to tilt angles less than ∽ 60 degrees, and therefore there is a missing cone regionin our 3-D data set. Non-crystallographic symmetry averaging and solvent flattening techniques were used to improve and extend the phase information, allowing for the calculation of high resolution Coulomb potential maps of membrane-embedded PhoE. These maps were used as a basis for building a partial molecular model, which was subsequently used to supply phase information to be combined with the experimentally determined phases. The combined phases were then used to calculate improved maps. As the maps continue to improve, more of the backbone and some of the side chains were built into the maps.

Electron crystallographic determination of the 3-D structure of staurolite at atomic resolution

1991 ◽  
Vol 49 ◽  
pp. 540-541
Author(s):  
Kenneth H. Downing ◽  
Hu Meisheng ◽  
Hans-Rudolf Went ◽  
Michael A. O'Keefe
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
High Resolution Electron Microscopy ◽  
Atomic Resolution ◽  
Dimensional Structure ◽  
Structure Information ◽  
Resolution Electron ◽  
Scattering Effects ◽  
High Resolution Images ◽  
Effects Of Radiation

With current advances in electron microscope design, high resolution electron microscopy has become routine, and point resolutions of better than 2Å have been obtained in images of many inorganic crystals. Although this resolution is sufficient to resolve interatomic spacings, interpretation generally requires comparison of experimental images with calculations. Since the images are two-dimensional representations of projections of the full three-dimensional structure, information is invariably lost in the overlapping images of atoms at various heights. The technique of electron crystallography, in which information from several views of a crystal is combined, has been developed to obtain three-dimensional information on proteins. The resolution in images of proteins is severely limited by effects of radiation damage. In principle, atomic-resolution, 3D reconstructions should be obtainable from specimens that are resistant to damage. The most serious problem would appear to be in obtaining high-resolution images from areas that are thin enough that dynamical scattering effects can be ignored.

scholarly journals Automated analysis of three-dimensional xylem networks using high-resolution computed tomography

2011 ◽  
Vol 191 (4) ◽  
pp. 1168-1179 ◽  
Author(s):  
Craig R. Brodersen ◽  
Eric F. Lee ◽  
Brendan Choat ◽  
Steven Jansen ◽  
Ronald J. Phillips ◽  
...  
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
Three Dimensional ◽  
Automated Analysis ◽  
High Resolution Computed Tomography

scholarly journals High-resolution three-dimensional probes of biomaterials and their interfaces

2012 ◽  
Vol 370 (1963) ◽  
pp. 1337-1351 ◽  
Author(s):  
Kathryn Grandfield ◽  
Anders Palmquist ◽  
Håkan Engqvist
Keyword(s):  
High Resolution ◽  
Electron Tomography ◽  
Three Dimensional ◽  
Controlled Drug Release ◽  
Biological Tissues ◽  
Dimensional Structure ◽  
Bone Interface ◽  
Biomaterial Interfaces ◽  
Titania Coating

Interfacial relationships between biomaterials and tissues strongly influence the success of implant materials and their long-term functionality. Owing to the inhomogeneity of biological tissues at an interface, in particular bone tissue, two-dimensional images often lack detail on the interfacial morphological complexity. Furthermore, the increasing use of nanotechnology in the design and production of biomaterials demands characterization techniques on a similar length scale. Electron tomography (ET) can meet these challenges by enabling high-resolution three-dimensional imaging of biomaterial interfaces. In this article, we review the fundamentals of ET and highlight its recent applications in probing the three-dimensional structure of bioceramics and their interfaces, with particular focus on the hydroxyapatite–bone interface, titanium dioxide–bone interface and a mesoporous titania coating for controlled drug release.

scholarly journals Three-dimensional Sonography in the Assessment of Normal Fetal Anatomy in Late Pregnancy

2010 ◽  
Vol 4 (3) ◽  
pp. 217-231
Author(s):  
Badreldeen Ahmed ◽  
Ulrich Honemeyer
Keyword(s):  
Continuous Wave ◽  
Power Doppler ◽  
Three Dimensional ◽  
Late Pregnancy ◽  
Research Field ◽  
Surface Rendering ◽  
Volume Data ◽  
Data Set ◽  
3D Volume ◽  
Color And Power Doppler

Abstract Three-dimensional, multiplanar sonography, using a volume data set acquired with a 3D probe, has revolutionized ultrasonographic imaging and takes sonographers to a new perception of the fetus in 3 dimensions. Real time scanning, until the late nineties only possible in B-mode, can now be performed in 3D with up to 40 frames/sec. Fetal neurology emerged as a new perinatal research field with the 4D visualization of fetal behavior. Doppler ultrasound, diversified and refined from continuous wave and pulsed Doppler to Color – and Power Doppler, when added to 3D sonography, creates fascinating options of noninvasive fetal vascular mapping (sonoangiography) and vascular assessment of placenta. The diagnostic and demonstrative potential of an acquired 3D volume data set can be maxed with the help of postprocessing and rendering software. After storage, the evaluation of fetal 3D data sets can happen without the patient, with the option of specialist consultation, using telemedicine. In the article, the new 3D “modes” like surface rendering, maximum mode, 3D Color and Power Doppler, STIC, volume rendering, and glass body rendering, are described and illustrated in their display of normal fetal anatomy.

scholarly journals Solvent Accessibility of Residues Undergoing Pathogenic Variations in Humans: From Protein Structures to Protein Sequences

2021 ◽  
Vol 7 ◽  
Author(s):  
Castrense Savojardo ◽  
Matteo Manfredi ◽  
Pier Luigi Martelli ◽  
Rita Casadio
Keyword(s):  
Solvent Accessibility ◽  
Protein Structures ◽  
Three Dimensional ◽  
Protein Sequences ◽  
Large Data ◽  
Human Protein ◽  
Dimensional Structure ◽  
Wild Type ◽  
Solvent Exposure ◽  
Data Set

Solvent accessibility (SASA) is a key feature of proteins for determining their folding and stability. SASA is computed from protein structures with different algorithms, and from protein sequences with machine-learning based approaches trained on solved structures. Here we ask the question as to which extent solvent exposure of residues can be associated to the pathogenicity of the variation. By this, SASA of the wild-type residue acquires a role in the context of functional annotation of protein single-residue variations (SRVs). By mapping variations on a curated database of human protein structures, we found that residues targeted by disease related SRVs are less accessible to solvent than residues involved in polymorphisms. The disease association is not evenly distributed among the different residue types: SRVs targeting glycine, tryptophan, tyrosine, and cysteine are more frequently disease associated than others. For all residues, the proportion of disease related SRVs largely increases when the wild-type residue is buried and decreases when it is exposed. The extent of the increase depends on the residue type. With the aid of an in house developed predictor, based on a deep learning procedure and performing at the state-of-the-art, we are able to confirm the above tendency by analyzing a large data set of residues subjected to variations and occurring in some 12,494 human protein sequences still lacking three-dimensional structure (derived from HUMSAVAR). Our data support the notion that surface accessible area is a distinguished property of residues that undergo variation and that pathogenicity is more frequently associated to the buried property than to the exposed one.

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