Local Environment of Surface-Polyelectrolyte-Bound DNA Oligomers

2000 ◽  
Vol 651 ◽  
Author(s):  
Sangmin Jeon ◽  
Sung Chul Bae ◽  
Jiang John Zhao ◽  
Steve Granick
Keyword(s):  
Aqueous Solution ◽  
Salt Concentration ◽  
Fluorescence Anisotropy ◽  
Local Environment ◽  
Time Resolved Fluorescence ◽  
Surface Complexes ◽  
Dna Oligomers ◽  
Time Resolved ◽  
Two Photon ◽  
Anisotropy Decay

AbstractTwo-photon time-resolved fluorescence anisotropy methods were used to study the dynamical environment when fluorescent-labelled DNA oligomers (labelled with FAM, 6-fluorescein-6-carboxamido hexanoate) formed surface complexes with quaternized polyvinylpyridine (QPVP) cationic layers on a glass surface. We compared the anisotropy decay of DNA in bulk aqueous solution, DNA adsorbed onto QPVP, and QPVP-DNA-QPVP sandwich structures. When DNA was adsorbed onto QPVP, its anisotropy decay was dramatically retarded compared to the bulk, which means it had very slow rotational motion on the surface. Motions slowed down with increasing salt concentration up to a level of 0.1 M NaCl, but mobility began to increase at still higher salt concentration owing to detachment from the surface-immobilizing QPVP layers.

Ultrafast pump-probe and time-resolved fluorescence anisotropy study of the intramolecular interaction of branched styryl derivatives based on 1,3,5-triazine

2019 ◽  
Vol 28 (01) ◽  
pp. 1950003 ◽  
Author(s):  
Yaochuan Wang ◽  
Yizhuo Wang ◽  
Guiqiu Wang ◽  
Dajun Liu
Keyword(s):  
Fluorescence Anisotropy ◽  
Intramolecular Interaction ◽  
Intramolecular Interactions ◽  
Photon Absorption ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Pump Probe ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Property Measurement

A series of branched styryl derivatives based on 1,3,5-triazine were studied by nonlinear optical property measurement, degenerated pump-probe, and time-resolved fluorescence anisotropy methods to elucidate the two-photon absorption (TPA) properties and intramolecular interactions between branches. Significant enhancement of the TPA cross-section was observed in multi-branched derivatives. The anisotropy of multi-branched compounds shows faster decay and small residual values, indicating strong intramolecular interactions between branches, which further confirmed the TPA enhancement mechanism.

Orientational distribution of DPH in lipid membranes: a comparison of molecular dynamics calculations and experimental time-resolved anisotropy experiments

2019 ◽  
Vol 21 (14) ◽  
pp. 7594-7604 ◽  
Author(s):  
Markéta Paloncýová ◽  
Marcel Ameloot ◽  
Stefan Knippenberg
Keyword(s):  
Molecular Dynamics ◽  
Fluorescence Anisotropy ◽  
Lipid Membranes ◽  
Time Resolved Fluorescence ◽  
Fluorescence Anisotropy Decay ◽  
Time Resolved ◽  
Anisotropy Decay ◽  
Molecular Dynamics Calculations ◽  
Orientational Distribution

The behavior of the fluorescent probe diphenylhexatriene (DPH) in different lipid phases is investigated. The rotational autocorrelation functions are calculated in order to model the time-resolved fluorescence anisotropy decay. The role of the order parameters is discussed.

scholarly journals Fluorescence anisotropy of oligomeric proteins

2004 ◽  
Vol 18 (1) ◽  
pp. 85-93 ◽  
Author(s):  
Daniel M. Czajkowsky
Keyword(s):  
Fluorescence Anisotropy ◽  
Protein Oligomerization ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Oligomeric Proteins ◽  
Anisotropy Decay ◽  
Oligomeric Complex ◽  
Probe Orientation

Previous studies of protein oligomerization using time-resolved fluorescence anisotropy assumed a single fixed probe per oligomeric complex and an identical probe orientation in complexes of different stoichiometry. However, an oligomer consisting of “n” singly labeled monomers must necessarily have “n” probes. Moreover, in the expression for the anisotropy decay, the molecular axes from which the probe orientation is defined are different for complexes that differ in stoichiometry. Here, we derive an expression for the decay of the anisotropy for molecules with any number of fixed probes, and show how an explicit understanding of the probe orientation is necessary to properly assess oligomerization.

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