Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation

AbstractIn vitro thrombogenicity test systems require co-cultivation of endothelial cells and platelets under blood flow-like conditions. Here, a commercially available perfusion system is explored using plasma-treated cyclic olefin copolymer (COC) as a substrate for the endothelial cell layer. COC was characterized prior to endothelialization and co-cultivation with platelets under static or flow conditions. COC exhibits a low roughness and a moderate hydrophilicity. Flow promoted endothelial cell growth and prevented platelet adherence. These findings show the suitability of COC as substrate and the importance of blood flow-like conditions for the assessment of the thrombogenic risk of drugs or cardiovascular implant materials. Graphic abstract

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In a pig model ePTFE grafts will sustain for 6 weeks a confluent endothelial cell layer formed in vitro under shear stress conditions

European Journal of Vascular and Endovascular Surgery ◽

10.1053/ejvs.2002.1881 ◽

2003 ◽

Vol 26 (2) ◽

pp. 156-160 ◽

Cited By ~ 6

Author(s):

R. Büttemeyer ◽

J.W. Mall ◽

M. Paulitschke ◽

A. Rademacher ◽

A.W. Philipp

Keyword(s):

Shear Stress ◽

Endothelial Cell ◽

Cell Layer ◽

Stress Conditions ◽

Pig Model ◽

Endothelial Cell Layer ◽

Confluent Endothelial Cell

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Interleukin-1β induces an inflammatory response and the breakdown of the endothelial cell layer in an improved human THBMEC-based in vitro blood–brain barrier model

Journal of Neuroscience Methods ◽

10.1016/j.jneumeth.2014.03.002 ◽

2014 ◽

Vol 228 ◽

pp. 35-45 ◽

Cited By ~ 44

Author(s):

Josephine Labus ◽

Sonja Häckel ◽

Lother Lucka ◽

Kerstin Danker

Keyword(s):

Endothelial Cell ◽

Inflammatory Response ◽

Blood Brain Barrier ◽

Cell Layer ◽

Interleukin 1Β ◽

Brain Barrier ◽

Endothelial Cell Layer ◽

Blood Brain Barrier Model ◽

Barrier Model

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Plasmin-induced reduction of heparan sulfate in cultured vascular endothelial cell layer

Thrombosis Research ◽

10.1016/0049-3848(94)90001-9 ◽

1994 ◽

Vol 74 (2) ◽

pp. 85-93 ◽

Cited By ~ 4

Author(s):

Toshiyuki Kaji ◽

Syouichi Hiraga ◽

Noriyasu Fujii ◽

Chika Yamamoto ◽

Michiko Sakamoto ◽

...

Keyword(s):

Endothelial Cell ◽

Heparan Sulfate ◽

Cell Layer ◽

Vascular Endothelial Cell ◽

Vascular Endothelial ◽

Endothelial Cell Layer

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Endothelial Cell Layer Subjected to Impinging Flow Mimicking the Apex of an Arterial Bifurcation

Annals of Biomedical Engineering ◽

10.1007/s10439-008-9540-x ◽

2008 ◽

Vol 36 (10) ◽

pp. 1681-1689 ◽

Cited By ~ 59

Author(s):

Michael P. Szymanski ◽

Eleni Metaxa ◽

Hui Meng ◽

John Kolega

Keyword(s):

Endothelial Cell ◽

Cell Layer ◽

Arterial Bifurcation ◽

Endothelial Cell Layer ◽

Impinging Flow

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A study of rat intracerebral arterioles: methods, morphology, and reactivity

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1982.243.4.h598 ◽

1982 ◽

Vol 243 (4) ◽

pp. H598-H606 ◽

Cited By ~ 12

Author(s):

R. G. Dacey ◽

B. R. Duling

Keyword(s):

Smooth Muscle ◽

Endothelial Cell ◽

Smooth Muscle Cell ◽

Muscle Cell ◽

Vessel Wall ◽

Cell Layer ◽

Muscle Tone ◽

Vessel Diameter ◽

Cell Nuclei

Penetrating, intracerebral arterioles from rat were isolated, cannulated, and studied in vitro. Vessel wall elements were found to consist of an endothelial cell layer, one smooth muscle cell layer, and a thin adventitial layer or leptomeningeal sheath. Smooth muscle cell nuclei were oriented perpendicular to the vessel's longitudinal axis; endothelial cell nuclei were parallel to the axis. Mean vessel diameter with the smooth muscle inactivated (passive diameter) was 36.7 +/- 1.6 (SE) micrometer. Spontaneous smooth muscle tone developed at 37 degrees C and reduced vessel diameter to 70 +/- 4% of passive diameter. Vessels were activated by the extraluminal application of 140 mM KCl solution at pH 8.00, which produced a transient contraction that decayed within 30 s to a steady contraction of somewhat less intensity. Changes in intravascular pressure were used to alter wall tension of the vessels. Tension in the vessel wall was computed, and length-tension curves for the arteriolar smooth muscle were approximated. Length-tension relationships similar to those seen in other smooth-muscle preparations were found with maximal estimated force development of 1.29 x 10(-5) N . m-2. Alterations of bath pH caused changes in vessel diameter that were inversely related to extraluminal pH and varied by approximately 77% in the range from pH 6.85 to 8.00. Adenosine dilated vessels to 140 +/- 6% of control diameter at a concentration of 10(-5) M. The mechanical characteristics and the reactivity to H+, K+, and adenosine of these vessels were quantitatively consistent with in vitro data from larger cerebral vessels and in vivo data from pial arteries.

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