scholarly journals EXPRESSION OF ANTIVIRAL GENE ON TIGER SHRIMP Penaeus monodon AT DIFFERENT TISSUE AND BODY SIZE

2012 ◽  
Vol 7 (2) ◽  
pp. 95
Author(s):  
Andi Parenrengi ◽  
Andi Tenriulo ◽  
Samuel Lante
Keyword(s):  
Gene Expression ◽  
Defense Mechanism ◽  
Rna Extraction ◽  
Penaeus Monodon ◽  
Specific Response ◽  
Tiger Shrimp ◽  
Phenotype Distribution ◽  
Organ Tissue ◽  
Antiviral Gene

The role of tiger shrimp defense against invading pathogen on molecular level such antiviral gene expression is limited to be reported. Gene expression is a process which codes information of genes that is converted to the protein as a phenotype. Distribution of PmAV antivirus gene, that has been reported as an important gene on non-specific response immune, is needed to be observed to several organs/tissues and size of tiger shrimp. The aim of this study is to determine the distribution of gene antiviral expression at several organ/tissue and size of shrimp. The organs/tissues observed in this study were: gill, hepatopancres, muscle tissue, eyes, heart, stomach, gonad, and intestine. While the size of shrimp consisted of three groups, those are: (A) 10-20 g/ind., (B) 30-40 g/ind., and (C) 60-70 g/ind. Analysis of antiviral gene expression was performed by RNA extraction, followed by the cDNA syntesis, and amplification of gene expression by semi-quantitative PCR. The result of PCR optimation showed the optimal concentration of cDNA and primer was 1 μL and 50 mol, respectively for PCR final volume of 25 μL. Antiviral gene was expressed on the hepatopancreas and stomach in percentage of 50.0% and 16.7%, respectively. While the highest percentage of individual expressing the antiviral gene was observed in the shrimp size of C (66.7%), followed by B (50.0%) and A (16.7%). The result of study implied that the hepatopancreas has importantly involed in tiger shrimp defense mechanism on viral infection.

scholarly journals Enhancement of Tiger Shrimp Penaeus monodon Resistance to White Spot Syndrome Virus by Overexpression of Antiviral Gene

2021 ◽  
Vol 25 (02) ◽  
pp. 277-284
Author(s):  
Andi Parenrengi
Keyword(s):  
Gene Expression ◽  
Survival Rate ◽  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
White Spot ◽  
Tiger Shrimp ◽  
Significant Difference ◽  
White Spot Syndrome ◽  
Antiviral Gene

White spot syndrome virus (WSSV) is a significant cause of tiger shrimp mortality, and has a profound economic impact on commercial shrimp farming. A transgenic technology provides an effective method to control the viral disease by increasing the shrimp resistance. The study aimed to assess the overexpression of the PmAV (Penaeus monodon antiviral) gene on the embryo/larvae of tiger shrimp and the performance of transgenic shrimp by WSSV-challenge test. Transfection of pProAV-PmAV gene construct into the embryo was conducted using jetPEI reagent. Transient antiviral gene expression was detected at 12, 18, 24 and 30 h post transfection (hpt). The tiger shrimp larvae of PL-25 were challenged by waterborne infection technique. Survival and expression of antiviral genes were observed after challenge test. The results showed that the PmAV gene and the jetPEI reagent did not have the toxicity to the tiger shrimp larvae. The transient antiviral gene expression was exhibited at 12 hpt until the peak reached within 24 hpt and then decreased slightly at 30 hpt. This suggested that the expression of PmAV during infection was an up-regulated sign. The challenge test result showed that the transgenic tiger shrimp exhibited higher resistance (P < 0.05) to the WSSV infection (95.6% survival rate) than the control shrimp (71.1% survival rate). The 47-day transgenic tiger shrimp did not show significant difference (P > 0.05) in body weight and length gain as well as appearance, morphology, and activity from normal control shrimps. © 2021 Friends Science Publishers

The possible role of penaeidin5 from the black tiger shrimp, Penaeus monodon, in protection against viral infection

2011 ◽  
Vol 35 (5) ◽  
pp. 530-536 ◽  
Author(s):  
Noppawan Woramongkolchai ◽  
Premruethai Supungul ◽  
Anchalee Tassanakajon
Keyword(s):  
Viral Infection ◽  
Penaeus Monodon ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp

The role of MMP genes in recurrent depressive disorders and cognitive functions

2016 ◽  
Vol 28 (4) ◽  
pp. 221-231 ◽  
Author(s):  
Kinga Bobińska ◽  
Janusz Szemraj ◽  
Piotr Gałecki ◽  
Monika Talarowska
Keyword(s):  
Gene Expression ◽  
Cognitive Functions ◽  
Protein Level ◽  
Verbal Fluency ◽  
Verbal Learning ◽  
Rna Extraction ◽  
Human Organism ◽  
Enzyme Linked Immunosorbent Assay ◽  
Separate Analysis

ObjectiveAmong the 28 metalloproteinases described so far, 23 can be found in the human organism, but only few are expressed in the human brain. The main objective of this study was to analyse the relationship between MMP-2, MMP-9 and TIMP-2 gene expression and cognitive performance.MethodsThe study comprised 234 subjects: patients suffering from recurrent depressive disorder (rDD, n=139) and healthy subjects (HS, n=95). The cognitive function assessment was carried out with the help of the following tests: Trail Making Test, The Stroop Test, Verbal Fluency Test and Auditory Verbal Learning Test. Gene expression on the mRNA and protein level was evaluated for MMP-2, MMP-9 and TIMP-2 in both groups using RNA extraction, reverse transcription and enzyme-linked immunosorbent assay.ResultsBoth mRNA and protein expression levels of all the genes were significantly lower in rDD subjects as compared with HS. Having analysed the entire experimental group (N=234), significant interrelations were found between the expression of the analysed genes and the results of the tests used to measure cognitive functions. Increased expression on both the mRNA and the protein level was associated in each case with better performance of all the tests conducted. After carrying out a separate analysis on the people from the rDD group and the HS group, similar dependencies were still observed.ConclusionsThe results of our study show decreased expression of MMP-2, MMP-9 and TIMP-2 genes on both mRNA and protein levels in depression. Elevated expression of MMP-2, MMP-9, TIMP-2 positively affects cognitive efficiency: working memory, executive functions, attention functions, direct and delayed auditory–verbal memory, the effectiveness of learning processes and verbal fluency. The study highlights the important role of peripheral matrix metalloproteinases genes in depression and cognitive functions.

Role of vitelline envelope during fertilization in the black tiger shrimp, Penaeus monodon

2012 ◽  
Vol 114 (7) ◽  
pp. 659-664 ◽  
Author(s):  
Piyada Ngernsoungnern ◽  
Apichart Ngernsoungnern ◽  
Yupaporn Chaiseha ◽  
Prapee Sretarugsa
Keyword(s):  
Penaeus Monodon ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp ◽  
Vitelline Envelope

scholarly journals Multi-omics analysis to examine microbiota, host gene expression and metabolites in the intestine of black tiger shrimp (Penaeus monodon) with different growth performance

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9646
Author(s):  
Tanaporn Uengwetwanit ◽  
Umaporn Uawisetwathana ◽  
Sopacha Arayamethakorn ◽  
Juthatip Khudet ◽  
Sage Chaiyapechara ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Performance ◽  
Penaeus Monodon ◽  
Intestinal Bacteria ◽  
Average Weight ◽  
Weight Group ◽  
Phenyllactic Acid ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp ◽  
Weight Groups

Understanding the correlation between shrimp growth and their intestinal bacteria would be necessary to optimize animal’s growth performance. Here, we compared the bacterial profiles along with the shrimp’s gene expression responses and metabolites in the intestines between the Top and the Bottom weight groups. Black tiger shrimp (Penaeus monodon) were collected from the same population and rearing environments. The two weight groups, the Top-weight group with an average weight of 36.82 ± 0.41 g and the Bottom-weight group with an average weight of 17.80 ± 11.81 g, were selected. Intestines were aseptically collected and subjected to microbiota, transcriptomic and metabolomic profile analyses. The weighted-principal coordinates analysis (PCoA) based on UniFrac distances showed similar bacterial profiles between the two groups, suggesting similar relative composition of the overall bacterial community structures. This observed similarity was likely due to the fact that shrimp were from the same genetic background and reared under the same habitat and diets. On the other hand, the unweighted-distance matrix revealed that the bacterial profiles associated in intestines of the Top-weight group were clustered distinctly from those of the Bottom-weight shrimp, suggesting that some unique non-dominant bacterial genera were found associated with either group. The key bacterial members associated to the Top-weight shrimp were mostly from Firmicutes (Brevibacillus and Fusibacter) and Bacteroidetes (Spongiimonas), both of which were found in significantly higher abundance than those of the Bottom-weight shrimp. Transcriptomic profile of shrimp intestines found significant upregulation of genes mostly involved in nutrient metabolisms and energy storage in the Top-weight shrimp. In addition to significantly expressed metabolic-related genes, the Bottom-weight shrimp also showed significant upregulation of stress and immune-related genes, suggesting that these pathways might contribute to different degrees of shrimp growth performance. A non-targeted metabolome analysis from shrimp intestines revealed different metabolic responsive patterns, in which the Top-weight shrimp contained significantly higher levels of short chain fatty acids, lipids and organic compounds than the Bottom-weight shrimp. The identified metabolites included those that were known to be produced by intestinal bacteria such as butyric acid, 4-indolecarbaldehyde and L-3-phenyllactic acid as well as those produced by shrimp such as acyl-carnitines and lysophosphatidylcholine. The functions of these metabolites were related to nutrient absorption and metabolisms. Our findings provide the first report utilizing multi-omics integration approach to investigate microbiota, metabolic and transcriptomics profiles of the host shrimp and their potential roles and relationship to shrimp growth performance.

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