scholarly journals AMMONIA-REMOVAL EFFICIENCY OF A NOVEL ACINETOBACTER CALCOACETICUS STRAIN ISOLATED FROM INDUSTRIAL WASTEWATER OF NINH BINH COAL NITROGENOUS FERTILIZER PLANT

2018 ◽  
Vol 56 (2) ◽  
pp. 216
Author(s):  
Do Bien Cuong ◽  
Hoang Ngoc Han ◽  
Pham Van Thiem
Keyword(s):  
Acinetobacter Calcoaceticus ◽  
Ammonia Removal ◽  
16S Rrna Sequence ◽  
Ammonium Removal ◽  
Nitrogenous Fertilizer ◽  
Isolation And Characterization ◽  
Solid Media ◽  
Novel Bacterium ◽  
Industrial Zones

Ammonium removal from wastewaters has recently became a major concern of fertilizer manufacturers and industrial zones in Vietnam. Using aerobic ammonium remover may be a appreciate solution for reduction of treatment cost. This study describes the isolation and characterization of a novel bacterium for ammonium removal under aerobic conditions. Twelve ammonium remover strains were isolated from wastewater of a local fertilizer industry. The isolated strains were initially screened using solid media for their nitrifying activities. Among them two of the bacteria displayed the highest removal of ammonium without much accumulation of nitrite and nitrate. The isolates were identified as Acinetobacter based on biochemical characteristis and 16S rRNA sequence. One of these two isolates, Acinetobacter calcoaceticus A8 strain, showed 88% ammonium removal from industrial fertilizer wastewater.

Isolation and characterization of phosphate solubilizing bacteria from the rhizospheric soil of Ponthenpuzha forest, Pathanamthitta (District), Kerala

2021 ◽  
Vol 16 (8) ◽  
pp. 110-117
Author(s):  
Kannan Abhirami ◽  
K. Jayakumar
Keyword(s):  
Bacterial Isolate ◽  
Phosphate Solubilizing Bacteria ◽  
Cymbopogon Citratus ◽  
16S Rrna Sequence ◽  
Isolation And Characterization ◽  
16S Rrna Sequence Analysis ◽  
Phosphate Solubilizing ◽  
Level Identification

Phosphorous is considered as a major parameter for crop yield. Its availability to plant is independent of its abundance. For the plants to utilize phosphorous, it is to be converted to absorbable form. Here, the part rendered by phosphate solubilizing bacteria is significant for it plays a crucial role in the formation of plant usable phosphate from organic forms. In the present work, an effort had been made to isolate and identify phosphate solubilising bacterial isolate from the rhizhospheric soils of various plants in Ponthenpuzha forest. One of the isolate from Cymbopogon citrates responded positively to Pikovskaya’s medium by producing a halo zone during in vitro culture. Colony features and 16S rRNA sequence analysis identified the isolate as Burkholderia sps. We have reported the presence of genus Burkholderia in the rhizospheric zone of Cymbopogon citratus. Further studies are warranted for species level identification of the isolate.

ISOLATION AND CHARACTERIZATION OF BIOTECHNOLOGY RELEVANT BACTERIA FROM MARINE ENVIRONMENT

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Suganthi Thevarajoo ◽  
Chitra Selvaratnam ◽  
Kian Mau Goh ◽  
Fazilah Abd. Manan ◽  
Zaharah Ibrahim ◽  
...  
Keyword(s):  
16S Rrna ◽  
Marine Environment ◽  
Antibiotic Sensitivity ◽  
Marine Microorganisms ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
16S Rrna Sequence ◽  
Gram Staining ◽  
Isolation And Characterization

Marine environment remained as largely unexplored source for researchers to discover marine microorganisms with novel properties. This study aims to isolate marine bacteria from the seashore of Desaru, Malaysia. Totally, six bacterial strains were successfully obtained and were identified by complete 16S rRNA sequencing. The characterizations of bacterial strains were performed based on morphological tests, Gram-staining, biochemical tests, and antibiotic sensitivity. The 16S rRNA sequence of D-2, D-4, D-7, D-15, D-31, and D-33 revealed a high identity of 97 to 99% with taxa belong to genera of Pseudomonas, Marinomonas, Exiquobacterium, Micrococcus, Pseudoalteromonas, and Shewanella respectively. Strain D-31 exhibited higher tolerance towards antibiotics kanamycin, ampicillin, and erythromycin while the growth of other strains were retarded by at least two of these antibiotics. We further characterized strain D-4 and D-31 that belonged to Marinomonas sp. and Pseudoalteromonas sp.. Both genera are interesting as earlier researchers have discovered new antibacterial substances, industrial enzymes and unique secondary metabolites.

scholarly journals Isolation and Characterization of Cellulase Producing Bacteria for the Purpose of Converting Spent Mushroom Edible Canna-Waste into Organic Fertilizer  

2021 ◽  
Vol 31 (3) ◽  
pp. 12-19
Keyword(s):  
Organic Fertilizer ◽  
Quality Criteria ◽  
Bacterial Strains ◽  
16S Rrna Sequence ◽  
Production Chain ◽  
Cellulolytic Microorganisms ◽  
Isolation And Characterization ◽  
Indole 3 Acetic Acid ◽  
Halo Zone

Converting spent mushroom substrates into organic fertilizer helps to tackle the problem of pollution in edible canna starch processing villages and adds new value to the production chain of edible canna. To successfully turn the spent substrates into compost, there is certainly an indispensable role for cellulolytic microorganisms, in which Bacillus strains are always important. Several bacterial strains have been isolated from spent edible canna substrate after cultivation of monkey head mushroom in this study. Among isolated strains, the strain NDK5 has been selected exhibiting the highest cellulolytic activities with solubilization indexes of 6.14 and 18.3 mm for the ratio between the halo zone diameters and the colony diameters in the point cultivation method (SIratio) and the offset between the halo zone diameters and the agar hole diameters (SIoffset), respectively. The highest CMCase activity was 4.29 ± 0.071 U/ml. Morphological, physiological, biochemical, and 16S rRNA sequence analyses (100% homology with B. amyloliquefaciens sp. plantarum FZB42) were further carried out for the selected strain, leading to the identification of the strain as B. amyloliquefaciens sp. plantarum NDK5 strain. In addition, NDK5 was proved to have a capacity for synthesizing indole-3-acetic acid, a plant growth hormone, on an L-tryptophan-containing medium. Trial incubation of spent mushroom edible canna-substrate with the strain NDK5 showed increases in several quality criteria of the waste after 20 days of incubation, that meet the standard criteria for bio-organic fertilizer according to TCVN 7185:2002.

scholarly journals ISOLATION AND CHARACTERIZATION OF TEMPERATURE-SENSITIVE RESPIRATORY MUTANTS OF NEUROSPORA CRASSA

Genetics ◽  
1979 ◽  
Vol 93 (3) ◽  
pp. 539-555
Author(s):  
Thad H Pittenger ◽  
David J West
Keyword(s):  
Wide Spectrum ◽  
Temperature Sensitive ◽  
Isolation And Characterization ◽  
Solid Media ◽  
Filtration Enrichment ◽  
Mutant Isolation ◽  
Salicyl Hydroxamic Acid ◽  
Salicyl Hydroxamic ◽  
Considerable Resource

ABSTRACT Filtration-enrichment and inositol-less death methods of mutant isolation, coupled with a screen for cyanide-insensitive respiration, proved to be highly efficient methods for isolating temperature-sensitive (ts) nuclear Neurospora mutants having defective respiration. Eighteen different ts respiratory mutants have been isolated. Most of them are pleiotropic and defective in one or more of the following phenotypes: cytochrome aa3, b, and c (individual or multiple defects); oligomycin inhibition of ATPase activity; respiration and its inhibition by KCN and salicyl hydroxamic acid; and growth rates in liquid and solid media at 25° and 38°. Among these mutants are the first cytochrome c mutant of Neurospora and an extranuclear ts ATPase mutant. An added bonus was the fact that aver half of the mutants were affected either in ribosome assembly or in protein synthesis in the mitochondrion. We have yet to find any mutants completely lacking activities associated with the respiratory chain. However, the wide spectrum of mutants isolated here, along with those currently available, constitutes a considerable resource for investigating respiration in obligate aerobes.

scholarly journals Isolation and Characterization of a Novel Bacterium Burkholderia gladioli Bsp-1 Producing Alkaline Lipase

2019 ◽  
Vol 29 (7) ◽  
pp. 1043-1052
Author(s):  
Jing Zhu ◽  
Yanjing Liu ◽  
Yan Qin ◽  
Lixia Pan ◽  
Yi Li ◽  
...  
Keyword(s):  
Alkaline Lipase ◽  
Isolation And Characterization ◽  
Burkholderia Gladioli ◽  
Novel Bacterium

scholarly journals Isolation and characterization of Reyranella massiliensis gen. nov., sp. nov. from freshwater samples by using an amoeba co-culture procedure

2011 ◽  
Vol 61 (9) ◽  
pp. 2151-2154 ◽  
Author(s):  
Isabelle Pagnier ◽  
Didier Raoult ◽  
Bernard La Scola
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Phenotypic Characterization ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Isolation And Characterization ◽  
Novel Bacterium ◽  
Gene Sequence Analysis ◽  
Culture Procedure

The analysis of three water samples from two cooling towers and one river allowed us to isolate three strains of a novel species of the class Alphaproteobacteria which is phylogenetically related to uncultured alphaproteobacteria. Based upon 16S rRNA gene sequence analysis and phenotypic characterization, we propose to name this novel species Reyranella massiliensis gen. nov., sp. nov., type strain 521T ( = CSUR P115T  = DSM 23428T). The most closely related cultivable micro-organism to this novel bacterium is a member of the genus Magnetospirillum.

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