scholarly journals Corrective effects of original bioflavonoid complex in the cyclophosphamide-induced immunity disorders

2021 ◽  
Vol 22 (6) ◽  
pp. 1111-1120
Author(s):  
I. A. Goldina ◽  
E. V. Markova ◽  
I. A. Orlovskaya ◽  
L. B. Toporkova ◽  
V. A. Kozlov
Keyword(s):  
Bone Marrow ◽  
Immune Response ◽  
Cellular Immune Response ◽  
Proliferative Activity ◽  
Lymphoid Organs ◽  
Delayed Type Hypersensitivity ◽  
Relative Mass ◽  
Hematopoietic Progenitors ◽  
Hematopoietic Stem ◽  
Cellular Immune

Our aim was to evaluate immunomodulatory properties of an original bioflavonoid complex in experimental immune disturbances induced by cyclophosphamide (Cy). We have studied morphometric indexes of thymus and spleen, as well as blood leukocyte counts, cell proliferative activity in lymphoid organs, delayed hypersensitivity responses to T cell-dependent antigen, along with differentiation activity of bone marrow stem cells in experimental animals during Cy-induced immune suppression after a course of bioflavonoid treatment. Suspension of the bioflafonoid complex was introduced to the male mice (СВАхC57Bl/6)F1 aged 12- 14 weeks at a daily dose of 2 mg/animal (80 mg/kg), per os, using gastric catheter, over 14 days. Cytostatic immunosuppression was produced by a single intraperitoneal Cy injection. Proliferative activity of spleen and thymic cells was determined by standard method with Н3 -thymidine incorporation in the 72-h cell culture. Cellular immune response was assayed by the degree of delayed-type hypersensitivity development in response to sheep erythrocytes. The number of hematopoietic progenitors was evaluated by culturing bone marrow cells in methylcellulose-based medium. The experiments have shown mitigation of immunosuppressive effects induced by Cy, in the course of bioflavonoid complex treatment, with respect to absolute and relative mass of lymphoid organs and leukocyte numbers in peripheral blood. Moreover, we have demonstrated decreased effects of Cy treatment upon the spontaneous activity of spleen cells, mitogen-induced thymocyte and splenocyte proliferation, intensivity of delayed-type hypersensitivity response that reached the values of intact animals. Following the course of bioflavonoids, we have revealed an increase in early hematopoietic progenitors. Alleviation of Cy-induced suppressive effects upon cellular immune response, proliferation rates of immune cells, as well as stimulation of hematopoietic stem cell functions suggest a sufficient capacity of the original bioflavonoid complex for modulation of immunity and hematopoiesis, thus presenting experimental proofs for its potential usage as an adjuvant treatment of the patients with malignant diseases.

Effect of Hypertonic Saline Infusion on Postoperative Cellular Immune Function

2004 ◽  
Vol 100 (5) ◽  
pp. 1108-1118 ◽  
Author(s):  
Jens A. Kølsen-Petersen ◽  
Jens-Ole D. Nielsen ◽  
Else M. Tonnesen
Keyword(s):  
Immune Response ◽  
Immune Function ◽  
Hypertonic Saline ◽  
Cellular Immune Response ◽  
Killer Cell ◽  
Cell Activity ◽  
Abdominal Hysterectomy ◽  
Delayed Type Hypersensitivity ◽  
Laboratory Animals ◽  
Cellular Immune

Background Previous studies found hypertonicity to affect immune responses in intact laboratory animals and in human blood cell cultures. In this study, the authors investigated the cellular immune response to surgery after preoperative infusion of hypertonic saline in humans. Methods Sixty-two women scheduled to undergo abdominal hysterectomy were randomly assigned to single-blinded infusion of 4 ml/kg NaCl, 7.5%; 4 ml/kg NaCl, 0.9%; or 32 ml/kg NaCl, 0.9%, over 20 min. Blood was collected at baseline, during surgery, and 1, 24, and 48 h after surgery for the determination of leukocyte and differential counts, flow cytometric phenotyping of mononuclear cells, and natural killer cell activity against K 562 tumor cells. Phytohemagglutinin-induced lymphocyte proliferation, plasma elastase, and neutrophil chemotaxis were measured at the same time points except during surgery. The authors tested cell-mediated immune function in vivo by delayed-type hypersensitivity reaction in the skin. Results Surgery induced well-known changes in the cellular immune response, which were unrelated to the tonicity or volume of the infused fluids. Conclusion Infusion of a clinically relevant dose of hypertonic saline did not seem to modify the postoperative cellular immune response after elective abdominal hysterectomy.

scholarly journals Immunomodulating properties of the probiotic preparation, VedaBiotic in aging

2021 ◽  
pp. 41-54
Author(s):  
Е.V. Markova ◽  
◽  
О.V. Sorokin ◽  
I.А. Goldina ◽  
I.V. Savkin ◽  
...  
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Nutritional Supplement ◽  
Progressive Increase ◽  
Delayed Type Hypersensitivity ◽  
Elderly Persons ◽  
Cytostatic Treatment ◽  
Freeze Dried ◽  
Age Dependent ◽  
Cellular Immune

The homeostatic immunological dialogue between the host and its microbiota is critical to maintaining health. The weakening of the organism occurring with age, its greater dependence on the external life conditions, make old age the period of the greatest morbidity. In the context of a progressive increase in life expectancy, more and more attention is being paid to the possibility of influencing human health through personalized nutritional and therapeutic strategies aimed at modifying the intestinal microbiota, which include the use of pro- and prebiotics. The aim of this work is to study the effect of the VedaBiotic preparation, which is an original complex of probiotics (freeze-dried biomass of live active bifidobacteria, lactobacilli, Streptococcus thermophilus), prebiotics (microcrystalline cellulose, lactulose) and vitamin C, on the cellular immune response and functional activity of immunocytes in age-dependent mammals (male mice at the age of 10 months). It was found that in age-dependent mammals, a course administration of the drug leads to the stimulation of the proliferative activity of immunocytes and the cellular immune response, also including the case of the induced immunosuppression (when the delayed-type hypersensitivity reaction increased to the level observed in intact animals of similar age). The VedaBiotic also has a modulating effect on the cytokines production, causing a decrease in the pro-inflammatory cytokines IL-1β and IFN-γ, and an increase in the anti-inflammatory cytokine IL-10. Thus, the VedaBiotic preparation has a favorable immunomodulatory effect and can be used as a nutritional supplement — as a source of probiotic microorganisms — to reduce the negative side effects of cytostatic treatment, as well as by elderly persons to improve the quality of life and to prevent the development of age-associated pathology.

scholarly journals TURMERIC EXTRACT IN CORRECTION OF NERVOUS AND IMMUNE SYSTEMS FUNCTIONAL ACTIVITY PARAMETERS IN EXPERIMENTAL ALCOHOLISM

2019 ◽  
Vol 19 (1S) ◽  
pp. 215-217
Author(s):  
E V Markova ◽  
I A Goldina ◽  
B G Goldin ◽  
M A Knyazheva ◽  
I V Savkin
Keyword(s):  
Immune Response ◽  
Functional Activity ◽  
Cellular Immune Response ◽  
Experimental Models ◽  
Ethanol Solution ◽  
Delayed Type Hypersensitivity ◽  
Experimental Alcoholism ◽  
Immune Systems ◽  
Turmeric Extract ◽  
Cellular Immune

The purpose of this work was to study the effect of turmeric extract on behavior indicators, the severity of the cellular immune response in animals in a state of experimental alcoholism. Experimental models: mouse males (CBAxC57Bl/6)F1 three months of age (n = 60). Alcohol dependence in experimental animals was formed by the method of 6-month soldering with a 10% ethanol solution. In the control groups, the animals received per os water or 10% ethanol solution, in the experimental group - an extract of turmeric powder in a solution of ethanol. Mice behavior was assessed in the “open field” test. The severity of the cellular immune response to sheep erythrocytes was assessed by the intensity of the development of a delayed-type hypersensitivity reaction.It was found that the use of turmeric extract against the background of taking ethanol solution in animals with experimental alcoholism leads to the stimulation of behavior and the increase of the cellular immune response to the level characteristic of healthy animals of the corresponding age.Results indicates the protective effect of turmeric on a number of parameters of the functional activity of the nervous and immune systems during chronic ethanol intoxication.

scholarly journals Protective effect of the maternally derived porcine circovirus type 2 (PCV2)-specific cellular immune response in piglets by dam vaccination against PCV2 challenge

2012 ◽  
Vol 93 (7) ◽  
pp. 1556-1562 ◽  
Author(s):  
Yeonsu Oh ◽  
Hwi Won Seo ◽  
Kiwon Han ◽  
Changhoon Park ◽  
Chanhee Chae
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Delayed Type Hypersensitivity ◽  
Porcine Circovirus ◽  
Newborn Piglets ◽  
Specific Cellular Immune Response ◽  
Cellular Immune

The objective of the present study was to evaluate (i) the passive transfer of maternally derived functional porcine circovirus type 2 (PCV2)-specific lymphocytes of seronegative sows immunized with the PCV2 vaccine to newborn piglets and (ii) the functional role of the maternally derived PCV2-specific cellular immune response in protecting newborn piglets from challenge with PCV2. After ingesting colostrums, piglets from vaccinated sows (PT01 and PT02) have significantly higher numbers of PCV2-specific gamma interferon-secreting cells, an increased PCV2-specific delayed type hypersensitivity response, and a stronger proliferative response of peripheral blood mononuclear cells compared with piglets from non-vaccinated seronegative sows (PT03 and PT04). In the PCV2 challenge study, the number of serum genomic PCV2 copies was significantly less in piglets from vaccinated sows (PT02) compared with piglets from non-vaccinated sows (PT04) at 7–28 days post-inoculation (P<0.05 and P<0.001). The histopathological lesions and immunohistochemical scores were significantly lower in piglets of vaccinated sows compared with those of non-vaccinated sows. To our knowledge, this is the first report of transferring a maternally derived PCV2-specific cellular immune response from vaccinated dams to their offspring. Maternally derived adaptive cellular immune responses play a critical role in protecting newborn piglets challenged with PCV2 at 3 weeks of age.

The prevalence of immediate and delayed type hypersensitivity reactions to Microsporum canis antigens in cats

2003 ◽  
Vol 5 (3) ◽  
pp. 161-166 ◽  
Author(s):  
KA Moriello ◽  
DJ DeBoer ◽  
J Greek ◽  
K Kuhl ◽  
M Fintelman
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Spontaneous Recovery ◽  
Delayed Type Hypersensitivity ◽  
Microsporum Canis ◽  
Hypersensitivity Reactions ◽  
Intradermal Testing ◽  
Cellular Immune ◽  
Culture Positive ◽  
Culture Negative

Spontaneous recovery from Microsporum canis infections in cats is thought to be dependent on the development of a competent immune response. The purpose of this study was to determine the prevalence of positive delayed type hypersensitivity reactions in cats with and without dermatophytosis. Four groups of cats were intradermally skin tested with M canis extract and test sites were evaluated both subjectively and objectively at 0, 24 and 48 h after injection. Delayed intradermal testing (IDT) reactions were absent in cats not exposed to dermatophytosis ( n=20); infected–recovered cats ( n=38 culture negative lesion negative and n=43 lesion negative but culture positive) had significantly larger IDT reactions than unexposed cats and cats that were still actively infected ( n=18). Based on the results of this study, IDT with M canis extract can be used to assess the cellular immune response of cats with dermatophytosis.

scholarly journals Evaluation of Proteus vulgarisfimbriae antigen by delayed type hypersensitivity (DTH)-skin test in rabbits

2011 ◽  
Vol 35 (1) ◽  
pp. 100-106
Author(s):  
Ekram Abbas Abood Al-Samarrae
Keyword(s):  
Immune Response ◽  
Skin Test ◽  
Cellular Immune Response ◽  
Control Site ◽  
Delayed Type Hypersensitivity ◽  
Proteus Vulgaris ◽  
Significant Difference ◽  
Cellular Immune ◽  
And Control

The aim of this study is to evaluate the use of fimbriae antigens for immunization of rabbits against Proteus vulgaris bacteria results showed a higher significant difference (P<0.05) in erythema diameters in the immunized groups in compared with the control. There was no significant difference between both immunized groups 200 μg/ml and 100 μg/ml after 24 hours. Also, a higher significant differences (P<0.05) in the diameter of induration is recorded in both immunized groups compared to control site, a higher significant differences (P<0.05) in the immunized group (100 μg/ml) at the concentration 200 μg/ml compared with 100 and 50 μg/ml after 24 hours, as follow as after 48 hours except significant differences between 200 and 100 μg/ml concentration (P<0.01) and increase induration after 72 hours between both immunized groups; within groups and control site. Conclusion that the fimbrial antigen have the ability to elicit cellular immune response by delayed type hypersensitivity (DTH).

scholarly journals Mapping of the T-Cell Epitope in the Major 43-Kilodalton Glycoprotein of Paracoccidioides brasiliensisWhich Induces a Th-1 Response Protective against Fungal Infection in BALB/c Mice

1998 ◽  
Vol 66 (2) ◽  
pp. 786-793 ◽  
Author(s):  
Carlos P. Taborda ◽  
Maria A. Juliano ◽  
Rosana Puccia ◽  
Marcello Franco ◽  
Luiz R. Travassos
Keyword(s):  
Immune Response ◽  
T Cell ◽  
Cellular Immune Response ◽  
Cell Epitope ◽  
Delayed Type Hypersensitivity ◽  
Computer Assisted ◽  
T Cell Epitope ◽  
Ifn Γ ◽  
Cellular Immune ◽  
Th 1

ABSTRACT The 43-kDa glycoprotein of Paracoccidioides brasiliensis is the major diagnostic antigen of paracoccidioidomycosis, the prevalent systemic mycosis of Latin America. Apart from eliciting high antibody titers, gp43 is also immunodominant in delayed-type hypersensitivity reactions in infected animals and humans. The cellular immune response in mice to gp43 administered in complete Freund’s adjuvant involves CD4+Th-1 lymphocytes, secreting gamma interferon (IFN-γ) and interleukin 2 (IL-2) but not IL-4 and IL-10. The T-cell epitope of this antigen was mapped to a 15-amino-acid peptide (P10) based on lymphoproliferations with primed cells from three different haplotypes and on a computer-assisted protein analysis. The structural requirements of the T-cell epitope were determined by assaying a series of P10 analogous and truncated peptides. Only 12-mer or longer sequences were active, confirming presentation by major histocompatibility complex II. The HTLAIR inner core of P10 is the essential domain of the epitope, with various flanking regions possible. Immunization of mice with both gp43 and P10 led to vigorous protection against intratracheal challenge by virulent P. brasiliensis, with a >200-fold decrease in lung CFU and halting of dissemination to the spleen and liver. The protective effect of P10 is mainly attributed to an IFN-γ-mediated cellular immune response. Unlike gp43, which induces an antibody response compatible with both Th-1 and Th-2 activation in infected BALB/c mice, P10 does not induce a humoral response. Protection by gp43 and P10 was characterized by a few well-demarcated lung granulomas with numerous nonviable yeast forms or resolved lesions with no detectable fungal cells.

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