Biological Control of Soft Rot of Chinese Cabbage

A total of 91 isolates of probable antagonistic bacteria of potato soft rot bacteriumErwinia carotovorasubsp.carotovora(Ecc) were extracted from rhizospheres and endophytes of various crop plants, different soil varieties, and atmospheres in the potato farming areas of Bangladesh. Antibacterial activity of the isolated probable antagonistic bacteria was testedin vitroagainst the previously identified most common and most virulent soft rot causing bacterial strain Ecc P-138. Only two isolates E-45 and E-65 significantly inhibited thein vitrogrowth of Ecc P-138. Physiological, biochemical, and carbon source utilization tests identified isolate E-65 as a member of the genusBacillusand the isolate E-45 asLactobacillussp. The stronger antagonistic activity against Ecc P-138 was found in E-65in vitroscreening and storage potatoes. E-65 reduced the soft rot infection to 22-week storage potatoes of different varieties by 32.5–62.5% in model experiment, demonstrating its strong potential to be used as an effective biological control agent for the major pectolytic bacteria Ecc. The highest (62.5%) antagonistic effect of E-65 was observed in the Granola and the lowest (32.7%) of that was found in the Cardinal varieties of the Bangladeshi potatoes. The findings suggest that isolate E-65 could be exploited as a biocontrol agent for potato tubers.

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Kemampuan Bakteri Endofit Akar dan Ubi Kentang untuk Menekan Penyakit Busuk Lunak (Erwinia carotovora pv. carotovora) pada Ubi

Agrikultura ◽

10.24198/agrikultura.v27i3.10880 ◽

2016 ◽

Vol 27 (3) ◽

Author(s):

Noor Istifadah ◽

Muhamad Salman Umar ◽

Sudarjat Sudarjat ◽

Luciana Djaya

Keyword(s):

Biological Control ◽

Potato Tuber ◽

Endophytic Bacteria ◽

Erwinia Carotovora ◽

Soft Rot ◽

Soft Rot Disease ◽

Post Harvest ◽

Rot Disease

ABSTRACTThe abilities of endophytic bacteria from potato roots and tubers to suppress soft rot disease (Erwinia carotovora pv. carotovora) in potato tuberSoft rot disease caused by Erwinia carotovora pv. carotovora is one of limiting factors in cultivation and post harvest of potato. The eco-friendly control measure that can be developed for controlling the diseases is biological control. Microbes that are potential as biological control agents include endophytic bacteria. This paper discussed the results of study examining the potential of endophytic bacteria isolated from roots and tubers of potato to inhibit the growth of E. carotovora pv. carotovora in vitro and suppress soft rot disease in potato tuber. The results showed that among 24 isolates examined, four isolates of endophytic bacteria (one isolate from potato tuber and three isolates from potato roots) inhibited the growth of E. carotovora pv. carotovora in vitro with inhibition zone 3.5-6.8 mm. In the in vivo test, the isolates inhibited the soft rot disease in potato tuber by 71.5-86.4%. The isolate that tended to show relatively better inhibition in vitro and in vivo was isolate from potato tuber which is CK U3 (Lysinibacillus sp.)Keywords: Biological control, Endophytic bacteria, Post-harvest, Potato, Soft rot diseaseABSTRAKPenyakit busuk lunak yang disebabkan bakteri Erwinia carotovora pv. carotovora, merupakan salah satu kendala dalam budidaya dan pascapanen kentang. Cara pengendalian ramah lingkungan yang dapat dikembangkan untuk menekan penyakit tersebut adalah pengendalian biologi. Kelompok mikroba yang berpotensi sebagi agens pengendali biologi adalah bakteri endofit. Artikel ini mendiskusikan potensi isolat bakteri endofit yang berasal dari ubi dan akar kentang untuk menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dan menekan perkembangan penyakit busuk lunak pada ubi kentang. Hasil percobaan menunjukkan bahwa diantara 24 isolat bakteri yang diuji, terdapat empat isolat bakteri endofit (satu isolat dari ubi kentang dan tiga isolat dari akar kentang) yang dapat menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dengan zona penghambatan sebesar 3,5-6,8 mm. Pada pengujian secara in vivo, isolat-isolat tersebut dapat menekan perkembangan penyakit busuk lunak pada ubi kentang sebesar 71,5-86,4%. Isolat yang cenderung menunjukkan penghambatan relatif lebih baik secara in vitro dan in vivo adalah isolat bakteri endofit asal ubi kentang yaitu isolat CK U3 (Lysinibacillus sp.).Kata Kunci: Pengendalian biologi, Bakteri endofit, Pascapanen, Kentang, Penyakit busuk basah

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Biological control of the soft rot bacterium Pectobacterium carotovorum by Bacillus amyloliquefaciens strain Ar10 producing glycolipid-like compounds

Microbiological Research ◽

10.1016/j.micres.2018.08.013 ◽

2018 ◽

Vol 217 ◽

pp. 23-33 ◽

Cited By ~ 3

Author(s):

Sana Azaiez ◽

Imen Ben Slimene ◽

Ines Karkouch ◽

Rym Essid ◽

Selim Jallouli ◽

...

Keyword(s):

Biological Control ◽

Bacillus Amyloliquefaciens ◽

Soft Rot ◽

Pectobacterium Carotovorum

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Biological Control Efficacy of Bacillus Licheniformis Hg03 Against Soft Rot Disease of Postharvest Peach

SSRN Electronic Journal ◽

10.2139/ssrn.4000312 ◽

2022 ◽

Author(s):

Yunpeng Wang ◽

Xiaoli Wang ◽

Jingfeng Zhu ◽

Huan Wei ◽

Zhipeng Ding ◽

...

Keyword(s):

Biological Control ◽

Bacillus Licheniformis ◽

Soft Rot ◽

Control Efficacy ◽

Soft Rot Disease ◽

Rot Disease

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Control on Soft Rot of Chinese Cabbage Using Harpin Protein

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.183-185.2078 ◽

2011 ◽

Vol 183-185 ◽

pp. 2078-2081

Author(s):

Tian Lei Qiu ◽

Min Wang ◽

Xiao Hong Sun ◽

Mei Lin Han ◽

Xu Ming Wang

Keyword(s):

Chinese Cabbage ◽

Soft Rot ◽

Coli Strain ◽

Economic Losses ◽

Common Disease ◽

Control Effect ◽

Protein Preparation ◽

E Coli ◽

Wettable Powder ◽

Harpin Protein

Soft rot of Chinese cabbage is a common disease that causes serious damage and economic losses. In this study, the control on soft rot of growing and postharvest Chinese cabbage was carried out, using Harpin protein which was the expressed product of a recombinant E. coli strain. The experimental results indicate that Harpin protein preparation containing 3% pure protein powder and 97% wettable powder of Bacillus Thuringiensis (Bt), could effectively control soft rot of Chinese cabbage. The control effect on soft rot reached as high as 90% for growing Chinese cabbage using Harpin protein at 6-10mg/m2 of dosage, and the control effect reached approximately 75% for postharvest Chinese cabbage. Harpin protein stored for 6 months at 20-25 0C had the similarly biological activity with the newly prepared protein.

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Biological control of potato soft rot caused by Erwinia carotovora subsp. carotovora

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-018-0100-x ◽

2018 ◽

Vol 28 (1) ◽

Cited By ~ 2

Author(s):

Ehab A. Salem ◽

Yasser M. Abd El-Shafea

Keyword(s):

Biological Control ◽

Erwinia Carotovora ◽

Soft Rot

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The Iturin-like Lipopeptides Are Essential Components in the Biological Control Arsenal of Bacillus subtilis Against Bacterial Diseases of Cucurbits

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-06-11-0162 ◽

2011 ◽

Vol 24 (12) ◽

pp. 1540-1552 ◽

Cited By ~ 74

Author(s):

Houda Zeriouh ◽

Diego Romero ◽

Laura García-Gutiérrez ◽

Francisco M. Cazorla ◽

Antonio de Vicente ◽

...

Keyword(s):

Biological Control ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Xanthomonas Campestris ◽

Soft Rot ◽

Bacterial Diseases ◽

Chromatography Analysis ◽

Essential Components ◽

Bacillus Spp

The antibacterial potential of four strains of Bacillus subtilis, UMAF6614, UMAF6619, UMAF6639, and UMAF8561, previously selected on the basis of their antifungal activity and efficacy against cucurbit powdery mildew, was examined. Among these strains, UMAF6614 and UMAF6639 showed the highest antibacterial activity in vitro, especially against Xanthomonas campestris pv. cucurbitae and Pectobacterium carotovorum subsp. carotovorum. These strains produced the three families of lipopeptide antibiotics known in Bacillus spp.: surfactins, iturins, and fengycins. Using thin-layer chromatography analysis and direct bioautography, the antibacterial activity could be associated with iturin lipopeptides. This result was confirmed by mutagenesis analysis using lipopeptide-defective mutants. The antibacterial activity was practically abolished in iturin-deficient mutants, whereas the fengycin mutants retained certain inhibitory capabilities. Analyses by fluorescence and transmission electron microscopy revealed the cytotoxic effect of these compounds at the bacterial plasma membrane level. Finally, biological control assays on detached melon leaves demonstrated the ability of UMAF6614 and UMAF6639 to suppress bacterial leaf spot and soft rot; accordingly, the biocontrol activity was practically abolished in mutants deficient in iturin biosynthesis. Taken together, our results highlight the potential of these B. subtilis strains as biocontrol agents against fungal and bacterial diseases of cucurbits and the versatility of iturins as antifungal and antibacterial compounds.

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