Molecular detection of Trypanosoma sp. and Blastocrithidia sp. (Trypanosomatidae) in phlebotomine sand flies (Psychodidae) in the Federal District of Brazil

Several phlebotomine sand fly species have been regarded as putative or proven vectors of parasites of the genus Leishmania in Brazil, but data for the northeastern region remains incipient. In this study, a total of 600 phlebotomine sand flies were grouped in pools of 10 specimens each and tested by a Leishmania genus-specific PCR and by a PCR targeting Leishmania (Leishmania) infantum. Fourteen out of 60 pools were positive by the genus-specific PCR, being five pools of L. migonei, seven of L. complexa, one of L. sordellii and one of L. naftalekatzi, which correspond to a minimal infection rate of 2.3% (14/600). Our results, associated with their known anthropophily and their abundance, suggest the participation of L. migonei and L. complexa as vectors of Leishmania in northeastern Brazil. Remarkably, this is the first time in this country that the detection of Leishmania DNA in L. sordellii and L. naftalekatzi has been reported, but future studies are necessary to better understand the significance of these findings.

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Molecular Detection ofLeishmaniaDNA in Wild-Caught Phlebotomine Sand Flies (Diptera: Psychodidae) From a Cave in the State of Minas Gerais, Brazil

Journal of Medical Entomology ◽

10.1093/jme/tjw137 ◽

2016 ◽

Vol 54 (1) ◽

pp. 196-203 ◽

Cited By ~ 6

Author(s):

G.M.L. Carvalho ◽

R. P. Brazil ◽

F. D. Rêgo ◽

M.C.N.F. Ramos ◽

A.P.L.A. Zenóbio ◽

...

Keyword(s):

Molecular Detection ◽

Minas Gerais ◽

The State ◽

Sand Flies ◽

Phlebotomine Sand Flies

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Molecular Detection of Bartonella sp. in Psathyromyia shannoni and Lutzomyia cruciata From Northeastern Mexico

Frontiers in Tropical Diseases ◽

10.3389/fitd.2021.780808 ◽

2021 ◽

Vol 2 ◽

Author(s):

Yokomi N. Lozano-Sardaneta ◽

Nadia Joselyne Soto-Olguín ◽

Jorge J. Rodríguez-Rojas ◽

Sokani Sánchez-Montes ◽

Eduardo A. Rebollar-Téllez ◽

...

Keyword(s):

Dna Sequences ◽

Molecular Detection ◽

Citrate Synthase ◽

Phylogenetic Reconstruction ◽

Sand Fly ◽

Sand Flies ◽

Light Traps ◽

Bartonella Bacilliformis ◽

Phlebotomine Sand Flies ◽

Northeastern Mexico

Phlebotomine sand flies are vectors of Leishmania spp., Bartonella bacilliformis, and several arboviruses worldwide. In Mexico, the presence of Bartonella species is associated sporadically with arthropods and little is known on the diversity of insects that could be incriminated with its transmission. The aim of this study was to perform a molecular detection of Bartonella DNA in sand fly species collected in northeastern Mexico. Sand flies were collected at the states of Nuevo Leon and Tamaulipas from June to August 2010, using 16 light traps per night. Sand fly species were morphologically identified, and for Bartonella detection, we amplified ~378 bp of the citrate synthase gene (gltA). DNA sequences were compared in a phylogenetic reconstruction based on maximum likelihood. A total of 532 specimens from seven sand fly species were morphologically identified, where 11 specimens from Tamaulipas tested positive for the presence of a new lineage of Bartonella sp. associated with Psathyromyia shannoni and Lutzomyia cruciata. This work represents the second record of Bartonella-associated with sand flies outside of the endemic area of Carrion’s disease. More studies are necessary to understand their life cycle, transmission dynamics, and their relationship with sand fly species.

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Molecular detection of Leishmania DNA and identification of blood meals in wild caught phlebotomine sand flies (Diptera: Psychodidae) from southern Portugal

Parasites & Vectors ◽

10.1186/s13071-015-0787-4 ◽

2015 ◽

Vol 8 (1) ◽

Cited By ~ 34

Author(s):

Carla Maia ◽

Ricardo Parreira ◽

José Manuel Cristóvão ◽

Ferdinando Bernardino Freitas ◽

Maria Odete Afonso ◽

...

Keyword(s):

Molecular Detection ◽

Sand Flies ◽

Phlebotomine Sand Flies ◽

Southern Portugal ◽

Blood Meals

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Diversity of phlebotomine sand flies and molecular detection of trypanosomatids in Brumadinho, Minas Gerais, Brazil

PLoS ONE ◽

10.1371/journal.pone.0234445 ◽

2020 ◽

Vol 15 (6) ◽

pp. e0234445

Author(s):

Aline Tanure ◽

Felipe Dutra Rêgo ◽

Gabriel Barbosa Tonelli ◽

Aldenise Martins Campos ◽

Paloma Helena Fernandes Shimabukuro ◽

...

Keyword(s):

Molecular Detection ◽

Minas Gerais ◽

Sand Flies ◽

Phlebotomine Sand Flies

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Molecular Detection of Leishmania in Phlebotomine Sand Flies (Diptera: Psychodidae) from a Cutaneous Leishmaniasis Focus at Xakriabá Indigenous Reserve, Brazil

PLoS ONE ◽

10.1371/journal.pone.0122038 ◽

2015 ◽

Vol 10 (4) ◽

pp. e0122038 ◽

Cited By ~ 29

Author(s):

Felipe Dutra Rêgo ◽

Jeronimo Marteleto Nunes Rugani ◽

Paloma Helena Fernandes Shimabukuro ◽

Gabriel Barbosa Tonelli ◽

Patrícia Flávia Quaresma ◽

...

Keyword(s):

Cutaneous Leishmaniasis ◽

Molecular Detection ◽

Sand Flies ◽

Phlebotomine Sand Flies

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Ocorrência de flebotomíneos (Diptera: Psychodidae) em matas de galeria no Distrito Federal, Brasil

EntomoBrasilis ◽

10.12741/ebrasilis.v7i3.398 ◽

2014 ◽

Vol 7 (3) ◽

pp. 216-221 ◽

Cited By ~ 2

Author(s):

Jônatas Barbosa Cavalcante Ferreira ◽

Mariana Aragão Macedo ◽

Douglas de Almeida Rocha ◽

Tauana de Sousa Ferreira ◽

Marcos Takashi Obara ◽

...

Keyword(s):

Rainy Season ◽

Conservation Status ◽

Ground Level ◽

Federal District ◽

Dry Season ◽

Sand Fly ◽

Sand Flies ◽

Phlebotomine Sand Flies ◽

Gallery Forests ◽

Distrito Federal

O objetivo deste estudo foi analisar a ocorrência de flebotomíneos em matas de galeria do Distrito Federal (DF), em diferentes estações climáticas, estratos verticais e condições de preservação das matas. As capturas foram realizadas em três áreas na Fazenda Água Limpa da Universidade de Brasília. Em cada área foi analisada a ocorrência de flebotomíneos em 20 pontos visitados em dois meses, novembro de 2012 (estação chuvosa) e maio de 2013 (estação seca). Armadilhas luminosas HP foram instaladas a 1,5 m e a 10 m de altura do solo, operando entre 17:00 e 07:00 h, por três dias consecutivos. Foram instaladas 359 armadilhas-noite na estação chuvosa e 291 na seca. Nenhum flebotomíneo foi capturado na estação chuvosa. Na estação seca foram capturados 250 exemplares. As espécies identificadas foram Psathyromyia runoides (Fairchild & Hertig) (79,9%), Pintomyia monticola (Costa Lima) (9,7%), Micropygomyia quinquefer (Dyar) (3,7%), Psathyromyia shannoni (Dyar) (3%), Bichromomyia flaviscutellata (Mangabeira) (0,7%), Brumptomyia sp. (0,7%), Pintomyia kuscheli (Le Pont, Martinez, Torrez-Espejo & Dujardin) (0,7%), Psathyromyia lutziana (Costa Lima) (0.7%) e Sciopemyia sordellii (Shannon & Del Ponte) (0,7%). Os resultados indicam que em matas de galeria do DF há uma maior ocorrência de flebotomíneos na estação seca, no nível do solo e em áreas preservadas. Registra-se pela primeira vez Pa. runoides, Pi. kuscheli e Mi. quinquefer no DF. Occurrence of Phlebotomine Sand Flies (Diptera: Psychodidae) in the Gallery Forests of the Federal District, Brazil Abstract. We analyzed the occurrence of phlebotomine sand flies in the gallery forests of the Federal District (FD), in different seasons, forest strata and conservation status. The samples were collected in three areas in the Água Limpa Farm, University of Brasília. In each area the occurrence data were analyzed in 20 collection sites visited on two months, November 2012 (rainy season) and May 2013 (dry season). HP light traps were installed at 1.5 m and 10 m above the ground operating from 17:00 to 7:00 h, during three consecutive days. We set 359 trap-nights during the rainy season and 291 during the dry season. None sand fly was caught during the rainy season. In the dry season 250 sand flies were captured. The identified species were Psathyromyia runoides (Fairchild & Hertig) (79.9%), Pintomyia monticola (Costa Lima) (9.7%), Micropygomyia quinquefer (Dyar) (3.7%), Psathyromyia shannoni (Dyar) (3%), Bichromomyia flaviscutellata (Mangabeira) (0.7%), Brumptomyia sp. (0.7%), Pintomyia kuscheli (Le Pont, Martinez, Torrez-Espejo & Dujardin) (0.7%), Psathyromyia lutziana (Costa Lima) (0.7%), and Sciopemyia sordellii (Shannon & Del Ponte) (0.7%). The results indicated that in the gallery forests of the FD there is a higher occurrence of sand flies in the dry season, at ground level and in preserved areas. Pa runoides, Pi. kuscheli and Mi. quinquefer were recorded for the first time in the FD.

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Influence of Moonlight On Light-Emitting Diode Trap Catches of Phlebotomine Sand Flies (Diptera: Psychodidae)1

Entomological News ◽

10.3157/021.129.0105 ◽

2020 ◽

Vol 129 (1) ◽

pp. 35 ◽

Cited By ~ 1

Author(s):

Apoliana Araújo da Silva ◽

Benedita Maria Costa-Neta ◽

Francinaldo Soares Silva

Keyword(s):

Light Emitting Diode ◽

Sand Flies ◽

Phlebotomine Sand Flies ◽

Light Emitting ◽

Trap Catches

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Phlebotomine Sand Fly Fauna andLeishmaniaInfection in the Vicinity of the Serra do Cipó National Park, a Natural Brazilian Heritage Site

BioMed Research International ◽

10.1155/2015/385493 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 14

Author(s):

Rosana Silva Lana ◽

Érika Monteiro Michalsky ◽

Consuelo Latorre Fortes-Dias ◽

João Carlos França-Silva ◽

Fabiana de Oliveira Lara-Silva ◽

...

Keyword(s):

National Park ◽

Sand Fly ◽

Sand Flies ◽

Local Data ◽

Phlebotomine Sand Flies ◽

Serra Do Cipó ◽

Heritage Site ◽

One Year ◽

The One ◽

Favorable Conditions

In the New World, the leishmaniases are primarily transmitted to humans through the bites ofLeishmania-infectedLutzomyia(Diptera: Psychodidae) phlebotomine sand flies. Any or both of two basic clinical forms of these diseases are endemic to several cities in Brazil—the American cutaneous leishmaniasis (ACL) and the American visceral leishmaniasis (AVL). The present study was conducted in the urban area of a small-sized Brazilian municipality (Jaboticatubas), in which three cases of AVL and nine of ACL have been reported in the last five years. Jaboticatubas is an important tourism hub, as it includes a major part of the Serra do Cipó National Park. Currently, no local data is available on the entomological fauna or circulatingLeishmania. During the one-year period of this study, we captured 3,104 phlebotomine sand flies belonging to sixteenLutzomyiaspecies. In addition to identifying incriminated or suspected vectors of ACL with DNA of the etiological agent of AVL and vice versa, we also detectedLeishmaniaDNA in unexpectedLutzomyiaspecies. The expressive presence of vectors and naturalLeishmaniainfection indicates favorable conditions for the spreading of leishmaniases in the vicinity of the Serra do Cipó National Park.

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