Seroprevalence of Trypanosoma cruzi infection among blood donors in the state of Bahia, Brazil

Trypanosoma cruzi, the etiologic agent of Chagas disease (CD), is transmitted by hematophagous insects belonging to the subfamily Triatominae. After elimination of Triatoma infestans, the infestation of human dwellings by secondary species of vectors continues to pose a risk of transmission of the parasite. Our aim was to investigate the T. cruzi presence in triatomines and humans in rural households in the State of Paraná, southern Brazil. The capture of the insects was carried out by technicians of the municipalities after residents reported the outbreak. Five residents and 27 triatomines captured in four municipalities in the North and Midwest of the state were evaluated. The research of T. cruzi was carried out using parasitological, serological, and molecular techniques, in human blood, excreta, intestinal contents and insect macerate. Panstrongylus megistus, P. geniculatus and Triatoma sp. were identified. Ten specimens of P. megistus were captured in a house in Mandaguari with five residents and presented an infection rate of 70% for T. cruzi like. All residents tested negative for T. cruzi infection. Another 15 P. megistus were captured in the peridomicile in Janiópolis and had 100.0% positivity. The only adult specimen of P. geniculatus captured in the intradomicile in Amaporã, as well as the nymph of Triatoma in the peridomicile in Paiçandu, were negative. The finding of P. megistus naturally infected by T. cruzi in households in rural area of Paraná demonstrates a potential risk of vector transmission of CD in these regions.

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Risk factors for Trypanosoma cruzi infection in California blood donors

Transfusion ◽

10.1046/j.1537-2995.1996.36396182140.x ◽

1996 ◽

Vol 36 (3) ◽

pp. 227-231 ◽

Cited By ~ 38

Author(s):

SA Galel ◽

LV Kirchhoff

Keyword(s):

Risk Factors ◽

Trypanosoma Cruzi ◽

Blood Donors ◽

Cruzi Infection

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Characteristics of Triatomine infestation and natural Trypanosoma cruzi infection in the State of Rio Grande do Norte, Brazil

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/0037-8682-0300-2015 ◽

2016 ◽

Vol 49 (1) ◽

pp. 57-67 ◽

Cited By ~ 10

Author(s):

Andressa Noronha Barbosa-Silva ◽

Antonia Cláudia Jácome da Câmara ◽

Kiev Martins ◽

Daniela Ferreira Nunes ◽

Pedro Igor Câmara de Oliveira ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

Rio Grande ◽

The State ◽

Cruzi Infection

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The United States Trypanosoma cruzi Infection Study: evidence for vector-borne transmission of the parasite that causes Chagas disease among United States blood donors

Transfusion ◽

10.1111/j.1537-2995.2012.03581.x ◽

2012 ◽

Vol 52 (9) ◽

pp. 1922-1930 ◽

Cited By ~ 103

Author(s):

Paul T. Cantey ◽

Susan L. Stramer ◽

Rebecca L. Townsend ◽

Hany Kamel ◽

Karen Ofafa ◽

...

Keyword(s):

United States ◽

Trypanosoma Cruzi ◽

Chagas Disease ◽

Blood Donors ◽

The United States ◽

Vector Borne ◽

Cruzi Infection ◽

Infection Study

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Disagreement between PCR and serological diagnosis of Trypanosoma cruzi infection in blood donors from a Colombian endemic region

Biomédica ◽

10.7705/biomedica.5441 ◽

2021 ◽

Vol 41 (Supl. 1) ◽

pp. 47-59

Author(s):

Liliana Torcoroma García Sánchez ◽

Jhancy Rocío Aguilar Jiménez ◽

Marly Yojhana Bueno ◽

Erika Marcela Moreno Moreno ◽

Herminia Ramírez ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

Chagas Disease ◽

Blood Donors ◽

False Negative ◽

Chronic Phase ◽

Antibody Detection ◽

Low Grade ◽

Endemic Region ◽

Infected People ◽

Cruzi Infection

Introduction: Chagas’ disease is the leading cause of infectious myocarditis worldwide. This infection caused by Trypanosoma cruzi is usually life-long and asymptomatic; however, the third part of infected people can develop severe or even fatal cardiomyopathy. As the parasitemia in the chronic phase is both low-grade and intermittent, T. cruzi infection is principally detected by serology, although this method has sensitivity and specificity limitations.Objective: To determine the level of agreement between serologic and molecular tests in 658 voluntary blood donors from six provinces in the Colombian department of Santander.Materials and methods: We evaluated an array of diagnostic technologies by cross-section sampling performing a serological double diagnostic test for T. cruzi antibody detection (Chagas III ELISA™, BiosChile Group, and ARCHITECT Chagas CMIA™, Abbott), and DNA detection by polymerase chain reaction (PCR). We collected the demographic, clinical, and epidemiological information of participants. The sample size was calculated using Epidat™ and the statistical analysis was done with Stata 12.1™.Results: PCR was six times more sensitive in detecting T. cruzi infection than ELISA/CMIA with prevalence values of 1.8% (12/658) and 0.3% (2/658), respectively, and kappa=0.28 (95%CI: -0.03 - 0.59). In contrast, serology showed a sensitivity of 16.7% (95%CI: 2.09 - 48.4) and a specificity of 100% (95%CI: 99.4 - 100). All seropositive samples were found to be positive by PCR.Conclusions: The implementation of PCR as a complementary method for screening donors could reduce the probability of false negative and the consequent risk of transfusional-transmission of Chagas’ disease, especially in endemic regions.

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Risk Factors and Screening for Trypanosoma cruzi Infection of Dutch Blood Donors

PLoS ONE ◽

10.1371/journal.pone.0151038 ◽

2016 ◽

Vol 11 (3) ◽

pp. e0151038 ◽

Cited By ~ 8

Author(s):

Ed Slot ◽

Boris M. Hogema ◽

Michel Molier ◽

Aldert Bart ◽

Hans L. Zaaijer

Keyword(s):

Risk Factors ◽

Trypanosoma Cruzi ◽

Blood Donors ◽

Cruzi Infection

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Screening blood donors for Trypanosoma cruzi infection in a nonendemic area of Brazil

Transfusion ◽

10.1046/j.1537-2995.1998.38698326342.x ◽

1998 ◽

Vol 38 (6) ◽

pp. 611-612 ◽

Cited By ~ 1

Author(s):

L Barjas-Castro Mde ◽

ME Guariento ◽

CS Vincente ◽

V Castro

Keyword(s):

Trypanosoma Cruzi ◽

Blood Donors ◽

Cruzi Infection

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Seroepidemiology of Trypanosoma cruzi infection in the semiarid rural zone of the State of Rio Grande do Norte, Brazil

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86822012000300013 ◽

2012 ◽

Vol 45 (3) ◽

pp. 346-352 ◽

Cited By ~ 10

Author(s):

Carlos Ramon do Nascimento Brito ◽

George Harisson Felinto Sampaio ◽

Antonia Cláudia Jácome da Câmara ◽

Daniela Ferreira Nunes ◽

Paulo Roberto Medeiros de Azevedo ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

Educational Level ◽

Endemic Area ◽

Rio Grande ◽

The State ◽

The West ◽

Blood Samples ◽

Western Area ◽

Rural Zone ◽

Cruzi Infection

INTRODUCTION: A seroepidemiological survey was carried out to evaluate Trypanosoma cruzi infection in an endemic area of the State of Rio Grande do Norte, Brazil, involving rural residents. METHODS: Sixteen municipalities were randomly selected, 15 from the west mesoregion and one from the central, with an estimated population of 83,852 individuals. A total of 1,950 blood samples were collected in the west mesoregion and 390 in Caicó. Anti-T. cruzi antibodies were detected using the Chagatest® ELISA HAI-hemagglutination kits and indirect immunofluorescence. As sera presented indeterminate results, TESAcruzi® western blot was performed to confirm reactivity. RESULTS: An estimated seroprevalence of 6.5% was determined for the west mesoregion and 3.3% for Caicó. Seropositivity rises progressively with the age of individuals, up to 40 years in Caicó and up to 50 years in the west mesoregion. Only educational level and knowledge regarding the triatomine were associated with seropositivity. No seroreactive individuals under 18 years of age were identified. CONCLUSIONS: Infection by T. cruzi remains high and is concentrated in municipalities in the central western area of the west mesoregion; however, evidence suggests a decline in vector transmission in this mesoregion and in Caicó. Epidemiological variables appear not to influence seropositivity, with the exception of education and knowledge concerning the triatomine, among seroreactive individuals from the west mesoregion.

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