Fibroblast Viability after Storage at 20 °C in Milk, Hank's Balanced Salt Solution and Coconut Water

Abstract The objective of this study was to evaluate the effectiveness of various storage media at 20 °C in maintaining the viability of human periodontal ligament fibroblasts (HPLF) over time. HPLF were maintained at 20 °C in skim milk (SM), whole milk (WM), freshly prepared Hank's balanced salt solution (HBSS), Save-A-Tooth(r), natural coconut water (NCW), coconut water industrialized (ICW) and tap water (negative control) for 3, 6, 24, 48, 72, 96 and 120 h. Cells maintained in Minimal Essential Medium (MEM-37) at 37 °C served as a positive control. Cell viability was determined by MTT assay. Statistical analysis was performed by Kruskal-Wallis test and Scheffe test (α = 5%). From 24 h, NCW was significantly better in maintaining cell viability than all other tested storage media (p<0.05). SM and WM were significantly better than HBSS for up to 72 h. Save-A-Tooth(r) and ICW were the worst conservation storage media. In conclusion, the effectiveness of the tested storage media to maintain the viability of the periodontal ligament cells was as follows, in a descending order: NCW > MEM-37> SM and IM> HBSS> ICW > Save-A-Tooth(r)> tap water.

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Influence of Storage Media Containing Salvia officinalis on Survival of Periodontal Ligament Cells

The Journal of Contemporary Dental Practice ◽

10.5005/jcdp-9-6-17 ◽

2008 ◽

Vol 9 (6) ◽

pp. 17-24 ◽

Cited By ~ 11

Author(s):

Fatih Ozan ◽

Zübeyde Akin Polat ◽

Bektaş Tepe ◽

Kürşat Er

Keyword(s):

Cell Viability ◽

Periodontal Ligament ◽

Tap Water ◽

Salvia Officinalis ◽

The Other ◽

Periodontal Ligament Cells ◽

Storage Medium ◽

Trypan Blue Exclusion ◽

Storage Media ◽

Pdl Cells

Aim The purpose of this study was to determine the ability of Salvia officinalis (S. officinalis) extracts to serve as a storage medium for the maintenance of periodontal ligament (PDL) cell viability of avulsed teeth. Methods and Materials PDL cells were obtained from healthy third molars and cultured in Dulbecco's Modified Eagle's Medium (DMEM). Cultures were subjected to 4, 2.5, 1.5, and 0.5% S. officinalis solutions, Hank's balanced salt solution (HBSS), phosphate buffered saline (PBS), and tap water. Tissue culture plates were incubated with experimental media at 37°C for 1, 3, 6, 12 or 24 hours. PDL cell viability was assessed by trypan blue exclusion. Statistical analysis of the data was performed by one-way analysis of variance (ANOVA) complemented by the Tukey's test. The level of significance was 5% (p< 0.05). Results The results showed 2.5% S. officinalis was a more effective storage medium than the other experimental solutions (p<0.05). Only at 1 hour and 3 hours was there found similar effect between 2.5% S. officinalis and HBSS. At 24 hours, 2.5% S. officinalis was found to be significantly better than the other solutions tested. Conclusion S. officinalis can be recommended as a suitable transport medium for avulsed teeth. Clinical Significance The findings of this study support the use of S. officinalis as another option for clinicians to use to store and transport avulsed teeth until reimplantation procedures can be done. Citation Özan F, Polat ZA, Tepe B, Er K. Influence of Storage Media Containing Salvia officinalis on Survival of Periodontal Ligament Cells. J Contemp Dent Pract 2008 September; (9)6:017-024.

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Viability of human periodontal ligament fibroblasts in milk, Hank’s balanced salt solution and coconut water as storage media

International Endodontic Journal ◽

10.1111/j.1365-2591.2010.01809.x ◽

2010 ◽

Vol 44 (2) ◽

pp. 111-115 ◽

Cited By ~ 34

Author(s):

B. D. M. Souza ◽

D. D. Lückemeyer ◽

J. F. Reyes-Carmona ◽

W. T. Felippe ◽

C. M. O. Simões ◽

...

Keyword(s):

Salt Solution ◽

Periodontal Ligament ◽

Coconut Water ◽

Periodontal Ligament Fibroblasts ◽

Storage Media ◽

Human Periodontal Ligament Fibroblasts

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Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study

Revista de Odontologia da UNESP ◽

10.1590/1807-2577.09216 ◽

2016 ◽

Vol 45 (4) ◽

pp. 234-239 ◽

Cited By ~ 1

Author(s):

Priscilla Barbosa Ferreira SOARES ◽

Aletheia Moraes ROCHA ◽

Manuella Verdinelli de Paula REIS ◽

Camilla Christian Gomes MOURA ◽

Carlos José SOARES

Keyword(s):

Cell Viability ◽

Tap Water ◽

Human Fibroblasts ◽

In Vitro Study ◽

Positive Control ◽

Negative Control ◽

Coconut Water ◽

Ph Adjustment ◽

Adjustment Method

Abstract Objective This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO3), (3) Triethanolamine (C6H15NO3), (4) 2-Amino-2-Methil-1-Propanol (C4H11NO). Fibroblasts were plated at 2×104/ well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey’s and Dunnet’s test. Result There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.

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Comparative in vitro study of the effectiveness of Green tea extract and common storage media on periodontal ligament fibroblast viability

European Journal of Dentistry ◽

10.4103/1305-7456.184158 ◽

2016 ◽

Vol 10 (03) ◽

pp. 408-412 ◽

Cited By ~ 6

Author(s):

Fahimeh Adeli ◽

Ebrahim Zabihi ◽

Zeinab Abedian ◽

Samane Gharekhani ◽

Mahdi Pouramir ◽

...

Keyword(s):

Green Tea ◽

Periodontal Ligament ◽

Sucrose Solution ◽

Tap Water ◽

Green Tea Extract ◽

Periodontal Ligament Cells ◽

Tetrazolium Salt ◽

Storage Media ◽

Good Ability ◽

Tea Extract

ABSTRACT Objective: Green tea extract (GTE) was shown to be effective in preserving periodontal ligament fibroblasts (PDLFs) of avulsed teeth. This study aimed at determining the potential of GTE in preserving the viability of PDLFs comparing with different storage media. Materials and Methods: Periodontal ligament cells were obtained from freshly extracted healthy impacted third molars and cultured in Dulbecco's Modified Eagle Medium (DMEM). Cell viability was determined by storing the cells in seven media; DMEM, tap water, Hank's balanced salt solution (HBSS), whole milk, hypotonic sucrose solution, GTE, and GTE + sucrose for 1, 2, 4, and 24 h at 37°C using tetrazolium salt-based colorimetric (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) assay. Statistical analysis was performed by one-way analysis of variance and post hoc tests. Results: GTE showed significantly higher protective effect than HBSS at 2, 4, and 24 h (P = 0.009, P = 0.02, P = 0.016), DMED at 2 h (P = 0.003), and milk at 4 h (P = 0.039). Conclusion: Although with undesirable osmolality and pH, GTE had a good ability in preserving the PDLFs comparing with other studied media.

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Evaluation and comparison of efficacy of three different storage media, coconut water, propolis, and oral rehydration solution, in maintaining the viability of periodontal ligament cells

Journal of Conservative Dentistry ◽

10.4103/0972-0707.105303 ◽

2013 ◽

Vol 16 (1) ◽

pp. 71 ◽

Cited By ~ 11

Author(s):

Tulsi Sanghavi ◽

Nimisha Shah ◽

Vaishali Parekh ◽

Kiran Singbal

Keyword(s):

Periodontal Ligament ◽

Oral Rehydration Solution ◽

Coconut Water ◽

Periodontal Ligament Cells ◽

Oral Rehydration ◽

Storage Media ◽

Rehydration Solution

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NATURAL MEDIA VS ARTIFICIAL MEDIA FOR PRESERVATION OF AVULSED TOOTH.-Original Reaserch

Clinical Dentistry ◽

10.33882/clinicaldent.13.25280 ◽

2019 ◽

Author(s):

MANOJ KALLURI ◽

TEJASWINI GUDURU

Keyword(s):

Periodontal Ligament ◽

Root Resorption ◽

Culture Media ◽

Tap Water ◽

Root Surface ◽

Pomegranate Juice ◽

Periodontal Ligament Cells ◽

Pulp Tissue ◽

Storage Media ◽

Contact Lens Solution

Dental avulsion may be defined as the displacement of the tooth from its socket as a result of trauma. The best possible treatment for an avulsed tooth is replanting it immediately (i.e., less than five minutes). Unfortunately, and most of the times avulsed teeth experience delayed replantation. The success of delayed replantation depends on the vitality of the periodontal ligament cells remaining on the root surface. The chances of root surface damage are higher when tooth replantation is not performed immediately or if the avulsed tooth is not stored in an adequate storage medium. This invariably leads to necrosis of pulp tissue, periodontal ligament cells and thus increasing the possibility of root resorption, which is the main cause of loss of replanted teeth. Isotonic medium is superior to dry storage in the storage of avulsed tooth. Natural storage media are tap water, milk, saline, saliva, coconut water, egg white, propolis extract, green tea extract, aloe vera, pomegranate juice, red mulberry etc. Synthetic storage media are Gatorade, contact lens solution, HBSS, viaspan, emdogain, culture media etc. In this article, we are going to discuss the most suitable media for interim storage of the avulsed tooth by comparing the natural and synthetic storage media. KeyWords: preservative, media, avulsed teeth, Dental tissue

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Effect of Milk Renewal on Cell Viability In Vitro at Different Time Frames

Brazilian Dental Journal ◽

10.1590/0103-6440201601164 ◽

2017 ◽

Vol 28 (4) ◽

pp. 435-439 ◽

Cited By ~ 2

Author(s):

Beatriz Dulcineia Mendes de Souza ◽

Ana Maria Hecke Alves ◽

Dayane Machado Ribeiro ◽

Luciane Geanini Pena dos Santos ◽

Claudia Maria de Oliveira Simões ◽

...

Keyword(s):

Cell Viability ◽

Tap Water ◽

Positive Control ◽

Negative Control ◽

Tetrazolium Salt ◽

Periodontal Ligament Fibroblasts ◽

Skimmed Milk ◽

Time Frames

Abstract The purpose of this study was to evaluate if the renewal of milk as a storage medium, every 12, 24 and 48 h, is able to increase its ability to maintain human periodontal ligament fibroblasts (PDLF) viability over time. PDLF were soaked in Minimum Essential Medium at 37 °C (MEM-37) (positive control), tap water (Water) (negative control) and in skimmed milk (44 wells) at 5 °C and 20 °C. The skimmed milk was renewed every 12 h (Milk-12), 24 h (Milk-24) and 48 h (Milk-48) in 11 wells of each plate, and the milk in the remaining 11 wells of each plate was maintained in situ (not renewed milk) (NRM). After 24, 48, 72, 96 and 120 h, cell viability was determined by the tetrazolium salt-based colorimetric (MTT) assay. Data were statistically analyzed by Kruskal-Wallis, Scheffé and Mann-Whitney tests (a=5%). At 5 °C, only Milk-48 was significantly better than NRM. At 20 °C, NRM was more effective than Milk-12 and Milk-24 in all time periods. In relation to the temperature (5 °C or 20 °C), renewal of milk at 5 °C was better in maintaining cell viability than the renewal at 20 °C. In conclusion, the renewal of milk was able to increase its ability to maintain cell viability only when performed every 48 h in milk maintained at 5 °C.

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EFFECT OF DİFFERENT AVULSED TOOTH STORAGE MEDİA ON İN VİTRO CELL VİABİLİTY OF HUMAN PERİODONTAL LİGAMENT FİBROBLASTS

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi ◽

10.17567/ataunidfd.915271 ◽

2021 ◽

pp. 362-366

Author(s):

Ahu DİKİLİTAŞ ◽

Fatih KARAASLAN ◽

Mehmet TAŞPINAR ◽

Filiz TAŞPINAR

Keyword(s):

Cell Viability ◽

Periodontal Ligament ◽

Periodontal Ligament Fibroblasts ◽

Storage Media ◽

Human Periodontal Ligament Fibroblasts

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Dragon's Blood Sap (Croton Lechleri) As Storage Medium For Avulsed Teeth: In Vitro Study Of Cell Viability

Brazilian Dental Journal ◽

10.1590/0103-6440201600987 ◽

2016 ◽

Vol 27 (6) ◽

pp. 751-756 ◽

Cited By ~ 5

Author(s):

Christine Men Martins ◽

Elizane Ferreira Hamanaka ◽

Thayse Yumi Hoshida ◽

Ana Maria Sell ◽

Mirian Marubayashi Hidalgo ◽

...

Keyword(s):

Cell Viability ◽

Periodontal Ligament ◽

Trypan Blue ◽

In Vitro Study ◽

Vitro Study ◽

Periodontal Ligament Cells ◽

Storage Medium ◽

Dragon’S Blood ◽

Storage Media

Abstract Tooth replantation success depends on the condition of cementum periodontal ligament after tooth avulsion; which is influenced by storage medium. The dragon's blood (Croton lechleri) sap has been suggested as a promising medium because it supports collagen formation and exhibits healing, anti-inflammatory and antimicrobial properties. Thus, the aim of this study was to evaluate the efficacy of dragon's blood sap as a storage medium for avulsed teeth through evaluation of functional and metabolic cell viability. This in vitro study compared the efficacy of different storage media to maintain the viability of human peripheral blood mononuclear and periodontal ligament cells. A 10% dragon's blood sap was tested while PBS was selected as its control. Ultra pasteurized whole milk was used for comparison as a commonly used storage medium. DMEM and distilled water were the positive and negative controls, respectively. The viability was assessed through trypan blue exclusion test and colorimetric MTT assay after 1, 3, 6, 10 and 24 h of incubation. The dragon's blood sap showed promising results due to its considerable maintenance of cell viability. For trypan blue test, the dragon's blood sap was similar to milk (p<0.05) and both presented the highest viability values. For MTT, the dragon's blood sap showed better results than all storage media, even better than milk (p<0.05). It was concluded that the dragon's blood sap was as effective as milk, the gold standard for storage medium. The experimental sap preserved the membrane of all cells and the functional viability of periodontal ligament cells.

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