Influence of Storage Media Containing Salvia officinalis on Survival of Periodontal Ligament Cells

2008 ◽  
Vol 9 (6) ◽  
pp. 17-24 ◽  
Author(s):  
Fatih Ozan ◽  
Zübeyde Akin Polat ◽  
Bektaş Tepe ◽  
Kürşat Er
Keyword(s):  
Cell Viability ◽  
Periodontal Ligament ◽  
Tap Water ◽  
Salvia Officinalis ◽  
The Other ◽  
Periodontal Ligament Cells ◽  
Storage Medium ◽  
Trypan Blue Exclusion ◽  
Storage Media ◽  
Pdl Cells

Aim The purpose of this study was to determine the ability of Salvia officinalis (S. officinalis) extracts to serve as a storage medium for the maintenance of periodontal ligament (PDL) cell viability of avulsed teeth. Methods and Materials PDL cells were obtained from healthy third molars and cultured in Dulbecco's Modified Eagle's Medium (DMEM). Cultures were subjected to 4, 2.5, 1.5, and 0.5% S. officinalis solutions, Hank's balanced salt solution (HBSS), phosphate buffered saline (PBS), and tap water. Tissue culture plates were incubated with experimental media at 37°C for 1, 3, 6, 12 or 24 hours. PDL cell viability was assessed by trypan blue exclusion. Statistical analysis of the data was performed by one-way analysis of variance (ANOVA) complemented by the Tukey's test. The level of significance was 5% (p< 0.05). Results The results showed 2.5% S. officinalis was a more effective storage medium than the other experimental solutions (p<0.05). Only at 1 hour and 3 hours was there found similar effect between 2.5% S. officinalis and HBSS. At 24 hours, 2.5% S. officinalis was found to be significantly better than the other solutions tested. Conclusion S. officinalis can be recommended as a suitable transport medium for avulsed teeth. Clinical Significance The findings of this study support the use of S. officinalis as another option for clinicians to use to store and transport avulsed teeth until reimplantation procedures can be done. Citation Özan F, Polat ZA, Tepe B, Er K. Influence of Storage Media Containing Salvia officinalis on Survival of Periodontal Ligament Cells. J Contemp Dent Pract 2008 September; (9)6:017-024.

scholarly journals Fibroblast Viability after Storage at 20 °C in Milk, Hank's Balanced Salt Solution and Coconut Water

2016 ◽  
Vol 27 (4) ◽  
pp. 404-407 ◽  
Author(s):  
Beatriz Dulcineia Mendes de Souza ◽  
Ana Maria Hecke Alves ◽  
Luciane Geanini Pena dos Santos ◽  
Claudia Maria de Oliveira Simões ◽  
Wilson Tadeu Felippe ◽  
...  
Keyword(s):  
Cell Viability ◽  
Salt Solution ◽  
Periodontal Ligament ◽  
Tap Water ◽  
Control Cell ◽  
Negative Control ◽  
Coconut Water ◽  
Periodontal Ligament Cells ◽  
Periodontal Ligament Fibroblasts ◽  
Storage Media

Abstract The objective of this study was to evaluate the effectiveness of various storage media at 20 °C in maintaining the viability of human periodontal ligament fibroblasts (HPLF) over time. HPLF were maintained at 20 °C in skim milk (SM), whole milk (WM), freshly prepared Hank's balanced salt solution (HBSS), Save-A-Tooth(r), natural coconut water (NCW), coconut water industrialized (ICW) and tap water (negative control) for 3, 6, 24, 48, 72, 96 and 120 h. Cells maintained in Minimal Essential Medium (MEM-37) at 37 °C served as a positive control. Cell viability was determined by MTT assay. Statistical analysis was performed by Kruskal-Wallis test and Scheffe test (α = 5%). From 24 h, NCW was significantly better in maintaining cell viability than all other tested storage media (p<0.05). SM and WM were significantly better than HBSS for up to 72 h. Save-A-Tooth(r) and ICW were the worst conservation storage media. In conclusion, the effectiveness of the tested storage media to maintain the viability of the periodontal ligament cells was as follows, in a descending order: NCW > MEM-37> SM and IM> HBSS> ICW > Save-A-Tooth(r)> tap water.

scholarly journals Dragon's Blood Sap (Croton Lechleri) As Storage Medium For Avulsed Teeth: In Vitro Study Of Cell Viability

2016 ◽  
Vol 27 (6) ◽  
pp. 751-756 ◽  
Author(s):  
Christine Men Martins ◽  
Elizane Ferreira Hamanaka ◽  
Thayse Yumi Hoshida ◽  
Ana Maria Sell ◽  
Mirian Marubayashi Hidalgo ◽  
...  
Keyword(s):  
Cell Viability ◽  
Periodontal Ligament ◽  
Trypan Blue ◽  
In Vitro Study ◽  
Vitro Study ◽  
Periodontal Ligament Cells ◽  
Storage Medium ◽  
Dragon’S Blood ◽  
Storage Media

Abstract Tooth replantation success depends on the condition of cementum periodontal ligament after tooth avulsion; which is influenced by storage medium. The dragon's blood (Croton lechleri) sap has been suggested as a promising medium because it supports collagen formation and exhibits healing, anti-inflammatory and antimicrobial properties. Thus, the aim of this study was to evaluate the efficacy of dragon's blood sap as a storage medium for avulsed teeth through evaluation of functional and metabolic cell viability. This in vitro study compared the efficacy of different storage media to maintain the viability of human peripheral blood mononuclear and periodontal ligament cells. A 10% dragon's blood sap was tested while PBS was selected as its control. Ultra pasteurized whole milk was used for comparison as a commonly used storage medium. DMEM and distilled water were the positive and negative controls, respectively. The viability was assessed through trypan blue exclusion test and colorimetric MTT assay after 1, 3, 6, 10 and 24 h of incubation. The dragon's blood sap showed promising results due to its considerable maintenance of cell viability. For trypan blue test, the dragon's blood sap was similar to milk (p<0.05) and both presented the highest viability values. For MTT, the dragon's blood sap showed better results than all storage media, even better than milk (p<0.05). It was concluded that the dragon's blood sap was as effective as milk, the gold standard for storage medium. The experimental sap preserved the membrane of all cells and the functional viability of periodontal ligament cells.

Viability and Reproducibility of Periodontal Ligament Cells on Avulsed Teeth Stored in Ham's F-10 Solution

2018 ◽  
Vol 42 (3) ◽  
pp. 203-207 ◽  
Author(s):  
Maryam Talebi ◽  
Iman Parisay ◽  
Jalil Tavakol afshari ◽  
Arezoo Shajiei ◽  
Mostafa Sofiani Ghadim
Keyword(s):  
Cell Viability ◽  
Mtt Assay ◽  
Periodontal Ligament ◽  
Tap Water ◽  
Skim Milk ◽  
Periodontal Ligament Cells ◽  
Pdl Cells ◽  
Experimental Media ◽  
Significant Difference ◽  
Better Than

Objectives: The purpose of the present study was to evaluate the efficacy of Ham's F-10 in maintaining the viability and reproducibility of PDL cells on avulsed teeth. Study design: Sixty mature, healthy extracted premolars were used. The experimental media used were Ham's F-10, Hank's balanced salt solution (HBSS), skim milk, and tap water (n = 15 specimens each). Cell viability was tested after 1, 3, 6, and 24 h storage in medium. Cell reproducibility was assessed by methyl-thiazol-tetrazolium (MTT) assay after1, 3, and 6 h storage in Ham's F-10, HBSS, and tap water. Results: The viability of PDL cells stored in Ham's F-10 and HBSS was significantly greater than that of samples stored in milk and tap water at all-time points (P&lt;0.001). A significant difference in cell viability between samples stored in Ham's F-10 and HBSS (favoring the former) was observed only at 6h (P=0.04). MTT assay results were significantly better for samples stored in Ham's F-10 and HBSS than for those stored in tap water (P&lt;0.001), with a significant difference between Ham's F-10 and HBSS observed only at 3h (P&lt;0.001). Conclusions: Ham's F-10 is capable of preserving PDL cells viable and reproducible better than milk and tap water and similar to HBSS.

scholarly journals In Vitro Periodontal Ligament Cell Viability in Different Storage Media

2016 ◽  
Vol 27 (4) ◽  
pp. 408-411 ◽  
Author(s):  
Meenakshi Sharma
Keyword(s):  
Cell Viability ◽  
Periodontal Ligament ◽  
Egg White ◽  
Periodontal Ligament Cells ◽  
Periodontal Ligament Cell ◽  
Trypan Blue Exclusion ◽  
Storage Media ◽  
Significant Difference ◽  
Rice Water

Abstract The aim of this study was to evaluate the viability of periodontal ligament cells of avulsed teeth in three different storage media. Forty-five mature premolars extracted for orthodontic therapeutic purposes were randomly and equally divided into three groups according to the storage medium: milk (control), rice water and egg white. After placing extracted teeth for 30 min in storage media, the scrapings of the periodontal ligament (PDL) were collected in Falcon tubes containing collagenase in 2.5 mL of phosphate buffer saline and were incubated for 30 min and centrifuged for 5 min at 800 rpm. Cell viability was analyzed by Trypan blue exclusion. Rice water had a significantly higher number of viable cells compared to egg white and milk. There was no statistically significant difference between egg white and milk. Rice water may be able to maintain PDL cell viability of avulsed teeth better than egg white or milk.

scholarly journals STORAGE MEDIUM FOR AVULSED TEETH: A LITERATURE REVIEW

2021 ◽  
Vol 9 (08) ◽  
pp. 874-886
Author(s):  
Navpreet Kaur ◽  
Nikhil Srivastava ◽  
Vivek Rana ◽  
Noopur Kaushik ◽  
Tushar Pruthi
Keyword(s):  
Literature Review ◽  
Periodontal Ligament ◽  
Storage Medium ◽  
Avulsion Injury ◽  
Storage Media ◽  
Pdl Cells ◽  
Traumatic Dental Injuries ◽  
Dental Injuries

Avulsion injury is one of the most severe types of traumatic dental injuries. Following avulsion, periodontal ligament tissues are injured and the vessels and nerves of the pulp rupture at the apical foramen which causes pulp necrosis. In studies it was reported that the key to retention of the knocked-out teeth was to maintain the viability of the periodontal ligament. Storage media plays an important role in preserving the viability of PDL cells during extra alveolar time. This article highlights the different storage medias available for avulsed teeth, along with their merits and demerits.

scholarly journals Comparative in vitro study of the effectiveness of Green tea extract and common storage media on periodontal ligament fibroblast viability

2016 ◽  
Vol 10 (03) ◽  
pp. 408-412 ◽  
Author(s):  
Fahimeh Adeli ◽  
Ebrahim Zabihi ◽  
Zeinab Abedian ◽  
Samane Gharekhani ◽  
Mahdi Pouramir ◽  
...  
Keyword(s):  
Green Tea ◽  
Periodontal Ligament ◽  
Sucrose Solution ◽  
Tap Water ◽  
Green Tea Extract ◽  
Periodontal Ligament Cells ◽  
Tetrazolium Salt ◽  
Storage Media ◽  
Good Ability ◽  
Tea Extract

ABSTRACT Objective: Green tea extract (GTE) was shown to be effective in preserving periodontal ligament fibroblasts (PDLFs) of avulsed teeth. This study aimed at determining the potential of GTE in preserving the viability of PDLFs comparing with different storage media. Materials and Methods: Periodontal ligament cells were obtained from freshly extracted healthy impacted third molars and cultured in Dulbecco's Modified Eagle Medium (DMEM). Cell viability was determined by storing the cells in seven media; DMEM, tap water, Hank's balanced salt solution (HBSS), whole milk, hypotonic sucrose solution, GTE, and GTE + sucrose for 1, 2, 4, and 24 h at 37°C using tetrazolium salt-based colorimetric (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) assay. Statistical analysis was performed by one-way analysis of variance and post hoc tests. Results: GTE showed significantly higher protective effect than HBSS at 2, 4, and 24 h (P = 0.009, P = 0.02, P = 0.016), DMED at 2 h (P = 0.003), and milk at 4 h (P = 0.039). Conclusion: Although with undesirable osmolality and pH, GTE had a good ability in preserving the PDLFs comparing with other studied media.

Impact of nicotine on the interplay between human periodontal ligament cells and CD4+ T cells

2016 ◽  
Vol 35 (9) ◽  
pp. 983-990 ◽  
Author(s):  
Xin Ge ◽  
Ying-Feng Liu ◽  
Yong Wong ◽  
Li-Zheng Wu ◽  
Ling Tan ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cell Viability ◽  
Periodontal Ligament ◽  
Tobacco Consumption ◽  
Periodontal Ligament Cells ◽  
Nicotine Treatment ◽  
Pdl Cells ◽  
Induced Apoptosis ◽  
The Impact

Periodontitis is a common infectious disease associated with destruction of periodontal ligaments and alveolar bones. CD4+ T cell-mediated immune response is involved in the progression of periodontitis. Tobacco consumption increases the risk of periodontal disease. However, the impact of nicotine on the interaction between human periodontal ligament (PDL) cells and CD4+ T cells remains unrevealed. Our study aims to investigate the effect of nicotine on PDL cells and the cocultured CD4+ T cells. The PDL cell cultures were established by explants from healthy individuals, exposed to nicotine or α-bungarotoxin (α-BTX), and incubated solely or in combination with CD4+ T cells. Afterwards, cell viability, secreted cytokines, and matrix metalloproteinases (MMPs) were evaluated. In monoculture of PDL cells, nicotine dramatically repressed cell viability and increased apoptosis. Meanwhile, α-BTX largely reversed the nicotine-induced apoptosis and increased viability of PDL cells. Compared with the monoculture, MMP-1, MMP-3, interleukin (IL)-1β, IL-6, IL-17, and IL-21 in supernatant of cocultures were markedly elevated after treatment with nicotine. Moreover, α-BTX significantly attenuated nicotine-triggered production of these components either in mono- or co-cultures. In addition, PDL cell-derived CXCL12 following nicotine treatment recruited CD4+ T cells. Above all, nicotine deteriorated periodontitis partially by promoting PDL cell–CD4+ T cell-mediated inflammatory response and matrix degradation.

scholarly journals NATURAL MEDIA VS ARTIFICIAL MEDIA FOR PRESERVATION OF AVULSED TOOTH.-Original Reaserch

2019 ◽  
Author(s):  
MANOJ KALLURI ◽  
TEJASWINI GUDURU
Keyword(s):  
Periodontal Ligament ◽  
Root Resorption ◽  
Culture Media ◽  
Tap Water ◽  
Root Surface ◽  
Pomegranate Juice ◽  
Periodontal Ligament Cells ◽  
Pulp Tissue ◽  
Storage Media ◽  
Contact Lens Solution

Dental avulsion may be defined as the displacement of the tooth from its socket as a result of trauma. The best possible treatment for an avulsed tooth is replanting it immediately (i.e., less than five minutes). Unfortunately, and most of the times avulsed teeth experience delayed replantation. The success of delayed replantation depends on the vitality of the periodontal ligament cells remaining on the root surface. The chances of root surface damage are higher when tooth replantation is not performed immediately or if the avulsed tooth is not stored in an adequate storage medium. This invariably leads to necrosis of pulp tissue, periodontal ligament cells and thus increasing the possibility of root resorption, which is the main cause of loss of replanted teeth. Isotonic medium is superior to dry storage in the storage of avulsed tooth. Natural storage media are tap water, milk, saline, saliva, coconut water, egg white, propolis extract, green tea extract, aloe vera, pomegranate juice, red mulberry etc. Synthetic storage media are Gatorade, contact lens solution, HBSS, viaspan, emdogain, culture media etc. In this article, we are going to discuss the most suitable media for interim storage of the avulsed tooth by comparing the natural and synthetic storage media. KeyWords: preservative, media, avulsed teeth, Dental tissue

Evaluation of Aloevera Gel as a Storage Medium in Maintaining the Viability of Periodontal Ligament Cells - An in Vitro Study

2016 ◽  
Vol 40 (1) ◽  
pp. 49-52 ◽  
Author(s):  
Punit Fulzele ◽  
Sudhindra Baliga ◽  
Nilima Thosar ◽  
Debaprya Pradhan
Keyword(s):  
Drinking Water ◽  
Periodontal Ligament ◽  
In Vitro Study ◽  
Periodontal Ligament Cells ◽  
Blue Dye ◽  
Storage Medium ◽  
Viable Cells ◽  
Pdl Cells ◽  
Significant Difference

Objective: To investigate the effectiveness of aloevera gel as a new storage medium in maintaining the viability of periodontal ligament cells. Study design: Premolars extracted for orthodontic reason were obtained. Confluent monolayers of fibroblasts were grown by cell culture method from the PDL cells isolated from the extracted teeth. One ml of this cell suspension was transferred to wells of culture plates, incubated for 24 hrs, followed by exposure to the three experimental media, Hank's balanced salt solution (HBSS), aloevera gel, and packaged drinking water. These plates were then assessed for viable cells using trypan blue dye exclusion test with haemocytometer after 15, 30, 60, 90 and 120 mins. The results obtained were statistically analysed using one-way analysis of variance (ANOVA). Results: At 15 min, HBSS presented maximum mean percentage of viable PDL cells (89%), followed by aloevera at 81% and packaged drinking water at 10%. Aloevera demonstrated 71%, 59%, 57% viable cells at 30, 60, 90 mins respectively. At 120 min, HBSS presented 57% viable cells followed by aloevera gel (45%) and packaged drinking water (3%). No statistical significant difference was observed between HBSS and aloevera gel. Conclusions: Within the parameters of this study, both aloevera gel and HBSS were effective in maintaining the viability of PDL cells. Hence, aloevera gel could be used as a storage media for avulsed tooth in situations where availability of HBSS is in question.

A New Storage Medium for an Avulsed Tooth

2008 ◽  
Vol 9 (6) ◽  
pp. 25-32 ◽  
Author(s):  
Abbas Ali Khademi ◽  
Saeed Saei ◽  
Mohammad Reza Mohajeri ◽  
Nooshin Mirkheshti ◽  
Fatima Ghassami ◽  
...  
Keyword(s):  
Cell Viability ◽  
Tap Water ◽  
Egg White ◽  
Positive Control ◽  
Negative Control ◽  
Subsequent Treatment ◽  
Blue Staining ◽  
Storage Medium ◽  
Pdl Cells ◽  
Significant Difference

Aim The purpose of this study is to determine the efficacy of egg white in maintaining the viability of human periodontal ligament (PDL) cells on avulsed teeth. Methods and Materials The experimental media were: egg white, milk, Hanks’ Balanced Salt Solution (HBSS) as the positive control, and tap water as the negative control. The storage times were 1, 2, 4, 8, and 12 hours. Extracted premolar teeth of healthy individuals were rinsed in the media. After trypsinization and subsequent treatment in collagenase, cell viability was determined using trypan blue staining. The two-way analysis of variance (ANOVA) statistical test was used to compare the results among different media. Results There was no difference in the cell viability between egg white and HBSS media, but there was a statistically significant difference between the viability of PDL cells in egg white medium in comparison with milk (P<0.05) and water (P<0.05). Conclusion Egg white could be suggested as a suitable storage medium. Its principle advantage is its availability. Citation Khademi AA, Saei S, Mohajeri MR, Mirkheshti N, Ghassami F, Torabi nia N, Alavi SA. A New Storage Medium for an Avulsed Tooth. J Contemp Dent Pract 2008 September; (9)6:025-032.

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