Effects of Long-Term Storage on Plasmid Stability in Bacillus anthracis

Abstract This study investigated weight changes of seven different light-cured composite restorative materials, one polyacid glass ionomer compomer, and one light-cured glass-ionomer cement following short-term and long-term storage in water. Two packable composites, three universal (hybrid) composites, one microglass composite, one polyacid glass ionomer resin composite (compomer), one microhybrid low-viscosity (flowable) composite, and one light cured glass ionomer composite cement were evaluated in this study. The weight changes of these specimens were measured daily (short-term storage), and they were measured after six weeks (long-term storage) using an electronic analytical balance. A significant difference was found in Ionoliner, Dyract AP, Opticor flow, Charisma, and Solitare 2, but no significant difference was found in the others (Filtek Z 250, Filtek P60, TPH Spectrum, and Valux Plus). Weight change showed a tendency to increase with the time of water storage. The greatest weight change occurred in light-cured glass ionomer composite cement (Ionoliner), which is followed in order by the weight changes in Dyract AP, Opticor Flow, Charisma, Solitare 2, Filtek Z250, Filtek P60, TPH Spectrum; Valux Plus had the least amount of change. Citation Keyf F, Yalcin F. The Weight Change of Various Light-Cured Restorative Materials Stored in Water. J Contemp Dent Pract 2005 May;(6)2:072-079.

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Viability and Engraftment of Hematopoietic Progenitor Cells (HPC) After Long Term Cryopreservation: Effect of Diagnosis and % Dimethylsulfoxide (DMSO) Concentration.

Blood ◽

10.1182/blood.v114.22.3161.3161 ◽

2009 ◽

Vol 114 (22) ◽

pp. 3161-3161

Author(s):

Muthu Kumaran Veeraputhiran ◽

John Theus ◽

Gina Pesek ◽

Bart Barlogie ◽

Michele H. Cottler-Fox

Keyword(s):

Storage Time ◽

Conflicts Of Interest ◽

Population Data ◽

T Test ◽

Hematopoietic Progenitor ◽

Long Term Storage ◽

Significant Difference ◽

Dmso Concentration ◽

Term Storage

Abstract Abstract 3161 Poster Board III-98 Introduction There are few reports of HPC viability after more than 9 y of storage. It has been suggested that HPC from myeloma patients (pts) may survive storage less well than those from other diseases. The appropriate concentration of DMSO for long term storage is unclear. Our program has collected and stored HPC since November 1989, changing from 5% DMSO to 10% DMSO in August 2000. We present a retrospective analysis of viability by diagnosis and DMSO concentration in pts who underwent autologous transplant using HPC after long-term storage (up to 17.8 y). Materials and Methods HPC were harvested and preserved using a controlled rate freezer and 5% or 10% DMSO, then stored in liquid nitrogen. Viability was tested by flow cytometry using unwashed samples and looking at propidium iodide or 7-AAD uptake within the CD34+ population. Data from 242 samples were analyzed (224 myeloma pts and 18 other diagnoses): 100 consecutively thawed samples with storage time <1 y (all 10% DMSO), 100 consecutive samples stored from 5-9 y ( 7 samples, 10% and 93 samples, 5% DMSO), and all samples stored and used for transplant after >9y (42 samples, 5% DMSO). No statistically significant difference was seen in viability between <1y and 5-9y, so these groups were combined and compared to >9y. White blood cell (WBC) and platelet engraftment were compared for the 2 groups using an unpaired t test. Results Mean viability for the 0-9y and >9 y groups were 91.62% and 92.79% respectively, with no statistically significant difference in viability between groups (p=0.28). Using a 2-tailed t test, no difference was seen in viability based on diagnosis (p=0.19) or between 5% and 10% DMSO (p= 0.12). No difference was seen in WBC engraftment (p=0.28) or platelet engraftment between groups (p=0.57). Discussion HPC collections remained viable after 17.8 years, the longest time reported for use after storage to date. No difference in viability was seen with 5% or 10% DMSO, or between myeloma and other diseases. Storage time did not affect engraftment. Disclosures No relevant conflicts of interest to declare.

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275 Effect of Fruit Quality, Ethylene Evolution, and Storage in Apple `Tsugaru' as Influenced by Aminoethoxyvinylglycine and Several Surfactants

HortScience ◽

10.21273/hortsci.35.3.438e ◽

2000 ◽

Vol 35 (3) ◽

pp. 438E-438

Author(s):

Chung-Kil Kang

Keyword(s):

Fruit Quality ◽

Soluble Solids ◽

Fresh Market ◽

Ethylene Evolution ◽

Long Term Storage ◽

Color Development ◽

Significant Difference ◽

And Storage ◽

Term Storage

This experiment was conducted to evaluate the effect of fruit quality, ethylene evolution, and storage in apple `Tsugaru' as influenced by aminoethoxyvinylglycine (AVG) and several surfactants. When treated with AVG + Silwet L-77, there was little significant difference in soluble solids and acidity as compared with control, but dichlorprop treatment was significantly increased in soluble solids and decreased in acidity. Color development was decreased when treated with AVG + Silwet L-77. AVG + Silwet L-77 treatment decreased ethylene evolution, and increased storage. It can be concluded that fruit can be left on trees longer and still maintain storability, and more fruit is able to go to fresh market from long-term storage, which increases the marketability of apple.

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CRYOPRESERVATION OF TRUE-SEED AND EMBRYO OF MAIZE AND SOYBEAN FOR LONG-TERM STORAGE

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v2n2.2001.31-36 ◽

2016 ◽

Vol 2 (2) ◽

pp. 31

Author(s):

Enny Sudarmonowati ◽

I. Fitryatmi ◽

S. Sadjad

Keyword(s):

Liquid Nitrogen ◽

Rapid Freezing ◽

Soybean Seeds ◽

Long Term Storage ◽

Significant Difference ◽

Long Term Preservation ◽

Term Storage ◽

Cryoprotectant Solution ◽

Excised Embryos

Study on cryopreservation of Indonesian local cultivars and improved varieties of maize and soybean has never been done. This method may be used for long-term preservation of seeds of maize and soybean. In this study, the method was applied to maize and soybean, Arjuna and Wilis respectively, as a model for preserving germplasm of ortodox seeds. Whole seeds and excised embryos of both varieties were subjected to two methods of cryopreservation, i.e., two-stage cooling and rapid freezing with or without 15% dimethyl sulfoxide (DMSO) as cryoprotectant solution prior to immersion in liquid nitrogen (-196oC). Results indicated that there was no significant difference between the use of DMSO for both species in terms of viability, although pretreatment in DMSO was slightly reduced the percentage of viability of both species. Slow freezing to -30oC prior to immersion in the liquid nitrogen could give as high as 76.67% and 51.67% surviving whole seeds of maize and soybean, respectively. Preserving excised embryos of maize in the liquid nitrogen using either slow or rapid freezing significantly reduced the percentage of viability from 20-76.67% to 5-18.33% (four folds) depending on treatments applied. Results also showed that one day or 15 minutes of immersion of samples in the liquid nitrogen gave rise to similar values of viability of maize and soybean, i.e., 20-60% and 20-51.67%, respectively depending on treatments applied. These results implied that for long-term storage of maize and soybean seeds as they could survive at the rate of 76.67% and 51.67% respectively, the seed can be treated by prefreezing to -30oC without the presence of DMSO prior to immersion in liquid nitrogen.

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The effect of long-term storage on the microleakage of composite resin restorations: qualitative and quantitative evaluation

Pesquisa Odontológica Brasileira ◽

10.1590/s1517-74912003000300011 ◽

2003 ◽

Vol 17 (3) ◽

pp. 261-266

Author(s):

Fernanda Tranchesi Sadek ◽

Sandra Kiss Moura ◽

Rafael Yagüe Ballester ◽

Antonio Muench ◽

Paulo Eduardo Capel Cardoso

Keyword(s):

Composite Resin ◽

Class Ii ◽

Adhesive Systems ◽

Qualitative And Quantitative ◽

Long Term Storage ◽

Significant Difference ◽

Blue Solution ◽

Composite Resin Restorations ◽

Term Storage

The aim of this study was to evaluate the influence of storage periods of 24 hours and 3 months on the microleakage of class II cavities. Two methods of assessing microleakage were also compared. Class II cavities were prepared in sound human molars. MO cavities were restored using ABF experimental (Kuraray Medical Inc.) + Z250 composite resin (3M ESPE), and DO cavities were restored using Single Bond (3M ESPE) + Z250. After different storage periods, specimens were thermocycled, immersed in a dye (0.5% methylene blue solution for 4 h) and longitudinally sectioned. Dye penetration was scored according to a 0-4 scale. The extent of microleakage was measured using the ImageLab 2000 program. A statistically significant correlation was verified between both evaluation methods (r = 0.978, p < 0.001). ANOVA revealed a statistically significant difference between the tested adhesive systems regarding microleakage (p < 0.001), although it was not influenced by the different storage periods.

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Evaluation of the effects of long-term storage of bovine ear notch samples on the ability of 2 diagnostic assays to identify calves persistently infected with bovine viral diarrhoea virus

Journal of the South African Veterinary Association ◽

10.4102/jsava.v82i1.29 ◽

2011 ◽

Vol 82 (1) ◽

Author(s):

F. Khan ◽

J. H. Vorster ◽

M. Van Vuuren ◽

P. Mapham

Keyword(s):

Bovine Viral Diarrhoea Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Bovine Viral Diarrhoea ◽

Persistently Infected ◽

Diagnostic Assays ◽

Long Term Storage ◽

Diarrhoea Virus ◽

Significant Difference ◽

Term Storage

Research aimed at optimising diagnostic laboratory procedures is central to the development of effective bovine viral diarrhoea virus (BVDV) control programmes. BVDV is a singlestranded RNA virus that crosses the placenta to infect foetuses, resulting in reproductive losses due to foetal death or persistently infected calves that die early in life. Persistently infected animals are widely accepted to be the primary reservoir of BVDV and the largest source of infection. This poses important challenges to overall animal/herd health and can cause major losses to the cattle industry. Long-term storage of bovine ear notch samples from calves persistently infected with BVDV may adversely affect the ability of diagnostic assays to detect the virus efficiently. In order to test this hypothesis, ear notch samples from 7 animals were divided into 2 groups. One set was subjected to prompt formalin fixation and the other set stored either as fresh samples without preservatives at –2 °C, or soaked overnight in phosphate buffered saline followed by freezing of the supernatant fluid at –2 °C. Frozen ear notches and ear notch supernatant yielded positive results with an antigen- capture, enzyme linked immunosorbent assay (AC-ELISA) for the duration of the study (6 months) and optical density (OD) values remained significantly within range. There was no significant difference between storing fresh ear notch samples or PBS at –2 °C. However, positive immunohistochemistry (IHC) staining on formalin fixed ear notches started to fade between Day 17 and Day 29 when stored at room temperature. It was concluded that fresh ear notches could safely be stored at –2 °C for a period of 6 months prior to testing for BVD viral antigens.

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МОРФОЛОГІЧНИЙ АНАЛІЗ СПЕРМИ БУГАЇВ ДОВГОТРИВАЛОГО ЗБЕРІГАННЯ

Вісник Полтавської державної аграрної академії ◽

10.31210/visnyk2014.01.31 ◽

2018 ◽

pp. 130-133

Author(s):

А. О. Ляшенко

Keyword(s):

Genetic Resources ◽

Liquid Nitrogen ◽

Morphological Characteristics ◽

Bull Semen ◽

Long Term Storage ◽

Significant Difference ◽

Bull Sperm ◽

Dairy Breed ◽

Term Storage

Проведено дослідження морфологічних характе-ристик деконсервованих сперматозоїдів бугаїв різнихпорід Банку генетичних ресурсів за умов довготрива-лого зберігання в рідкому азоті. Встановлено вірогід-ну різницю між показниками патологічних і мертвихформ сперміїв бугаїв за різних термінів зберігання.У результаті проведених досліджень (за термінівзберігання 10–45 років) у бугаїв молочних порід спо-стерігались вищі за норму значення патологічнихформ сперматозоїдів у середньому на 2,5 %, окрімсперми української чорно-рябої молочної породи, а успермі бугаїв симентальської та м’ясних порід вищі усередньому на 5 %. The morphological characteristics of frozen bull sperm of different breeds the Bank of genetic resources the long-term storage in liquid nitrogen were studied. A significant difference between the indicators of pathological and dead forms of bull semen with different terms of storage was set. The result studies, by the terms of storage 10-45 years, in bulls of dairy breeds were observed above normal of pathological forms of sperm in an average of 2,5 %, except for sperm Ukrainian Black Pied dairy breed. In semen bulls of Simmental and beef breeds above an average of 5 %.

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CRYOPRESERVATION OF TRUE-SEED AND EMBRYO OF MAIZE AND SOYBEAN FOR LONG-TERM STORAGE

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v2n2.2001.p31-36 ◽

2016 ◽

Vol 2 (2) ◽

pp. 31

Author(s):

Enny Sudarmonowati ◽

I. Fitryatmi ◽

S. Sadjad

Keyword(s):

Liquid Nitrogen ◽

Rapid Freezing ◽

Soybean Seeds ◽

Long Term Storage ◽

Significant Difference ◽

Long Term Preservation ◽

Term Storage ◽

Cryoprotectant Solution ◽

Excised Embryos

Study on cryopreservation of Indonesian local cultivars and improved varieties of maize and soybean has never been done. This method may be used for long-term preservation of seeds of maize and soybean. In this study, the method was applied to maize and soybean, Arjuna and Wilis respectively, as a model for preserving germplasm of ortodox seeds. Whole seeds and excised embryos of both varieties were subjected to two methods of cryopreservation, i.e., two-stage cooling and rapid freezing with or without 15% dimethyl sulfoxide (DMSO) as cryoprotectant solution prior to immersion in liquid nitrogen (-196oC). Results indicated that there was no significant difference between the use of DMSO for both species in terms of viability, although pretreatment in DMSO was slightly reduced the percentage of viability of both species. Slow freezing to -30oC prior to immersion in the liquid nitrogen could give as high as 76.67% and 51.67% surviving whole seeds of maize and soybean, respectively. Preserving excised embryos of maize in the liquid nitrogen using either slow or rapid freezing significantly reduced the percentage of viability from 20-76.67% to 5-18.33% (four folds) depending on treatments applied. Results also showed that one day or 15 minutes of immersion of samples in the liquid nitrogen gave rise to similar values of viability of maize and soybean, i.e., 20-60% and 20-51.67%, respectively depending on treatments applied. These results implied that for long-term storage of maize and soybean seeds as they could survive at the rate of 76.67% and 51.67% respectively, the seed can be treated by prefreezing to -30oC without the presence of DMSO prior to immersion in liquid nitrogen.

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Modelling of Moisture Movement in Wood during Outdoor Storage

Nonlinear Analysis Modelling and Control ◽

10.15388/na.2001.6.1.15210 ◽

2001 ◽

Vol 6 (2) ◽

pp. 3-14 ◽

Cited By ~ 23

Author(s):

R. Baronas ◽

F. Ivanauskas ◽

I. Juodeikienė ◽

A. Kajalavičius

Keyword(s):

Experimental Data ◽

Finite Difference ◽

Climatic Conditions ◽

Two Dimensional ◽

Moisture Movement ◽

Finite Difference Technique ◽

Long Term Storage ◽

Space Formulation ◽

Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

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