Susceptibility of Biomphalaria glabrata submitted to concomitant infection with Angiostrongylus costaricensis and Schistosoma mansoni

Abstract The easy adaptation of Angiostrongylus costaricensis, nematode responsible for abdominal angiostrongyliasis to several species of terrestrial and freshwater molluscs and the differences observed in the interactions of trematodes with their intermediate hosts have induced us to study the concomitant infection of Biomphalaria glabrata with Schistosoma mansoni and A. costaricensis. Prior exposure of B. glabrata to A. costaricensis (with an interval of 48 hours), favored the development of S. mansoni, observing higher infection rate, increased release of cercariae and increased survival of molluscs, when compared to molluscs exposed only to S. mansoni. Prior exposure of B. glabrata to A. costaricensis and then to S. mansoni also enabled the development of A. costaricensis since in the ninth week of infection, higher amount of A. costaricensis L3 larvae was recovered (12 larvae / mollusc) while for molluscs exposed only to A. costaricensis, the number of larvae recovered was lower (8 larvae / mollusc). However, pre-exposure of B. glabrata to S. mansoni (with an interval of 24 hours), and subsequently exposure to A. costaricensis proved to be very harmful to B. glabrata, causing extensive mortality of molluscs, reduced pre-patent period to release cercariae and greater recovery of L3 A. costaricensis larvae.

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Energy constraint exerted by the parasite Schistosoma mansoni on the locomotion of its snail host, Biomphalaria glabrata

Canadian Journal of Zoology ◽

10.1139/z96-068 ◽

1996 ◽

Vol 74 (4) ◽

pp. 594-598 ◽

Cited By ~ 6

Author(s):

Claudia Gérard

Keyword(s):

Resource Allocation ◽

Schistosoma Mansoni ◽

Biomphalaria Glabrata ◽

Snail Host ◽

Locomotory Activity ◽

Patent Period ◽

Energy Constraint

The consequences of the constraint caused by the parasite Schistosoma mansoni on the locomotory activity of its snail host, Biomphalaria glabrata, were studied during the patent period. Rates of locomotion were determined 6 times per 24-h period for juvenile and adult snails with single-miracidium infections, then compared with those of healthy snails of the same age. The locomotory activity of infected snails was the same during the day and at night, whereas control snails moved less at night than during the day. The locomotion of snails infected when immature was similar to that of the controls during the day and superior at night. The locomotion of snails infected when mature decreased regularly during patency and clearly decreased in comparison with that of healthy snails. The results are interpreted in terms of energy constraint on the mode of resource allocation of the host due to the parasite.

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The effects of temperature change on the infection rate of Biomphalaria glabrata with Schistosoma mansoni

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02762006000200016 ◽

2006 ◽

Vol 101 (2) ◽

pp. 223-224 ◽

Cited By ~ 12

Author(s):

João R Coelho ◽

Fernando SM Bezerra

Keyword(s):

Schistosoma Mansoni ◽

Temperature Change ◽

Infection Rate ◽

Biomphalaria Glabrata ◽

Effects Of Temperature

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Molluscicidal and parasiticidal activities of Eryngium triquetrum essential oil on Schistosoma mansoni and its intermediate snail host Biomphalaria glabrata, a double impact

Parasites & Vectors ◽

10.1186/s13071-020-04367-w ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Ronaldo de Carvalho Augusto ◽

Nadjiya Merad ◽

Anne Rognon ◽

Benjamin Gourbal ◽

Cédric Bertrand ◽

...

Keyword(s):

Essential Oil ◽

Schistosoma Mansoni ◽

Vector Control ◽

Biological Effects ◽

Biomphalaria Glabrata ◽

Snail Host ◽

Intermediate Hosts ◽

Egg Hatching ◽

Toxic Activity ◽

Oil Extract

Abstract Background Freshwater snails are the intermediate hosts of a large variety of trematode flukes such as Schistosoma mansoni responsible for one of the most important parasitic diseases caused by helminths, affecting 67 million people worldwide. Recently, the WHO Global Vector Control Response 2017–2030 (GVCR) programme reinforced its message for safer molluscicides as part of required strategies to strengthen vector control worldwide. Here, we present the essential oil from Eryngium triquetrum as a powerful product with molluscicide and parasiticide effect against S. mansoni and the snail intermediate host Biomphalaria glabrata. Methods In the present study, we describe using several experimental approaches, the chemical composition of E. triquetrum essential oil extract and its biological effects against the snail B. glabrata and its parasite S. mansoni. Vector and the free-swimming larval stages of the parasite were exposed to different oil concentrations to determine the lethal concentration required to produce a mortality of 50% (LC50) and 90% (LC90). In addition, toxic activity of this essential oil was analyzed against embryos of B. glabrata snails by monitoring egg hatching and snail development. Also, short-time exposure to sublethal molluscicide concentrations on S. mansoni miracidia was performed to test a potential effect on parasite infectivity on snails. Mortality of miracidia and cercariae of S. mansoni is complete for 5, 1 and 0.5 ppm of oil extract after 1 and 4 h exposure. Results The major chemical component found in E. triquetrum oil determined by GC-FID and GC/MS analyses is an aliphatic polyacetylene molecule, the falcarinol with 86.9–93.1% of the total composition. The LC50 and LC90 values for uninfected snails were 0.61 and 1.02 ppm respectively for 24 h exposure. At 0.5 ppm, the essential oil was two times more toxic to parasitized snails with a mortality rate of 88.8 ± 4.8%. Moderate embryonic lethal effects were observed at the concentration of 1 ppm. Severe surface damage in miracidia was observed with a general loss of cilia that probably cause their immobility. Miracidia exposed 30 min to low concentration of plant extract (0.1 ppm) were less infective with 3.3% of prevalence compare to untreated with a prevalence of 44%. Conclusions Essential oil extracted from E. triquetrum and falcarinol must be considered as a promising product for the development of new interventions for schistosomiasis control and could proceed to be tested on Phase II according to the WHO requirements.

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Behavior of Angiostrongylus costaricensis in planorbids

Brazilian Journal of Biology ◽

10.1590/s1519-69842006000200003 ◽

2006 ◽

Vol 66 (1b) ◽

pp. 199-204 ◽

Cited By ~ 5

Author(s):

N. M. S. Banevicius ◽

E. M. Zanotti-Magalhães ◽

L. A. Magalhães ◽

A. X. Linhares

Keyword(s):

Schistosoma Mansoni ◽

Biomphalaria Glabrata ◽

The Other ◽

Intermediate Hosts ◽

Nematode Larvae ◽

Third Stage ◽

Natural Hosts ◽

Two Populations ◽

Terrestrial Mollusks

Some terrestrial mollusks are natural hosts of Angiostrongylus costaricensis. In the laboratory, this nematode can be maintained in certain planorbids, which are aquatic mollusks and intermediate hosts of Schistosoma mansoni. Mollusks can be infected with Angiostrongylus costaricensis by ingestion of or active penetration by the first-stage larvae. In this work we assessed the ability of Biomphalaria glabrata to attract first-stage larvae of A. costaricensis. Movement of the nematode larvae towards the mollusks was observed after 15 min, 30 min and 1 h. B. glabrata did not attract the first-stage larvae of A. costaricensis in any of the three intervals. The susceptibility of two populations of Biomphalaria tenagophila to infection by A. costaricensis was also determined. One population was genetically selected for the susceptibility to S. mansoni while the other was not. Third-stage larvae were recovered from the snails 30 days after exposure of the two populations to 120 first-stage larvae. All the mollusks were infected. However, a significantly higher number of third-stage larvae were recovered in mollusks not genetically selected.

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Compatibility of Schistosoma mansoni Cameroon and Biomphalaria pfeifferi Senegal

Parasitology ◽

10.1017/s0031182099006708 ◽

2000 ◽

Vol 121 (5) ◽

pp. 501-505 ◽

Cited By ~ 19

Author(s):

V. R. SOUTHGATE ◽

L. A. TCHUEM TCHUENTÉ ◽

A. THÉRON ◽

J. JOURDANE ◽

A. LY ◽

...

Keyword(s):

Circadian Rhythm ◽

Schistosoma Mansoni ◽

Infection Rate ◽

Post Infection ◽

Vectorial Capacity ◽

Patent Period ◽

Snail Infection ◽

Biomphalaria Pfeifferi ◽

Emergence Pattern ◽

The Mean

The vectorial capacity of Biomphalaria pfeifferi from Ndiangue, Senegal, was investigated with an allopatric isolate of Schistosoma mansoni from Nkolbisson, Cameroon. The snail infection rate after exposure to a single miracidium per snail (MD1) was 56·3%, and 91·6% for snails exposed to 5 miracidia per snail (MD5). The minimum pre-patent period was 21 days. The mean total cercarial production for the MD1 group was 18511 cercariae per snail, and 9757 cercariae for the MD5 group. The maximum production of cercariae for 1 day was 4892 observed in a snail from the MD1 group at day 43 post-infection. The mean longevity of snails was higher in group MD1 (88 days p.i.) than in group MD5 (65 days p.i.). The chronobiological emergence pattern revealed a circadian rhythm with one shedding peak at mid-day. Comparisons are made with the vectorial capacity of the sympatric combination of B. pfeifferi Senegal/S. mansoni Senegal.

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A potential vector of Schistosoma mansoni in Uruguay

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02761989000300001 ◽

1989 ◽

Vol 84 (3) ◽

pp. 281-288 ◽

Cited By ~ 37

Author(s):

W. Lobato Paraense ◽

Lygia R. Corrêa

Keyword(s):

Schistosoma Mansoni ◽

Infection Rate ◽

Biomphalaria Glabrata ◽

Control Group ◽

Santa Catarina ◽

Potential Vector ◽

Daily Average ◽

Biomphalaria Straminea ◽

Species Inquirenda ◽

Definite Pattern

Susceptibily experiments were carried out with a Biomphalaria straminea-like planorbid snail (Biomphalaria aff. straminea, species inquirenda) from Espinillar, near Salto (Uruguay), in the area of the Salto Grande reservoir, exposed individually to 5 miracidia of Schistosoma mansoni (SJ2 and BH2 strains). Of 130 snails exposed to the SJ2 strain, originally infective to Biomphalaria tenagophila, 30 became infected (23%). The prepatent (precercaria) period ranged from 35 to 65 days. The cercarial output was irregular, following no definite pattern, varying from 138 to 76,075 per snail (daily average 4.3 to 447.5 and ending up with death. Three specimens that died, without having shed cercarie, on days 69 (2) and 80 after exposure to miracidia, had developing secondary sporocysts in their tissues, justifying the prospect of a longer precercarial period in these cases. In a control group of 120 B. teangophila, exposed to the SJ2 strain, 40 became infected, showing an infection rate (33.3%) not significantly different from that of the Espinillar snail (X [raised to the power of] 2 = 3.26). No cercarie were produced by any of the Espinilar snails exposed to miracidia of the BH2 strain, originally infective to Biomphalaria glabrata. Four specimens showed each a primary sporocyst in one tentacle, which disappeared between 15 and 25 days post-exposure, and two others died with immature, very slender sporocysts in their tissues on days 36 and 54. In a control group of 100 B. glabrata exposed to BH2 miracidia, 94 shed cercariae (94%) and 6 remained negative. Calculation of Frandsen's (1979a, b) TCP/100 index shows that "Espinillar Biomphalaria-SJ2 S. mansoni" is a vector-parasite "compatible" combination. Seeing that tenagophila-borne schistosomiasis is prevalent in Rio de Janeiro and São Paulo states and has recently spread sothwards to Santa Catarina state, and the range of B. tenagophila overlaps taht of the Espinillar Biomphalaria, the possibility of schistosomiais establishing itself in Uruguay, although not imminent, is not to be disregarded.

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Study of the snail intermediate hosts for Schistosoma mansoni on Itamaracá Island in northeast Brazil: spatial displacement of Biomphalaria glabrata by Biomphalaria straminea

Geospatial health ◽

10.4081/gh.2014.24 ◽

2014 ◽

Vol 8 (2) ◽

pp. 345 ◽

Cited By ~ 7

Author(s):

Constança S. Barbosa ◽

Verônica S. Barbosa ◽

Wheverton C. Nascimento ◽

Otavio S. Pieri ◽

Karina C. G. M. Araújo

Keyword(s):

Schistosoma Mansoni ◽

Biomphalaria Glabrata ◽

Northeast Brazil ◽

Intermediate Hosts ◽

Spatial Displacement ◽

Biomphalaria Straminea

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Behavioural responses to carboxylic and amino acids by Biomphalaria glabrata (say), the snail host of Schistosoma mansoni (Sambon), and other freshwater molluscs

Comparative Biochemistry and Physiology Part C Comparative Pharmacology ◽

10.1016/0742-8413(83)90012-9 ◽

1983 ◽

Vol 75 (1) ◽

pp. 57-76 ◽

Cited By ~ 4

Author(s):

J.D. Thomas ◽

J. Ofosu-Barko ◽

R.L. Patience

Keyword(s):

Amino Acids ◽

Schistosoma Mansoni ◽

Biomphalaria Glabrata ◽

Snail Host ◽

Behavioural Responses ◽

Freshwater Molluscs

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Genome size estimates for two important freshwater molluscs, the zebra mussel (Dreissena polymorpha) and the schistosomiasis vector snail (Biomphalaria glabrata)

Genome ◽

10.1139/g03-069 ◽

2003 ◽

Vol 46 (5) ◽

pp. 841-844 ◽

Cited By ~ 18

Author(s):

T Ryan Gregory

Keyword(s):

Image Analysis ◽

Schistosoma Mansoni ◽

Genome Size ◽

Zebra Mussel ◽

Dreissena Polymorpha ◽

Biomphalaria Glabrata ◽

Freshwater Snail ◽

Freshwater Molluscs ◽

Invading Species ◽

Suitable Target

The haploid genome sizes of two important molluscs were assessed by Feulgen image analysis densitometry. The genome size of the zebra mussel (Dreissena polymorpha), a prolific invader of North American lakes, was estimated to be 1C = 1.70 ± 0.03 pg, and that of the freshwater snail Biomphalaria glabrata, the predominant intermediate vector of the human parasite Schistosoma mansoni, was estimated at 0.95 ± 0.01 pg. These estimates will be important in future efforts in molluscan genomics, which at present lags far behind work being carried out with vertebrate and arthropod models. B. glabrata in particular, which has one of the smallest known gastropod genomes, is recommended as a highly suitable target for future genome sequencing.Key words: densitometry, DNA content, DNA sequencing, Feulgen, image analysis, Great Lakes, invading species, molluscs, Schistosoma mansoni.

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