Electrical conductivity and accelerated aging in amaranth (Amaranthus crueentus L.) seeds

Abstract: The objective of this study was to determine adequate conditions of water volume, seed quantity and immersion period to perform the electrical conductivity test, and to analyze the parameters of the accelerated aging test and its correlation with the the physiological potential of amaranth seeds. Five lots of amaranth seeds (Amaranthus cruentus), cultivar BRS Alegria, were used. The initial evaluation of the quality of these lots comprehended the following tests: water content, germination, first germination count, accelerated aging (traditional method, and modified versions, using unsaturated and saturated saline solution for the periods of 24, 48 and 72 h), electrical conductivity in different periods (2, 4, 6, 8 and 24 h), different quantities of water (25, 50 and 75 mL), different seed quantities (100 and 150), and field emergence. It could be concluded that the accelerated aging test at 41 °C, for 72 h, using unsaturated NaCl solution was efficient in the classification of amaranth seed lots in levels of vigor. The electrical conductivity test performed for 8 hours with 25 mL of water and 100 seeds was efficient in the classification of amaranth seeds as to their physiological potential.

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Tests for evaluating the physiological quality of pitaya seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n6sup2p4047 ◽

2015 ◽

Vol 36 (6Supl2) ◽

pp. 4047

Author(s):

Thiago Alberto Ortiz ◽

Aline Moritz ◽

Lúcia Sadayo Assari Takahashi ◽

Mariana Ragassi Urbano

Keyword(s):

Electrical Conductivity ◽

Pearson Correlation ◽

Accelerated Aging ◽

Seed Vigor ◽

Water Volume ◽

Germination Test ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

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Tests for evaluating the physiological quality of pitaya seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n6supl2p4047 ◽

2015 ◽

Vol 36 (6Supl2) ◽

pp. 4047

Author(s):

Thiago Alberto Ortiz ◽

Aline Moritz ◽

Lúcia Sadayo Assari Takahashi ◽

Mariana Ragassi Urbano

Keyword(s):

Electrical Conductivity ◽

Pearson Correlation ◽

Accelerated Aging ◽

Seed Vigor ◽

Water Volume ◽

Germination Test ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

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Physiological quality of popcorn seeds assessed by the accelerated aging test

Journal of Seed Science ◽

10.1590/2317-1545v40n4191101 ◽

2018 ◽

Vol 40 (4) ◽

pp. 428-434 ◽

Cited By ~ 2

Author(s):

Cristiane da Silva Rocha ◽

Hugo César Rodrigues Moreira Catão ◽

Franciele Caixeta ◽

Heloisa Karoline Kavan ◽

Talis Melo Claudino ◽

...

Keyword(s):

Electrical Conductivity ◽

Moisture Content ◽

Accelerated Aging ◽

Seed Moisture Content ◽

Randomized Design ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality ◽

Consistent Information

Abstract: Despite the insufficient internal supply of popcorn kernel cultivars, the Brazilian production is continuously increasing. Therefore, searching for tests that offer fast results and reliable information on the physiological potential of the seeds is very important. The objective of this study was to assess the efficiency of the accelerated aging test, by using different times and exposure temperatures, for the evaluation of the physiological quality of popcorn seed lots. Popcorn seeds from three lots of the hybrid AP 8203 were used. For the initial characterization of the lots, the following tests were conducted: seed moisture content, first and final germination count, and field emergence. The accelerated aging test was performed in a 3x4x2 factorial scheme. After aging, the seeds were tested for moisture content, germination, and electrical conductivity. The experiment was carried out in a completely randomized design. The characteristic vigor was affected during aging, as evidenced by the increase in the amount of leachate detected by the electrical conductivity test. Seed lot 3 was most vigorous in the aging test conducted for 48 h at 45 °C. The accelerated aging test at 42 ºC for 48 h provides consistent information to differentiate seed lots of popcorn.

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Accelerated aging test for forage pea seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n5p1819 ◽

2019 ◽

Vol 40 (5) ◽

pp. 1819

Author(s):

Carla Gomes Machado ◽

Givanildo Zildo Silva ◽

Silvia Sanielle Costa Oliveira ◽

Cibele Chalita Martins

Keyword(s):

Electrical Conductivity ◽

Potassium Chloride ◽

Seedling Emergence ◽

Accelerated Aging ◽

Cold Climate ◽

Accelerated Aging Test ◽

Aging Test ◽

Pea Seeds ◽

Pea Seed

Forage pea is an important annual, cold climate crop with high levels of essential amino acids in its seeds, which are lacking in the composition of cereal grains. Therefore, livestock farmers use its seeds as a nutritious source of forage. To plant fields of this forage, it is necessary to know the quality of the seeds, determined through germination and vigor tests. In the literature, studies on vigor tests for forage pea seeds are scarce. This study aimed to verify the efficiency of vigor tests, with emphasis on accelerated aging to evaluate the physiological quality of forage pea seeds in order to select lots for seedling emergence in the field. Using accelerated aging tests, 10 lots of forage pea were evaluated for moisture content, thousand-seed weight, emergence percentage, emergence first count, speed index of seedlings in the field, germination, germination first count, and electrical conductivity. The following variations of methodology were used: temperatures of 41 and 42 °C, exposure times of 48 and 72 h, and different relative humidity values in the aging atmosphere, using distilled water (100 % RH), saturated solution of potassium chloride (87 % RH), and sodium chloride (76 % RH). The tests of germination, emergence first count of seedlings in the field, emergence speed index, and electrical conductivity are promising to evaluate the vigor of forage pea seed lots. The accelerated aging test conducted in a solution saturated with potassium chloride at 42 °C for 48 h is adequate to estimate the vigor of forage pea seed lots, providing information similar to that of the emergence of seedlings in the field.

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Vigor testings for evaluating the physiological quality of chia seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n6supl2p2891 ◽

2019 ◽

Vol 40 (6Supl2) ◽

pp. 2891

Author(s):

Fernanda Brito Cardoso ◽

Carlos Henrique Queiroz Rego ◽

Izabela Cristina de Oliveira ◽

Alan Mario Zuffo ◽

Ana Carina da Silva Cândido ◽

...

Keyword(s):

Electrical Conductivity ◽

Traditional Method ◽

Seedling Emergence ◽

Accelerated Aging ◽

Distilled Water ◽

Nacl Solution ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Chia (Salvia hispanica L.) is a plant native to central and western Mexico and northern Guatemala belonging to the Lamiaceae family, well known for its seeds containing antioxidants, dietary fibers, and ?-linolenic acid, which helps to prevent different diseases, such as diabetes, obesity, cancer and Alzheimer. In Brazil, there is not much information about the crop, mainly about the physiological quality of its seeds. Therefore, the objective was to establish vigor testing methods to evaluate the physiological quality of chia seeds. Five seed lots were used, in which germination tests, seedling emergence, emergence velocity index and variations in the electrical conductivity test were carried out. The volume of distilled water was 25 and 50 mL, the number of seeds was 50 and 100, the temperature was 20, 25 and 30 °C, the soaking period was 1, 2, 4, 6, 12, 18 and 24 hours and variations in there was accelerated aging test by the traditional method and saturated NaCl solution, being periods of aging as 24, 48, 72 and 96 hours and temperatures of 38, 41 and 44 °C. It is concluded that both the electrical conductivity test and the accelerated aging test are efficient in evaluating the physiological quality of chia seeds. The electrical conductivity test must be performed with 100 seeds in 50 mL of distilled water, for four hours soaking at 25 or 30 °C; and the accelerated aging test should be performed at 44 °C for 24 hours by the traditional method or 41 °C for 24 hours using saturated NaCl solution.

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Electrical conductivity for seed vigor test in sorghum (Sorghum bicolor)

Cell Biology and Development ◽

10.13057/cellbioldev/v010102 ◽

2017 ◽

Vol 1 (1) ◽

pp. 6-12 ◽

Cited By ~ 2

Author(s):

KHODRATIEN FATONAH ◽

IRFAN SULIANSYAH ◽

NALWIDA ROZEN

Keyword(s):

Electrical Conductivity ◽

Accelerated Aging ◽

Seed Vigor ◽

Test Method ◽

Water Volume ◽

Conductivity Method ◽

Randomized Design ◽

Accelerated Aging Test ◽

Aging Test ◽

Potassium Leakage

Fatonah K, Suliansyah I, Rozen N. 2017. Electrical conductivity for seed vigor test in sorghum (Sorghum bicolor). Cell Biol Dev 1: 6-12. The objectives of this study were to obtain electrical conductivity test method for seed vigor test in sorghum, to recognize relationship between electrical conductivity test and potassium leakage, and to recognize relationship between electrical conductivity test and other variable on seed vigor. This study have two step experiments. The objective of Experiment I was to determine accurate combinations of water volume and amount seed of the electrical conductivity test for seed vigor test in sorghum. Completely Randomized Design of 15 seed lots with 3 replications were used to determine electrical conductivity method with different vigor. Experiment II were to test electrical conductivity method of 21 seed lots of sorghum without accelerated aging test and 21 seed lots of sorghum were given accelerated aging test. Completely Randomized Design of 21 seed lots with 3 replications were used in Experiment II. The variable were observed : standard germination, field emergence, speed of germination, first count, conductivity, potassium leakage and eight combination conductivity method of water volume (50, 100, 150 and 200 ml) and amount of seed sorghum (50 and 75 seed count). The result of this experiments showed that electrical conductivity test method with 150 ml water volume and 75 seed count was accurate and suitable for sorghum seed vigor test; electrical conductivity test showed positive correlation with potassium leakage;and electrical conductivity test can be used for seed vigor test in sorghum and provided the potential of physiological seed were shown through : standard germination test, field emergence test, first count test and speed of germination with negative correlation.

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ACCELERATED AGING AND ELECTRICAL CONDUCTIVITY TESTS IN CRAMBE SEEDS

Ciência e Agrotecnologia ◽

10.1590/s1413-70542015000100001 ◽

2015 ◽

Vol 39 (1) ◽

pp. 7-14 ◽

Cited By ~ 4

Author(s):

Juliana Joice Pereira Lima ◽

Marcella Nunes de Freitas ◽

Renato Mendes Guimarães ◽

Antônio Rodrigues Vieira ◽

Maria Alice Bento Ávila

Keyword(s):

Electrical Conductivity ◽

Chloride Solution ◽

Accelerated Aging ◽

Germination Test ◽

Crambe Seed ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

The aim of this study was to adapt the methodology of the accelerated aging and electrical conductivity tests for determination of physiological potential in crambe seeds. Six seed lots of crambe (cv. FMS Brilhante) were subjected to determination of moisture content, germination test, first count germination, emergence, and emergence speed index. For the accelerated aging test, the traditional methodology was used with water, and with a saturated potassium chloride and sodium chloride solution in three periods of exposure (24, 48, and 72 hours) at 41°C; the electrical conductivity test was performed with four pre-soaking treatments (0, 2, 4, and 8 hours) and four soaking periods (4, 8, 16, and 24 hours) at 25°C. The accelerated aging test with water for 72 hours and the electrical conductivity test with 2 hours of pre-soaking and assessment after 16 hours were effective for classification of the crambe seed lots in regard to physiological quality.

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Methodological adjustment of the accelerated aging test to evaluate the vigor of chia seeds

Journal of Seed Science ◽

10.1590/2317-1545v40n2188348 ◽

2018 ◽

Vol 40 (2) ◽

pp. 173-178 ◽

Cited By ~ 1

Author(s):

Aline Klug Radke ◽

Fernanda da Motta Xavier ◽

Paulo Eduardo Rocha Eberhardt ◽

Francisco Amaral Villela ◽

Géri Eduardo Meneghello

Keyword(s):

Saline Solution ◽

Standardized Test ◽

Seedling Emergence ◽

Accelerated Aging ◽

Accelerated Aging Test ◽

Chia Seed ◽

Aging Test ◽

Conventional Test

Abstract: Chia cultivation is expanding in Brazil, but there is still no standardized test to evaluate the quality of the seeds. Therefore, vigor tests that expose seeds to stress conditions must be standardized. This study aimed to establish the methodology for the accelerated aging test in chia seeds. Six chia seed lots with similar germination behavior were used. They were submitted to germination, first germination count, seedling emergence, emergence speed index and accelerated aging tests. Aging periods of 24, 48, and 72 hours were used in the conventional methodology, and also with the use of unsaturated and saturated sodium chloride (NaCl) solutions. The results obtained from the accelerated aging tests revealed tendencies for lot separation similar to the classification of chia seed lots in relation to the physiological potential obtained in the preliminary quality tests. The accelerated aging test can be used to evaluate the physiological potential of chia seeds by using water or unsaturated saline solution. In the conventional test (water), the seeds should be exposed for 24 hours. When using unsaturated saline solution, the exposure time must be of 48 hours.

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Genetic diversity for physiological quality of seeds from corn (Zea mays L.) intervarietal crossbreeds

Journal of Seed Science ◽

10.1590/s2317-15372013000400006 ◽

2013 ◽

Vol 35 (4) ◽

pp. 449-456 ◽

Cited By ~ 4

Author(s):

Daniele Nerling ◽

Cileide Maria Medeiros Coelho ◽

Rubens Onofre Nodari

Keyword(s):

Genetic Diversity ◽

Zea Mays L ◽

Accelerated Aging ◽

Genetic Contribution ◽

Percentage Germination ◽

Canonical Variables ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

The physiological quality is based on the genotype and may be accompanied since the first stages of selection through the study of genetic diversity. The purpose of this study was to evaluate the parental genetic contribution in the seed physiological quality in corn intervarietal crossbreeds. The intervarietal crossbreeds were performed using landraces genotypes, open pollinated varieties and commercial hybrids. The seeds obtained from the crossbreed, their equivalents and parentals were evaluated regarding their water percentage, germination, germination speed rate, accelerated aging, field emergence and electrical conductivity. The seeds obtained in the crossbreed are viable and vivid with heterosis when compared with the parentals. The accelerated aging test was more effective for selecting the strongest genotypes. The canonical variables were useful to group the genotypes that presented similar physiological characteristics. The genotypes MPA 01 x Pixurum 05, MPA 01 x AS1565, MPA 01 x SJC5886, SJC5886 x Pixurum 05 and Fundacep 35 x SCS 154 Fortuna were the best crossbreeds regarding the production of better physiological quality seeds.

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