scholarly journals Vigor testings for evaluating the physiological quality of chia seeds

2019 ◽  
Vol 40 (6Supl2) ◽  
pp. 2891
Author(s):  
Fernanda Brito Cardoso ◽  
Carlos Henrique Queiroz Rego ◽  
Izabela Cristina de Oliveira ◽  
Alan Mario Zuffo ◽  
Ana Carina da Silva Cândido ◽  
...  

Chia (Salvia hispanica L.) is a plant native to central and western Mexico and northern Guatemala belonging to the Lamiaceae family, well known for its seeds containing antioxidants, dietary fibers, and ?-linolenic acid, which helps to prevent different diseases, such as diabetes, obesity, cancer and Alzheimer. In Brazil, there is not much information about the crop, mainly about the physiological quality of its seeds. Therefore, the objective was to establish vigor testing methods to evaluate the physiological quality of chia seeds. Five seed lots were used, in which germination tests, seedling emergence, emergence velocity index and variations in the electrical conductivity test were carried out. The volume of distilled water was 25 and 50 mL, the number of seeds was 50 and 100, the temperature was 20, 25 and 30 °C, the soaking period was 1, 2, 4, 6, 12, 18 and 24 hours and variations in there was accelerated aging test by the traditional method and saturated NaCl solution, being periods of aging as 24, 48, 72 and 96 hours and temperatures of 38, 41 and 44 °C. It is concluded that both the electrical conductivity test and the accelerated aging test are efficient in evaluating the physiological quality of chia seeds. The electrical conductivity test must be performed with 100 seeds in 50 mL of distilled water, for four hours soaking at 25 or 30 °C; and the accelerated aging test should be performed at 44 °C for 24 hours by the traditional method or 41 °C for 24 hours using saturated NaCl solution.

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4047
Author(s):  
Thiago Alberto Ortiz ◽  
Aline Moritz ◽  
Lúcia Sadayo Assari Takahashi ◽  
Mariana Ragassi Urbano

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.


2018 ◽  
Vol 40 (4) ◽  
pp. 428-434 ◽  
Author(s):  
Cristiane da Silva Rocha ◽  
Hugo César Rodrigues Moreira Catão ◽  
Franciele Caixeta ◽  
Heloisa Karoline Kavan ◽  
Talis Melo Claudino ◽  
...  

Abstract: Despite the insufficient internal supply of popcorn kernel cultivars, the Brazilian production is continuously increasing. Therefore, searching for tests that offer fast results and reliable information on the physiological potential of the seeds is very important. The objective of this study was to assess the efficiency of the accelerated aging test, by using different times and exposure temperatures, for the evaluation of the physiological quality of popcorn seed lots. Popcorn seeds from three lots of the hybrid AP 8203 were used. For the initial characterization of the lots, the following tests were conducted: seed moisture content, first and final germination count, and field emergence. The accelerated aging test was performed in a 3x4x2 factorial scheme. After aging, the seeds were tested for moisture content, germination, and electrical conductivity. The experiment was carried out in a completely randomized design. The characteristic vigor was affected during aging, as evidenced by the increase in the amount of leachate detected by the electrical conductivity test. Seed lot 3 was most vigorous in the aging test conducted for 48 h at 45 °C. The accelerated aging test at 42 ºC for 48 h provides consistent information to differentiate seed lots of popcorn.


2019 ◽  
Vol 40 (5) ◽  
pp. 1819
Author(s):  
Carla Gomes Machado ◽  
Givanildo Zildo Silva ◽  
Silvia Sanielle Costa Oliveira ◽  
Cibele Chalita Martins

Forage pea is an important annual, cold climate crop with high levels of essential amino acids in its seeds, which are lacking in the composition of cereal grains. Therefore, livestock farmers use its seeds as a nutritious source of forage. To plant fields of this forage, it is necessary to know the quality of the seeds, determined through germination and vigor tests. In the literature, studies on vigor tests for forage pea seeds are scarce. This study aimed to verify the efficiency of vigor tests, with emphasis on accelerated aging to evaluate the physiological quality of forage pea seeds in order to select lots for seedling emergence in the field. Using accelerated aging tests, 10 lots of forage pea were evaluated for moisture content, thousand-seed weight, emergence percentage, emergence first count, speed index of seedlings in the field, germination, germination first count, and electrical conductivity. The following variations of methodology were used: temperatures of 41 and 42 °C, exposure times of 48 and 72 h, and different relative humidity values in the aging atmosphere, using distilled water (100 % RH), saturated solution of potassium chloride (87 % RH), and sodium chloride (76 % RH). The tests of germination, emergence first count of seedlings in the field, emergence speed index, and electrical conductivity are promising to evaluate the vigor of forage pea seed lots. The accelerated aging test conducted in a solution saturated with potassium chloride at 42 °C for 48 h is adequate to estimate the vigor of forage pea seed lots, providing information similar to that of the emergence of seedlings in the field.


2013 ◽  
Vol 35 (2) ◽  
pp. 147-152 ◽  
Author(s):  
Fábio Oliveira Diniz ◽  
Múcio Silva Reis ◽  
Luiz Antônio dos Santos Dias ◽  
Eduardo Fontes Araújo ◽  
Tuneo Sediyama ◽  
...  

With the aim of studying the relationship between the physiological quality of soybean seeds submitted to three harvest times and seedling emergence in the field, seeds of cultivars UFV-16, Splendor, Vencedora, Confiança, UFV-18, UFV-TN 105, Garantia and Celeste were harvested at stages R8, R8+15 and R8+30 days. The following tests were performed: accelerated aging, electrical conductivity, emergence in sand, emergence speed index and seedling emergence in the field. Germination and vigor were reduced in seeds harvested at 30 days after stage R8 in all the tests, except for the accelerated aging test, which showed a decline in vigor seeds harvested with a delay of 15 days. Seeds of cultivars UFV-16, Confiança and Garantia showed lower physiological quality in most tests, while the seeds of cultivar Celeste had higher physiological quality than the others. There was similarity between the test results of seed quality and seedling emergence in the field.


2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4047
Author(s):  
Thiago Alberto Ortiz ◽  
Aline Moritz ◽  
Lúcia Sadayo Assari Takahashi ◽  
Mariana Ragassi Urbano

<p>Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe <em>Hylocereus undatus </em>fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p &lt; 0.05). The germination test for <em>H. undatus </em>seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.</p>


2003 ◽  
Vol 60 (1) ◽  
pp. 77-82 ◽  
Author(s):  
Salvador Barros Torres ◽  
Julio Marcos Filho

Accelerated aging is one of the most useful tests used for the evaluation of seed vigor but it is seldomly used to test melon (Cucumis melo L.) seeds. The objective this research was to compare different procedures of the accelerated aging test to evaluate the physiological quality of melon seeds and the efficiency of using saturated salt solution for the control of water uptake by seeds. Five seed lots each of the hybrids AF-646 and AF-682 were tested for germination, seedling emergence, traditional accelerated aging (periods 0f 48, 72 and 96 hours, at 38 or 41°C) and salt saturated accelerated aging. The accelerated aging test (traditional procedure and with salt solution) for 72h and 96h, at 38 or 41°C was sensitive to detect differences in the physiological quality of the seeds. It was also observed that the seed water content after salt saturated accelerated aging was lower and more uniform, thus presenting advantagens in relation to the traditional procedure.


2021 ◽  
Vol 43 ◽  
Author(s):  
Rayssa Fernanda dos Santos ◽  
Henrique Fabrício Placido ◽  
Leonardo Lemes Bosche ◽  
Hugo Zeni Neto ◽  
Fernando Augusto Henning ◽  
...  

Abstract: This work evaluated the efficiency of accelerated aging test to determine the physiological potential in treated soybean seeds with fungicides and insecticides, in addition to analyzing the concordance between the results obtained by different accelerated aging methods with the actual results of the physiological quality in seeds after the storage. The study was divided into two experiments, both with entirely randomized delineation, with four replications. In the first experiment, seed germination was evaluated after imposing some treatments that were arranged in a 3 x 2 x 3 factorial scheme: seed treatment (imidacloprid + thiodicarb; fipronil + thiophanate methyl + pyraclostrobin; no treatment); vigor levels (high; low); and accelerated aging (traditional; saturated NaCl solution; no aging). In the second experiment, seedling emergence was evaluated after applying some treatments that were arranged in a 3 x 2 x 2 factorial scheme: seed treatment (imidacloprid + thiodicarb; fipronil + thiophanate methyl + pyraclostrobin; no treatment); vigor levels (high; low); storage time (0 and 135 days). The accelerated aging methods were efficient to determine the physiological potential of the seeds, in which, the NaCl method presented better results. The physiological potential of soybean seeds decreased throughout storage and the application of chemicals in seed treatment impaired the germination and emergence percentages, regardless of the active ingredient used. The accelerated aging method with NaCl presents greater agreement with the actual storage results.


2019 ◽  
Vol 21 (3) ◽  
pp. 166-182
Author(s):  
Andréa Bicca Noguez Martins ◽  
Ivan Ricardo Carvalho ◽  
Giordano Gelain Conte ◽  
Anna dos Santos Suñe ◽  
Bruna Barreto Dos Reis ◽  
...  

The physiological quality of seeds is commonly characterized by the germination and vigor, which can be defined as the sum of attributes that confers the potential for seeds to germinate, rapidly emerge and result in normal seedlings under a wide range of environmental conditions. The purpose of the study was to test alternative methodologies for the accelerated aging test to evaluate the physiological potential of poppy seeds. Samples obtained from four batches were subjected to germination test at a temperature of 20 °C, traditional accelerated aging and accelerated aging diluted with saline solution (11 g of NaCl  in 100 mL-1  of water) using three temperatures, 41 °C, 42 ºC and 43 ºC , for periods of 12 and 24 hours. The percentage of germination, the rate of emergence velocity, performed together with the emergence speed and the percentage of seedling emergence test were evaluated. It is concluded that the methodology of traditional accelerated aging with temperature of 43 °C and period of 12h shows to be promising for the verification of the physiological quality of poppy seeds.


2013 ◽  
Vol 35 (4) ◽  
pp. 449-456 ◽  
Author(s):  
Daniele Nerling ◽  
Cileide Maria Medeiros Coelho ◽  
Rubens Onofre Nodari

The physiological quality is based on the genotype and may be accompanied since the first stages of selection through the study of genetic diversity. The purpose of this study was to evaluate the parental genetic contribution in the seed physiological quality in corn intervarietal crossbreeds. The intervarietal crossbreeds were performed using landraces genotypes, open pollinated varieties and commercial hybrids. The seeds obtained from the crossbreed, their equivalents and parentals were evaluated regarding their water percentage, germination, germination speed rate, accelerated aging, field emergence and electrical conductivity. The seeds obtained in the crossbreed are viable and vivid with heterosis when compared with the parentals. The accelerated aging test was more effective for selecting the strongest genotypes. The canonical variables were useful to group the genotypes that presented similar physiological characteristics. The genotypes MPA 01 x Pixurum 05, MPA 01 x AS1565, MPA 01 x SJC5886, SJC5886 x Pixurum 05 and Fundacep 35 x SCS 154 Fortuna were the best crossbreeds regarding the production of better physiological quality seeds.


2018 ◽  
Vol 40 (1) ◽  
pp. 44-51
Author(s):  
Thaís D’Avila Rosa ◽  
Ariele Paula Nadal ◽  
Henrique Roberto Maldaner ◽  
Vanessa Nogueira Soares ◽  
Gizele Ingrid Gadotti ◽  
...  

Abstract: The objective of this study was to determine adequate conditions of water volume, seed quantity and immersion period to perform the electrical conductivity test, and to analyze the parameters of the accelerated aging test and its correlation with the the physiological potential of amaranth seeds. Five lots of amaranth seeds (Amaranthus cruentus), cultivar BRS Alegria, were used. The initial evaluation of the quality of these lots comprehended the following tests: water content, germination, first germination count, accelerated aging (traditional method, and modified versions, using unsaturated and saturated saline solution for the periods of 24, 48 and 72 h), electrical conductivity in different periods (2, 4, 6, 8 and 24 h), different quantities of water (25, 50 and 75 mL), different seed quantities (100 and 150), and field emergence. It could be concluded that the accelerated aging test at 41 °C, for 72 h, using unsaturated NaCl solution was efficient in the classification of amaranth seed lots in levels of vigor. The electrical conductivity test performed for 8 hours with 25 mL of water and 100 seeds was efficient in the classification of amaranth seeds as to their physiological potential.


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