ACCELERATED AGING AND ELECTRICAL CONDUCTIVITY TESTS IN CRAMBE SEEDS

The aim of this study was to adapt the methodology of the accelerated aging and electrical conductivity tests for determination of physiological potential in crambe seeds. Six seed lots of crambe (cv. FMS Brilhante) were subjected to determination of moisture content, germination test, first count germination, emergence, and emergence speed index. For the accelerated aging test, the traditional methodology was used with water, and with a saturated potassium chloride and sodium chloride solution in three periods of exposure (24, 48, and 72 hours) at 41°C; the electrical conductivity test was performed with four pre-soaking treatments (0, 2, 4, and 8 hours) and four soaking periods (4, 8, 16, and 24 hours) at 25°C. The accelerated aging test with water for 72 hours and the electrical conductivity test with 2 hours of pre-soaking and assessment after 16 hours were effective for classification of the crambe seed lots in regard to physiological quality.

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Tests for evaluating the physiological quality of pitaya seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n6sup2p4047 ◽

2015 ◽

Vol 36 (6Supl2) ◽

pp. 4047

Author(s):

Thiago Alberto Ortiz ◽

Aline Moritz ◽

Lúcia Sadayo Assari Takahashi ◽

Mariana Ragassi Urbano

Keyword(s):

Electrical Conductivity ◽

Pearson Correlation ◽

Accelerated Aging ◽

Seed Vigor ◽

Water Volume ◽

Germination Test ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

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Tests for evaluating the physiological quality of pitaya seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n6supl2p4047 ◽

2015 ◽

Vol 36 (6Supl2) ◽

pp. 4047

Author(s):

Thiago Alberto Ortiz ◽

Aline Moritz ◽

Lúcia Sadayo Assari Takahashi ◽

Mariana Ragassi Urbano

Keyword(s):

Electrical Conductivity ◽

Pearson Correlation ◽

Accelerated Aging ◽

Seed Vigor ◽

Water Volume ◽

Germination Test ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

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Electrical conductivity and accelerated aging in amaranth (Amaranthus crueentus L.) seeds

Journal of Seed Science ◽

10.1590/2317-1545v40n1183116 ◽

2018 ◽

Vol 40 (1) ◽

pp. 44-51

Author(s):

Thaís D’Avila Rosa ◽

Ariele Paula Nadal ◽

Henrique Roberto Maldaner ◽

Vanessa Nogueira Soares ◽

Gizele Ingrid Gadotti ◽

...

Keyword(s):

Electrical Conductivity ◽

Accelerated Aging ◽

Water Volume ◽

Amaranthus Cruentus ◽

Accelerated Aging Test ◽

Aging Test ◽

Immersion Period ◽

Amaranth Seed

Abstract: The objective of this study was to determine adequate conditions of water volume, seed quantity and immersion period to perform the electrical conductivity test, and to analyze the parameters of the accelerated aging test and its correlation with the the physiological potential of amaranth seeds. Five lots of amaranth seeds (Amaranthus cruentus), cultivar BRS Alegria, were used. The initial evaluation of the quality of these lots comprehended the following tests: water content, germination, first germination count, accelerated aging (traditional method, and modified versions, using unsaturated and saturated saline solution for the periods of 24, 48 and 72 h), electrical conductivity in different periods (2, 4, 6, 8 and 24 h), different quantities of water (25, 50 and 75 mL), different seed quantities (100 and 150), and field emergence. It could be concluded that the accelerated aging test at 41 °C, for 72 h, using unsaturated NaCl solution was efficient in the classification of amaranth seed lots in levels of vigor. The electrical conductivity test performed for 8 hours with 25 mL of water and 100 seeds was efficient in the classification of amaranth seeds as to their physiological potential.

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Reduction of the Forage Seeds Physiological Quality by the Contact with Fertilizer in the Crop-Livestock Integration

Journal of Experimental Agriculture International ◽

10.9734/jeai/2019/v34i630190 ◽

2019 ◽

pp. 1-11

Author(s):

Anne Caroline Dallabrida Avelino ◽

Dayana Aparecida De Faria ◽

Carlos Eduardo Avelino Cabral ◽

Joadil Gonçalves De Abreu ◽

Elisangela Clarete Camili ◽

...

Keyword(s):

Electrical Conductivity ◽

Contact Time ◽

Accelerated Aging ◽

Germination Percentage ◽

Randomized Design ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality ◽

Completely Randomized Design ◽

Seeds Germination

The aim of this study was to evaluate the effects of Brachiaria seed contact time with 05-25-15 fertilizer (N-P2O5-K2O) on the seed’s physiological quality. The experimental design was a completely randomized design with four replicates. Treatments were arranged in a 5x4 factorial, being: five seeds contact times with fertilizer (0, 24, 48, 72 and 96 hours); four forages: Marandu palisadegrass, Piatã palisadegrass, Xaraés palisadegrass and Ruziziensis grass. The performed tests were: water content, germination (first count and germination percentage), tetrazolium (viability), electrical conductivity, accelerated aging, sand emergency and emergence speed index. Contact time of 05-25-15 fertilizer with Brachiaria seeds reduces linearly: Germination, emergence and emergence speed index of Brachiaria seeds; Piatã and Xaraés palisadegrass seeds viability; vigor (electrical conductivity), except in Ruziziensis grass seeds; and seeds germination of Piatã palisadegrass submitted to accelerated aging test (vigor). Piatã palisadegrass is the least recommended, among the analyzed grasses, for intercropping in simultaneous sowing.

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Improving the method for determining the physiological and sanitary potential of Gherkin seeds

Bioscience Journal ◽

10.14393/bj-v37n0a2021-54193 ◽

2021 ◽

Vol 37 ◽

pp. e37087

Author(s):

Hugo Cesar Rodrigues Moreira Catão ◽

Ítala Menegon Castilho ◽

Franciele Caixeta ◽

Nilvanira Donizete Tebaldi ◽

Pâmela Gomes Nakada Freitas

Keyword(s):

Water Content ◽

Saline Solution ◽

Seed Quality ◽

Accelerated Aging ◽

Analysis Method ◽

Germination Test ◽

Seed Technology ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Gherkin seeds usually show irregular physiological quality. Seed production requires fast and reliable tests to evaluate seed quality. Germination test is considered a recognized analysis method; however, seed technology has pursuit the improvement of vigor tests aiming the evaluation of seed’s physiological potential. Thus, the objective of this work was to evaluate procedures to perform the test of accelerated aging and determine the physiological and sanitary potential of gherkin seeds. Four seed lots of cultivar Liso Calcuta were used in the study. To evaluate the initial physiological quality the water content was determined and germination and emergence tests, as well as indices of germination speed and emergence speed were used. The accelerated aging test was performed as traditionally and with saturated saline solution, with 48, 72 and 96 hours, at temperatures of 41oC and 45oC. After aging, the water content was determined, and seeds’ germination and sanity tests were performed. The experiment was set under a completely random design in factorial 4x3x2 (lots x aging periods x temperatures). The standard accelerated aging test and the test with saturated saline solution at 41oC for 96 hours were efficient to evaluate the vigor of gherkin seeds. Saturated saline solution provides uniform water absorption and deterioration in gherkin seeds, allowing to discriminate seed lots in different vigor levels. The salinity test after accelerated aging with saline solution reduces the incidence of some fungi.

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Seed vigor of Piptadenia moniliformis Benth. subjected to thermal stress

Acta Scientiarum Biological Sciences ◽

10.4025/actascibiolsci.v42i1.51287 ◽

2020 ◽

Vol 42 ◽

pp. e51287

Author(s):

Joyce Naiara da Silva ◽

Monalisa Alves Diniz da Silva

Keyword(s):

Electrical Conductivity ◽

Sulfuric Acid ◽

Accelerated Aging ◽

Seed Vigor ◽

Saturated Solution ◽

Aging Period ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality ◽

Concentrated Sulfuric Acid

The objective of this work is to evaluate the influence of accelerated aging test at 41ºC on the germination and seed vigor of Piptadenia moniliformis Benth. Two experiments were conducted. Experiment 1: seeds were submitted to dormancy overcoming treatments. They were immersed into sulfuric acid for 0 (intact seeds), 5, 10, 15, 20 and 25 minutes. Experiment 2: two methods were evaluated for the accelerated aging test at 41ºC, that is, the traditional method and the saturated NaCl solution method with exposure periods of 0, 24, 48, 72 and 96 hours. After each treatment, the seeds were subjected to electrical conductivity and germination tests. Seeds overcame their dormancy when immersed into concentrated sulfuric acid for 5, 10, 15, 20 or 25 minutes, but the germination was faster when immersed for 20 minutes. The different methods for the accelerated aging test, decreased the germination potential and seed vigor of P. moniliformis after 24 hours of exposure. Regarding the method used, the saturated solution provided, in general, the best results since the seed water content was lower, thus reducing the proliferation of fungi. The electrical conductivity test showed that, by increasing the aging period regardless of the method, there is an increase in seed deterioration, corroborating with germination results, which showed that the correlation was moderate and negative and indicated that the higher the conductivity, the lower the percentage and the germination speed index. The immersion of seeds of P. moniliformis into concentrated sulfuric acid for 20 minutes provides a fast and increased germination. The methods for accelerated aging at 41ºC, traditional and saturated solution, can be used as vigor tests to evaluate the physiological quality of seeds.

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Physiological quality of popcorn seeds assessed by the accelerated aging test

Journal of Seed Science ◽

10.1590/2317-1545v40n4191101 ◽

2018 ◽

Vol 40 (4) ◽

pp. 428-434 ◽

Cited By ~ 2

Author(s):

Cristiane da Silva Rocha ◽

Hugo César Rodrigues Moreira Catão ◽

Franciele Caixeta ◽

Heloisa Karoline Kavan ◽

Talis Melo Claudino ◽

...

Keyword(s):

Electrical Conductivity ◽

Moisture Content ◽

Accelerated Aging ◽

Seed Moisture Content ◽

Randomized Design ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality ◽

Consistent Information

Abstract: Despite the insufficient internal supply of popcorn kernel cultivars, the Brazilian production is continuously increasing. Therefore, searching for tests that offer fast results and reliable information on the physiological potential of the seeds is very important. The objective of this study was to assess the efficiency of the accelerated aging test, by using different times and exposure temperatures, for the evaluation of the physiological quality of popcorn seed lots. Popcorn seeds from three lots of the hybrid AP 8203 were used. For the initial characterization of the lots, the following tests were conducted: seed moisture content, first and final germination count, and field emergence. The accelerated aging test was performed in a 3x4x2 factorial scheme. After aging, the seeds were tested for moisture content, germination, and electrical conductivity. The experiment was carried out in a completely randomized design. The characteristic vigor was affected during aging, as evidenced by the increase in the amount of leachate detected by the electrical conductivity test. Seed lot 3 was most vigorous in the aging test conducted for 48 h at 45 °C. The accelerated aging test at 42 ºC for 48 h provides consistent information to differentiate seed lots of popcorn.

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SUITABILITY OF ELECTRICAL CONDUCTIVITY TESTING AND ACCELERATED AGING FOR EVALUATING THE VIGOR OFCITHAREXYLUM MYRIANTHUM CHAM. DIASPORES

FLORESTA ◽

10.5380/rf.v49i4.57765 ◽

2019 ◽

Vol 49 (4) ◽

pp. 701

Author(s):

Ana Paula Bazzanella ◽

Gabriele Larissa Mathias ◽

Henrique Roberto Gattermann Mittelstaedt ◽

Maiara Iadwizak Ribeiro ◽

Andréa Maria Teixeira Forte ◽

...

Keyword(s):

Electrical Conductivity ◽

Accelerated Aging ◽

Germination Test ◽

High Demand ◽

Tukey Test ◽

Aging Period ◽

Accelerated Aging Test ◽

Aging Test ◽

Randomized Experimental Design ◽

Native Tree Species

The Atlantic Forest is one of the most threatened Brazilian biomes by anthropic action. Thus, the interest inrecovering degraded areas through the use of native tree species has increased, causing a high demand in the use of vigorous seeds. This research aimed at evaluating the suitabilityof the electrical conductivity and accelerated aging tests to evaluate the vigor of C. myrianthum diaspores. For the adequacy of the electrical conductivity test, a completely randomized experimental design (CRD) was used in a 2 x 3 x9 (number of diaspores x volume of water x imbibition time)at a constant temperature of 25 °C. The procedure for the accelerated aging test was conducted at 40 ºC for 0 (no aging), 24, 48 and 72 hours. ACRDwas also used for the accelerated aging test, totaling 4 treatments. The results were submitted to analysis of variance (ANOVA) and the means were compared by the Tukey test at 5% probability. It is concluded that the electrical conductivity test for C. myrianthum diaspores can be conducted with 25 diaspores soaked with 50 mL of deionized water for 96 hours, thus replacing the germination test with the advantage of being carried out in a shorter period of time. Moreover,the 24 and 48 hour accelerated aging period is adequate to discriminate the vigor of C. myrianthum diaspores, with GSI and MGT being the most sensitive variables.

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Parameters of the Accelerated Aging Test for the Determination of Tobacco (Nicotiana tabacum L.) Seed Vigor

Journal of Agricultural Studies ◽

10.5296/jas.v8i1.15828 ◽

2020 ◽

Vol 8 (1) ◽

pp. 247

Author(s):

Luis Henrique Konzen ◽

Daniele Brandstetter Rodrigues ◽

Alcimar Spindola Mazon ◽

William Aldrei Guareschi ◽

Jerffeson Araujo Cavalcante ◽

...

Keyword(s):

Saline Solution ◽

Seedling Emergence ◽

Accelerated Aging ◽

Exposure Period ◽

Seed Vigor ◽

Germination Test ◽

Accelerated Aging Test ◽

Tobacco Seeds ◽

Aging Test

Vigor tests are very important to obtain additional information to the standard germination test and can assist in decision-making. The aim of this work was to evaluate accelerated aging test methodologies for the determination of vigor in tobacco seeds. For the study, ten seed lots of tobacco were used. The initial quality of the tobacco seeds was determined through the germination test, first count of germination, germination speed index, seedling emergence at 7, 14 and 21 days after sowing, emergence speed index and the accelerated aging with water performed as proposed by the Association of Official Seed Analysts. After the determination of seed initial quality, the accelerated aging test was studied in the following methods: accelerated aging with water, saturated saline solution (40g NaCl 100mL-1 water) and unsaturated saline solution (11g NaCl 100mL-1 water), submitted to the temperatures of 45 and 41°C, for the exposure periods of 24, 48 and 72 hours. According to the results obtained, the accelerated aging test with water conducted under a temperature of 45°C combined with a 24-hour exposure period is shown as adequate for evaluating the vigor of tobacco seeds.

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Vigor testings for evaluating the physiological quality of chia seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n6supl2p2891 ◽

2019 ◽

Vol 40 (6Supl2) ◽

pp. 2891

Author(s):

Fernanda Brito Cardoso ◽

Carlos Henrique Queiroz Rego ◽

Izabela Cristina de Oliveira ◽

Alan Mario Zuffo ◽

Ana Carina da Silva Cândido ◽

...

Keyword(s):

Electrical Conductivity ◽

Traditional Method ◽

Seedling Emergence ◽

Accelerated Aging ◽

Distilled Water ◽

Nacl Solution ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Chia (Salvia hispanica L.) is a plant native to central and western Mexico and northern Guatemala belonging to the Lamiaceae family, well known for its seeds containing antioxidants, dietary fibers, and ?-linolenic acid, which helps to prevent different diseases, such as diabetes, obesity, cancer and Alzheimer. In Brazil, there is not much information about the crop, mainly about the physiological quality of its seeds. Therefore, the objective was to establish vigor testing methods to evaluate the physiological quality of chia seeds. Five seed lots were used, in which germination tests, seedling emergence, emergence velocity index and variations in the electrical conductivity test were carried out. The volume of distilled water was 25 and 50 mL, the number of seeds was 50 and 100, the temperature was 20, 25 and 30 °C, the soaking period was 1, 2, 4, 6, 12, 18 and 24 hours and variations in there was accelerated aging test by the traditional method and saturated NaCl solution, being periods of aging as 24, 48, 72 and 96 hours and temperatures of 38, 41 and 44 °C. It is concluded that both the electrical conductivity test and the accelerated aging test are efficient in evaluating the physiological quality of chia seeds. The electrical conductivity test must be performed with 100 seeds in 50 mL of distilled water, for four hours soaking at 25 or 30 °C; and the accelerated aging test should be performed at 44 °C for 24 hours by the traditional method or 41 °C for 24 hours using saturated NaCl solution.

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