scholarly journals Electrical conductivity for seed vigor test in sorghum (Sorghum bicolor)

2017 ◽  
Vol 1 (1) ◽  
pp. 6-12 ◽  
Author(s):  
KHODRATIEN FATONAH ◽  
IRFAN SULIANSYAH ◽  
NALWIDA ROZEN
Keyword(s):  
Electrical Conductivity ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Test Method ◽  
Water Volume ◽  
Conductivity Method ◽  
Randomized Design ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Potassium Leakage

Fatonah K, Suliansyah I, Rozen N. 2017. Electrical conductivity for seed vigor test in sorghum (Sorghum bicolor). Cell Biol Dev 1: 6-12. The objectives of this study were to obtain electrical conductivity test method for seed vigor test in sorghum, to recognize relationship between electrical conductivity test and potassium leakage, and to recognize relationship between electrical conductivity test and other variable on seed vigor. This study have two step experiments. The objective of Experiment I was to determine accurate combinations of water volume and amount seed of the electrical conductivity test for seed vigor test in sorghum. Completely Randomized Design of 15 seed lots with 3 replications were used to determine electrical conductivity method with different vigor. Experiment II were to test electrical conductivity method of 21 seed lots of sorghum without accelerated aging test and 21 seed lots of sorghum were given accelerated aging test. Completely Randomized Design of 21 seed lots with 3 replications were used in Experiment II. The variable were observed : standard germination, field emergence, speed of germination, first count, conductivity, potassium leakage and eight combination conductivity method of water volume (50, 100, 150 and 200 ml) and amount of seed sorghum (50 and 75 seed count). The result of this experiments showed that electrical conductivity test method with 150 ml water volume and 75 seed count was accurate and suitable for sorghum seed vigor test; electrical conductivity test showed positive correlation with potassium leakage;and electrical conductivity test can be used for seed vigor test in sorghum and provided the potential of physiological seed were shown through : standard germination test, field emergence test, first count test and speed of germination with negative correlation.

scholarly journals Tests for evaluating the physiological quality of pitaya seeds

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4047
Author(s):  
Thiago Alberto Ortiz ◽  
Aline Moritz ◽  
Lúcia Sadayo Assari Takahashi ◽  
Mariana Ragassi Urbano
Keyword(s):  
Electrical Conductivity ◽  
Pearson Correlation ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Water Volume ◽  
Germination Test ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

scholarly journals Tests for evaluating the physiological quality of pitaya seeds

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4047
Author(s):  
Thiago Alberto Ortiz ◽  
Aline Moritz ◽  
Lúcia Sadayo Assari Takahashi ◽  
Mariana Ragassi Urbano
Keyword(s):  
Electrical Conductivity ◽  
Pearson Correlation ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Water Volume ◽  
Germination Test ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

<p>Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe <em>Hylocereus undatus </em>fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p &lt; 0.05). The germination test for <em>H. undatus </em>seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.</p>

scholarly journals Electrical conductivity and accelerated aging in amaranth (Amaranthus crueentus L.) seeds

2018 ◽  
Vol 40 (1) ◽  
pp. 44-51
Author(s):  
Thaís D’Avila Rosa ◽  
Ariele Paula Nadal ◽  
Henrique Roberto Maldaner ◽  
Vanessa Nogueira Soares ◽  
Gizele Ingrid Gadotti ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Accelerated Aging ◽  
Water Volume ◽  
Amaranthus Cruentus ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Immersion Period ◽  
Amaranth Seed

Abstract: The objective of this study was to determine adequate conditions of water volume, seed quantity and immersion period to perform the electrical conductivity test, and to analyze the parameters of the accelerated aging test and its correlation with the the physiological potential of amaranth seeds. Five lots of amaranth seeds (Amaranthus cruentus), cultivar BRS Alegria, were used. The initial evaluation of the quality of these lots comprehended the following tests: water content, germination, first germination count, accelerated aging (traditional method, and modified versions, using unsaturated and saturated saline solution for the periods of 24, 48 and 72 h), electrical conductivity in different periods (2, 4, 6, 8 and 24 h), different quantities of water (25, 50 and 75 mL), different seed quantities (100 and 150), and field emergence. It could be concluded that the accelerated aging test at 41 °C, for 72 h, using unsaturated NaCl solution was efficient in the classification of amaranth seed lots in levels of vigor. The electrical conductivity test performed for 8 hours with 25 mL of water and 100 seeds was efficient in the classification of amaranth seeds as to their physiological potential.

scholarly journals Presence of glyphosate can harm the germination of bean seeds treated with biostimulant

2020 ◽  
Vol 36 (1) ◽  
Author(s):  
Jorgiani de Ávila ◽  
Fernanda Brito Cardoso ◽  
Sebastião Ferreira de Lima ◽  
Gustavo Ribeiro Barzotto ◽  
Mayara Santana Zanella
Keyword(s):  
Electrical Conductivity ◽  
Accelerated Aging ◽  
Cold Test ◽  
Seed Vigor ◽  
Common Beans ◽  
Accelerated Aging Test ◽  
Root And Shoot Length ◽  
Aging Test ◽  
Early Seedling ◽  
Different Doses

It is possible to cultivate common beans for a third harvest in one agricultural year due to varietal characteristics. For calendar adequacy, performing desiccation and planting often occur almost simultaneously. Germination performance of many plant species can improve with biostimulant use on seeds, however the interaction with herbicide residual molecules is unknown. The hypothesis is that seeds treated with a biostimulant in soil with glyphosate residues can eliminate the advantage of the biostimulant or increase the damage caused by the herbicide. The aim of this study was to evaluate the effect of different glyphosate reduce doses and the interaction with biostimulant on bean seed germination and vigor. The experiment was conducted using a completely randomized 2 x 5 factorial block designing factorial 2 x 5, corresponding to the presence and absence of biostimulant and five different doses of glyphosate reduce rates, with four repetitions. The conducted evaluations were first count germination, germination test, accelerated aging, cold test, root and shoot length, root and shoot dry matter and electrical conductivity. It can be concluded that the biostimulant treatment on bean seeds increased germination, seed vigor and early seedling growth, but glyphosate presence reduced those advantages, increasing electrical conductivity. However, the herbicide presence provided higher germination on the accelerated aging test.  

Reduction of the Forage Seeds Physiological Quality by the Contact with Fertilizer in the Crop-Livestock Integration

2019 ◽  
pp. 1-11
Author(s):  
Anne Caroline Dallabrida Avelino ◽  
Dayana Aparecida De Faria ◽  
Carlos Eduardo Avelino Cabral ◽  
Joadil Gonçalves De Abreu ◽  
Elisangela Clarete Camili ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Contact Time ◽  
Accelerated Aging ◽  
Germination Percentage ◽  
Randomized Design ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality ◽  
Completely Randomized Design ◽  
Seeds Germination

The aim of this study was to evaluate the effects of Brachiaria seed contact time with 05-25-15 fertilizer (N-P2O5-K2O) on the seed’s physiological quality. The experimental design was a completely randomized design with four replicates. Treatments were arranged in a 5x4 factorial, being: five seeds contact times with fertilizer (0, 24, 48, 72 and 96 hours); four forages: Marandu palisadegrass, Piatã palisadegrass, Xaraés palisadegrass and Ruziziensis grass. The performed tests were: water content, germination (first count and germination percentage), tetrazolium (viability), electrical conductivity, accelerated aging, sand emergency and emergence speed index. Contact time of 05-25-15 fertilizer with Brachiaria seeds reduces linearly: Germination, emergence and emergence speed index of Brachiaria seeds; Piatã and Xaraés palisadegrass seeds viability; vigor (electrical conductivity), except in Ruziziensis grass seeds; and seeds germination of Piatã palisadegrass submitted to accelerated aging test (vigor). Piatã palisadegrass is the least recommended, among the analyzed grasses, for intercropping in simultaneous sowing.

scholarly journals Seed vigor of Piptadenia moniliformis Benth. subjected to thermal stress

2020 ◽  
Vol 42 ◽  
pp. e51287
Author(s):  
Joyce Naiara da Silva ◽  
Monalisa Alves Diniz da Silva
Keyword(s):  
Electrical Conductivity ◽  
Sulfuric Acid ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Saturated Solution ◽  
Aging Period ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality ◽  
Concentrated Sulfuric Acid

 The objective of this work is to evaluate the influence of accelerated aging test at 41ºC on the germination and seed vigor of Piptadenia moniliformis Benth. Two experiments were conducted. Experiment 1: seeds were submitted to dormancy overcoming treatments. They were immersed into sulfuric acid for 0 (intact seeds), 5, 10, 15, 20 and 25 minutes. Experiment 2: two methods were evaluated for the accelerated aging test at 41ºC, that is, the traditional method and the saturated NaCl solution method with exposure periods of 0, 24, 48, 72 and 96 hours. After each treatment, the seeds were subjected to electrical conductivity and germination tests. Seeds overcame their dormancy when immersed into concentrated sulfuric acid for 5, 10, 15, 20 or 25 minutes, but the germination was faster when immersed for 20 minutes. The different methods for the accelerated aging test, decreased the germination potential and seed vigor of P. moniliformis after 24 hours of exposure. Regarding the method used, the saturated solution provided, in general, the best results since the seed water content was lower, thus reducing the proliferation of fungi. The electrical conductivity test showed that, by increasing the aging period regardless of the method, there is an increase in seed deterioration, corroborating with germination results, which showed that the correlation was moderate and negative and indicated that the higher the conductivity, the lower the percentage and the germination speed index. The immersion of seeds of P. moniliformis into concentrated sulfuric acid for 20 minutes provides a fast and increased germination. The methods for accelerated aging at 41ºC, traditional and saturated solution, can be used as vigor tests to evaluate the physiological quality of seeds.

scholarly journals Physiological quality of popcorn seeds assessed by the accelerated aging test

2018 ◽  
Vol 40 (4) ◽  
pp. 428-434 ◽  
Author(s):  
Cristiane da Silva Rocha ◽  
Hugo César Rodrigues Moreira Catão ◽  
Franciele Caixeta ◽  
Heloisa Karoline Kavan ◽  
Talis Melo Claudino ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Moisture Content ◽  
Accelerated Aging ◽  
Seed Moisture Content ◽  
Randomized Design ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality ◽  
Consistent Information

Abstract: Despite the insufficient internal supply of popcorn kernel cultivars, the Brazilian production is continuously increasing. Therefore, searching for tests that offer fast results and reliable information on the physiological potential of the seeds is very important. The objective of this study was to assess the efficiency of the accelerated aging test, by using different times and exposure temperatures, for the evaluation of the physiological quality of popcorn seed lots. Popcorn seeds from three lots of the hybrid AP 8203 were used. For the initial characterization of the lots, the following tests were conducted: seed moisture content, first and final germination count, and field emergence. The accelerated aging test was performed in a 3x4x2 factorial scheme. After aging, the seeds were tested for moisture content, germination, and electrical conductivity. The experiment was carried out in a completely randomized design. The characteristic vigor was affected during aging, as evidenced by the increase in the amount of leachate detected by the electrical conductivity test. Seed lot 3 was most vigorous in the aging test conducted for 48 h at 45 °C. The accelerated aging test at 42 ºC for 48 h provides consistent information to differentiate seed lots of popcorn.

scholarly journals Viability of seedling image analysis (Vigor-S) to determine the physiological potential of melon seeds

2020 ◽  
Vol 42 ◽  
Author(s):  
Moadir de Sousa Leite ◽  
Salvador Barros Torres ◽  
Francisco Guilhien Gomes Junior ◽  
Carlos Henrique Queiroz Rego ◽  
Emanoela Pereira de Paiva ◽  
...  
Keyword(s):  
Image Analysis ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Nacl Solution ◽  
Randomized Design ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Analysis System ◽  
Melon Seeds

Abstract: Melon is a vegetable crop of food and nutritional importance whose cultivation is growing worldwide. In this sense, investments in technologies are necessary, especially regarding the quality of seeds used in the establishment of new areas. This study aimed to verify the effectiveness of the Automated Seed Vigor Analysis System (Vigor-S) in the evaluation of the physiological potential of melon seeds. Eight seed lots (four lots of the hybrid Supreme and four of the hybrid Premier) were used. The physiological potential of the seeds was evaluated through tests indicated for analyzing the quality of melon seeds associated with the image analysis by using the software Vigor-S, which included the analysis of seedlings from the germination test and accelerated aging in saturated NaCl solution. The experiment was conducted in a completely randomized design, with five replications. The results indicated the effectiveness of Vigor-S, especially when it was used associated with the accelerated aging test in saturated NaCl solution, with results similar to those obtained in traditional vigor tests. Thus, the Automated Seed Vigor Analysis System (Vigor-S) was effective in evaluating the physiological potential of melon seeds.

scholarly journals Accelerated aging as vigor test for sunn hemp seeds

2017 ◽  
Vol 47 (1) ◽  
Author(s):  
Clíssia Barboza da Silva ◽  
Rafael Marani Barbosa ◽  
Roberval Daiton Vieira
Keyword(s):  
Sodium Chloride ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Saturated Solution ◽  
Crotalaria Juncea ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Sunn Hemp ◽  
Germination Vigor

ABSTRACT: This study aimed to determine the most appropriate method to assess the sunn hemp ( Crotalaria juncea L.) seed vigor in the accelerated aging test. Five seed lots from harvest 2007/2008 were evaluated for germination, vigor and seedling emergence in the field. Accelerated aging test was performed at 41°C during 48, 72 and 96 hours, with and without sodium chloride saturated solution. Then, the promising procedure was also performed for 2008/2009 and 2009/2010 harvests. In the traditional accelerated aging test, the no-uniform water acquisition by the seeds adversely affected results. Accelerated aging test with sodium chloride saturated solution is the most appropriate method to evaluate the physiological potential of sunn hemp seeds, and the combination of 41°C and 96 hours is efficient for separating of seed lots in different vigor levels.

scholarly journals Envelhecimento acelerado de sementes de Celosia argentea e Celosia cristata

2018 ◽  
Vol 24 (4) ◽  
pp. 459-465
Author(s):  
Janine Farias Menegaes ◽  
Geovana Facco Barbieri ◽  
Rogério Antônio Bellé ◽  
Ubirajara Russi Nunes ◽  
Tiéle Stucker Fernandes ◽  
...  
Keyword(s):  
Heat Exposure ◽  
Accelerated Aging ◽  
Average Moisture Content ◽  
Randomized Design ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Celosia Argentea ◽  
Celosia Cristata ◽  
Two Temperatures ◽  
Cold Chamber

Celosia argentea L. and Celosia cristata L. are plants that are differentiated by the intensity and color of their flowering, and can be used as garden or cut-flower, with characteristic propagation by seeds produced in large quantities. The present study aimed to evaluate the physiological and sanitary quality of Celosia argentea and Celosia cristata seeds subjected to the accelerated aging test. The experiment was carried out in October 2016, using a completely randomized design, in a 2 × 6 factorial scheme (two temperatures: 41 and 45 oC and six periods of heat exposure: 0 (control), 24, 48, 72, 96 and 120 h), with four replicates of 50 seeds, for the two species of cockscomb (C. argentea and C. cristata). The seed lots of the two species of celosia are from the crops in the year 2012 and were stored in a cold chamber (15 oC and 40% RH), with an average moisture content of 11% and a mean germination of 98%. After the accelerated aging test, the physiological and sanitary qualities of the seeds of the two species of celosia were evaluated. We conclude that the accelerated aging proved to be effective for the evaluation of vigor, germination and emergence in the species field, when submitted to temperatures of 41 and 45 oC up to 120 h.

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