Accelerated aging test for forage pea seeds

Forage pea is an important annual, cold climate crop with high levels of essential amino acids in its seeds, which are lacking in the composition of cereal grains. Therefore, livestock farmers use its seeds as a nutritious source of forage. To plant fields of this forage, it is necessary to know the quality of the seeds, determined through germination and vigor tests. In the literature, studies on vigor tests for forage pea seeds are scarce. This study aimed to verify the efficiency of vigor tests, with emphasis on accelerated aging to evaluate the physiological quality of forage pea seeds in order to select lots for seedling emergence in the field. Using accelerated aging tests, 10 lots of forage pea were evaluated for moisture content, thousand-seed weight, emergence percentage, emergence first count, speed index of seedlings in the field, germination, germination first count, and electrical conductivity. The following variations of methodology were used: temperatures of 41 and 42 °C, exposure times of 48 and 72 h, and different relative humidity values in the aging atmosphere, using distilled water (100 % RH), saturated solution of potassium chloride (87 % RH), and sodium chloride (76 % RH). The tests of germination, emergence first count of seedlings in the field, emergence speed index, and electrical conductivity are promising to evaluate the vigor of forage pea seed lots. The accelerated aging test conducted in a solution saturated with potassium chloride at 42 °C for 48 h is adequate to estimate the vigor of forage pea seed lots, providing information similar to that of the emergence of seedlings in the field.

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Vigor testings for evaluating the physiological quality of chia seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n6supl2p2891 ◽

2019 ◽

Vol 40 (6Supl2) ◽

pp. 2891

Author(s):

Fernanda Brito Cardoso ◽

Carlos Henrique Queiroz Rego ◽

Izabela Cristina de Oliveira ◽

Alan Mario Zuffo ◽

Ana Carina da Silva Cândido ◽

...

Keyword(s):

Electrical Conductivity ◽

Traditional Method ◽

Seedling Emergence ◽

Accelerated Aging ◽

Distilled Water ◽

Nacl Solution ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Chia (Salvia hispanica L.) is a plant native to central and western Mexico and northern Guatemala belonging to the Lamiaceae family, well known for its seeds containing antioxidants, dietary fibers, and ?-linolenic acid, which helps to prevent different diseases, such as diabetes, obesity, cancer and Alzheimer. In Brazil, there is not much information about the crop, mainly about the physiological quality of its seeds. Therefore, the objective was to establish vigor testing methods to evaluate the physiological quality of chia seeds. Five seed lots were used, in which germination tests, seedling emergence, emergence velocity index and variations in the electrical conductivity test were carried out. The volume of distilled water was 25 and 50 mL, the number of seeds was 50 and 100, the temperature was 20, 25 and 30 °C, the soaking period was 1, 2, 4, 6, 12, 18 and 24 hours and variations in there was accelerated aging test by the traditional method and saturated NaCl solution, being periods of aging as 24, 48, 72 and 96 hours and temperatures of 38, 41 and 44 °C. It is concluded that both the electrical conductivity test and the accelerated aging test are efficient in evaluating the physiological quality of chia seeds. The electrical conductivity test must be performed with 100 seeds in 50 mL of distilled water, for four hours soaking at 25 or 30 °C; and the accelerated aging test should be performed at 44 °C for 24 hours by the traditional method or 41 °C for 24 hours using saturated NaCl solution.

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Electrical conductivity and accelerated aging in amaranth (Amaranthus crueentus L.) seeds

Journal of Seed Science ◽

10.1590/2317-1545v40n1183116 ◽

2018 ◽

Vol 40 (1) ◽

pp. 44-51

Author(s):

Thaís D’Avila Rosa ◽

Ariele Paula Nadal ◽

Henrique Roberto Maldaner ◽

Vanessa Nogueira Soares ◽

Gizele Ingrid Gadotti ◽

...

Keyword(s):

Electrical Conductivity ◽

Accelerated Aging ◽

Water Volume ◽

Amaranthus Cruentus ◽

Accelerated Aging Test ◽

Aging Test ◽

Immersion Period ◽

Amaranth Seed

Abstract: The objective of this study was to determine adequate conditions of water volume, seed quantity and immersion period to perform the electrical conductivity test, and to analyze the parameters of the accelerated aging test and its correlation with the the physiological potential of amaranth seeds. Five lots of amaranth seeds (Amaranthus cruentus), cultivar BRS Alegria, were used. The initial evaluation of the quality of these lots comprehended the following tests: water content, germination, first germination count, accelerated aging (traditional method, and modified versions, using unsaturated and saturated saline solution for the periods of 24, 48 and 72 h), electrical conductivity in different periods (2, 4, 6, 8 and 24 h), different quantities of water (25, 50 and 75 mL), different seed quantities (100 and 150), and field emergence. It could be concluded that the accelerated aging test at 41 °C, for 72 h, using unsaturated NaCl solution was efficient in the classification of amaranth seed lots in levels of vigor. The electrical conductivity test performed for 8 hours with 25 mL of water and 100 seeds was efficient in the classification of amaranth seeds as to their physiological potential.

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Assessment of physiological potential of stored pea (Pisum sativum L.) seeds

Journal of Seed Science ◽

10.1590/s2317-15372013000100006 ◽

2013 ◽

Vol 35 (1) ◽

pp. 42-50 ◽

Cited By ~ 1

Author(s):

Fábio Mielezrski ◽

Julio Marcos-Filho

Keyword(s):

Electrical Conductivity ◽

Growth Habit ◽

Seedling Emergence ◽

Accelerated Aging ◽

Seed Storage ◽

Seed Vigor ◽

Seedling Length ◽

Pea Seeds ◽

Cold Chamber ◽

Pea Seed

Research on pea seed storage and the identification of efficient seed vigor tests for this species is still insufficient, especially for cultivars that produce wrinkled seeds used for consumption in natura. The objective of this study was to verify the accuracy of tests to assess the physiological potential of pea seed lots stored in different environments. Four seed lots of two cultivars, 'Telefone Alta' (indeterminate growth habit) and 'Itapuã' (determinate growth habit) were stored under different environmental conditions for 8 months: a) laboratory uncontrolled b) dry and cold chamber (10 ºC and 30% RH), c) controlled environment (20 ºC and 70% RH). Seed vigor (accelerated aging, electrical conductivity, seedling length and seedling emergence) was evaluated initially and after four and eight months storage. Results showed that the use of seed lots of high initial physiological potential is fundamental for guaranteeing the desired performance of pea seeds during storage. Wrinkled pea seeds should be stored between harvest and sowing in an environment in which the sum of the relative humidity (%) and temperature (°C) does not exceed 70. Physiological potential of pea seeds should be evaluated by at least two vigor tests; consideration to accelerated aging and electrical conductivity tests is recommended.

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Tests for evaluating the physiological quality of pitaya seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n6sup2p4047 ◽

2015 ◽

Vol 36 (6Supl2) ◽

pp. 4047

Author(s):

Thiago Alberto Ortiz ◽

Aline Moritz ◽

Lúcia Sadayo Assari Takahashi ◽

Mariana Ragassi Urbano

Keyword(s):

Electrical Conductivity ◽

Pearson Correlation ◽

Accelerated Aging ◽

Seed Vigor ◽

Water Volume ◽

Germination Test ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

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Physiological quality of popcorn seeds assessed by the accelerated aging test

Journal of Seed Science ◽

10.1590/2317-1545v40n4191101 ◽

2018 ◽

Vol 40 (4) ◽

pp. 428-434 ◽

Cited By ~ 2

Author(s):

Cristiane da Silva Rocha ◽

Hugo César Rodrigues Moreira Catão ◽

Franciele Caixeta ◽

Heloisa Karoline Kavan ◽

Talis Melo Claudino ◽

...

Keyword(s):

Electrical Conductivity ◽

Moisture Content ◽

Accelerated Aging ◽

Seed Moisture Content ◽

Randomized Design ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality ◽

Consistent Information

Abstract: Despite the insufficient internal supply of popcorn kernel cultivars, the Brazilian production is continuously increasing. Therefore, searching for tests that offer fast results and reliable information on the physiological potential of the seeds is very important. The objective of this study was to assess the efficiency of the accelerated aging test, by using different times and exposure temperatures, for the evaluation of the physiological quality of popcorn seed lots. Popcorn seeds from three lots of the hybrid AP 8203 were used. For the initial characterization of the lots, the following tests were conducted: seed moisture content, first and final germination count, and field emergence. The accelerated aging test was performed in a 3x4x2 factorial scheme. After aging, the seeds were tested for moisture content, germination, and electrical conductivity. The experiment was carried out in a completely randomized design. The characteristic vigor was affected during aging, as evidenced by the increase in the amount of leachate detected by the electrical conductivity test. Seed lot 3 was most vigorous in the aging test conducted for 48 h at 45 °C. The accelerated aging test at 42 ºC for 48 h provides consistent information to differentiate seed lots of popcorn.

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Physiological quality of soybean seeds of cultivars submitted to harvesting delay and its association with seedling emergence in the field

Journal of Seed Science ◽

10.1590/s2317-15372013000200002 ◽

2013 ◽

Vol 35 (2) ◽

pp. 147-152 ◽

Cited By ~ 12

Author(s):

Fábio Oliveira Diniz ◽

Múcio Silva Reis ◽

Luiz Antônio dos Santos Dias ◽

Eduardo Fontes Araújo ◽

Tuneo Sediyama ◽

...

Keyword(s):

Seed Quality ◽

Seedling Emergence ◽

Accelerated Aging ◽

Soybean Seeds ◽

Test Results ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality ◽

The Relationship

With the aim of studying the relationship between the physiological quality of soybean seeds submitted to three harvest times and seedling emergence in the field, seeds of cultivars UFV-16, Splendor, Vencedora, Confiança, UFV-18, UFV-TN 105, Garantia and Celeste were harvested at stages R8, R8+15 and R8+30 days. The following tests were performed: accelerated aging, electrical conductivity, emergence in sand, emergence speed index and seedling emergence in the field. Germination and vigor were reduced in seeds harvested at 30 days after stage R8 in all the tests, except for the accelerated aging test, which showed a decline in vigor seeds harvested with a delay of 15 days. Seeds of cultivars UFV-16, Confiança and Garantia showed lower physiological quality in most tests, while the seeds of cultivar Celeste had higher physiological quality than the others. There was similarity between the test results of seed quality and seedling emergence in the field.

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Tests for evaluating the physiological quality of pitaya seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n6supl2p4047 ◽

2015 ◽

Vol 36 (6Supl2) ◽

pp. 4047

Author(s):

Thiago Alberto Ortiz ◽

Aline Moritz ◽

Lúcia Sadayo Assari Takahashi ◽

Mariana Ragassi Urbano

Keyword(s):

Electrical Conductivity ◽

Pearson Correlation ◽

Accelerated Aging ◽

Seed Vigor ◽

Water Volume ◽

Germination Test ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

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Accelerated aging of melon seeds

Scientia Agricola ◽

10.1590/s0103-90162003000100012 ◽

2003 ◽

Vol 60 (1) ◽

pp. 77-82 ◽

Cited By ~ 18

Author(s):

Salvador Barros Torres ◽

Julio Marcos Filho

Keyword(s):

Salt Solution ◽

Seedling Emergence ◽

Accelerated Aging ◽

Seed Vigor ◽

Traditional Procedure ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality ◽

Melon Seeds

Accelerated aging is one of the most useful tests used for the evaluation of seed vigor but it is seldomly used to test melon (Cucumis melo L.) seeds. The objective this research was to compare different procedures of the accelerated aging test to evaluate the physiological quality of melon seeds and the efficiency of using saturated salt solution for the control of water uptake by seeds. Five seed lots each of the hybrids AF-646 and AF-682 were tested for germination, seedling emergence, traditional accelerated aging (periods 0f 48, 72 and 96 hours, at 38 or 41°C) and salt saturated accelerated aging. The accelerated aging test (traditional procedure and with salt solution) for 72h and 96h, at 38 or 41°C was sensitive to detect differences in the physiological quality of the seeds. It was also observed that the seed water content after salt saturated accelerated aging was lower and more uniform, thus presenting advantagens in relation to the traditional procedure.

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Methodological adjustment of the accelerated aging test to evaluate the vigor of chia seeds

Journal of Seed Science ◽

10.1590/2317-1545v40n2188348 ◽

2018 ◽

Vol 40 (2) ◽

pp. 173-178 ◽

Cited By ~ 1

Author(s):

Aline Klug Radke ◽

Fernanda da Motta Xavier ◽

Paulo Eduardo Rocha Eberhardt ◽

Francisco Amaral Villela ◽

Géri Eduardo Meneghello

Keyword(s):

Saline Solution ◽

Standardized Test ◽

Seedling Emergence ◽

Accelerated Aging ◽

Accelerated Aging Test ◽

Chia Seed ◽

Aging Test ◽

Conventional Test

Abstract: Chia cultivation is expanding in Brazil, but there is still no standardized test to evaluate the quality of the seeds. Therefore, vigor tests that expose seeds to stress conditions must be standardized. This study aimed to establish the methodology for the accelerated aging test in chia seeds. Six chia seed lots with similar germination behavior were used. They were submitted to germination, first germination count, seedling emergence, emergence speed index and accelerated aging tests. Aging periods of 24, 48, and 72 hours were used in the conventional methodology, and also with the use of unsaturated and saturated sodium chloride (NaCl) solutions. The results obtained from the accelerated aging tests revealed tendencies for lot separation similar to the classification of chia seed lots in relation to the physiological potential obtained in the preliminary quality tests. The accelerated aging test can be used to evaluate the physiological potential of chia seeds by using water or unsaturated saline solution. In the conventional test (water), the seeds should be exposed for 24 hours. When using unsaturated saline solution, the exposure time must be of 48 hours.

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STRESS CONDITIONS AND ITS EFFECTS ON THE PHYSIOLOGICAL POTENTIAL OF PAPAVER SP. SEEDS

Revista Científica Rural ◽

10.30945/rcr-v21i3.3087 ◽

2019 ◽

Vol 21 (3) ◽

pp. 166-182

Author(s):

Andréa Bicca Noguez Martins ◽

Ivan Ricardo Carvalho ◽

Giordano Gelain Conte ◽

Anna dos Santos Suñe ◽

Bruna Barreto Dos Reis ◽

...

Keyword(s):

Saline Solution ◽

Seedling Emergence ◽

Accelerated Aging ◽

Accelerated Aging Test ◽

Aging Test ◽

Wide Range ◽

Poppy Seeds ◽

Physiological Quality ◽

Rate Of Emergence

The physiological quality of seeds is commonly characterized by the germination and vigor, which can be defined as the sum of attributes that confers the potential for seeds to germinate, rapidly emerge and result in normal seedlings under a wide range of environmental conditions. The purpose of the study was to test alternative methodologies for the accelerated aging test to evaluate the physiological potential of poppy seeds. Samples obtained from four batches were subjected to germination test at a temperature of 20 °C, traditional accelerated aging and accelerated aging diluted with saline solution (11 g of NaCl in 100 mL-1 of water) using three temperatures, 41 °C, 42 ºC and 43 ºC , for periods of 12 and 24 hours. The percentage of germination, the rate of emergence velocity, performed together with the emergence speed and the percentage of seedling emergence test were evaluated. It is concluded that the methodology of traditional accelerated aging with temperature of 43 °C and period of 12h shows to be promising for the verification of the physiological quality of poppy seeds.

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