A rapid latex agglutination test for the detection of anti-cysticercus antibodies in cerebrospinal fluid (CSF)

Simple and rapid latex-based diagnostic tests have been used for detecting specific antigens or antibodies in several diseases. In this article, we present the preliminary results obtained with a latex agglutination test (LAT) for diagnosing neurocysticercosis by detection of antibodies in CSF. A total of 43 CSF samples were assayed by the LAT: 19 CSF samples from patients with neurocysticercosis and 24 CSF samples from patients with other neurologic disorders (neurosyphilis, n = 8; neurotoxoplasmosis, n = 3; viral meningitis, n = 4, chronic headache, n = 9). The LAT exhibited 89.5% sensitivity and 75% specificity. The use of LAT seems to be an additional approach for the screening of neurocysticercosis with advantage of simplicity and rapidity. Further studies could be performed using purified antigens and serum samples.

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Syphilis Fast Latex Agglutination Test, a Rapid Confirmatory Test

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.8.4.841-842.2001 ◽

2001 ◽

Vol 8 (4) ◽

pp. 841-842 ◽

Cited By ~ 11

Author(s):

Martha B. Fears ◽

Victoria Pope

Keyword(s):

Point Of Care ◽

Transmitted Disease ◽

Treponema Pallidum ◽

Latex Agglutination ◽

Agglutination Test ◽

Latex Agglutination Test ◽

Confirmatory Test ◽

Serum Samples ◽

Sexually Transmitted ◽

Particle Agglutination

ABSTRACT Using 255 serum samples with various reactivities, we evaluated the Syphilis Fast latex agglutination test (Syphilis Fast) against theTreponema pallidum particle agglutination test (TP-PA) for confirming a diagnosis of syphilis. We found 98.8% agreement between the Syphilis Fast and the TP-PA. The Syphilis Fast, however, had a couple of advantages over the TP-PA: the test takes only 8 min to perform and produces results that are easy to read. It appears to be a good confirmatory test for syphilis, especially for point-of-care clinics such as prenatal or sexually transmitted disease clinics.

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Effects of Pretreating Serum Samples on the Performance of a Latex Agglutination Test for Serodiagnosis of Paracoccidioidomycosis

Clinical and Vaccine Immunology ◽

10.1128/cvi.05274-11 ◽

2011 ◽

Vol 19 (3) ◽

pp. 386-390 ◽

Cited By ~ 5

Author(s):

Fabíola Silveira-Gomes ◽

Silvia Helena Marques-da-Silva

Keyword(s):

Sensitivity And Specificity ◽

Bacterial Infections ◽

Paracoccidioides Brasiliensis ◽

Latex Agglutination ◽

Agglutination Test ◽

Latex Agglutination Test ◽

Serum Samples ◽

Content Type ◽

Human Sera ◽

Normal Human

ABSTRACTParacoccidioidomycosis (PCM) is a fungal disease caused byParacoccidioides brasiliensis, and Brazil is one of the principal countries where it is endemic. Diagnosis is based on the observation of buddingP. brasiliensisyeast in clinical specimens from patients; however, the sensitivity of the visualization of fungi is low, indicating that serological tests are used for early diagnosis. The double-immunodiffusion test (ID) is the “gold standard” test for serology in PCM, although the execution of this test requires the availability of laboratorial infrastructure. We report the improved performance of a latex agglutination test (LAT) by pretreating 30 serum samples from PCM patients and 71 controls (histoplasmosis and aspergillosis patients, patients with bacterial infections, and normal human sera) with a dilution buffer incubated at 37°C for 30 min. The sensitivity and specificity of the LAT test in the nonpretreated samples were 73% and 79%, respectively. However, when samples were pretreated, the sensitivity and specificity of the test increased to 90%. In this study, we did not observe cross-reactivity with histoplasmosis patient sera, but some reactions to sera from patients with aspergillosis and bacterial infections were noted. Normal human sera were not reactive in our tests. These results indicate the need for the elimination of heterologous reactions so that we can adequately use this method for screening cases of PCM.

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Diagnostic Study of Toxoplasmosis in Domestic Chickens in Sulaimani Province

Al-Qadisiyah Journal of Veterinary Medicine Sciences ◽

10.29079/vol12iss2art259 ◽

2013 ◽

Vol 12 (2) ◽

pp. 63 ◽

Cited By ~ 3

Author(s):

A. A. Mohammed

Keyword(s):

Toxoplasma Gondii ◽

Histopathological Examination ◽

Latex Agglutination ◽

Agglutination Test ◽

Gallus Domesticus ◽

Latex Agglutination Test ◽

Diagnostic Study ◽

Serum Samples ◽

First Report ◽

Seropositive Rate

Toxoplasma gondii causes toxoplasmosis in human and animals, a disease of cosmopolitan character. A total of 65 serum samples of domestic chickens (Gallus domesticus) from Sulaimani region were collected from May till August 2012.The overall seropositivity against T. gondii antibody was 60% based on the Latex agglutination test (LAT). The positive agglutination titers were ranged between 1:2 -1:128, and the highest seropositive rate observed at a titer 1: 128 was 25.64% . The present study demonstrated that anti-Toxoplasma antibodies were high in chickens in this study.In histopathological examination of brain samples, tissue cysts were observed in 7 samples 33.33%. About the Giemsa stained impression tissue smears of liver, kidney and spleen of all seropositive chickens tissue cysts also observed at the rate of 38.46%, 20.51% and 12.82% respectively, the results indicate that T. gondii localized in the liver more often than in other tissues of naturally infected chickens.This is the first report of T. gondii infection in domestic chickens in Sulaimani province.

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