Detection of Trypanosoma cruzi and Trypanosoma rangeli infection in triatomine vectors by amplification of the histone H2A/SIRE and the sno-RNA-C11 genes

Trypanosoma rangeli is non pathogenic for humans but of important medical and epidemiological interest because it shares vertebrate hosts, insect vectors, reservoirs and geographic areas with T. cruzi, the etiological agent of Chagas disease. Therefore, in this work, we set up two PCR reactions, TcH2AF/R and TrFR2, to distinguish T. cruzi from T. rangeli in mixed infections of vectors based on amplification of the histone H2A/SIRE and the small nucleolar RNA Cl1 genes, respectively. Both PCRs were able to appropriately detect all T. cruzi or T. rangeli experimentally infected-triatomines, as well as the S35/S36 PCR which amplifies the variable region of minicircle kDNA of T. cruzi. In mixed infections, whereas T. cruzi DNA was amplified in 100% of samples with TcH2AF/R and S35/S36 PCRs, T. rangeli was detected in 71% with TrF/R2 and in 6% with S35/S36. In a group of Rhodnius colombiensis collected from Coyaima (Colombia), T. cruzi was identified in 100% with both PCRs and T. rangeli in 14% with TrF/R2 and 10% with S35/S36 PCR. These results show that TcH2AF/R and TrF/R2 PCRs which are capable of recognizing all T. cruzi and T. rangeli strains and lineages could be useful for diagnosis as well as for epidemiological field studies of T. cruzi and T. rangeli vector infections.

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Limit of detection of PCR/RFLP analysis of cytochrome oxidase II for the identification of genetic groups of Trypanosoma cruzi and Trypanosoma rangeli in biological material from vertebrate hosts

Parasitology Research ◽

10.1007/s00436-018-5928-1 ◽

2018 ◽

Vol 117 (8) ◽

pp. 2403-2410 ◽

Cited By ~ 2

Author(s):

Amanda Regina Nichi Sá ◽

Karen Yuki Kimoto ◽

Mário Steindel ◽

Edmundo Carlos Grisard ◽

Mônica Lúcia Gomes

Keyword(s):

Trypanosoma Cruzi ◽

Cytochrome Oxidase ◽

Biological Material ◽

Limit Of Detection ◽

Rflp Analysis ◽

Trypanosoma Rangeli ◽

Genetic Groups ◽

Pcr Rflp ◽

Vertebrate Hosts ◽

Cytochrome Oxidase Ii

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Clinical Trypanosoma rangeli infection as a complication of Chagas‘ disease

Parasitology ◽

10.1017/s0031182000055827 ◽

1987 ◽

Vol 94 (3) ◽

pp. 475-484 ◽

Cited By ~ 38

Author(s):

F. Guhl ◽

L. Hudson ◽

C. J. Marinkelle ◽

C. A. Jaramillo ◽

D. Bridge

Keyword(s):

Trypanosoma Cruzi ◽

Chagas Disease ◽

Western Blotting ◽

Mixed Infections ◽

Laboratory Studies ◽

Trypanosoma Rangeli ◽

Active Infection ◽

Binding Reaction ◽

Rio Negro ◽

Similar Range

Laboratory studies on a group of 20 patients from the Rio Negro Valley, Colombia selected for detailed study showed that 14 gave antibody reactions on immunoassay consistent with Trypanosoma cruzi or T. rangeli infections. Four were diagnosed as having T. rangeli infection, 4 had mixed infections and 6 were infected with T. cruzi alone. Immunoprecipitation analysis showed that sera from T. crwzi-infected patients recognized a similar range of trypomastigote-derived polypeptides as sera from patients in Brazil, and all of the Colombian sera reacted with the 160 kiloDalton (kDa) polypeptide associated with active infection. Although sera from patients with T. rangeli infection alone gave a positive immunofluorescence or ELISA reaction with T. rangeli, they failed to bind to parasite polypeptides by either immunoprecipitation or Western blotting. Intriguingly, sera from patients with mixed infections consistently gave a stronger, but qualitatively similar, binding reaction in immunoprecipitation and Western blotting compared to sera from patients infected with T. cruzi alone.

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Trypanosoma cruzi and Trypanosoma rangeli co-infection patterns in insect vectors vary across habitat types in a fragmented forest landscape

Parasitology Open ◽

10.1017/pao.2016.9 ◽

2016 ◽

Vol 2 ◽

Cited By ~ 6

Author(s):

NICOLE L. GOTTDENKER ◽

LUIS F. CHAVES ◽

JOSE E. CALZADA ◽

JENNIFER K. PETERSON ◽

ANAMARIA SANTAMARÍA ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

Forest Landscape ◽

Insect Vectors ◽

Trypanosome Infection ◽

Trypanosoma Rangeli ◽

Habitat Types ◽

Fragmented Forest ◽

Triatomine Bugs ◽

Disturbed Habitats ◽

Rhodnius Pallescens

SUMMARYThe transmission of parasites can be influenced by their co-occurrence with other parasites, in some cases increasing or reducing transmission.Trypanosoma cruzi, aetiologic agent of Chagas disease, often co-occurs withTrypanosoma rangeli, a parasite not pathogenic for mammal hosts. Both parasites can reduce the fitness of their insect vectors (the triatomine bugs; Hemiptera: Reduviidae), withT. rangelibeing more pathogenic for some species. Here, we study the prevalence ofT. cruziandT. rangeliin the triatomineRhodnius pallescensacross a heterogeneously transformed landscape in Panamá. We found that singleT. rangeliinfections were more common in contiguously forested habitats, while singleT. cruziinfections predominated in anthropogenically disturbed habitats.Trypanosoma cruzi–T. rangelico-infections were more common in contiguous forests and in peridomiciliary areas. Furthermore, adult insects were more likely to be co-infected than nymphs. Our results suggest that human-mediated landscape transformation might have increased the predominance of single infections withT. cruziwithin vectors. An important mechanism driving changes in trypanosome infection patterns in triatomines at a landscape scale includes alterations in host species composition that may vary with different degrees of deforestation. Trypanosome co-infection may also confer a survival advantage forR. pallescensto and/or throughout adulthood.

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