scholarly journals SAINT LOUIS ENCEPHALITIS VIRUS IN MATO GROSSO, CENTRAL-WESTERN BRAZIL

2015 ◽  
Vol 57 (3) ◽  
pp. 215-220 ◽  
Author(s):  
Letícia Borges da Silva HEINEN ◽  
Nayara ZUCHI ◽  
Otacília Pereira SERRA ◽  
Belgath Fernandes CARDOSO ◽  
Breno Herman Ferreira GONDIM ◽  
...  
Keyword(s):  
Yellow Fever Virus ◽  
Febrile Illness ◽  
The State ◽  
Encephalitis Virus ◽  
Serum Samples ◽  
Mato Grosso ◽  
Saint Louis ◽  
Reverse Transcription Pcr ◽  
The City ◽  
Saint Louis Encephalitis

The dengue virus (DENV), which is frequently involved in large epidemics, and the yellow fever virus (YFV), which is responsible for sporadic sylvatic outbreaks, are considered the most important flaviviruses circulating in Brazil. Because of that, laboratorial diagnosis of acute undifferentiated febrile illness during epidemic periods is frequently directed towards these viruses, which may eventually hinder the detection of other circulating flaviviruses, including the Saint Louis encephalitis virus (SLEV), which is widely dispersed across the Americas. The aim of this study was to conduct a molecular investigation of 11 flaviviruses using 604 serum samples obtained from patients during a large dengue fever outbreak in the state of Mato Grosso (MT) between 2011 and 2012. Simultaneously, 3,433 female Culex spp. collected with Nasci aspirators in the city of Cuiabá, MT, in 2013, and allocated to 409 pools containing 1-10 mosquitoes, were also tested by multiplex semi-nested reverse transcription PCR for the same flaviviruses. SLEV was detected in three patients co-infected with DENV-4 from the cities of Cuiabá and Várzea Grande. One of them was a triple co-infection with DENV-1. None of them mentioned recent travel or access to sylvatic/rural regions, indicating that transmission might have occurred within the metropolitan area. Regarding mosquito samples, one pool containing one Culex quinquefasciatus female was positive for SLEV, with a minimum infection rate (MIR) of 0.29 per 1000 specimens of this species. Phylogenetic analysis indicates both human and mosquito SLEV cluster, with isolates from genotype V-A obtained from animals in the Amazon region, in the state of Pará. This is the first report of SLEV molecular identification in MT.

scholarly journals Epidemiology of Saint Louis encephalitis virus in the Brazilian Amazon region and in the State of Mato Grosso do Sul, Brazil: elevated prevalence of antibodies in horses

2010 ◽  
Vol 1 (1) ◽  
Author(s):  
Sueli Guerreiro Rodrigues ◽  
Otávio Pinheiro Oliva ◽  
Francisco Anilton Alves Araujo ◽  
Lívia Carício Martins ◽  
Janiffer Oliveira Chiang ◽  
...  
Keyword(s):  
Brazilian Amazon ◽  
Amazon Region ◽  
The State ◽  
Encephalitis Virus ◽  
Mato Grosso ◽  
Saint Louis ◽  
Brazilian Amazon Region ◽  
Saint Louis Encephalitis ◽  
Mato Grosso Do Sul

scholarly journals Infection with Saint Louis encephalitis virus in the city of Ribeirao Preto, Brazil: report of one case

2014 ◽  
Vol 26 ◽  
pp. 96-97 ◽  
Author(s):  
Felipe Gonçalves Motta Maia ◽  
Juliana Helena Chávez ◽  
William Marciel de Souza ◽  
Marilia Farignoli Romeiro ◽  
Luiza Antunes de Castro-Jorge ◽  
...  
Keyword(s):  
Encephalitis Virus ◽  
Saint Louis ◽  
The City ◽  
Saint Louis Encephalitis

scholarly journals DENGUE OUTBREAK IN MATO GROSSO STATE, MIDWESTERN BRAZIL

2015 ◽  
Vol 57 (6) ◽  
pp. 489-496 ◽  
Author(s):  
Letícia Borges da Silva HEINEN ◽  
Nayara ZUCHI ◽  
Belgath Fernandes CARDOSO ◽  
Marcelo Adriano Mendes dos SANTOS ◽  
Mauricio Lacerda NOGUEIRA ◽  
...  
Keyword(s):  
Asian American ◽  
Yellow Fever Virus ◽  
Febrile Illness ◽  
Rt Pcr ◽  
Serum Samples ◽  
Mato Grosso ◽  
Denv Serotypes ◽  
Genotype Iii ◽  
Large Outbreak ◽  
Southeast Asian American

Dengue virus (DENV) is the most frequent arbovirus worldwide. In this study, we report a large outbreak in Mato Grosso State (MT). Serum samples from 604 patients with acute febrile illness for less than five days were inoculated in C6/36 cells, then infected cells were subjected to an indirect immunofluorescence test for DENV serotypes and yellow fever virus. Serum samples were submitted to a multiplex-semi-nested-RT-PCR for 11 flaviviruses. DENV-4 was isolated in 150/604 (24.8%) and DENV-1 in 19/604 (3.1%) specimens. By RT-PCR, 331 (54.8%) samples tested positive for DENV; 321 had single infections (DENV-4 n = 305; DENV-1 n = 15; DENV-3 n = 1), nine had co-infections of DENV-1/DENV-4, and one of DENV-2/DENV-4. DENV-4 was detected in 315/331 (95.2%) positive patients from 17 municipalities, and DENV-1 in 24/331 (7.2%) patients from five cities in north-central MT and the city of Cuiaba. The incidence of infection was higher in patients aged 20-39 (142/331; 42.9%). The NS5 partial nucleotide sequence of DENV-1 was most similar to that of genotype V, DENV-2 to Southeast Asian/American, DENV-3 to genotype III, and DENV-4 to genotype II strains, considered the most frequent strains in Brazil. This outbreak coincided with the introduction of DENV-4 in the state. Cuiaba was hyperendemic for the four DENV serotypes, highlighting the necessity for arbovirus surveillance in MT.

Thiosemicarbazones and Phthalyl-Thiazoles compounds exert antiviral activity against yellow fever virus and Saint Louis encephalitis virus

2017 ◽  
Vol 87 ◽  
pp. 381-387 ◽  
Author(s):  
Carolina Colombelli Pacca ◽  
Rafael Elias Marques ◽  
José Wanderlan P. Espindola ◽  
Gevânio B.O.Oliveira Filho ◽  
Ana Cristina Lima Leite ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Yellow Fever ◽  
Yellow Fever Virus ◽  
Encephalitis Virus ◽  
Fever Virus ◽  
Saint Louis ◽  
Saint Louis Encephalitis

Improvement of the purification of Saint Louis encephalitis virus NS2B-NS3 recombinant protease expressed in Escherichia coli

2008 ◽  
Vol 868 (1-2) ◽  
pp. 58-63 ◽  
Author(s):  
Boris Pastorino ◽  
Dominique Rolland ◽  
Christophe N. Peyrefitte ◽  
Nathalie Wurtz ◽  
Lionel Almeras ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Encephalitis Virus ◽  
Saint Louis ◽  
Saint Louis Encephalitis

scholarly journals Virulence variation among epidemic and non-epidemic strains of Saint Louis encephalitis virus circulating in Argentina

2014 ◽  
Vol 109 (2) ◽  
pp. 197-201 ◽  
Author(s):  
María Elisa Rivarola ◽  
Laura Beatriz Tauro ◽  
Guillermo Albrieu Llinás ◽  
Marta Silvia Contigiani
Keyword(s):  
Encephalitis Virus ◽  
Saint Louis ◽  
Saint Louis Encephalitis ◽  
Virulence Variation

scholarly journals Saint Louis Encephalitis Virus, Brazil

2007 ◽  
Vol 13 (1) ◽  
pp. 176-178 ◽  
Author(s):  
Adriano Mondini ◽  
Izabela Lídia Soares Cardeal ◽  
Eduardo Lázaro ◽  
Silva H. Nunes ◽  
Cibele C. Moreira ◽  
...  
Keyword(s):  
Encephalitis Virus ◽  
Saint Louis ◽  
Saint Louis Encephalitis

scholarly journals Seroprevalence of Toxoplasma gondii infection in swine matrices in Nova Mutum and Diamantino, Mato Grosso, Brazil

2010 ◽  
Vol 19 (4) ◽  
pp. 254-255 ◽  
Author(s):  
Lívia Saab Muraro ◽  
João Garcia Caramori Júnior ◽  
Maria Regina Reis Amendoeira ◽  
Joyce Alves Pereira ◽  
João Xavier de Oliveira Filho ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
The State ◽  
Indirect Fluorescent Antibody ◽  
Risk Of Infection ◽  
Serum Samples ◽  
Mato Grosso ◽  
Toxoplasma Gondii Infection

This report aimed to assess the seroprevalence of Toxoplasma gondii infection in 708 swine matrices in Nova Mutum and Diamantino in the state of Mato Grosso, Central-West Brazil. Serum samples were examined by indirect fluorescent antibody test (IFAT). It was found a seroprevalence of 12.8%, considering titers >64. Therefore, the data reinforce the need for appropriate management of swine raising to minimize the risk of infection of pigs with T. gondii.

Serological survey for Saint Louis encephalitis virus and West Nile virus in domestic mammals in Córdoba, Argentina: are our pets potential sentinels?

2020 ◽  
Vol 165 (9) ◽  
pp. 2079-2082
Author(s):  
Luisina Currenti ◽  
Paula Tasca ◽  
María del Pilar Díaz ◽  
Marta Contigiani ◽  
Lorena Spinsanti
Keyword(s):  
West Nile Virus ◽  
Encephalitis Virus ◽  
Serological Survey ◽  
West Nile ◽  
Saint Louis ◽  
Saint Louis Encephalitis

scholarly journals A Computational Assay to Design an Epitope-Based Peptide Vaccine against Saint Louis Encephalitis Virus

2013 ◽  
Vol 7 ◽  
pp. BBI.S13402 ◽  
Author(s):  
Anayet Hasan ◽  
Mehjabeen Hossain ◽  
Jibran Alam
Keyword(s):  
T Cell ◽  
B Cell ◽  
In Silico ◽  
Peptide Vaccine ◽  
Encephalitis Virus ◽  
Peptide Sequence ◽  
Cell Mediated Immunity ◽  
Protein Database ◽  
Saint Louis ◽  
Saint Louis Encephalitis

Saint Louis encephalitis virus, a member of the flaviviridae subgroup, is a culex mosquito-borne pathogen. Despite severe epidemic outbreaks on several occasions, not much progress has been made with regard to an epitope-based vaccine designed for Saint Louis encephalitis virus. The envelope proteins were collected from a protein database and analyzed with an in silico tool to identify the most immunogenic protein. The protein was then verified through several parameters to predict the T-cell and B-cell epitopes. Both T-cell and B-cell immunity were assessed to determine that the protein can induce humoral as well as cell-mediated immunity. The peptide sequence from 330–336 amino acids and the sequence REYCYEATL from the position 57 were found as the most potential B-cell and T-cell epitopes, respectively. Furthermore, as an RNA virus, one important thing was to establish the epitope as a conserved one; this was also done by in silico tools, showing 63.51% conservancy. The epitope was further tested for binding against the HLA molecule by computational docking techniques to verify the binding cleft epitope interaction. However, this is a preliminary study of designing an epitope-based peptide vaccine against Saint Louis encephalitis virus; the results awaits validation by in vitro and in vivo experiments.

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