scholarly journals Interleukin- 2 production during murine infection by Leishmania mexicana amazonensis

1986 ◽  
Vol 81 (1) ◽  
pp. 43-47 ◽  
Author(s):  
Manoel Barral-Netto ◽  
Silene B. Roters ◽  
Aldina Barral
Keyword(s):  
Lymph Node ◽  
Concanavalin A ◽  
Post Infection ◽  
Similar Pattern ◽  
Interleukin 2 ◽  
Leishmania Mexicana ◽  
Susceptibility To Infection ◽  
F1 Progeny ◽  
Lymph Node Cells ◽  
Foot Pad

Highly susceptible BALB/c mice, resistant C57B1/6 and their F1 progeny (BDF1) were infected subcutaneously in the foot pad with Leishmania mexicana amazonenesis. At various times after infection, spleen or draining popliteal lymph node cells were assayed for their capacity to generate Interleukin-2 (I1-2) by Concanavalin A (ConA) stimulation. In both BALB/c and C57B1/6 strains there was a transient increase in their capacity to produce I1-2, from the 3rd to the 10th week post-infection. Return to pre-infection levels ocurred between 13th to 16th week post-infection in all three strains. BALB/c mice always produced higher titers of 11-2 than C57B1/6, but such differences were statistically significant only at 3 and 10 weeks post-infection. BDF1 mice had titers similar to those observed in BALB/c mice. I1-2 production by ConA-stimulated lymph node cells was lower as compared to the spleen, but with a similar pattern among the three mice strains. Our data show that susceptibility to infection by l. mexicana amazonenesis is not associated with deficient ConA-stimulated I1-2 production.

scholarly journals Leishmania (Viannia) panamensis - induced cutaneous leishmaniasis in susceptible and resistant mouse strains

1995 ◽  
Vol 37 (6) ◽  
pp. 475-481 ◽  
Author(s):  
H. Goto ◽  
J. I. Rojas ◽  
L. Sporrong ◽  
P. de Carreira ◽  
C. Sánchez ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Post Infection ◽  
Proliferative Response ◽  
Cellular Response ◽  
Initial Response ◽  
Mouse Strains ◽  
Resistant Mouse ◽  
Inoculation Site ◽  
Lymph Node Cells ◽  
Foot Pad

We studied the susceptibility to Leishmania (Viannia) panamensis in strains of mice. The C57BL/6 strain was resistant and showed self-controlled lesion at the injected foot pad. The BALB/c and DBA/2J strains were susceptible and showed a foot swelling that started day 20 post-infection and progressed to a tumour-like lesion in later period of observation. The CBA/HJ strain was found to be of intermediary resistance. In contrast to other known cutaneous leishmaniasis in mice, the lesion in L. (V.) panamensis-infected mice was restricted to the inoculation site in the skin. In addition, we studied the development of cellular response and antibodies against Leishmania antigen in BALB/c and C57BL/6 strains. The proliferative response of lymph node cells against L. (V.) panamensis antigen was biphasic in both strains. An initial response was seen on day 20, followed by a refractory period between 40 and 80 days and a second response around fourth month post-infection. The response in the latter period was higher in C57BL/6 strain than in BALB/c strain. BALB/c strain presented much higher anti-Leishmania antibody level than C57BL/6 strain. The model and the correlation of immunological variables and the course of the infection are discussed.

scholarly journals Recombinant Galectin-1 and Its Genetic Delivery Suppress Collagen-Induced Arthritis via T Cell Apoptosis

1999 ◽  
Vol 190 (3) ◽  
pp. 385-398 ◽  
Author(s):  
Gabriel A. Rabinovich ◽  
Gordon Daly ◽  
Hanna Dreja ◽  
Hitakshi Tailor ◽  
Clelia M. Riera ◽  
...  
Keyword(s):  
Lymph Node ◽  
T Cell ◽  
Interleukin 2 ◽  
Disease Onset ◽  
Therapeutic Effects ◽  
Collagen Induced Arthritis ◽  
Gene Therapy Protocol ◽  
Lymph Node Cells

Galectin-1 (GAL-1), a member of a family of conserved β-galactoside–binding proteins, has been shown to induce in vitro apoptosis of activated T cells and immature thymocytes. We assessed the therapeutic effects and mechanisms of action of delivery of GAL-1 in a collagen-induced arthritis model. A single injection of syngeneic DBA/1 fibroblasts engineered to secrete GAL-1 at the day of disease onset was able to abrogate clinical and histopathological manifestations of arthritis. This effect was reproduced by daily administration of recombinant GAL-1. GAL-1 treatment resulted in reduction in anticollagen immunoglobulin (Ig)G levels. The cytokine profile in draining lymph node cells and the anticollagen IgG isotypes in mice sera at the end of the treatment clearly showed inhibition of the proinflammatory response and skewing towards a type 2–polarized immune reaction. Lymph node cells from mice engaged in the gene therapy protocol increased their susceptibility to antigen-induced apoptosis. Moreover, GAL-1–expressing fibroblasts and recombinant GAL-1 revealed a specific dose-dependent inhibitory effect in vitro in antigen-dependent interleukin 2 production to an Aq-restricted, collagen type 2–specific T cell hybridoma clone. Thus, a correlation between the apoptotic properties of GAL-1 in vitro and its immunomodulatory properties in vivo supports its therapeutic potential in the treatment of T helper cell type 1–mediated autoimmune disorders.

Interleukin-2 Responses of MRL/lpr Mouse Splenocytes and Lymph Node Cells Induced by TPA and A23187

1987 ◽  
Vol 83 (2) ◽  
pp. 167-173 ◽  
Author(s):  
Takuya Katagiri ◽  
Hiroko Tomiyama ◽  
Shigeru Kyuwa ◽  
Kyoichi Kano
Keyword(s):  
Lymph Node ◽  
Interleukin 2 ◽  
Mouse Splenocytes ◽  
Lymph Node Cells

scholarly journals Increased Capacity for Interleukin-2 Synthesis Parallels Disease Progression in Mice Infected withLeishmania major

1998 ◽  
Vol 66 (9) ◽  
pp. 4537-4540 ◽  
Author(s):  
Frederick P. Heinzel ◽  
Ronald M. Rerko ◽  
Andrea M. Hujer ◽  
Richard A. Maier
Keyword(s):  
Lymph Node ◽  
Disease Progression ◽  
Functional Significance ◽  
Interleukin 2 ◽  
Gamma Interferon ◽  
Mrna Levels ◽  
Lymph Node Cells

ABSTRACT Lymph node cells of BALB/c mice with progressive leishmaniasis produced sixfold more interleukin-2 (IL-2) in culture than those of healing C57BL/6 mice. IL-2 synthesis also increased in C57BL/6 mice made susceptible by IL-12 or gamma interferon deficiency. However, IL-2 mRNA levels in vivo did not reflect IL-2 production in vitro. Because IL-2 contributes to the pathogenesis of progressive leishmaniasis, the functional significance of these findings should be further explored.

scholarly journals Lymph node cell responsiveness in BALB/C mice infected with Leishmania mexicana

1985 ◽  
Vol 80 (2) ◽  
pp. 135-140
Author(s):  
Hilda A. Pérez ◽  
José Bolívar
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
Lymph Node Cell ◽  
Leishmania Mexicana ◽  
Blastogenic Response ◽  
Lymph Node Cells ◽  
Spleen And Lymph Nodes

In the present study we measured the blastogenic response of lymph node cells from BALB/c mice infected with Leishmania mexicana throughout the course of infection. Results showed that infected mice displayed normal blastogenic responses in the lymph nodes until twenty weeks of infection. Thereafter, there was a gradual suppression. Comparison of the immunoresponsiveness in the spleen and lymph nodes, revealed normal responses in the lymph nodes several weeks after suppression in the spleen had occurred. Suppression of blastogenic responses in the lymph nodes was related to an adherent macrophage-like cell which actively suppressed normal proliferative responses to mitogens.

Characterization of resting and phorbol ester or concanavalin A activated bovine lymph node cells with leukocyte specific monoclonal antibodies

1994 ◽  
Vol 40 (1) ◽  
pp. 49-61 ◽  
Author(s):  
David J. Hurley ◽  
Richard A. Wilson ◽  
Cynthia L. Baldwin ◽  
Jing-Yi Liu ◽  
Andrea M. Mastro
Keyword(s):  
Monoclonal Antibodies ◽  
Lymph Node ◽  
Phorbol Ester ◽  
Concanavalin A ◽  
Lymph Node Cells
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