Detection of Human Epididymis Protein 4 (HE4) in Human Serum Samples Using a Specific Monoclonal Antibody-Based Sandwich Enzyme-Linked Immunosorbent Assay (ELISA)

ABSTRACTAnthrax, caused byBacillus anthracis, is primarily a zoonotic disease. Being a public health problem also in several developing countries, its early diagnosis is very important in human cases. In this study, we describe the use of an indirect enzyme-linked immunosorbent assay (ELISA) for detection of anti-lethal factor (anti-LF) IgG in human serum samples. A panel of 203 human serum samples consisting of 50 samples from patients with confirmed cutaneous anthrax, 93 samples from healthy controls from areas of India where anthrax is nonendemic, 44 samples from controls from an area of India where anthrax is endemic, and 16 patients with a disease confirmed not to be anthrax were evaluated with an anti-LF ELISA. The combined mean anti-LF ELISA titer for the three control groups was 0.136 ELISA unit (EU), with a 95% confidence interval (CI) of 0.120 to 0.151 EU. The observed sensitivity and specificity of the ELISA were 100% (95% CI, 92.89 to 100%) and 97.39% (95% CI, 93.44 to 99.28%), respectively, at a cutoff value of 0.375 EU, as decided by receiver operating characteristic (ROC) curve analysis. The likelihood ratio was found to be 49.98. The positive predictive value (PPV), negative predictive value (NPV), efficiency, and Youden's index (J) for reliability of the assay were 92.5%, 100%, 98.02%, and 0.97, respectively. The false-positive predictive rate and false-negative predictive rate of the assay were 2.61% and 0%. The assay could be a very useful tool for early diagnosis of cutaneous anthrax cases, as antibodies against LF appear much earlier than those against other anthrax toxins in human serum samples.

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Evaluation of a monoclonal antibody affinity purified antigen for zymodeme specific serological diagnosis of Trypanosoma cruzi infection

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02761987000100014 ◽

1987 ◽

Vol 82 (1) ◽

pp. 81-85 ◽

Cited By ~ 1

Author(s):

Mauro Schechter

Keyword(s):

Monoclonal Antibody ◽

Trypanosoma Cruzi ◽

Affinity Chromatography ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Specific Monoclonal Antibody ◽

Antibody Affinity ◽

Specific Diagnosis ◽

Purified Antigen ◽

Cruzi Infection

Theoretically, serological assays with affinity purified marker antigens can allow strain-specific diagnosis even when parasites cannot be retrieved from and infected host. A Trypanosoma cruzi antigen was purified by affinity chromatography using a zymodeme (Z) 2 specific monoclonal antibody (2E2C11). An indirect enzyme-linked immunosorbent assay (ELISA) based on the purified antigen could discriminate between sera from rabbits immunized with T. cruzi zymodeme clones but could not discriminate between sera from mice infected with different zymodemes.

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Development of an Enzyme-Linked Immunosorbent Assay System for the Determination of Asymmetric Dimethylarginine Using a Specific Monoclonal Antibody

Bioscience Biotechnology and Biochemistry ◽

10.1271/bbb.110727 ◽

2012 ◽

Vol 76 (2) ◽

pp. 400-403 ◽

Cited By ~ 4

Author(s):

Miyuki YOKORO ◽

Makiko SUZUKI ◽

Machiko YATANI ◽

Hiromi YAMASHITA ◽

Yoshitaka TAKAHASHI ◽

...

Keyword(s):

Monoclonal Antibody ◽

Immunosorbent Assay ◽

Asymmetric Dimethylarginine ◽

Assay System ◽

Enzyme Linked Immunosorbent Assay ◽

Specific Monoclonal Antibody

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Seroprevalence of Jamestown Canyon virus in the Japanese general population

BMC Infectious Diseases ◽

10.1186/s12879-020-05517-2 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Hirofumi Kato ◽

Masaaki Satoh ◽

Madoka Kawahara ◽

Satoshi Kitaura ◽

Tomoki Yoshikawa ◽

...

Keyword(s):

General Population ◽

Human Serum ◽

Health Agency ◽

Febrile Illness ◽

Central Nervous System Disease ◽

Enzyme Linked Immunosorbent Assay ◽

Antibody Assay ◽

Serum Samples ◽

Human Serum Samples ◽

Jamestown Canyon Virus

Abstract Background Jamestown Canyon virus (JCV) is a mosquito-borne orthobunyavirus that causes acute febrile illness, meningitis, and meningoencephalitis, mainly among adults. JCV is widely distributed in North America and the number of JCV cases in the U.S. has increased in recent years. Therefore, the central nervous system disease caused by JCV can be considered a potentially re-emerging viral disease. However, the seroprevalence of JCV is unknown in Japan. The purpose of this study is to evaluate the seroprevalence of JCV in the Japanese population. Methods We used an IgG enzyme-linked immunosorbent assay (IgG-ELISA) with JCV-infected cell-lysates and/or a neutralizing (NT) antibody assay. The cut-off value of IgG-ELISA was determined using IgG-ELISA to analyze serum specimens from 37 healthy Japanese donors. IgG-ELISA was validated by assessing its sensitivity and specificity, using 38 human serum samples previously tested for the presence or absence of antibodies against JCV and snowshoe hare virus (SSHV), in an in-house NT antibody assay conducted by the Public Health Agency of Canada. The seroepidemiological study was performed using IgG-ELISA and NT antibody assay to analyze 246 human serum samples from the serum bank of the National Institute of Infectious Diseases (NIID) in Japan. Results The cut-off value of IgG-ELISA was determined at 0.20, based on the mean (− 0.075) and standard deviation (0.092) values using Japanese donors’ sera. The sensitivity and the specificity of IgG-ELISA determined using 25 JCV-positive and 4 JCV-negative serum samples were 96 and 100%, respectively. Analysis of the 246 Japanese serum samples revealed that no specimen showed a higher value than the cut-off value of IgG-ELISA, and no sample tested positive by the NT antibody assay. Conclusions Our results showed that JCV is not circulating significantly in Japan. To the best of our knowledge, this is the first report to demonstrate the seroprevalence of JCV in the general population in Japan.

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