Rumen Development of Artificially-Reared Lambs Exposed to Three Different Rearing Regimens

The objective of this study was to examine the effect of three different rearing regimens on rumen development in lambs reared artificially. Romney ram lambs were randomly allocated to one of three treatments: commercial milk replacer fed to 57 d of age and high fibre concentrate pellets (HFP57); commercial milk replacer, high fibre concentrate pellets, and early weaning from milk replacer at 42 d of age (HFP42); high protein milk replacer from 2–16 d of age followed by commercial milk replacer, low fibre concentrate pellets, and early weaning from milk replacer at 42 d of age (LFP42). Lambs were slaughtered at 57 d of age. Volatile fatty acid content in rumen fluid at slaughter was analysed and rumen tissue samples were collected for histological examination. The rumen n-butyric content was greater (p < 0.05) in both LFP42 and HFP42 treatment lambs compared to HFP57 lambs. The n-valeric content was greater (p < 0.05) in LFP42 lambs compared to both HFP57 and HFP42 treatment lambs. Thickness of the rumen dorsal wall determined by ultrasound scanning at 49 d was greater (p < 0.05) in both HFP42 and LFP42 lambs compared to HFP57 lambs. There was an interaction (p < 0.05) between treatment and site of rumen tissue sampling on papillae width, density, and rumen muscular layer thickness. Collectively, early weaning and the provision of a low fibre pellet leads to improved rumen function and physical development.

Dairy productivity of Kazakh horse mares

The work aimed to establish the influence of udder parameters of Kazakh mares on their formation of dairy productivity and the growth rate of foals during the milk period. The cup-shaped udder is characterized by a large base (the udder circumference at the base is 70.3 cm), the symmetrical arrangement of both udder lobes, with well-developed cylindrical nipples, the nipples are widely spaced between the two lobes. The round shaped udder is instead characterized by conical and short nipples and a smaller udder volume. For four months of lactation, 1051 liters of commercial milk were produced from mares with cup-shaped udder, while 823.6 liters- with round-shaped udder.The observation results showed that foals from mares with the cup-shaped and round - shapedudders on the first day of life were 39.8 and 39.6 kg of live weight, respectively, then after a month, it doubles, reaching 82.5 and 79.5 kg. The highest indicators of absolute gain - 42.7 kg, average daily gain - 1.42 kg, and relative gain -107.3% were observed in offspring with a cup-shaped udder. In foals delivered from mares with a round-shaped udder, the gain rates (39.9; 1.33 kg and 100.7%, respectively) were slightly lower.

Growth and Body Composition of Artificially-Reared Lambs Exposed to Three Different Rearing Regimens

This study was designed to investigate the influence of pellet fibre level, milk replacer composition and age at weaning on growth and body composition of lambs reared artificially. Romney ram lambs were randomly allocated to one of three rearing treatments; HFP57: commercial milk replacer to 57 days of age, and high fibre concentrate pellets; HFP42: commercial milk replacer with early weaning at 42 days of age, and high fibre concentrate pellets; LFP42: high protein milk replacer from 2–16 days of age followed by commercial milk replacer with early weaning at 42 days of age, and low fibre concentrate pellets. Lambs were slaughtered at 57 days of age. Overall average daily liveweight gain of lambs did not differ (p > 0.05) between treatments. Dressing out percentage, carcass weight, empty small intestine and omental fat were higher (p < 0.05) in HFP57 than in both HFP42 and LFP42 lambs. HFP42 and LFP42 lambs had heavier (p < 0.05) empty rumen weights. Whole body protein content was higher (p < 0.05) in HFP42 lambs compared to both HFP57 and LFP42 lambs. Fat content and daily fat deposition were greater (p < 0.05) in HFP57 lambs than HFP42 and LFP42 lambs. Weaning lambs at 42 days of age with provision of either low or high fibre concentrate pellets, resulted in similar growth rates, reduced whole body fat deposition and was a more cost-effective rearing regimen.

Beliefs and Norms Associated with the Use of Ultra-Processed Commercial Milk Formulas for Pregnant Women in Vietnam

Commercial milk formula for pregnant women (CMF-PW) is an expensive, ultra-processed food with a high concentration of sugar, the consumption of which may be linked to negative health outcomes. However, CMF-PWs are promoted as beneficial for pregnant women and lactating mothers as well as their children. To date, little is known about the factors associated with the use of CMF-PW among pregnant women. We performed this analysis to examine the association between the use of CMF-PW and related beliefs and norms among pregnant women in Vietnam. We interviewed 268 pregnant women in their second and third trimesters from two provinces and one municipality representing diverse communities in Vietnam. Multinomial (polytomous) logistic regression, structural equation modeling (SEM), and propensity score matching (PSM) analysis were used to examine associations between beliefs and social norms related to CMF-PW and reported consumption, characterized as occasional, recent, and never during the current pregnancy. Overall, 64.6% of pregnant women reported using CMF-PW during the current pregnancy and 34.7% consumed CMF-PW on the day prior to the interview. Strong beliefs that CMF-PW will make a child smart and healthy (53.7%) and the perception that use of CMF-PW is common (70.9%) were associated with increased use on the previous day (beliefs: aOR: 3.56; 95% Confidence Interval (95% CI): 1.65, 7.71; p < 0.01 and social norms aOR: 2.29; 95% CI: 1.13, 4.66; p < 0.05). SEM and PSM analyses confirmed these findings for both occasional and regular CMF-PW use. Results are consistent with observations of CMF-PW product labels and marketing tactics in Vietnam. The prevalent use of CMF-PW in Vietnam is associated with the belief that these products make children smart and healthy and the perceived social norm that most mothers use these products, which mirrors marketing messages and approaches employed by the CMF industry.

Projective mapping of preferences on milk and dark chocolate bar fortified nanocapsules Arthrospira carotenoid

The characteristics in milk or dark chocolate Arthrospira products require testing before the product is released to the market. The purpose of this study was to use the Projective Mapping (PM) method to determine the characteristics and preferences of consumers toward milk or dark chocolate Arthrospira carotenoid products compared to commercial milk or dark chocolate products on the market, and to analyze the factors that can influence consumers’ purchasing intention. The chocolate samples tested were Arthospira milk chocolate and ten commercial milk chocolate samples (SQ, DF, LD, DV, CB, MG, VH, BB, TB, and WD). The dark one compares to ten commercial dark chocolates (SQ, M5, M6, M7, DC, WD, TB, BB, LD, and DV). The factors that influence consumers’ purchasing intentions are identified through an online survey and analyzed using chi-square. A total of 159 people (68 PM respondents and 91 non-PM respondents) took part in the milk chocolate poll, while 92 people (41 PM respondents and 51 non-PM respondents) took part in the dark chocolate poll. PM test shows consumers perceive milk chocolate and dark chocolate fortified with nanocapsules Arthospira carotenoid to be similar in taste, appearance, texture, and price to some commercial chocolate bar products, implying that both chocolate products fortified with nanocapsulesArthospira carotenoid can compete in the market. The results of the chi-square test for milk chocolate show that for PM respondents, socio-demographic factors, consumer habits of buying chocolate bars, knowledge of Arthrospira, and the benefits of consuming Arthospira influence their buying interest, whereas for non-PM respondents, consumer habits of buying chocolate bars influence their buying intention. The results of the dark chocolate chi-square test show that respondents’ knowledge of the benefits of consuming chocolate bars and the emotion conducted to consume chocolate bars and influence their purchasing interest.

Implementation of the Code of Marketing of Breast-Milk Substitutes in Vietnam: Marketing Practices by the Industry and Perceptions of Caregivers and Health Workers

Background: The promotion of breastmilk substitutes (BMS) is an important barrier to successful breastfeeding. Objective: To examine the enactment and implementation of the Code of Marketing of Breast-Milk Substitutes (the Code) in Vietnam with a focus on marketing practices by the baby food industry and perceptions of caregivers, health workers, and policy makers. Methods: From May to July 2020, we conducted a mixed-method, cross-sectional study including a survey of 268 pregnant women and 726 mothers of infants aged 0–11 months and in-depth interviews with a subset of interviewed women (n = 39), policy makers, media executives, and health workers (n = 31). Results: In the previous 30 days, two mothers (out of 726) participating in the quantitative survey reported that health workers had recommended BMS, at private hospitals in both cases. In-depth interviews with health workers showed that hospitals have internal procedures to prevent the promotion of BMS by health workers. However, companies employed representatives to promote products not covered under the Code (e.g., commercial milk formula for pregnant women) at antenatal care visits and by gaining contact information from women and using this information to promote BMS outside the hospital, often on social media. In the 30 days preceding the survey, one-fifth of pregnant women were exposed to promotions of commercial milk formula for pregnant women and 7.1% to promotions of BMS. Among mothers of infants, 7.3% and 10.7% of respondents with infants aged 0–5 and 6–11 months, respectively, were exposed to some form of BMS promotion in the past 30 days. Around the time of birth, parents commonly brought BMS to maternity facilities (52.5%) or purchased it nearby (35.4%). Conclusions: Although Vietnam has a strong regulatory environment for the protection, promotion, and support of breastfeeding, there are implementation, monitoring, and enforcement gaps. Stronger enforcement of national policies to regulate the presence of BMS industry representatives in health facilities—both public and private—and the promotion of BMS products on digital platforms are needed.

Simultaneous Determination of C18 Fatty Acids in Milk by GC-MS

The determination of C18 fatty acids (FAs) is a key and difficult aspect in FA profiling, and a qualified method with good chromatographic separation and high sensitivity, as well as easy methylation, is required. A GC-MS method was established to simultaneously determine C18 FAs in milk. To simplify the methylation protocol for milk samples, besides a base-catalyzation methylation (50 °C for 20 min), the necessity of an additional acid-catalyzation was also studied using different temperatures (60 °C, 70 °C, 80 °C, and 90 °C) and durations (90 min and 150 min). The results showed that the chromatographic resolution was improved, although three co-eluted peaks existed. The base-catalyzation was sufficient, and an additional acid-catalyzation was not necessary. The proposed method was validated with good sensitivity, linearity, accuracy, and precision, and then applied in determining C18 FAs in 20 raw milk and 30 commercial milk samples. UHT milk presented a different profile of C18 FAs from raw milk and PAS milk samples, which indicated that excessive heating could change the profile. Overall, the proposed method is a high-throughput and competent approach for the determination of C18 FAs in milk, and which presents an improvement in chromatographic resolution and sensitivity, as well as a simplification of methylation.

Presence of Aflatoxin M1 in commercial milk in Paraguay

The presence of AFM 1 in milk is a public health concern, since milk is a significant part of human diets across nations. In economies where monitoring is low or non-existent, the possibility of contamination with AFM 1 might be increased. Our study aimed to detect and quantify the concentration of AFM 1 in fluid milk and milk drink of different brands, fat contents, packages and, heat treatments sold in the Metropolitan Area of Asunción. 80 samples were collected following a non-probability sampling method; acquired from supermarkets from the Metropolitan Area of Asunción. To quantify AFM 1 , the AgraQuant® ELISA Aflatoxin M 1 25 – 500 ppt was used. Non-parametric analysis was performed. All samples presented positive values for the detection limit (25 ng/kg); 85% of the samples showed values between 30 to 50 ng/kg, and 15% showed values over 500 ng/kg. There was no significant difference in the content of AFM 1 between fat contents, heat treatments, or types of packaging. However, brands differed significantly.

Influence of 2′-Fucosyllactose on the Microbiota Composition and Metabolic Activity of Fecal Cultures from Breastfed and Formula-Fed Infants at Two Months of Age

Although breast milk is considered the gold standard of nutrition for infant feeding, some circumstances may make breastfeeding difficult. Several commercial milk preparations include synthetic human milk oligosaccharides (HMOs) in their composition. However, the effect of HMOs on the establishment of the intestinal microbiota remains incompletely understood. Independent batch fermentations were performed with feces from six full-term infant donors of two months of age (three breastfed and three formula-fed, exclusively) in the presence of 2′fucosyllactose (2′FL), one of the most abundant HMOs in human milk. Microbiota composition was analyzed by 16S rRNA gene sequencing at baseline and at 24 h of incubation. The 2′FL consumption, gas accumulation, and levels of different metabolites were determined by chromatography. Microbiota profiles at baseline were clearly influenced by the mode of feeding and by the intrinsic ability of microbiotas to degrade 2′FL. The 2′FL degradation rate clustered fecal cultures into slow and fast degraders, regardless of feeding type, this being a determinant factor influencing the evolution of the microbiota during incubation, although the low number of donors precludes drawing sound conclusions. More studies are needed to decipher the extent to which the early intervention with HMOs could influence the microbiota as a function of its ability to utilize 2′FL.