Helminth Parasites of Conventionally Maintained Laboratory Mice: II- Inbred Strains with an Adaptation of the Anal Swab Technique

SummaryMice are the most widely used experimental mammals, and many inbred strains are available. However, except for the relatively recent strains derived from known wild populations, the relationships between wild and laboratory mice are not well understood. Based on the Y-chromosomal restriction fragmentlength polymorphism, seventeen inbred strains were classified into two groups: strains with the Mus musculus musculus type Y chromosome and those with the M. m. domesticus type Y chromosome. We extended the survey to an additional twenty-two inbred strains. The M. m. musculus type Y chromosome was found in AEJ/GnLe, AAU/SsJ, BDP/J, BXSB/MpJ, DA/HuSn, HTG/GoSfSn, I/LnJ, LP/J, NZW/LacJ, RIIIS/J, SB/Le, SEA/GnJ, SF/CamEi, SK/CamEi, SM/J, WB/ReJ, WC/ReJ and YBR/Ei, while the M. m. domesticus type Y chromosome was present in BUB/BnJ, MA/MyJ, PL/J and ST/bJ.

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Peer Review #3 of "Temporal dynamics of the developing lung transcriptome in three common inbred strains of laboratory mice reveals multiple stages of postnatal alveolar development (v0.1)"

10.7287/peerj.2318v0.1/reviews/3 ◽

2016 ◽

Author(s):

A Holik

Keyword(s):

Peer Review ◽

Temporal Dynamics ◽

Inbred Strains ◽

Laboratory Mice ◽

Alveolar Development ◽

Multiple Stages

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Temporal dynamics of the developing lung transcriptome in three common inbred strains of laboratory mice reveals multiple stages of postnatal alveolar development

PeerJ ◽

10.7717/peerj.2318 ◽

2016 ◽

Vol 4 ◽

pp. e2318 ◽

Cited By ~ 29

Author(s):

Kyle J. Beauchemin ◽

Julie M. Wells ◽

Alvin T. Kho ◽

Vivek M. Philip ◽

Daniela Kamir ◽

...

Keyword(s):

Gene Expression ◽

Lung Development ◽

Transcriptional Activity ◽

Expression Patterns ◽

Inbred Strains ◽

Expression Data ◽

Laboratory Mice ◽

Alveolar Development ◽

Matrix Organization ◽

Strain Dependent

To characterize temporal patterns of transcriptional activity during normal lung development, we generated genome wide gene expression data for 26 pre- and post-natal time points in three common inbred strains of laboratory mice (C57BL/6J, A/J, and C3H/HeJ). Using Principal Component Analysis and least squares regression modeling, we identified both strain-independent and strain-dependent patterns of gene expression. The 4,683 genes contributing to the strain-independent expression patterns were used to define a murine Developing Lung Characteristic Subtranscriptome (mDLCS). Regression modeling of the Principal Components supported the four canonical stages of mammalian embryonic lung development (embryonic, pseudoglandular, canalicular, saccular) defined previously by morphology and histology. For postnatal alveolar development, the regression model was consistent with four stages of alveolarization characterized by episodic transcriptional activity of genes related to pulmonary vascularization. Genes expressed in a strain-dependent manner were enriched for annotations related to neurogenesis, extracellular matrix organization, and Wnt signaling. Finally, a comparison of mouse and human transcriptomics from pre-natal stages of lung development revealed conservation of pathways associated with cell cycle, axon guidance, immune function, and metabolism as well as organism-specific expression of genes associated with extracellular matrix organization and protein modification. The mouse lung development transcriptome data generated for this study serves as a unique reference set to identify genes and pathways essential for normal mammalian lung development and for investigations into the developmental origins of respiratory disease and cancer. The gene expression data are available from the Gene Expression Omnibus (GEO) archive (GSE74243). Temporal expression patterns of mouse genes can be investigated using a study specific web resource (http://lungdevelopment.jax.org).

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Post-stress chemosignals affect cells from immunocompetent organs in laboratory mice of three inbred strains

Ecological genetics ◽

10.17816/ecogen6127-33 ◽

2008 ◽

Vol 6 (1) ◽

pp. 27-33 ◽

Cited By ~ 1

Author(s):

Eugene V Daev ◽

Boris P Surinov ◽

Anna V Dukelskaya

Keyword(s):

Bone Marrow ◽

Bone Marrow Cells ◽

Physiological State ◽

Inbred Strains ◽

Laboratory Mice ◽

Immunocompetent Organs ◽

Dividing Cells ◽

First Time ◽

Mitotic Disturbances ◽

Post Stress

Quantity of antibody producing cells and changes in bone marrow dividing cells of mouse males were studied after the exposure with chemosignals from intact or stressed donor mouse males. Inbred CBA, BALB/c and C57BL/6 strains were used. It is shown that excreted volatiles decrease quantity of antibody producing cells in spleen and at the same time raise the level of mitotic disturbances in bone marrow cells of recipient mice. Pheromone effect depends on genotype and physiological state of the recipients. For the first time we describe here the influence of mouse female pheromone 2,5-dimethylpyrazine on analyzed features. Biological meaning of the discovered effects is discussed.

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