scholarly journals Selection of Trypanosoma cruzi clonal genotypes (clonet 20 and 39) isolated from Bolivian triatomines following subculture in liquid medium

2000 ◽  
Vol 95 (5) ◽  
pp. 601-607 ◽  
Author(s):  
Marie-France Bosseno ◽  
Nina Yacsik ◽  
Fernando Vargas ◽  
Simone Frédérique Brenière
Keyword(s):  
Trypanosoma Cruzi ◽  
Liquid Medium ◽  
Selection Of

The role of calcium in the increase in flocculence of yeast growing in the presence of copper

1965 ◽  
Vol 162 (989) ◽  
pp. 555-566 ◽  
Keyword(s):  
Liquid Medium ◽  
Cell Cultures ◽  
Chloride Solution ◽  
Liquid Copper ◽  
Acquired Resistance ◽  
Calcium Chloride Solution ◽  
Copper Resistance ◽  
Liquid Cultures ◽  
Selection Of

Growth in the presence of inhibitory concentrations of copper enhances the tendency of yeast to flocculate. Many yeasts will not flocculate unless calcium is included in the growth medium and Guinness strain 522 used in the present work required a relatively large amount. Single cell cultures may undergo variation during subculture, resulting in the production of a large number of variants (Chester 1963). The cells of these variants differ considerably in their ability to adhere together. Flocculation variants of strain 522 differed among themselves in the amount of calcium necessary for flocculation, the most flocculent variants requiring least calcium. Washed cells of the more flocculent yeasts removed more calcium from a calcium chloride solution than did those with lesser powers of adhesion. In a copper medium con­taining calcium the more flocculent variants replaced the less flocculent. Calcium protected cells from copper and the more flocculent variants enjoyed most protection. All variants acquired resistance to copper during growth in the copper medium. Despite the selection of the more flocculent yeasts during growth in liquid medium, their copper resistance was less than that of the less flocculent yeasts. When calcium was added to the liquid copper medium, cultures developed less resistance. It is concluded that the less flocculent cells, having less protection by calcium, were exposed to what was effectively a greater concentration of copper and therefore became more resistant. This greater resistance did not enable these cells to compete with the flocculent cells in liquid cultures.

scholarly journals Trypanosoma cruzi: cell charge distribution

1985 ◽  
Vol 27 (6) ◽  
pp. 312-317 ◽  
Author(s):  
T. Langenbach
Keyword(s):  
Trypanosoma Cruzi ◽  
Charge Distribution ◽  
Surface Coat ◽  
Blood Components ◽  
Host Blood ◽  
Selection Of

Differences in cell charge between epimastigote and trypomastigote populations were compared in Y, Cl and Colombiana strains of T. cruzi. Trypomastigote populations were more homogenous in relation to cell charge than epimastigotes. This homogeneity of cell charge was not the result of the selection of trypomastigote sub-populations by the host immunosystem, but may be the result of a surface coat formed by host blood components.

scholarly journals Genotyping of Trypanosoma cruzi: Systematic Selection of Assays Allowing Rapid and Accurate Discrimination of All Known Lineages

2009 ◽  
Vol 81 (6) ◽  
pp. 1041-1049 ◽  
Author(s):  
Michael D. Lewis ◽  
Hernán J. Carrasco ◽  
Matthew Yeo ◽  
Jonathan Ma ◽  
Michael A. Miles ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Systematic Selection ◽  
Selection Of

pRIBOTEX expression vector: a pTEX derivative for a rapid selection of Trypanosoma cruzi transfectants

Gene ◽  
1997 ◽  
Vol 199 (1-2) ◽  
pp. 71-76 ◽  
Author(s):  
Santiago Martı́nez-Calvillo ◽  
Imelda López ◽  
Roberto Hernández
Keyword(s):  
Trypanosoma Cruzi ◽  
Expression Vector ◽  
Selection Of

IN VITRO PRODUCTION OF ERGOT ALKALOIDS BY CULTURES OF CLAVICEPS PURPUREA (FR.) TUL.

1957 ◽  
Vol 3 (1) ◽  
pp. 55-60 ◽  
Author(s):  
W. A. Taber ◽  
L. C. Vining
Keyword(s):  
Liquid Medium ◽  
Incubation Period ◽  
Ergot Alkaloids ◽  
Claviceps Purpurea ◽  
In Vitro Production ◽  
Factors Influencing ◽  
Ammonium Succinate ◽  
Vitro Production ◽  
Selection Of

Stationary cultures of Claviceps purpurea (Fr.) Tul. grown 50 days on a liquid medium containing mannitol, ammonium succinate, and salts produced small amounts of ergot alkaloids.Ergometrine, ergometrinine, ergotamine, ergotaminine, ergocornine, ergocorninine, ergocryptine, agroclavine, and penniclavine were identified by paper chromatography of culture extracts. The selection of strains, the composition of the medium, and the length of the incubation period appeared to be important factors influencing the production of alkaloids.

In vivo selection of a population of Trypanosoma cruzi and clones resistant to benznidazole

Parasitology ◽  
1998 ◽  
Vol 116 (2) ◽  
pp. 165-171 ◽  
Author(s):  
S. M. F. MURTA ◽  
A. J. ROMANHA
Keyword(s):  
Mortality Rate ◽  
Trypanosoma Cruzi ◽  
Type Strain ◽  
Wild Type Strain ◽  
Wild Type ◽  
Long Term Treatment ◽  
Resistant Population ◽  
Parasitaemia Level ◽  
Selection Of

A benznidazole-resistant population of Trypanosoma cruzi, Y strain, was selected after 25 successive passages (8 months) in mice treated with a single high drug dose. Initially, the resistant parasites produced a low parasitaemia level and low mortality rate in infected mice. Thereafter, the parasitaemia level and mortality rate increased to the same value obtained for mice infected with the wild-type strain. Long-term treatment with benznidazole (100 mg/kg/day) cured 71–80% of mice infected with the wild-type strain. No cure was observed in mice infected with the selected resistant parasite population. Treatment with 500 mg/kg of benznidazole at peak parasitaemia cleared all blood parasites from mice infected with wild-type parasites. No effect on parasitaemia level was observed in mice infected with the selected parasites. Benznidazole-resistant parasites showed cross-resistance to different drugs. Contrary to wild type, all clones analysed from the resistant T. cruzi population were resistant to benznidazole. Without drug pressure the resistance phenotype of clones was far more stable than that presented by the resistant population. This work demonstrates, for the first time, the in vivo selection of a population and clones of T. cruzi resistant to benznidazole, and makes available an experimental model for the study of mechanisms of drug resistance in T. cruzi.

Effect of antibiotics on cell proliferation in the Closterium peracerosum-strigosum-littorale complex (Charophyceae, Chlorophyta)

Biologia ◽  
2008 ◽  
Vol 63 (6) ◽  
Author(s):  
Jun Abe ◽  
Kaori Sakayori ◽  
Hiroyuki Sekimoto
Keyword(s):  
Cell Proliferation ◽  
Liquid Medium ◽  
Inhibitory Concentration ◽  
Solid Medium ◽  
Marker Genes ◽  
Hygromycin B ◽  
Selectable Marker Genes ◽  
Kanamycin Sulfate ◽  
Low Concentrations ◽  
Selection Of

AbstractThe effects of several antibiotics on the proliferation of cells of the Closterium peracerosum-strigosum-littorale complex, a unicellular charophycean alga, were examined. When cells were cultured on solid medium containing hygromycin B and phleomycin the proliferation of cells was inhibited at low concentrations of these antibiotics, with a minimum inhibitory concentration of 5.0 and 0.2 µg/mL, respectively. By contrast, kanamycin sulfate was less effective at concentrations up to 50 µg/mL. When cells were incubated in liquid medium containing hygromycin B and phleomycin, cell proliferation was severely inhibited at concentrations of 5.0 and 0.01 µg/mL, respectively. It is concluded that hygromycin B and phleomycin are highly effective for inhibiting the proliferation of C. psl. complex both on solid and in liquid medium and thus are useful for the selection of the cells transformed by selectable marker genes.

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