scholarly journals Trypanosoma cruzi: cell charge distribution

1985 ◽  
Vol 27 (6) ◽  
pp. 312-317 ◽  
Author(s):  
T. Langenbach
Keyword(s):  
Trypanosoma Cruzi ◽  
Charge Distribution ◽  
Surface Coat ◽  
Blood Components ◽  
Host Blood ◽  
Selection Of

Differences in cell charge between epimastigote and trypomastigote populations were compared in Y, Cl and Colombiana strains of T. cruzi. Trypomastigote populations were more homogenous in relation to cell charge than epimastigotes. This homogeneity of cell charge was not the result of the selection of trypomastigote sub-populations by the host immunosystem, but may be the result of a surface coat formed by host blood components.

scholarly journals Selection of Blood, Blood Components, and Blood Products as Essential Medicines in 105 Low- and Middle-Income Countries

2020 ◽  
Vol 34 (2) ◽  
pp. 94-100
Author(s):  
Washington T. Samukange ◽  
Helga Gardarsdottir ◽  
Hubert G.M. Leufkens ◽  
Aukje K. Mantel-Teeuwisse
Keyword(s):  
Essential Medicines ◽  
Blood Components ◽  
Blood Products ◽  
Middle Income ◽  
Middle Income Countries ◽  
Selection Of ◽  
Low And Middle Income

scholarly journals Selection of Trypanosoma cruzi clonal genotypes (clonet 20 and 39) isolated from Bolivian triatomines following subculture in liquid medium

2000 ◽  
Vol 95 (5) ◽  
pp. 601-607 ◽  
Author(s):  
Marie-France Bosseno ◽  
Nina Yacsik ◽  
Fernando Vargas ◽  
Simone Frédérique Brenière
Keyword(s):  
Trypanosoma Cruzi ◽  
Liquid Medium ◽  
Selection Of

scholarly journals Genotyping of Trypanosoma cruzi: Systematic Selection of Assays Allowing Rapid and Accurate Discrimination of All Known Lineages

2009 ◽  
Vol 81 (6) ◽  
pp. 1041-1049 ◽  
Author(s):  
Michael D. Lewis ◽  
Hernán J. Carrasco ◽  
Matthew Yeo ◽  
Jonathan Ma ◽  
Michael A. Miles ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Systematic Selection ◽  
Selection Of

INTERPRETATION OF THE RESULTS OF IMMUNOHEMATOLOGICAL TESTS IN HEMATOLOGICAL PATIENTS

2019 ◽  
Vol 64 (4) ◽  
pp. 221-224
Author(s):  
A. V. Yovdiy ◽  
E. V. Butina ◽  
E. A. Poponina ◽  
G. A. Zaitseva ◽  
N. V. Minaeva
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Components ◽  
Blood Types ◽  
Hematological Patients ◽  
Correct Determination ◽  
Double Population ◽  
Transfusion Complications ◽  
Selection Of

The correct determination of the blood types of the recipient and the donor is very importante for the choice of blood components for transfusion. As a result of the study, it was established that 18.0% of patients, admitted to the hematology clinic, have difficulties in interpreting of the results of immunohematological tests. Most often, a double population of red blood cells was detected when determining antigens of the Rhesus system (10.9%), auto- (3.9%) and alloantibodies (2.8%). The proposed algorithm for the selection of donor red blood cells in difficult diagnostic cases helps to prevent the development of post-transfusion complications.

pRIBOTEX expression vector: a pTEX derivative for a rapid selection of Trypanosoma cruzi transfectants

Gene ◽  
1997 ◽  
Vol 199 (1-2) ◽  
pp. 71-76 ◽  
Author(s):  
Santiago Martı́nez-Calvillo ◽  
Imelda López ◽  
Roberto Hernández
Keyword(s):  
Trypanosoma Cruzi ◽  
Expression Vector ◽  
Selection Of

An unusual structural organization to the gut of a digenetic trematode, Fellodistomum fellis

Parasitology ◽  
1982 ◽  
Vol 85 (1) ◽  
pp. 53-60 ◽  
Author(s):  
D. W. Halton
Keyword(s):  
Cell Surface ◽  
Elemental Analysis ◽  
Structural Organization ◽  
Digenetic Trematode ◽  
Blood Components ◽  
Ultrastructural Examination ◽  
Anterior Portion ◽  
Digestive Cells ◽  
Histochemical Tests ◽  
Host Blood

SUMMARYAn ultrastructural examination of Fellodistomum fellis has revealed that the caecal lining consists of 2 distinct components: (a) cup-shaped digestive cells and (b) a pleomorphic layer of cytoplasm which supports and separates individual digestive cells. The digestive cells sequester host blood components within apical pockets formed by lamellated extensions of the cell surface, and undergo asynchronous, cyclical transformations in morphology associated with extracellular digestion and with the extrusion to the gut lumen of pigmented digestive residues. Histochemical tests and elemental analysis of the pigment suggest that it is a ferripor-phyrin, haematin. In the anterior portion of the caecum the supporting cytoplasmic layer is in continuity with the oesophageal tegument and snares the same ultrastructure. It is concluded that the digestive cells are supported by an extension of the foregut tegument.

INACTIVATION OF Trypanosoma cruzi TRYPOMASTIGOTE FORMS IN BLOOD COMPONENTS BY PHOTODYNAMIC TREATMENT WITH PHTHALOCYANINES

2008 ◽  
Vol 62 (5) ◽  
pp. 869-874 ◽  
Author(s):  
Paul Gottlieb ◽  
Li-Gang Shen ◽  
Eleanor Chimezie ◽  
Soon Bahng ◽  
Malcolm E. Kenney ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Blood Components ◽  
Photodynamic Treatment

In vivo selection of a population of Trypanosoma cruzi and clones resistant to benznidazole

Parasitology ◽  
1998 ◽  
Vol 116 (2) ◽  
pp. 165-171 ◽  
Author(s):  
S. M. F. MURTA ◽  
A. J. ROMANHA
Keyword(s):  
Mortality Rate ◽  
Trypanosoma Cruzi ◽  
Type Strain ◽  
Wild Type Strain ◽  
Wild Type ◽  
Long Term Treatment ◽  
Resistant Population ◽  
Parasitaemia Level ◽  
Selection Of

A benznidazole-resistant population of Trypanosoma cruzi, Y strain, was selected after 25 successive passages (8 months) in mice treated with a single high drug dose. Initially, the resistant parasites produced a low parasitaemia level and low mortality rate in infected mice. Thereafter, the parasitaemia level and mortality rate increased to the same value obtained for mice infected with the wild-type strain. Long-term treatment with benznidazole (100 mg/kg/day) cured 71–80% of mice infected with the wild-type strain. No cure was observed in mice infected with the selected resistant parasite population. Treatment with 500 mg/kg of benznidazole at peak parasitaemia cleared all blood parasites from mice infected with wild-type parasites. No effect on parasitaemia level was observed in mice infected with the selected parasites. Benznidazole-resistant parasites showed cross-resistance to different drugs. Contrary to wild type, all clones analysed from the resistant T. cruzi population were resistant to benznidazole. Without drug pressure the resistance phenotype of clones was far more stable than that presented by the resistant population. This work demonstrates, for the first time, the in vivo selection of a population and clones of T. cruzi resistant to benznidazole, and makes available an experimental model for the study of mechanisms of drug resistance in T. cruzi.

scholarly journals Structure Activity Relationship of N-Substituted Phenyldihydropyrazolones Against Trypanosoma cruzi Amastigotes

2021 ◽  
Vol 9 ◽  
Author(s):  
Maarten Sijm ◽  
Louis Maes ◽  
Iwan J. P. de Esch ◽  
Guy Caljon ◽  
Geert Jan Sterk ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Drug Effects ◽  
Treatment Compliance ◽  
Treatment Regimens ◽  
Poor Treatment ◽  
Sar Study ◽  
New Compounds ◽  
Relationship Of ◽  
Selection Of

Current drugs for Chagas disease have long treatment regimens with occurrence of adverse drug effects leading to poor treatment compliance. Novel and efficacious medications are therefore highly needed. We previously reported on the discovery of NPD-0227 (2-isopropyl-5-(4-methoxy-3-(pyridin-3-yl)phenyl)-4,4-dimethyl-2,4-dihydro-3H-pyrazol-3-one) as a potent in vitro inhibitor of Trypanosoma cruzi (pIC50 = 6.4) with 100-fold selectivity over human MRC-5 cells. The present work describes a SAR study on the exploration of substituents on the phenylpyrazolone nitrogen. Modifications were either done directly onto this pyrazolone nitrogen or alternatively by introducing a piperidine linker. Attention was pointed toward the selection of substituents with a cLogP preferably below NPD-0227s cLogP of 3.5. Generally the more apolar compounds showed better activities then molecules with cLogPs <2.0. Several new compounds were identified with potencies that are in the same range as NPD-0227 (pIC50 = 6.4) and promising selectivities. While the potency could not be improved, valuable SAR was obtained. Furthermore the introduction of a piperidine linker offers new opportunities for derivatization as valuable novel starting points for future T. cruzi drug discovery.

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