scholarly journals Susceptibilidade antimicrobiana de Campylobacter fetus subsp. venerealis isolado de bovinos

2005 ◽  
Vol 25 (1) ◽  
pp. 1-3
Author(s):  
Agueda C. Vargas ◽  
Mateus M. Costa ◽  
Ana C.M. Groff ◽  
Luciane R. Viana ◽  
Cristina C. Krewer ◽  
...  
Keyword(s):  
Campylobacter Fetus ◽  
Fetus Subsp

A campilobacteriose venérea bovina, ocasionada principalmente pelo Campylobacter fetus subsp. fetus e Campylobacter subsp. venerealis, é transmitida através do coito ou por inseminação com sêmen contaminado. O propósito deste estudo foi determinar a susceptibilidade in vitro de isolados de C. fetus subesp. venerealis a agentes antimicrobianos comumente utilizados para o tratamento clínico e de sêmen. Foram testadas duas cepas padrão, sendo uma de C. fetus subsp. fetus e outra de C. fetus subsp. venerealis, bem como 21 amostras de isolados clínicos de C. fetus subsp. venerealis. Os testes foram realizados conforme o método de Kirby-Bauer. A amostra padrão de C. fetus subsp. fetus demonstrou-se resistente à lincomicina, penicilina e ácido nalidíxico, enquanto a de C. fetus subsp. venerealis apresentou susceptibilidade a todos antimicrobianos testados, com exceção do ácido nalidíxico. Todas as amostras de C. fetus subsp. venerealis foram susceptíveis à amicacina, ampicilina, cefalotina, estreptomicina, gentamicina, penicilina e tetraciclina. Foi observada resistência de 42,86% à lincomicina e 4,76 % a enrofloxacina, e de 100% ao ácido nalidíxico. Ainda, 4,76% apresentaram susceptibilidade intermediária à enrofloxacina, neomicina e polimixina B e 9,52% à lincomicina. Os resultados evidenciaram a sensibilidade das amostras analisadas aos antimicrobianos comumente utilizados para o tratamento clínico e do sêmen.

In vitro Fertilization of Bovine Ova in the Presence of Campylobacter fetus subsp. venerealis

1994 ◽  
Vol 29 (6) ◽  
pp. 488-493 ◽  
Author(s):  
A. Bielanski ◽  
M. Sampath ◽  
C. Gradil ◽  
M. D. Eaglesome ◽  
M. Garcia
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Campylobacter Fetus ◽  
Fetus Subsp

TheCampylobacter fetusS layer is not essential for initial interaction with HEp-2 cells

1998 ◽  
Vol 44 (3) ◽  
pp. 244-250 ◽  
Author(s):  
Lori L Graham ◽  
K L MacDonald
Keyword(s):  
Wild Type ◽  
Cell Numbers ◽  
Campylobacter Fetus ◽  
Time Period ◽  
Significant Difference ◽  
Mutant Strains ◽  
Fetus Subsp ◽  
Initial Interaction ◽  
Wild Type Parent

In vitro adherence assays were used to determine whether the S layer mediated interactions between Campylobacter fetus subsp. venerealis strains and HEp-2 cells. At multiplicity of infection ratios ranging from 0.1:1 through 100:1, quantitation of bacterial adherence by light microscopy revealed that S layer deficient isogenic C. fetus 809K and C. fetus 810K were not less efficient in their attachment to HEp-2 cells; either S layer deficient C. fetus strains interacted with HEp-2 cells in greater numbers than the corresponding wild-type parent strains 809 and 810 or there was no significant difference in adherence levels between wild-type and mutant strains. Adherence of C. fetus strains to HEp-2 cells increased most during the first 2 h of a 22-h incubation period with only a slight increase in C. fetus cell numbers occuring subsequent to 2 h. At each assay point throughout this 22-h time period, equivalent numbers of wild-type and S layer deficient C. fetus strains were observed associated with HEp-2 cells. Prior to 2 h, adherence levels of all C. fetus strains exceeded those of Escherichia coli AB264 and Salmonella typhimurium SL1344. And, unlike S. typhimurium, C. fetus did not undergo significant replication following initial adherence to HEp-2 cells. Campylobacter fetus did not adhere to HEp-2 cells in a localized or aggregative pattern but were randomly distributed over individual HEp-2 cells and at no time during the assay with C. fetus were changes in HEp-2 cell morphology apparent. These data suggest that the S layer is not essential for mediating initial interactions between C. fetus and HEp-2 cells.Key words: Campylobacter fetus, S layer, HEp-2.

scholarly journals In vitro susceptibilities of 40 Campylobacter fetus subsp. jejuni strains to niridazole and metronidazole.

1984 ◽  
Vol 25 (1) ◽  
pp. 145-146 ◽  
Author(s):  
A M Freydiere ◽  
Y Gille ◽  
S Tigaud ◽  
P Vincent
Keyword(s):  
Campylobacter Fetus ◽  
Fetus Subsp

scholarly journals In vitro susceptibility of Campylobacter fetus subsp. jejuni to N-formimidoyl thienamycin, rosaramicin, cefoperazone, and other antimicrobial agents.

1981 ◽  
Vol 20 (6) ◽  
pp. 850-851 ◽  
Author(s):  
V I Ahonkhai ◽  
C E Cherubin ◽  
M F Sierra ◽  
V D Bokkenheuser ◽  
M A Shulman ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
In Vitro Susceptibility ◽  
Campylobacter Fetus ◽  
Fetus Subsp

scholarly journals Genomic and Phenotypic Characterization of Campylobacter fetus subsp. venerealis Strains

2021 ◽  
Vol 9 (2) ◽  
pp. 340
Author(s):  
Marta F. Silva ◽  
Ana L. Pereira ◽  
Maria J. Fraqueza ◽  
Gonçalo Pereira ◽  
Luísa Mateus ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Etiologic Agent ◽  
Phenotypic Characterization ◽  
Putative Virulence Factor ◽  
Whole Genome ◽  
Campylobacter Fetus ◽  
Virulence Potential ◽  
Fetus Subsp ◽  
Bovine Genital Campylobacteriosis

The pathogenesis mechanisms of Campylobacter fetus subsp. venerealis (Cfv), the etiologic agent of Bovine Genital Campylobacteriosis remain elusive. This study evaluated the virulence potential and biovar characteristics of Cfv isolates (n = 13) by PCR screening of putative virulence-factor (VF) genes, Multilocus Sequence Typing (MLST) analysis, antimicrobial susceptibility to tetracycline, penicillin, enrofloxacin and streptomycin testing and whole-genome sequencing (WGS; n = 5), also comparing the latter with 26 other whole-genome sequences of Cfv strains. The putative VF genes encoding type IV secretion system of Cfv (virB2-virB11/virD4) were absent in 92% of isolates, including isolates from aborted foetuses, evidencing that these VF genes are not essential for Cfv pathogenicity. The parA gene, used as a Cfv diagnostic molecular target, was detected in only 3 of 13 isolates, invalidating its use for diagnosis purposes. Three novel sequence types were identified by MLST. Although no in vitro antimicrobial resistance was detected, WGS identified antimicrobial resistance-related genes, including those encoding the multidrug efflux pumps CmeABC and YkkCD, indicating that their presence is not enough to provide antimicrobial resistance. The SNP and accessory protein families analysis segregated the Cfv and Cfv biovar intermedius (Cfvi) strains into different clusters. In conclusion, this study evidenced virulence potential and biovar characteristics of Cfv and Cfvi, which are of relevance for the control of Bovine Genital Campylobacteriosis.

IMMUNISATION AGAINST BOVINE VIBRIOSIS DUE TO CAMPYLOBACTER FETUS SUBSP. FETUS BIOTYPE INTERMEDIUS

1976 ◽  
Vol 52 (8) ◽  
pp. 362-365 ◽  
Author(s):  
B. L. Clark ◽  
J. H. Dufty ◽  
Mary J. Monsbourgh ◽  
I. M. Parsonson
Keyword(s):  
Campylobacter Fetus ◽  
Fetus Subsp

scholarly journals Influence of Campylobacter fetus subsp. fetus on ram sperm cell quality

2008 ◽  
Vol 57 (11) ◽  
pp. 1405-1410 ◽  
Author(s):  
Tidhar Zan Bar ◽  
Ronen Yehuda ◽  
Tomer Hacham ◽  
Sigal Krupnik ◽  
Benjamin Bartoov
Keyword(s):  
Sperm Cell ◽  
Sperm Cells ◽  
Male Factor ◽  
Motility Index ◽  
Fas Receptor ◽  
Campylobacter Fetus ◽  
Fetus Subsp ◽  
Ram Sperm ◽  
Female Sheep

Campylobacter fetus subsp. fetus infection can occur in female sheep, causing infertility or abortion. Despite extensive research on the effect of these bacteria on female fertility, little research has been done on the influence of C. fetus subsp. fetus on the male factor. Our objective was to examine the influence of C. fetus subsp. fetus on ram sperm. Motility index, percentage of live spermatozoa, mean αt value (an indication of the chromatin stability of the sperm cell) and percentage of sperm cells expressing the FAS receptor were measured in sperm incubated in the presence or absence of C. fetus subsp. fetus. The motility index and viability of sperm incubated with the bacteria were lower than those of untreated sperm samples after 5 h. In bacteria-incubated sperm cells, the percentage expressing FAS receptor was already significantly elevated at 15 min. Bacteria-incubated sperm showed a greater prevalence of morphological damage. The bacteria were attached to tail and acrosome regions, and the sperm damage was concentrated in both the motility and chromatin regions. Bacteria-infected sperm cells showed a decrease in motility, increase in early acrosome reaction and chromatin damage. Similar effects were induced by incubation of the sperm with supernatants from C. fetus subsp. fetus cultures. Thus this study demonstrates that C. fetus subsp. fetus has a detrimental effect on the quality of ram sperm.

scholarly journals Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display

2021 ◽  
Vol 51 (8) ◽  
Author(s):  
Eduardo Lara Ribeiro ◽  
Patrícia Tiemi Fujimura ◽  
Carlos Ueira-Vieira ◽  
Luiz Ricardo Goulart ◽  
Telma Maria Alves ◽  
...  
Keyword(s):  
Phage Display ◽  
Phage Library ◽  
Hyperimmune Serum ◽  
Subclinical Disease ◽  
Campylobacter Fetus ◽  
Fetus Subsp ◽  
Cattle Herds ◽  
Cervicovaginal Mucus ◽  
Bovine Genital Campylobacteriosis

ABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal and subclinical disease that affects the fertility of cattle herds, and it is caused by Campylobacter fetus subsp. venerealis . This study selected peptides mimetic to the BGC-causing agent from a phage library. Phage display is a technique that applies bacteriophage libraries that reveal peptides fused to the viral capsid in biological selections against target proteins. Biopannings were performed for biological selection in the phage library using rabbit hyperimmune serum and C. fetus subsp. venerealis protein extract. Five selected heptapeptides were considered mimetic to Cfv-NCTC 10354 based on the results of bioinformatics analysis and assays with hyperimmune serum and cervicovaginal mucus obtained from heifers. ALASLPL and LSYLFPP were the most reactive peptides and considered promising as possible mimetic immunogens for C. fetus subsp. venerealis.

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