scholarly journals Genomic and Phenotypic Characterization of Campylobacter fetus subsp. venerealis Strains

2021 ◽  
Vol 9 (2) ◽  
pp. 340
Author(s):  
Marta F. Silva ◽  
Ana L. Pereira ◽  
Maria J. Fraqueza ◽  
Gonçalo Pereira ◽  
Luísa Mateus ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Etiologic Agent ◽  
Phenotypic Characterization ◽  
Putative Virulence Factor ◽  
Whole Genome ◽  
Campylobacter Fetus ◽  
Virulence Potential ◽  
Fetus Subsp ◽  
Bovine Genital Campylobacteriosis

The pathogenesis mechanisms of Campylobacter fetus subsp. venerealis (Cfv), the etiologic agent of Bovine Genital Campylobacteriosis remain elusive. This study evaluated the virulence potential and biovar characteristics of Cfv isolates (n = 13) by PCR screening of putative virulence-factor (VF) genes, Multilocus Sequence Typing (MLST) analysis, antimicrobial susceptibility to tetracycline, penicillin, enrofloxacin and streptomycin testing and whole-genome sequencing (WGS; n = 5), also comparing the latter with 26 other whole-genome sequences of Cfv strains. The putative VF genes encoding type IV secretion system of Cfv (virB2-virB11/virD4) were absent in 92% of isolates, including isolates from aborted foetuses, evidencing that these VF genes are not essential for Cfv pathogenicity. The parA gene, used as a Cfv diagnostic molecular target, was detected in only 3 of 13 isolates, invalidating its use for diagnosis purposes. Three novel sequence types were identified by MLST. Although no in vitro antimicrobial resistance was detected, WGS identified antimicrobial resistance-related genes, including those encoding the multidrug efflux pumps CmeABC and YkkCD, indicating that their presence is not enough to provide antimicrobial resistance. The SNP and accessory protein families analysis segregated the Cfv and Cfv biovar intermedius (Cfvi) strains into different clusters. In conclusion, this study evidenced virulence potential and biovar characteristics of Cfv and Cfvi, which are of relevance for the control of Bovine Genital Campylobacteriosis.

scholarly journals Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display

2021 ◽  
Vol 51 (8) ◽  
Author(s):  
Eduardo Lara Ribeiro ◽  
Patrícia Tiemi Fujimura ◽  
Carlos Ueira-Vieira ◽  
Luiz Ricardo Goulart ◽  
Telma Maria Alves ◽  
...  
Keyword(s):  
Phage Display ◽  
Phage Library ◽  
Hyperimmune Serum ◽  
Subclinical Disease ◽  
Campylobacter Fetus ◽  
Fetus Subsp ◽  
Cattle Herds ◽  
Cervicovaginal Mucus ◽  
Bovine Genital Campylobacteriosis

ABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal and subclinical disease that affects the fertility of cattle herds, and it is caused by Campylobacter fetus subsp. venerealis . This study selected peptides mimetic to the BGC-causing agent from a phage library. Phage display is a technique that applies bacteriophage libraries that reveal peptides fused to the viral capsid in biological selections against target proteins. Biopannings were performed for biological selection in the phage library using rabbit hyperimmune serum and C. fetus subsp. venerealis protein extract. Five selected heptapeptides were considered mimetic to Cfv-NCTC 10354 based on the results of bioinformatics analysis and assays with hyperimmune serum and cervicovaginal mucus obtained from heifers. ALASLPL and LSYLFPP were the most reactive peptides and considered promising as possible mimetic immunogens for C. fetus subsp. venerealis.

In vitro Fertilization of Bovine Ova in the Presence of Campylobacter fetus subsp. venerealis

1994 ◽  
Vol 29 (6) ◽  
pp. 488-493 ◽  
Author(s):  
A. Bielanski ◽  
M. Sampath ◽  
C. Gradil ◽  
M. D. Eaglesome ◽  
M. Garcia
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Campylobacter Fetus ◽  
Fetus Subsp

scholarly journals Whole-Genome Sequencing for Detecting Antimicrobial Resistance in Nontyphoidal Salmonella

2016 ◽  
Vol 60 (9) ◽  
pp. 5515-5520 ◽  
Author(s):  
Patrick F. McDermott ◽  
Gregory H. Tyson ◽  
Claudine Kabera ◽  
Yuansha Chen ◽  
Cong Li ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Resistance Genes ◽  
The United States ◽  
Whole Genome ◽  
Content Type ◽  
Structural Resistance ◽  
Retail Meat

ABSTRACTLaboratory-basedin vitroantimicrobial susceptibility testing is the foundation for guiding anti-infective therapy and monitoring antimicrobial resistance trends. We used whole-genome sequencing (WGS) technology to identify known antimicrobial resistance determinants among strains of nontyphoidalSalmonellaand correlated these with susceptibility phenotypes to evaluate the utility of WGS for antimicrobial resistance surveillance. Six hundred fortySalmonellaof 43 different serotypes were selected from among retail meat and human clinical isolates that were tested for susceptibility to 14 antimicrobials using broth microdilution. The MIC for each drug was used to categorize isolates as susceptible or resistant based on Clinical and Laboratory Standards Institute clinical breakpoints or National Antimicrobial Resistance Monitoring System (NARMS) consensus interpretive criteria. Each isolate was subjected to whole-genome shotgun sequencing, and resistance genes were identified from assembled sequences. A total of 65 unique resistance genes, plus mutations in two structural resistance loci, were identified. There were more unique resistance genes (n =59) in the 104 human isolates than in the 536 retail meat isolates (n =36). Overall, resistance genotypes and phenotypes correlated in 99.0% of cases. Correlations approached 100% for most classes of antibiotics but were lower for aminoglycosides and beta-lactams. We report the first finding of extended-spectrum β-lactamases (ESBLs) (blaCTX-M1andblaSHV2a) in retail meat isolates ofSalmonellain the United States. Whole-genome sequencing is an effective tool for predicting antibiotic resistance in nontyphoidalSalmonella, although the use of more appropriate surveillance breakpoints and increased knowledge of new resistance alleles will further improve correlations.

scholarly journals Susceptibilidade antimicrobiana de Campylobacter fetus subsp. venerealis isolado de bovinos

2005 ◽  
Vol 25 (1) ◽  
pp. 1-3
Author(s):  
Agueda C. Vargas ◽  
Mateus M. Costa ◽  
Ana C.M. Groff ◽  
Luciane R. Viana ◽  
Cristina C. Krewer ◽  
...  
Keyword(s):  
Campylobacter Fetus ◽  
Fetus Subsp

A campilobacteriose venérea bovina, ocasionada principalmente pelo Campylobacter fetus subsp. fetus e Campylobacter subsp. venerealis, é transmitida através do coito ou por inseminação com sêmen contaminado. O propósito deste estudo foi determinar a susceptibilidade in vitro de isolados de C. fetus subesp. venerealis a agentes antimicrobianos comumente utilizados para o tratamento clínico e de sêmen. Foram testadas duas cepas padrão, sendo uma de C. fetus subsp. fetus e outra de C. fetus subsp. venerealis, bem como 21 amostras de isolados clínicos de C. fetus subsp. venerealis. Os testes foram realizados conforme o método de Kirby-Bauer. A amostra padrão de C. fetus subsp. fetus demonstrou-se resistente à lincomicina, penicilina e ácido nalidíxico, enquanto a de C. fetus subsp. venerealis apresentou susceptibilidade a todos antimicrobianos testados, com exceção do ácido nalidíxico. Todas as amostras de C. fetus subsp. venerealis foram susceptíveis à amicacina, ampicilina, cefalotina, estreptomicina, gentamicina, penicilina e tetraciclina. Foi observada resistência de 42,86% à lincomicina e 4,76 % a enrofloxacina, e de 100% ao ácido nalidíxico. Ainda, 4,76% apresentaram susceptibilidade intermediária à enrofloxacina, neomicina e polimixina B e 9,52% à lincomicina. Os resultados evidenciaram a sensibilidade das amostras analisadas aos antimicrobianos comumente utilizados para o tratamento clínico e do sêmen.

scholarly journals An unusual case of infective spondylodiscitis caused by Campylobacter fetus subsp. fetus: molecular characterization by whole-genome sequencing

2021 ◽  
Vol 3 (7) ◽  
Author(s):  
Dhiviya Prabaa MS ◽  
Jaya Lakshmi SS ◽  
John Antony Jude Prakash ◽  
Kenny S. David ◽  
Vijay Alagar ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Genome Sequencing ◽  
Unusual Case ◽  
Whole Genome ◽  
Content Type ◽  
Link Type ◽  
Campylobacter Fetus ◽  
Adjacent Structures ◽  
Fetus Subsp ◽  
Specific Symptoms

Spondylodiscitis is an infectious inflammation that affects the intervertebral disc and adjacent structures. Treating infective spondylodiscitis is often challenging due to the lack of specific symptoms. Here we present an unusual case of infective spondylodiscitis caused by Campylobacter fetus subsp. fetus .

scholarly journals Conjugal Transfer, Whole-Genome Sequencing, and Plasmid Analysis of Four mcr-1-Bearing Isolates from U.S. Patients

2019 ◽  
Vol 63 (4) ◽  
Author(s):  
Wenming Zhu ◽  
Adrian Lawsin ◽  
Rebecca L. Lindsey ◽  
Dhwani Batra ◽  
Kristen Knipe ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Genome Sequencing ◽  
Resistance Genes ◽  
Whole Genome ◽  
Colistin Resistance ◽  
Content Type ◽  
Plasmid Analysis ◽  
Antimicrobial Resistance Genes

ABSTRACT Four Enterobacteriaceae clinical isolates bearing mcr-1 gene-harboring plasmids were characterized. All isolates demonstrated the ability to transfer colistin resistance to Escherichia coli; plasmids were stable in conjugants after multiple passages on nonselective media. mcr-1 was located on an IncX4 (n = 3) or IncN (n = 1) plasmid. The IncN plasmid harbored 13 additional antimicrobial resistance genes. Results indicate that the mcr-1-bearing plasmids in this study were highly transferable in vitro and stable in the recipients.

scholarly journals Genome Sequence of Campylobacter fetus subsp. venerealis NW_ED23, Isolated from Bovine Sheath Wash

2020 ◽  
Vol 9 (40) ◽  
Author(s):  
Mpinda Edoaurd Tshipamba ◽  
Stephen Abiola Akinola ◽  
Lubanza Ngoma ◽  
Mulunda Mwanza
Keyword(s):  
South Africa ◽  
Genome Sequence ◽  
Causative Agent ◽  
Content Type ◽  
Campylobacter Fetus ◽  
Fetus Subsp ◽  
Bovine Genital Campylobacteriosis

ABSTRACT Campylobacter fetus subsp. venerealis is the causative agent of bovine genital campylobacteriosis, which is mostly characterized by reproduction problems. The strain reported in this study was isolated from bull sheath wash in South Africa.

scholarly journals Prevalence of Tritrichomonas foetus and Campylobacter fetus subsp. venerealis among bulls slaughtered in the state of Minas Gerais, Brazil

2018 ◽  
Vol 39 (5) ◽  
pp. 2039
Author(s):  
Michele Placedino Andrade Botelho ◽  
Christian Hirsch ◽  
Andrey Pereira Lage ◽  
Christiane Maria Barcellos Magalhães da Rocha ◽  
Elaine Maria Seles Dorneles ◽  
...  
Keyword(s):  
Minas Gerais ◽  
Tritrichomonas Foetus ◽  
Sexually Transmitted ◽  
Molecular Tests ◽  
Campylobacter Fetus ◽  
Causative Agents ◽  
Fetus Subsp ◽  
Natural Mating ◽  
Cattle Herds ◽  
Bovine Genital Campylobacteriosis

Bovine genital campylobacteriosis (BGC) and bovine genital trichomonosis (BGT) are globally widespread, predominantly sexually transmitted diseases that mainly affect herds in which natural mating takes place. Bulls serve as good epidemiological indicators for studying the causative agents of these diseases in cattle herds. The objective of the present work was to determine the prevalence of BGC and BGT among bulls sent to slaughterhouses in southern Minas Gerais, Brazil. Samples of preputial smegma from 200 sexually mature, non-castrated bulls of several ancestries were collected in 2013 from four regional slaughterhouses. The polymerase chain reaction (PCR) was employed to detect Tritrichomonas foetus, Campylobacter fetus subsp. fetus, and C. fetus subsp. venerealis. Isolation also was used aiming to diagnosis of BGT. All smegma samples were negative in culture for T. foetus. Molecular tests revealed 8% (16/200) of animals to be positive for T. foetus, 17.5% (35/200) for Campylobacter. fetus subsp. fetus, and 13.5% (27/200) for C. fetus subsp. venerealis. The results of the present study indicate that T. foetus, C. fetus subsp. venerealis, and C. fetus subsp. fetus are present among bulls slaughtered in southern Minas Gerais, and that BGC and BGT occur in this region. These diseases therefore need to be considered during differential diagnosis of reproductive diseases affecting cattle herds in which natural mating is employed.

scholarly journals Natural Horizontal Gene Transfer of Antimicrobial Resistance Genes in Campylobacter spp. From Turkeys and Swine

2021 ◽  
Vol 12 ◽  
Author(s):  
Vanina Guernier-Cambert ◽  
Julian Trachsel ◽  
Joel Maki ◽  
Jing Qi ◽  
Matthew J. Sylte ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Antimicrobial Resistance ◽  
Horizontal Gene Transfer ◽  
Resistance Genes ◽  
Genomic Islands ◽  
Whole Genome ◽  
Antimicrobial Resistance Genes ◽  
Horizontal Spread

Antibiotic-resistant Campylobacter constitutes a serious threat to public health. The clonal expansion of resistant strains and/or the horizontal spread of resistance genes to other strains and species can hinder the clinical effectiveness of antibiotics to treat severe campylobacteriosis. Still, gaps exist in our understanding of the risks of acquisition and spread of antibiotic resistance in Campylobacter. While the in vitro transfer of antimicrobial resistance genes between Campylobacter species via natural transformation has been extensively demonstrated, experimental studies have favored the use of naked DNA to obtain transformants. In this study, we used experimental designs closer to real-world conditions to evaluate the possible transfer of antimicrobial resistance genes between Campylobacter strains of the same or different species (Campylobacter coli or Campylobacter jejuni) and originating from different animal hosts (swine or turkeys). This was evaluated in vitro through co-culture experiments and in vivo with dual-strain inoculation of turkeys, followed by whole genome sequencing of parental and newly emerged strains. In vitro, we observed four independent horizontal gene transfer events leading to the acquisition of resistance to beta-lactams (blaOXA), aminoglycosides [aph(2′′)-If and rpsL] and tetracycline [tet(O)]. Observed events involved the displacement of resistance-associated genes by a mutated version, or the acquisition of genomic islands harboring a resistance determinant by homologous recombination; we did not detect the transfer of resistance-carrying plasmids even though they were present in some strains. In vivo, we recovered a newly emerged strain with dual-resistance pattern and identified the replacement of an existing non-functional tet(O) by a functional tet(O) in the recipient strain. Whole genome comparisons allowed characterization of the events involved in the horizontal spread of resistance genes between Campylobacter following in vitro co-culture and in vivo dual inoculation. Our study also highlights the potential for antimicrobial resistance transfer across Campylobacter species originating from turkeys and swine, which may have implications for farms hosting both species in close proximity.

TheCampylobacter fetusS layer is not essential for initial interaction with HEp-2 cells

1998 ◽  
Vol 44 (3) ◽  
pp. 244-250 ◽  
Author(s):  
Lori L Graham ◽  
K L MacDonald
Keyword(s):  
Wild Type ◽  
Cell Numbers ◽  
Campylobacter Fetus ◽  
Time Period ◽  
Significant Difference ◽  
Mutant Strains ◽  
Fetus Subsp ◽  
Initial Interaction ◽  
Wild Type Parent

In vitro adherence assays were used to determine whether the S layer mediated interactions between Campylobacter fetus subsp. venerealis strains and HEp-2 cells. At multiplicity of infection ratios ranging from 0.1:1 through 100:1, quantitation of bacterial adherence by light microscopy revealed that S layer deficient isogenic C. fetus 809K and C. fetus 810K were not less efficient in their attachment to HEp-2 cells; either S layer deficient C. fetus strains interacted with HEp-2 cells in greater numbers than the corresponding wild-type parent strains 809 and 810 or there was no significant difference in adherence levels between wild-type and mutant strains. Adherence of C. fetus strains to HEp-2 cells increased most during the first 2 h of a 22-h incubation period with only a slight increase in C. fetus cell numbers occuring subsequent to 2 h. At each assay point throughout this 22-h time period, equivalent numbers of wild-type and S layer deficient C. fetus strains were observed associated with HEp-2 cells. Prior to 2 h, adherence levels of all C. fetus strains exceeded those of Escherichia coli AB264 and Salmonella typhimurium SL1344. And, unlike S. typhimurium, C. fetus did not undergo significant replication following initial adherence to HEp-2 cells. Campylobacter fetus did not adhere to HEp-2 cells in a localized or aggregative pattern but were randomly distributed over individual HEp-2 cells and at no time during the assay with C. fetus were changes in HEp-2 cell morphology apparent. These data suggest that the S layer is not essential for mediating initial interactions between C. fetus and HEp-2 cells.Key words: Campylobacter fetus, S layer, HEp-2.

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