scholarly journals Sanitary profile in mice and rat colonies in laboratory animal houses in Minas Gerais: I - Endo and ectoparasites

2007 ◽  
Vol 59 (6) ◽  
pp. 1478-1484 ◽  
Author(s):  
K.A. Bicalho ◽  
F.T.M. Araújo ◽  
R.S. Rocha ◽  
O.S. Carvalho
Keyword(s):  
Physical Environment ◽  
Minas Gerais ◽  
Public Institutions ◽  
Hymenolepis Nana ◽  
Syphacia Muris ◽  
Syphacia Obvelata ◽  
Animal House ◽  
Aspiculuris Tetraptera ◽  
Giardia Muris ◽  
Sanitary Conditions

The sanitary conditions of 13 animal houses in nine public institutions in Minas Gerais, and the presence of endo and ectoparasites of mice and rats colonies kept in these facilities were evaluated. Data about barriers to prevent the transmission of diseases and a program of sanitary monitoring were obtained through a questionnaire and local visit. Parasitological methods were performed for diagnosing mite, lice, helminthes, and protozoa parasites in 344 mice and 111 rats. Data have shown that the majority of the animal houses had neither proper physical environment nor protection barriers to prevent the transmission of infections. Parasitological results have shown that only one animal house (7.7%) had parasite free animals, whereas the others have presented infected animals and the prevalences of parasites in the mice colonies were: Myobia musculi (23.1%); Myocoptes musculinus (38.5%); Radfordia affinis (15.4%); Syphacia obvelata (92.3%); Aspiculuris tetraptera (23.1%); Hymenolepis nana (15.4%); Spironucleus muris (46.2%); Giardia muris (46.2%); Tritrichomonas muris (53.8%); Trichomonas minuta (61.5%); Hexamastix muris (7.7%); and Entamoeba muris (84.6%). As for the rat colonies, the prevalences were: Poliplax spinulosa (8.1%); Syphacia muris (46.2%); Trichosomoides crassicauda (28.6%); Spironucleus muris (85.7%); Tritrichomonas muris (85.7%); Trichomonas minuta (85.7%); Hexamastix muris (14.3%) and Entamoeba muris (85.7%).

scholarly journals Parasite survey in mouse and rat colonies of Brazilian laboratory animal houses kept under differents sanitary barrier conditions

2000 ◽  
Vol 52 (1) ◽  
pp. 33-37 ◽  
Author(s):  
R. Gilioli ◽  
L.A.G. Andrade ◽  
L.A.C. Passos ◽  
F.A. Silva ◽  
D.M. Rodrigues ◽  
...  
Keyword(s):  
Laboratory Animal ◽  
Monitoring Program ◽  
Multiple Infections ◽  
Hymenolepis Nana ◽  
Syphacia Muris ◽  
Syphacia Obvelata ◽  
Aspiculuris Tetraptera ◽  
Giardia Muris ◽  
Sanitary Conditions ◽  
Parasitological Study

A parasitological study was undertaken to determine the health status of 15 mouse and 10 rat colonies bred in 18 Brazilian laboratory animal houses maintained under different sanitary barrier conditions which supply animals for teaching, research purposes and manufacture of biological products for medical or veterinary use. Parasitological methods were used for diagnosis of mites, lices, helminthes and protozoan parasites. A questionnaire was answered by institutions with the intention to obtain information about the existence of barriers against infections and of regular sanitary monitoring program of their colonies. The questionnaire data show that the majority of the animal houses investigated do not possess an efficient sanitary barrier system able to keep animals under controlled health sanitary conditions. Ecto and endoparasite infections are widespread in the colonies and multiple infections were common in animals from most facilities investigated. The prevalences of parasites detected among the mouse and rat colonies of the laboratory animal houses investigated were: Myocoptes musculinus (46.6%), Myobia musculi (26.6%), Radfordia ensifera (13.3%), Syphacia obvelata (86.6%), Aspiculuris tetraptera (60.0%), Hymenolepis nana (53.3%), Spironucleus muris (80.0%), Tritrichomonas muris (80.0%), Giardia muris (66.0%), Entamoeba muris (20.0%), Eimeria sp. (13.3%), Hexamastix muris (26.6%), Poliplax spinulosa (30.0%), Poliplax serrata (10.0%), Radfordia ensifera (30.0%), Syphacia muris (80.0%), Hymenolepis nana (40.0%), Trichosomoides crassicauda (55.5%), Spironucleus muris (90.0%), Tritrichomonas muris (80.0%), Giardia muris (60.0%), Entamoeba muris (80.0%), Eimeria sp. (60.0%) and Hexamastix muris (60.0%).

Continuous feed medication with thiabendazole for the removal of Hymenolepis nana, Syphacia obvelata and Aspiculuris tetraptera in naturally infected mice

1975 ◽  
Vol 49 (3) ◽  
pp. 173-177 ◽  
Author(s):  
L. F. Taffs
Keyword(s):  
High Activity ◽  
Inbred Strain ◽  
Treatment Period ◽  
Day Treatment ◽  
Post Mortem ◽  
Hymenolepis Nana ◽  
Single Drug ◽  
Continuous Feed ◽  
Syphacia Obvelata ◽  
Aspiculuris Tetraptera

AbstractA medicated diet containing 0.3% thiabendazole fed continuously to 26 mice for 7 days removed 9 out of 11 (82%) Hymenolepis nana, (one tapeworm remaining in each of two mice), 17 out of 17 (100%) Syphacia obvelata and 8 out of 8 (100%) Aspiculuris tetraptera infections. By contrast in 26 non-treated mice 9 out of 10 Hymenolepis, 17 out of 18 Syphacia and 8 out of 8 Aspiculuris infections persisted through the 7 day treatment period, and at post mortem examination 38 Hymenolepsis, 1,562 Syphacia and 4,911 Aspiculuris worms were recovered. In a second experiment 100% removal of 23 Hymenolepis and 20 Syphacia infections was obtained after 14 days medication of 38 mice. High activity against both roundyvorms and tapeworms of mice was thus obtained using one single drug. Some unexplained deaths amongst inbred strain C3H/Hef Nimr mice occurred within seven day's of the continuous medication.

scholarly journals A Survey of Helminth Infections in the Black Rat from Stara Zagora District, Bulgaria

2017 ◽  
Vol 40 (2) ◽  
pp. 177-182 ◽  
Author(s):  
Petar T. Iliev ◽  
Georgi Zh. Georgiev ◽  
Zvezdelina T. Kirkova ◽  
Borislava G. Chakarova
Keyword(s):  
Rattus Rattus ◽  
Hymenolepis Diminuta ◽  
Helminth Parasites ◽  
Hymenolepis Nana ◽  
Trichuris Muris ◽  
Black Rat ◽  
Helminth Infections ◽  
Syphacia Obvelata ◽  
Aspiculuris Tetraptera ◽  
Taenia Taeniaeformis

AbstractA field study was conducted to disclose the prevalence of different helminth parasites in the black rat, Rattus rattus, in some regions of Stara Zagora district, Bulgaria. Out of 67 examined rats, 53 (79.1%) were found to be positive. Helminths of three classes were detected such as Cestoda: Hymenolepis diminuta (47.8%), Hymenolepis nana (43.3%), Taenia taeniaeformis larvae (7.5%), Taenia polyacantha larvae (1.5%); Secernentea: Syphacia obvelata (4.5%), Aspiculuris tetraptera (3.0%) and Adenophorea: Capillaria hepatica (9.0%), Trichuris muris (3.0%). Of all the investigated rats, 27 (40.3%) were infected by one helminth species and 26 (38.8%) by more than one. Hymenolepis diminuta was a predominant species in cases of single infections (23.9%). The most frequent co-infections were observed by H. nana and H. diminuta (16.4%).

Effective eradication of pinworms (Syphacia muris, Syphacia obvelata and Aspiculuris tetraptera) from a rodent breeding colony by oral anthelmintic therapy

1998 ◽  
Vol 32 (3) ◽  
pp. 337-342 ◽  
Author(s):  
Lionel Zenner
Keyword(s):  
Toxic Effect ◽  
Tap Water ◽  
Breeding Colony ◽  
The Third ◽  
Hygiene Measures ◽  
Transgenic Lines ◽  
Syphacia Muris ◽  
Acute Toxic Effect ◽  
Syphacia Obvelata ◽  
Aspiculuris Tetraptera

An oral combination of piperazine and ivermectin was used over a 6-week period for treating three different colonies of mice or rats infested with Syphacia obvelata, Syphacia muris or Aspiculuris tetraptera. No acute toxic effect was found in transgenic lines of mice or rats with these products in a preliminary trial. The colonies were treated with piperazine, 2.1 mg/ml in tap water for 2 weeks, then with ivermectin, 0.007 mg/ml, in tap water for the third and fourth weeks, and finally with piperazine for two further weeks. Hygiene measures such as a complete cage change, thorough disinfection and cleaning of the rooms were associated with the treatment. All examinations subsequent to completion of treatment have proved negative for further parasites.

scholarly journals Prevalência de ecto e endoparasitas em camundongos e ratos criados em biotério

1997 ◽  
Vol 34 (3) ◽  
pp. 142
Author(s):  
M. Cecília R. Vieira Bressan ◽  
Gerson Amauri Calgaro ◽  
Sandra R. Alexandre ◽  
Thais Marques
Keyword(s):  
Hymenolepis Nana ◽  
Anal Swab ◽  
Aspiculuris Tetraptera ◽  
Giardia Muris

Durante um período de 5 anos (1986-1991) foram examinados 229 camundongos e 128 ratos, de ambos os sexos, com idade entre 1 a 2 meses, provenientes de 4 biotérios convencionais (2 de camundongos e 2 de ratos), e de um biotério provido de barreiras sanitárias (camundongos controle). A amostragem semestral aleatória consistiu em 5% da população das salas com mais de 100 animais cada. No exame clínico geral observaram-se distensão abdominal e diarréia nos camundongos e ratos dos biotérios convencionais, o que não foi constatado nos animais do biotério controle. Discreta alopecia e pêlos eriçados foram detectados em todos os animais das colônias estudadas. Infestações porMyobia musculi, Myocoptes musculinus, Radfordia affinis, Radfordia ensiferae Poliplax spinulosa foram identificadas nas colônias convencionais, em infestações múltiplas ou simples. Myobia musculiioi descrito pela primeira vez em ratos criados em biotérios. Infecções por endoparasitas  Hymenolepis nana, Syphacia sp, Aspiculuris tetraptera, Trichomonas muris, Spironucleus muris, Giardia muris e Eimeria sp foram observadas nas colônias convencionais. Entretanto, através dos resultados da necrôpsia, na colônia controle de camundongos somente foram  encontrados Syphacia sp e Aspiculuris tetraptera. A necrôpsia confirmou que o uso do método de anal-swab para detecção de ovos de Syphacia sp é preferível ao de Willis, e revelou o grau de infecção de protozoários intestinais. Syphacia sp, Hymenolepis nana e Aspiculuris tetraptera foram eficientemente detectados pelo método de Willis.

scholarly journals OCURRENCIA DE NEMATODOS GASTROINTESTINALES ASPICULURIS TETRAPTERA (NITZSCH, 1821) SCHULZ, 1927 Y SYPHACIA OBVELATA RUDOLPHI, 1802 EN MUS MUSCULUS LINNAEUS, 1758 EN BIOTERIOS DE INVESTIGACION EN MEXICO

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Mayra I Grano-Maldonado
Keyword(s):  
Mus Musculus ◽  
Hymenolepis Nana ◽  
Distribución Geográfica ◽  
Syphacia Obvelata ◽  
Aspiculuris Tetraptera

El ratón común Mus musculus Linnaeus, 1758 es empleado comúnmente como modelo de investigación en las ciencias veterinarias y biomédicas. Cuarenta organismos fueron colectados en cuatro diferentes bioterios de la Universidad Nacional Autónoma de México y fue evaluada la presencia de parásitos. La examinación intestinal reveló al nematodo Aspiculuris tetraptera (Nitzsch, 1821) Schulz, 1927 (n=104) y Syphacia obvelata Rudolphi, 1802 (n=1582). El análisis estadístico determinó que no hay preferencia parasitaria por sexo del hospedero. Se registró al cestodo Rodentolepis nana (Siebold, 1852) sinónimos (Hymenolepis nana y Vampirolepis nana) que son causantes de cestodiasis en el humano. Este trabajo tiene un énfasis en estos helmintos debido a su potencial zoonótico. No se detectó la presencia de ectoparasitos. Se elaboró un reporte del registro de parásitos en roedores de bioterio en México obtenido de una base de datos de la Colección Nacional de Helmintos del Instituto de Biología de la Universidad Nacional Autónoma de México. Este es un primer registro preliminar de la ocurrencia del nematodo A. tetraptera y S. obvelata en el ratón común M. musculus en cuatro bioterios en la ciudad de México. Este trabajo amplía la distribución geográfica y contribuye también, a un nuevo registro del parásito.

Sequence variability in internal transcribed spacers of nuclear ribosomal DNA among isolates of the oxyurid nematodes Syphacia obvelata and Aspiculuris tetraptera from mice reared in laboratories in China

2015 ◽  
Vol 90 (1) ◽  
pp. 81-85 ◽  
Author(s):  
J.H. Qiu ◽  
Y. Lou ◽  
Y. Zhang ◽  
Q.C. Chang ◽  
Z.X. Liu ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Its2 Rdna ◽  
Sequence Variability ◽  
Rdna Sequences ◽  
Syphacia Muris ◽  
Syphacia Obvelata ◽  
Aspiculuris Tetraptera ◽  
Its1 And Its2

AbstractThis study examined sequence variability in internal transcribed spacers (ITS) of nuclear ribosomal DNA among Syphacia obvelata and Aspiculuris tetraptera isolates from laboratory mice from different geographical locations in China. ITS1, 5.8S and ITS2 rDNA were amplified separately from adult S. obvelata and A. tetraptera individuals by polymerase chain reaction (PCR), and the amplicons were subjected to sequencing from both directions. The lengths of the sequences of ITS1, 5.8S and ITS2 rDNA from both nematodes were 314 bp and 456 bp, 157 bp, and 273 bp and 419 bp, respectively. The intraspecific sequence variations in S. obvelata ITS1 were 0–0.3%. For A. tetraptera they were 0–0.7% in ITS1 and 0–1.0% in ITS2. However, the interspecific sequence differences among members of the infraorder Oxyuridomorpha were significantly higher, being 54.0–65.5% for ITS1 and 55.3–64.1% for ITS2. Phylogenetic analysis based on the combined partial sequences of ITS1 and ITS2 using three inference methods – Bayesian inference, maximum likelihood and maximum parsimony – revealed that all the S. obvelata and A. tetraptera samples formed independent monophyletic groups. Syphacia obvelata was closer to Syphacia muris than to A. tetraptera, consistent with morphological classification. These results demonstrate that ITS1 and ITS2 rDNA sequences are useful markers for population genetic studies of oxyurid nematodes.

scholarly journals Parasitocenoses in productional rodent breeds in Czech Republic

2009 ◽  
Vol 57 (1) ◽  
pp. 27-34
Author(s):  
Marie Borkovcová
Keyword(s):  
Czech Republic ◽  
High Mortality ◽  
Clinical Signs ◽  
Hymenolepis Nana ◽  
Regular Control ◽  
Ornithonyssus Bacoti ◽  
Syphacia Obvelata ◽  
Aspiculuris Tetraptera

Aim of this work was to monitor the occurrence of most common parasites of rodents in 13 commercial and hobby breeds. Most often detected protozoans belonged to generaGiardia,EimeriaandCryptosporidium,tapewormsHymenolepis nanaandH. diminuta, nematodsSyphacia obvelataandAspiculuris tetrapteraand mitesOrnithonyssus bacoti,Laelaps hilarisandNotoedres muris.Diseases broke out mainly during summer months. In animals with clinical signs of illnesses there was an expectation of pa­ra­si­te presence, and most of them were nematods – 80%, tapeworms – 45.2%, protozoans – 41.1% and ectoparasites – 22%. Samples of animals without clinical signs of illnesses contained nematods – 16%, tapeworms – 11%, coccidians – 6% and ectoparasites – 0%. Besides evaluation of all samples, breeding conditions were evaluated as well. Consequently plan was made to remove the causes of parasitoses for each monitored breed. Most dangerous parasites were coccidians of the genusCryp­tos­po­ri­dium,which caused high mortality of the young animals. In Czech Republic high percent of breeds are contaminated with parasites, however, there is little experience in how to deal with these illnesses. Results are weak and low-quality breeds, especially of mice and common rats. Important protection is buying animals from well-known and verified breed with no signs of illness and also regular control of excrement samples.

Effective eradication of pinworms (Syphacia obvelata and Aspiculuris tetraptera) with Polygonum cognatum Meissn

2019 ◽  
Vol 196 ◽  
pp. 63-67 ◽  
Author(s):  
Fatma Tuğçe Gürağaç Dereli ◽  
Mert Ilhan ◽  
Esma Kozan ◽  
Esra Küpeli Akkol
Keyword(s):  
Syphacia Obvelata ◽  
Aspiculuris Tetraptera
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