Continuous feed medication with thiabendazole for the removal of Hymenolepis nana, Syphacia obvelata and Aspiculuris tetraptera in naturally infected mice

AbstractA medicated diet containing 0.3% thiabendazole fed continuously to 26 mice for 7 days removed 9 out of 11 (82%) Hymenolepis nana, (one tapeworm remaining in each of two mice), 17 out of 17 (100%) Syphacia obvelata and 8 out of 8 (100%) Aspiculuris tetraptera infections. By contrast in 26 non-treated mice 9 out of 10 Hymenolepis, 17 out of 18 Syphacia and 8 out of 8 Aspiculuris infections persisted through the 7 day treatment period, and at post mortem examination 38 Hymenolepsis, 1,562 Syphacia and 4,911 Aspiculuris worms were recovered. In a second experiment 100% removal of 23 Hymenolepis and 20 Syphacia infections was obtained after 14 days medication of 38 mice. High activity against both roundyvorms and tapeworms of mice was thus obtained using one single drug. Some unexplained deaths amongst inbred strain C3H/Hef Nimr mice occurred within seven day's of the continuous medication.

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Sanitary profile in mice and rat colonies in laboratory animal houses in Minas Gerais: I - Endo and ectoparasites

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/s0102-09352007000600020 ◽

2007 ◽

Vol 59 (6) ◽

pp. 1478-1484 ◽

Cited By ~ 5

Author(s):

K.A. Bicalho ◽

F.T.M. Araújo ◽

R.S. Rocha ◽

O.S. Carvalho

Keyword(s):

Physical Environment ◽

Minas Gerais ◽

Public Institutions ◽

Hymenolepis Nana ◽

Syphacia Muris ◽

Syphacia Obvelata ◽

Animal House ◽

Aspiculuris Tetraptera ◽

Giardia Muris ◽

Sanitary Conditions

The sanitary conditions of 13 animal houses in nine public institutions in Minas Gerais, and the presence of endo and ectoparasites of mice and rats colonies kept in these facilities were evaluated. Data about barriers to prevent the transmission of diseases and a program of sanitary monitoring were obtained through a questionnaire and local visit. Parasitological methods were performed for diagnosing mite, lice, helminthes, and protozoa parasites in 344 mice and 111 rats. Data have shown that the majority of the animal houses had neither proper physical environment nor protection barriers to prevent the transmission of infections. Parasitological results have shown that only one animal house (7.7%) had parasite free animals, whereas the others have presented infected animals and the prevalences of parasites in the mice colonies were: Myobia musculi (23.1%); Myocoptes musculinus (38.5%); Radfordia affinis (15.4%); Syphacia obvelata (92.3%); Aspiculuris tetraptera (23.1%); Hymenolepis nana (15.4%); Spironucleus muris (46.2%); Giardia muris (46.2%); Tritrichomonas muris (53.8%); Trichomonas minuta (61.5%); Hexamastix muris (7.7%); and Entamoeba muris (84.6%). As for the rat colonies, the prevalences were: Poliplax spinulosa (8.1%); Syphacia muris (46.2%); Trichosomoides crassicauda (28.6%); Spironucleus muris (85.7%); Tritrichomonas muris (85.7%); Trichomonas minuta (85.7%); Hexamastix muris (14.3%) and Entamoeba muris (85.7%).

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A Survey of Helminth Infections in the Black Rat from Stara Zagora District, Bulgaria

Macedonian Veterinary Review ◽

10.1515/macvetrev-2017-0021 ◽

2017 ◽

Vol 40 (2) ◽

pp. 177-182 ◽

Cited By ~ 1

Author(s):

Petar T. Iliev ◽

Georgi Zh. Georgiev ◽

Zvezdelina T. Kirkova ◽

Borislava G. Chakarova

Keyword(s):

Rattus Rattus ◽

Hymenolepis Diminuta ◽

Helminth Parasites ◽

Hymenolepis Nana ◽

Trichuris Muris ◽

Black Rat ◽

Helminth Infections ◽

Syphacia Obvelata ◽

Aspiculuris Tetraptera ◽

Taenia Taeniaeformis

AbstractA field study was conducted to disclose the prevalence of different helminth parasites in the black rat, Rattus rattus, in some regions of Stara Zagora district, Bulgaria. Out of 67 examined rats, 53 (79.1%) were found to be positive. Helminths of three classes were detected such as Cestoda: Hymenolepis diminuta (47.8%), Hymenolepis nana (43.3%), Taenia taeniaeformis larvae (7.5%), Taenia polyacantha larvae (1.5%); Secernentea: Syphacia obvelata (4.5%), Aspiculuris tetraptera (3.0%) and Adenophorea: Capillaria hepatica (9.0%), Trichuris muris (3.0%). Of all the investigated rats, 27 (40.3%) were infected by one helminth species and 26 (38.8%) by more than one. Hymenolepis diminuta was a predominant species in cases of single infections (23.9%). The most frequent co-infections were observed by H. nana and H. diminuta (16.4%).

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OCURRENCIA DE NEMATODOS GASTROINTESTINALES ASPICULURIS TETRAPTERA (NITZSCH, 1821) SCHULZ, 1927 Y SYPHACIA OBVELATA RUDOLPHI, 1802 EN MUS MUSCULUS LINNAEUS, 1758 EN BIOTERIOS DE INVESTIGACION EN MEXICO

Neotropical Helminthology ◽

10.24039/rnh201482923 ◽

2021 ◽

Vol 8 (2) ◽

Author(s):

Mayra I Grano-Maldonado

Keyword(s):

Mus Musculus ◽

Hymenolepis Nana ◽

Distribución Geográfica ◽

Syphacia Obvelata ◽

Aspiculuris Tetraptera

El ratón común Mus musculus Linnaeus, 1758 es empleado comúnmente como modelo de investigación en las ciencias veterinarias y biomédicas. Cuarenta organismos fueron colectados en cuatro diferentes bioterios de la Universidad Nacional Autónoma de México y fue evaluada la presencia de parásitos. La examinación intestinal reveló al nematodo Aspiculuris tetraptera (Nitzsch, 1821) Schulz, 1927 (n=104) y Syphacia obvelata Rudolphi, 1802 (n=1582). El análisis estadístico determinó que no hay preferencia parasitaria por sexo del hospedero. Se registró al cestodo Rodentolepis nana (Siebold, 1852) sinónimos (Hymenolepis nana y Vampirolepis nana) que son causantes de cestodiasis en el humano. Este trabajo tiene un énfasis en estos helmintos debido a su potencial zoonótico. No se detectó la presencia de ectoparasitos. Se elaboró un reporte del registro de parásitos en roedores de bioterio en México obtenido de una base de datos de la Colección Nacional de Helmintos del Instituto de Biología de la Universidad Nacional Autónoma de México. Este es un primer registro preliminar de la ocurrencia del nematodo A. tetraptera y S. obvelata en el ratón común M. musculus en cuatro bioterios en la ciudad de México. Este trabajo amplía la distribución geográfica y contribuye también, a un nuevo registro del parásito.

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Parasite survey in mouse and rat colonies of Brazilian laboratory animal houses kept under differents sanitary barrier conditions

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/s0102-09352000000100009 ◽

2000 ◽

Vol 52 (1) ◽

pp. 33-37 ◽

Cited By ~ 11

Author(s):

R. Gilioli ◽

L.A.G. Andrade ◽

L.A.C. Passos ◽

F.A. Silva ◽

D.M. Rodrigues ◽

...

Keyword(s):

Laboratory Animal ◽

Monitoring Program ◽

Multiple Infections ◽

Hymenolepis Nana ◽

Syphacia Muris ◽

Syphacia Obvelata ◽

Aspiculuris Tetraptera ◽

Giardia Muris ◽

Sanitary Conditions ◽

Parasitological Study

A parasitological study was undertaken to determine the health status of 15 mouse and 10 rat colonies bred in 18 Brazilian laboratory animal houses maintained under different sanitary barrier conditions which supply animals for teaching, research purposes and manufacture of biological products for medical or veterinary use. Parasitological methods were used for diagnosis of mites, lices, helminthes and protozoan parasites. A questionnaire was answered by institutions with the intention to obtain information about the existence of barriers against infections and of regular sanitary monitoring program of their colonies. The questionnaire data show that the majority of the animal houses investigated do not possess an efficient sanitary barrier system able to keep animals under controlled health sanitary conditions. Ecto and endoparasite infections are widespread in the colonies and multiple infections were common in animals from most facilities investigated. The prevalences of parasites detected among the mouse and rat colonies of the laboratory animal houses investigated were: Myocoptes musculinus (46.6%), Myobia musculi (26.6%), Radfordia ensifera (13.3%), Syphacia obvelata (86.6%), Aspiculuris tetraptera (60.0%), Hymenolepis nana (53.3%), Spironucleus muris (80.0%), Tritrichomonas muris (80.0%), Giardia muris (66.0%), Entamoeba muris (20.0%), Eimeria sp. (13.3%), Hexamastix muris (26.6%), Poliplax spinulosa (30.0%), Poliplax serrata (10.0%), Radfordia ensifera (30.0%), Syphacia muris (80.0%), Hymenolepis nana (40.0%), Trichosomoides crassicauda (55.5%), Spironucleus muris (90.0%), Tritrichomonas muris (80.0%), Giardia muris (60.0%), Entamoeba muris (80.0%), Eimeria sp. (60.0%) and Hexamastix muris (60.0%).

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Parasitocenoses in productional rodent breeds in Czech Republic

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis ◽

10.11118/actaun200957010027 ◽

2009 ◽

Vol 57 (1) ◽

pp. 27-34

Author(s):

Marie Borkovcová

Keyword(s):

Czech Republic ◽

High Mortality ◽

Clinical Signs ◽

Hymenolepis Nana ◽

Regular Control ◽

Ornithonyssus Bacoti ◽

Syphacia Obvelata ◽

Aspiculuris Tetraptera

Aim of this work was to monitor the occurrence of most common parasites of rodents in 13 commercial and hobby breeds. Most often detected protozoans belonged to generaGiardia,EimeriaandCryptosporidium,tapewormsHymenolepis nanaandH. diminuta, nematodsSyphacia obvelataandAspiculuris tetrapteraand mitesOrnithonyssus bacoti,Laelaps hilarisandNotoedres muris.Diseases broke out mainly during summer months. In animals with clinical signs of illnesses there was an expectation of parasite presence, and most of them were nematods – 80%, tapeworms – 45.2%, protozoans – 41.1% and ectoparasites – 22%. Samples of animals without clinical signs of illnesses contained nematods – 16%, tapeworms – 11%, coccidians – 6% and ectoparasites – 0%. Besides evaluation of all samples, breeding conditions were evaluated as well. Consequently plan was made to remove the causes of parasitoses for each monitored breed. Most dangerous parasites were coccidians of the genusCryptosporidium,which caused high mortality of the young animals. In Czech Republic high percent of breeds are contaminated with parasites, however, there is little experience in how to deal with these illnesses. Results are weak and low-quality breeds, especially of mice and common rats. Important protection is buying animals from well-known and verified breed with no signs of illness and also regular control of excrement samples.

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Influence de la température et de la durée d'un traitement cryoprotecteur sur la résistance au froid de plantules de blé. Étude ultrastructurale des ébauches foliaires

Canadian Journal of Botany ◽

10.1139/b83-110 ◽

1983 ◽

Vol 61 (4) ◽

pp. 1025-1039 ◽

Cited By ~ 5

Author(s):

C. M. Gazeau

Keyword(s):

Endoplasmic Reticulum ◽

Treatment Period ◽

Day Treatment ◽

Leaf Primordia ◽

Ultrastructural Changes ◽

Distilled Water ◽

Wheat Seedlings ◽

Starch Grains ◽

Different Temperatures ◽

Protective Treatment

Wheat seedlings were treated at different temperatures and for various periods of time with a cold-protective substance, composed of a mixture of glycerol, dimethylsulfoxide, and saccharose. When the treatment was done at 20 °C, slight ultrastructural changes appeared in leaf primordia as soon as day 1. Thus numbers of lipid globules increased significantly. When the treatment period was increased to 4 days, numbers of starch grains increased, and there was a marked enlargement of mitochondria and plasts. When the treatment was done at 2 °C, cytoplasmic alterations occurred later than at 20 °C. After a 4-day treatment, they were similar to changes induced at 20 °C. When the treatment period was increased to 12 days, dictyosomes were markedly altered. They clustered close to the nucleus in two or three groups and gave rise to numerous pale vesicles with various shapes and sizes. Around each cluster of such vesicles, there gathered many endoplasmic reticulum vesicles and other organelles (mitochondria, plasts, microbodies, vacuoles). A further cooling of 1 °C/min, down to −15 or −30 °C, enhanced these phenomena. After the seedlings were warmed up to 20 °C in distilled water, the changes induced by the frost-protective treatment and then by freezing were shown to be reversible. [Journal translation]

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Effective eradication of pinworms (Syphacia obvelata and Aspiculuris tetraptera) with Polygonum cognatum Meissn

Experimental Parasitology ◽

10.1016/j.exppara.2018.09.006 ◽

2019 ◽

Vol 196 ◽

pp. 63-67 ◽

Cited By ~ 1

Author(s):

Fatma Tuğçe Gürağaç Dereli ◽

Mert Ilhan ◽

Esma Kozan ◽

Esra Küpeli Akkol

Keyword(s):

Syphacia Obvelata ◽

Aspiculuris Tetraptera

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Enhancement of FSH bioactivity in vivo using site-specific antisera

Journal of Endocrinology ◽

10.1677/joe.0.1520355 ◽

1997 ◽

Vol 152 (3) ◽

pp. 355-363 ◽

Cited By ~ 11

Author(s):

L Ferasin ◽

G Gabai ◽

J Beattie ◽

G Bono ◽

A T Holder

Keyword(s):

Biological Activity ◽

Treatment Period ◽

Ovarian Function ◽

Day Treatment ◽

Site Specific ◽

Dose Dependent Fashion ◽

Snell Dwarf ◽

Fsh Bioactivity ◽

Dose Dependent

The ability of site-specific antipeptide antisera to enhance the biological activity of ovine FSH (oFSH) in vivo was investigated using hypopituitary Snell dwarf mice. These animals were shown to respond to increasing doses of oFSH (3·3–90 μg/day), administered in two daily injections over a 5-day treatment period, in a highly significant dose-dependent fashion. The responses measured were increases in uterine weight, ovarian weight and the index of keratinisation in vaginal smears. The dose-dependent response to oFSH confirmed the suitability of this animal model for these investigations and suggested the suboptimal dose of oFSH (20 μg/day) for use in enhancement studies. Five peptides derived from the β subunit of bovine FSH (bFSH) (A, residues 33–47; B, 40–51; C, 69–80; D, 83–94; E, 27–39) were used to generate polyclonal antipeptide antisera. Of these peptides, only A and B produced an antiserum (raised in sheep) capable of recognising 125I-bFSH in a liquid phase RIA. Antisera prepared against peptide A or peptide B were found to significantly enhance the biological activity of 20 μg oFSH/day over a 5-day treatment period. The response to antipeptide antisera alone did not differ significantly from that observed in PBS-injected control animals, neither did the response to FSH alone differ from that observed in animals treated with FSH plus preimmune serum. Thus the enhanced responses are dependent upon the presence of FSH plus antipeptide antiserum. Peptides A and B are located in a region thought to be involved in receptor recognition, this may have implications for the mechanism underlying this phenomenon and/or the structure/function relationships of FSH. That FSH-enhancing antisera can be generated by immunisation of animals with peptides A and B suggests that it may be possible to develop these peptides as vaccines capable of increasing reproductive performance, such as ovulation rate. The high degree of sequence homology between ovine, bovine and porcine (and to a lesser extent human and equine) FSH in the region covered by peptides A and B suggests that these peptides could also be used to promote and regulate ovarian function in all of these species. Journal of Endocrinology (1997) 152, 355–363

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